ORF | |
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Human Ortholog | |
Description | Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 11 | 9 | 15 | 16 | 15 | 18 | – | 16 | 12 | 13 | 21 | 25 | 31 | 22 | 10 | 11 | 1 | 0 | 3 | 2 | 4 | 12 |
Bud | – | 0 | 2 | 3 | 2 | 3 | 9 | 17 | – | 12 | 11 | 12 | 0 | 4 | 3 | 4 | 1 | 1 | 0 | 0 | 3 | 14 | 19 | 78 |
Bud Neck | – | 0 | 0 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 7 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 8 | 6 | 17 | 8 | 17 | 12 | – | 21 | 16 | 14 | 38 | 60 | 100 | 71 | 41 | 39 | 0 | 0 | 0 | 1 | 4 | 8 |
Cytoplasm | – | 20 | 78 | 89 | 128 | 201 | 232 | 297 | – | 164 | 162 | 223 | 182 | 231 | 235 | 82 | 44 | 50 | 6 | 19 | 24 | 46 | 45 | 44 |
Endoplasmic Reticulum | – | 1 | 66 | 97 | 144 | 190 | 181 | 170 | – | 132 | 108 | 116 | 236 | 328 | 408 | 4 | 1 | 5 | 0 | 0 | 77 | 183 | 243 | 354 |
Endosome | – | 0 | 6 | 7 | 6 | 8 | 14 | 15 | – | 12 | 2 | 1 | 2 | 9 | 11 | 6 | 4 | 4 | 2 | 3 | 14 | 8 | 6 | 12 |
Golgi | – | 0 | 3 | 7 | 3 | 0 | 0 | 1 | – | 2 | 0 | 1 | 1 | 9 | 3 | 57 | 16 | 16 | 0 | 0 | 0 | 3 | 1 | 3 |
Mitochondria | – | 0 | 13 | 13 | 28 | 45 | 96 | 54 | – | 55 | 109 | 101 | 0 | 0 | 0 | 23 | 6 | 10 | 4 | 2 | 9 | 10 | 9 | 15 |
Nucleus | – | 1 | 0 | 0 | 0 | 1 | 0 | 5 | – | 5 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 1 | 2 | 2 | 3 | 7 | 12 | 13 | – | 9 | 14 | 12 | 0 | 1 | 4 | 4 | 4 | 3 | 0 | 0 | 1 | 0 | 1 | 2 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 2 | 1 | 0 | 0 | 2 | 1 | – | 1 | 0 | 0 | 4 | 3 | 7 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 4 | 12 |
Vac/Vac Membrane | – | 6 | 11 | 5 | 16 | 18 | 49 | 105 | – | 63 | 27 | 37 | 7 | 17 | 21 | 86 | 43 | 48 | 4 | 33 | 36 | 49 | 60 | 110 |
Unique Cell Count | 26 | 170 | 202 | 304 | 423 | 522 | 612 | 401 | 369 | 424 | 444 | 611 | 728 | 298 | 139 | 155 | 21 | 64 | 176 | 327 | 410 | 669 | ||
Labelled Cell Count | 29 | 202 | 240 | 362 | 497 | 629 | 710 | 493 | 462 | 531 | 492 | 689 | 829 | 362 | 174 | 187 | 21 | 64 | 176 | 327 | 410 | 669 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 5.4 | 9.0 | 9.6 | 9.1 | 7.8 | 7.0 | 7.7 | – | 7.6 | 7.0 | 7.3 | 12.1 | 12.4 | 12.2 | 16.4 | 16.4 | 16.2 | 13.1 | 13.9 | 12.9 |
Std Deviation (1e-4) | – | 1.0 | 5.1 | 5.5 | 4.1 | 3.3 | 3.1 | 2.9 | – | 2.8 | 2.4 | 2.4 | 5.7 | 6.0 | 5.5 | 4.3 | 4.6 | 4.9 | 6.2 | 6.1 | 6.1 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.0 | -0.21 | -0.37 | -0.24 | – | -0.25 | -0.36 | -0.31 | 0.42 | 0.46 | 0.43 | 0.85 | 0.86 | 0.84 | 0.54 | 0.62 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -0.7 | -1.4 | -2.1 | -2.2 | – | -1.3 | -1.7 | -1.9 | -0.9 | -1.3 | -1.2 | 0.4 | 0.3 | 0.2 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | 0.4 | 0 | -0.9 | 0 | – | 0.3 | -0.2 | -0.8 | 1.6 | 2.1 | 3.3 | 5.3 | 5.9 | 5.2 |
