Standard name | |
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Human Ortholog | |
Description | Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 6 | 6 | 3 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 9 | 9 | 6 | 1 | 19 | 23 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 3 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 206 | 156 | 192 | 100 | 109 | 154 | 292 | 301 | 211 | 237 | 188 | 266 | 119 | 104 | 137 | 199 | 139 | 144 | 177 | 150 | 210 | 84 | 68 | 59 |
Endoplasmic Reticulum | 3 | 5 | 8 | 3 | 3 | 5 | 5 | 8 | 2 | 0 | 1 | 5 | 5 | 3 | 2 | 14 | 4 | 9 | 1 | 0 | 1 | 8 | 25 | 22 |
Endosome | 1 | 1 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 4 | 0 | 2 | 0 | 7 | 2 | 7 | 1 | 16 | 21 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 3 | 0 | 0 | 2 | 0 | 0 | 3 |
Mitochondria | 2 | 4 | 0 | 1 | 4 | 11 | 30 | 17 | 64 | 78 | 89 | 49 | 3 | 1 | 2 | 2 | 3 | 1 | 2 | 2 | 4 | 1 | 4 | 7 |
Nucleus | 16 | 10 | 27 | 43 | 81 | 33 | 34 | 40 | 15 | 16 | 7 | 22 | 19 | 54 | 32 | 37 | 24 | 43 | 101 | 88 | 107 | 17 | 87 | 83 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 1 | 1 | 5 | 5 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 14 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 1 | 7 | 9 |
Vac/Vac Membrane | 92 | 61 | 138 | 136 | 151 | 72 | 79 | 76 | 48 | 52 | 50 | 52 | 182 | 235 | 219 | 266 | 149 | 168 | 18 | 23 | 51 | 27 | 103 | 101 |
Unique Cell Count | 282 | 220 | 324 | 245 | 308 | 249 | 406 | 412 | 298 | 343 | 298 | 373 | 303 | 361 | 371 | 422 | 265 | 290 | 339 | 286 | 399 | 152 | 360 | 376 |
Labelled Cell Count | 320 | 239 | 370 | 284 | 352 | 277 | 445 | 446 | 346 | 390 | 339 | 402 | 332 | 403 | 397 | 530 | 325 | 368 | 339 | 286 | 399 | 152 | 360 | 376 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.3 | 8.9 | 9.9 | 9.2 | 8.7 | 6.7 | 6.5 | 6.6 | 5.5 | 5.2 | 4.9 | 5.4 | 10.1 | 9.9 | 9.6 | 13.4 | 14.3 | 13.8 | 10.0 | 9.8 | 10.4 |
Std Deviation (1e-4) | 1.8 | 2.1 | 1.3 | 1.4 | 1.3 | 1.1 | 1.1 | 1.1 | 1.1 | 1.0 | 1.0 | 0.9 | 1.6 | 1.7 | 1.7 | 2.7 | 2.7 | 2.4 | 1.6 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.19 | -0.56 | -0.61 | -0.6 | -0.85 | -0.92 | -1.03 | -0.87 | 0.02 | -0.01 | -0.05 | 0.43 | 0.52 | 0.47 | 0.01 | -0.01 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.4 | -6.0 | 0.6 | 3.6 | 4.0 | 3.0 | 2.7 | 1.0 | 3.3 | -5.0 | -8.0 | -5.9 | -3.3 | -1.7 | -2.4 |
Endoplasmic Reticulum | 0 | -1.4 | -0.4 | -1.3 | -0.5 | 0 | 0 | 0 | -1.1 | -0.7 | -1.7 | 0 | 0.7 | -0.8 | 0.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 5.0 | 3.7 | 8.8 | 9.1 | 0 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 3.3 | 6.0 | 1.9 | 0 | 0.6 | -1.6 | -1.9 | -3.3 | -1.3 | -1.0 | 2.7 | 0.1 | 0.2 | 0.3 | 2.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.1 | 1.6 | -3.4 | -6.8 | -7.2 | -7.2 | -7.8 | -7.0 | -8.5 | 4.4 | 5.9 | 4.3 | 5.6 | 3.3 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8622 | 6.3968 | 5.5645 | 5.0263 | 4.5186 | 5.5152 | 5.4091 | 7.3396 | 6.5158 | 6.1828 | 4.9668 | 6.2215 | 4.8617 | 7.7004 | 6.1386 | 6.1478 | 5.6101 | 6.1079 |
Actin | 0.0028 | 0.0001 | 0.0012 | 0.0003 | 0.0001 | 0.0001 | 0.0126 | 0.0003 | 0.0036 | 0.0006 | 0.0007 | 0.0002 | 0.0133 | 0.0002 | 0.0012 | 0.0245 | 0.0031 | 0.0036 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0002 |