Cytoplasm | -0.4 | -0.8 | 0.4 | -0.3 | 0.6 | – | -1.1 | -0.4 | 1.5 | -1.1 | -1.9 | -3.4 | -4.0 | -2.5 | -2.5 |
Endoplasmic Reticulum | 1.8 | 1.8 | 1.4 | -1.0 | -2.8 | – | -1.4 | -2.2 | -2.7 | 3.2 | 3.4 | 4.0 | -10.9 | -8.1 | -7.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 5.4 | 3.5 | 3.3 |
Mitochondria | -0.5 | 0.6 | 1.1 | 3.3 | 0.5 | – | 2.0 | 5.6 | 4.5 | 0 | 0 | 0 | 0 | -1.2 | -0.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | -0.5 | -1.1 | 1.2 | 3.5 | – | 3.0 | 0.4 | 0.9 | 0 | -2.3 | -2.3 | 5.7 | 5.6 | 5.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7453 | 0.9633 | 0.9757 | 1.1029 | 1.0519 | 1.1506 | 0.8917 | 2.2688 | 1.9086 | 1.7349 | 1.6765 | 1.9696 | -0.754 | 0.0519 | -0.2624 | -0.4106 | -0.0253 | -0.1982 |
Actin | 0.0203 | 0.0003 | 0.0175 | 0.0066 | 0.024 | 0.0011 | 0.0024 | 0.0001 | 0.0013 | 0.0004 | 0.0001 | 0.0021 | 0.0214 | 0.0003 | 0.0033 | 0.0059 | 0.0001 | 0.0001 |
Bud | 0.0037 | 0.0012 | 0.004 | 0.0027 | 0.0005 | 0.0019 | 0.0011 | 0.004 | 0.0021 | 0.0011 | 0.0007 | 0.0007 | 0.0002 | 0.001 | 0.0015 | 0.0015 | 0.0001 | 0.0002 |
Bud Neck | 0.002 | 0.0055 | 0.0062 | 0.0007 | 0.0003 | 0.0037 | 0.0009 | 0.0008 | 0.0006 | 0.0003 | 0.0002 | 0.0012 | 0.0003 | 0.0003 | 0.0015 | 0.0005 | 0.0002 | 0.0005 |
Bud Periphery | 0.0016 | 0.0029 | 0.0043 | 0.0051 | 0.0011 | 0.0016 | 0.0033 | 0.0039 | 0.0035 | 0.0014 | 0.0026 | 0.0012 | 0.0004 | 0.0011 | 0.0019 | 0.0012 | 0.0001 | 0.0004 |
Bud Site | 0.005 | 0.0087 | 0.0094 | 0.0015 | 0.0008 | 0.0005 | 0.0024 | 0.007 | 0.0043 | 0.0008 | 0.0003 | 0.0004 | 0.0006 | 0.0023 | 0.0014 | 0.0005 | 0.0002 | 0.0002 |
Cell Periphery | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0007 | 0.001 | 0.0006 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.1384 | 0.1994 | 0.1728 | 0.1707 | 0.1349 | 0.2715 | 0.1349 | 0.2226 | 0.1559 | 0.0992 | 0.3102 | 0.173 | 0.1842 | 0.2919 | 0.2584 | 0.1602 | 0.1565 | 0.3216 |
Cytoplasmic Foci | 0.0521 | 0.0424 | 0.0488 | 0.0687 | 0.052 | 0.0557 | 0.0567 | 0.052 | 0.0555 | 0.0318 | 0.0281 | 0.0811 | 0.0541 | 0.0636 | 0.0709 | 0.0644 | 0.0381 | 0.0641 |
Eisosomes | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0136 | 0.0078 | 0.0118 | 0.0121 | 0.0124 | 0.0073 | 0.008 | 0.0073 | 0.0091 | 0.0079 | 0.0061 | 0.0094 | 0.0214 | 0.011 | 0.0153 | 0.0099 | 0.0123 | 0.006 |
Endosome | 0.156 | 0.1482 | 0.1536 | 0.2945 | 0.4134 | 0.1998 | 0.2232 | 0.2533 | 0.2661 | 0.4289 | 0.2506 | 0.3482 | 0.2553 | 0.2382 | 0.2774 | 0.2654 | 0.4491 | 0.2927 |
Golgi | 0.009 | 0.003 | 0.0093 | 0.0176 | 0.01 | 0.0048 | 0.0064 | 0.0094 | 0.0085 | 0.0123 | 0.0063 | 0.0186 | 0.0095 | 0.0066 | 0.0088 | 0.0064 | 0.0059 | 0.0044 |
Lipid Particles | 0.0174 | 0.003 | 0.0095 | 0.0037 | 0.0095 | 0.0037 | 0.0138 | 0.0033 | 0.0089 | 0.0046 | 0.0113 | 0.0099 | 0.0111 | 0.0031 | 0.0067 | 0.0019 | 0.0014 | 0.002 |
Mitochondria | 0.0127 | 0.0033 | 0.0062 | 0.0084 | 0.0228 | 0.0039 | 0.0111 | 0.0076 | 0.0094 | 0.0051 | 0.0071 | 0.0105 | 0.008 | 0.0017 | 0.0038 | 0.0031 | 0.0017 | 0.0023 |
None | 0.4617 | 0.4956 | 0.4523 | 0.2791 | 0.2035 | 0.3249 | 0.4087 | 0.2772 | 0.3696 | 0.2764 | 0.1975 | 0.2264 | 0.3077 | 0.2524 | 0.2608 | 0.3315 | 0.2086 | 0.1999 |
Nuclear Periphery | 0.0276 | 0.0056 | 0.0072 | 0.0104 | 0.0124 | 0.0118 | 0.005 | 0.0032 | 0.0054 | 0.0076 | 0.0035 | 0.0038 | 0.0203 | 0.0063 | 0.0075 | 0.0111 | 0.0038 | 0.0095 |
Nucleolus | 0.0029 | 0.0026 | 0.0013 | 0.0009 | 0.0005 | 0.0005 | 0.0011 | 0.003 | 0.0007 | 0.001 | 0.0007 | 0.0004 | 0.0005 | 0.0006 | 0.0004 | 0.0008 | 0.0001 | 0.0002 |
Nucleus | 0.0114 | 0.0048 | 0.005 | 0.0034 | 0.0033 | 0.004 | 0.0029 | 0.0064 | 0.0024 | 0.0055 | 0.0025 | 0.0017 | 0.0026 | 0.0035 | 0.0031 | 0.0053 | 0.0013 | 0.003 |
Peroxisomes | 0.0062 | 0.0007 | 0.0093 | 0.0016 | 0.0043 | 0.0007 | 0.0021 | 0.0008 | 0.0016 | 0.0007 | 0.0006 | 0.0032 | 0.0031 | 0.0013 | 0.0022 | 0.0007 | 0.0002 | 0.0006 |
Punctate Nuclear | 0.0135 | 0.0014 | 0.0026 | 0.0021 | 0.0032 | 0.0019 | 0.0029 | 0.0016 | 0.0011 | 0.0026 | 0.0007 | 0.0012 | 0.0015 | 0.001 | 0.0014 | 0.009 | 0.0003 | 0.0011 |
Vacuole | 0.0367 | 0.0549 | 0.0592 | 0.0871 | 0.0679 | 0.0792 | 0.0959 | 0.1224 | 0.076 | 0.0939 | 0.1383 | 0.0842 | 0.0818 | 0.1014 | 0.0616 | 0.1058 | 0.0897 | 0.0723 |
Vacuole Periphery | 0.0069 | 0.0082 | 0.0092 | 0.0225 | 0.0227 | 0.021 | 0.0163 | 0.0131 | 0.017 | 0.0182 | 0.0317 | 0.0225 | 0.0151 | 0.0119 | 0.0117 | 0.0147 | 0.0303 | 0.0188 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.2162 | 27.9823 | 18.6877 | 14.483 | 13.19 | 14.0782 | 15.2074 | 17.1862 | 13.4566 | 19.9702 |
Translational Efficiency | 1.2084 | 0.7935 | 1.1338 | 1.278 | 1.5337 | 1.4351 | 1.7861 | 1.1801 | 1.6374 | 1.084 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1639 | 1227 | 1673 | 1222 | 1449 | 268 | 510 | 1289 | 3088 | 1495 | 2183 | 2511 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 724.36 | 763.88 | 958.13 | 860.84 | 688.85 | 716.78 | 863.50 | 840.55 | 707.70 | 755.44 | 936.02 | 850.42 |
Standard Deviation | 90.53 | 102.30 | 153.37 | 164.94 | 285.76 | 101.68 | 144.49 | 147.78 | 207.32 | 103.78 | 156.55 | 156.70 |