Bud Neck | 0.0042 | 0.0005 | 0.0008 | 0.0012 | 0.001 | 0.0017 | 0.0036 | 0.0005 | 0.0008 | 0.0008 | 0.0011 | 0.0016 | 0.0029 | 0.0004 | 0.0013 | 0.0013 | 0.0008 | 0.002 |
Bud Periphery | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0006 | 0 | 0.0003 | 0.0003 | 0.0006 | 0.0001 |
Bud Site | 0.0009 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0035 | 0.0004 | 0.002 | 0.0002 | 0.0004 | 0.0001 | 0.0037 | 0.0003 | 0.0009 | 0.0008 | 0.0002 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.3598 | 0.4646 | 0.3409 | 0.4245 | 0.3779 | 0.3788 | 0.3907 | 0.5723 | 0.3766 | 0.3955 | 0.5116 | 0.3848 | 0.425 | 0.6515 | 0.4898 | 0.6017 | 0.4679 | 0.4942 |
Cytoplasmic Foci | 0.0048 | 0.001 | 0.0031 | 0.0016 | 0.0011 | 0.0014 | 0.0176 | 0.0029 | 0.0017 | 0.0036 | 0.0131 | 0.0027 | 0.0144 | 0.0007 | 0.0077 | 0.0159 | 0.0025 | 0.0056 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0055 | 0.0024 | 0.0026 | 0.0019 | 0.0039 | 0.0021 | 0.0058 | 0.0022 | 0.0023 | 0.0064 | 0.0046 | 0.0034 | 0.0076 | 0.0012 | 0.0024 | 0.0028 | 0.0052 | 0.0031 |
Endosome | 0.0097 | 0.0017 | 0.0084 | 0.0012 | 0.0039 | 0.0021 | 0.0317 | 0.0035 | 0.0035 | 0.0108 | 0.0326 | 0.0039 | 0.0302 | 0.0005 | 0.0093 | 0.009 | 0.0344 | 0.0082 |
Golgi | 0.0008 | 0 | 0.0007 | 0 | 0 | 0 | 0.0059 | 0.0001 | 0.0004 | 0.0005 | 0.0145 | 0.0001 | 0.0102 | 0 | 0.0019 | 0.0037 | 0.0056 | 0.0043 |
Lipid Particles | 0.0016 | 0 | 0.0005 | 0 | 0 | 0 | 0.0049 | 0 | 0.0002 | 0.001 | 0.0197 | 0 | 0.0171 | 0 | 0.0013 | 0.0007 | 0.0033 | 0.0026 |
Mitochondria | 0.0022 | 0.0003 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0074 | 0.0004 | 0.0018 | 0.0013 | 0.0075 | 0.0003 | 0.0064 | 0.0003 | 0.0011 | 0.0015 | 0.0022 | 0.0016 |
None | 0.0096 | 0.0016 | 0.0042 | 0.0056 | 0.0052 | 0.0023 | 0.0025 | 0.0015 | 0.001 | 0.0044 | 0.0017 | 0.001 | 0.0066 | 0.0009 | 0.002 | 0.005 | 0.0011 | 0.002 |
Nuclear Periphery | 0.0205 | 0.0155 | 0.0149 | 0.0139 | 0.0197 | 0.0147 | 0.0214 | 0.0115 | 0.015 | 0.0148 | 0.0191 | 0.0153 | 0.0196 | 0.0092 | 0.0133 | 0.0096 | 0.0233 | 0.0111 |
Nucleolus | 0.0008 | 0.0004 | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0011 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 |
Nucleus | 0.566 | 0.5072 | 0.6101 | 0.5378 | 0.5768 | 0.5908 | 0.4688 | 0.3989 | 0.5841 | 0.5504 | 0.3575 | 0.5804 | 0.4172 | 0.332 | 0.4546 | 0.3063 | 0.4387 | 0.45 |
Peroxisomes | 0.0011 | 0 | 0.0002 | 0 | 0 | 0 | 0.0077 | 0.0001 | 0.0005 | 0.0001 | 0.0023 | 0.0001 | 0.0073 | 0 | 0.0028 | 0.0065 | 0.0002 | 0.0017 |
Punctate Nuclear | 0.006 | 0.0022 | 0.0088 | 0.0103 | 0.0063 | 0.0031 | 0.0042 | 0.0034 | 0.0035 | 0.0027 | 0.0085 | 0.0043 | 0.012 | 0.0015 | 0.0056 | 0.0081 | 0.0052 | 0.0077 |
Vacuole | 0.0026 | 0.0016 | 0.0017 | 0.0006 | 0.0025 | 0.0017 | 0.0069 | 0.0012 | 0.0017 | 0.0042 | 0.0034 | 0.0013 | 0.0028 | 0.0006 | 0.0032 | 0.0013 | 0.0027 | 0.0012 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.002 | 0.0003 | 0.0003 | 0.0019 | 0.0009 | 0.0003 | 0.0009 | 0.0001 | 0.0006 | 0.0003 | 0.0023 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.2261 | 22.2282 | 25.7723 | 31.1588 | 41.3064 | 33.1356 | 30.7859 | 30.0221 | 36.4563 | 37.6762 |
Translational Efficiency | 1.3091 | 1.6866 | 1.2866 | 1.3126 | 1.0116 | 1.1543 | 0.8833 | 0.8433 | 0.8306 | 0.951 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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