Intensity Change Log 2 | — | 0.076639 | 0.403515 | 0.249038 | — | 0.057341 | 0.326006 | 0.287144 | — | 0.067265 | 0.366254 | 0.267738 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000125 | 0.000545 | 0.000698 | 0.001229 | 0.000262 | 0.000386 | 0.000680 | 0.001073 | 0.000189 | 0.000517 | 0.000694 | 0.001149 |
Bud Neck | 0.020709 | 0.017455 | 0.011820 | 0.014341 | 0.020080 | 0.022944 | 0.020215 | 0.014164 | 0.020414 | 0.018439 | 0.013781 | 0.014250 |
Bud Site | 0.008083 | 0.010039 | 0.015259 | 0.039519 | 0.005396 | 0.009435 | 0.021120 | 0.027697 | 0.006822 | 0.009930 | 0.016628 | 0.033450 |
Cell Periphery | 0.000416 | 0.000130 | 0.000107 | 0.000271 | 0.000177 | 0.000178 | 0.000163 | 0.000165 | 0.000304 | 0.000139 | 0.000120 | 0.000217 |
Cytoplasm | 0.308952* | 0.341536* | 0.198553* | 0.241826* | 0.380868* | 0.253302* | 0.319942* | 0.311014* | 0.342698* | 0.325719* | 0.226912* | 0.277343* |
Cytoplasmic Foci | 0.144437 | 0.258875* | 0.082768 | 0.101950 | 0.270433* | 0.312138* | 0.067760 | 0.102641 | 0.203559* | 0.268423* | 0.079262 | 0.102305 |
Eisosomes | 0.000146 | 0.000111 | 0.000028 | 0.000048 | 0.000257 | 0.000217 | 0.000060 | 0.000066 | 0.000198 | 0.000130 | 0.000036 | 0.000057 |
Endoplasmic Reticulum | 0.008026 | 0.003997 | 0.014527 | 0.005112 | 0.002843 | 0.002512 | 0.006531 | 0.002294 | 0.005594 | 0.003731 | 0.012659 | 0.003666 |
Endosome | 0.059310 | 0.066551 | 0.102329 | 0.101746 | 0.049148 | 0.063090 | 0.066698 | 0.093697 | 0.054541 | 0.065931 | 0.094005 | 0.097614 |
Golgi | 0.002336 | 0.008641 | 0.009523 | 0.021107 | 0.004427 | 0.004121 | 0.001729 | 0.008007 | 0.003317 | 0.007831 | 0.007702 | 0.014382 |
Lipid Particles | 0.016671 | 0.010561 | 0.008948 | 0.006387 | 0.018015 | 0.020424 | 0.008886 | 0.007020 | 0.017301 | 0.012329 | 0.008934 | 0.006712 |
Mitochondria | 0.003913 | 0.007531 | 0.002587 | 0.003436 | 0.004601 | 0.011173 | 0.002025 | 0.004149 | 0.004236 | 0.008184 | 0.002456 | 0.003802 |
Mitotic Spindle | 0.001809 | 0.005362 | 0.034075 | 0.036008 | 0.001716 | 0.004397 | 0.023988 | 0.019655 | 0.001765 | 0.005189 | 0.031718 | 0.027613 |
None | 0.002530 | 0.001909 | 0.001127 | 0.003261 | 0.001340 | 0.000771 | 0.002952 | 0.001957 | 0.001972 | 0.001705 | 0.001553 | 0.002592 |
Nuclear Periphery | 0.002018 | 0.000616 | 0.007947 | 0.001709 | 0.000759 | 0.001124 | 0.003728 | 0.001164 | 0.001427 | 0.000707 | 0.006962 | 0.001429 |
Nuclear Periphery Foci | 0.000639 | 0.000881 | 0.016613 | 0.006092 | 0.000637 | 0.000941 | 0.007580 | 0.002667 | 0.000638 | 0.000892 | 0.014502 | 0.004334 |
Nucleolus | 0.001832 | 0.001286 | 0.002360 | 0.001968 | 0.001062 | 0.001966 | 0.002631 | 0.002416 | 0.001471 | 0.001408 | 0.002424 | 0.002198 |
Nucleus | 0.119657 | 0.061463 | 0.063055 | 0.057316 | 0.091523 | 0.096686 | 0.184083* | 0.082336 | 0.106456 | 0.067777 | 0.091330 | 0.070160 |
Peroxisomes | 0.003313 | 0.007050 | 0.002510 | 0.007152 | 0.005962 | 0.017864 | 0.003101 | 0.006586 | 0.004556 | 0.008988 | 0.002648 | 0.006861 |
Vacuole | 0.287203* | 0.188349 | 0.405710* | 0.329091* | 0.137393 | 0.172951 | 0.250632* | 0.304776* | 0.216907* | 0.185589 | 0.369480* | 0.316609* |
Vacuole Periphery | 0.007876 | 0.007113 | 0.019455 | 0.020431 | 0.003100 | 0.003380 | 0.005497 | 0.006457 | 0.005635 | 0.006444 | 0.016194 | 0.013258 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.73 | -4.55 | -4.16 | -1.53 | -1.39 | -1.08 | -2.12 | -1.65 | -1.27 | -0.42 | -1.62 | -4.58 | -3.54 | -1.49 | -1.08 |
Bud Neck | 1.82 | 4.80 | 4.18 | 2.17 | -0.15 | -0.80 | -0.02 | 3.44 | 2.62 | 2.11 | 1.31 | 4.19 | 5.41 | 3.13 | 0.97 |
Bud Site | -1.49 | -4.71 | -8.38 | -7.95 | -5.84 | -2.29 | -4.53 | -7.94 | -5.07 | -0.98 | -3.36 | -7.39 | -11.39 | -9.58 | -5.57 |
Cell Periphery | 8.69 | 9.62 | 2.63 | -1.08 | -1.40 | -0.05 | 1.31 | 2.29 | 1.75 | 0.61 | 8.43 | 10.00 | 3.29 | -0.94 | -1.53 |
Cytoplasm | -3.20 | 10.64 | 3.49 | 6.12 | -5.85 | 8.62 | 4.99 | 5.72 | -4.23 | -0.72 | 1.87 | 14.38 | 6.13 | 3.36 | -7.56 |
Cytoplasmic Foci | -15.48 | 13.69 | 10.69 | 24.26 | -2.02 | -2.95 | 32.27 | 28.03 | 17.48 | -6.31 | -9.74 | 30.98 | 26.66 | 29.88 | -4.58 |
Eisosomes | 4.69 | 19.73 | 15.70 | 10.89 | -5.18 | 1.34 | 12.23 | 12.29 | 6.31 | -0.10 | 6.83 | 19.97 | 17.49 | 11.46 | -6.96 |
Endoplasmic Reticulum | 4.64 | -4.89 | 4.06 | -0.35 | 8.01 | 0.53 | -2.66 | 1.82 | 0.66 | 3.38 | 3.03 | -7.13 | 4.76 | 0.89 | 10.08 |
Endosome | -1.64 | -7.70 | -3.34 | -1.88 | 3.16 | -2.07 | -0.03 | -5.22 | -1.66 | -3.81 | -3.35 | -8.12 | -5.96 | -2.42 | 1.69 |
Golgi | -6.22 | -6.27 | -6.38 | -2.83 | -3.10 | 0.36 | 5.77 | -1.36 | -1.27 | -4.63 | -5.15 | -4.33 | -6.28 | -1.80 | -2.92 |
Lipid Particles | 4.62 | 6.10 | 8.54 | 5.41 | 4.11 | -0.53 | 6.39 | 8.73 | 3.56 | 1.29 | 4.16 | 9.05 | 12.14 | 6.31 | 4.16 |
Mitochondria | -3.03 | 2.27 | 1.75 | 4.17 | -0.36 | -1.57 | 4.10 | 1.56 | 1.92 | -1.82 | -3.23 | 3.87 | 2.26 | 4.32 | -1.20 |
Mitotic Spindle | -2.60 | -10.80 | -8.78 | -7.46 | 0.76 | -1.09 | -5.31 | -6.91 | -3.59 | 1.06 | -2.94 | -12.08 | -11.15 | -8.54 | 2.28 |
None | 0.71 | 2.46 | -0.26 | -0.84 | -2.13 | 2.02 | -2.89 | -1.65 | -3.49 | 1.37 | 0.43 | 1.47 | -0.76 | -0.92 | -2.05 |
Nuclear Periphery | 4.74 | -8.61 | 0.15 | -6.26 | 9.00 | -1.07 | -6.46 | -4.37 | -0.89 | 5.18 | 3.95 | -10.64 | -1.41 | -6.61 | 10.33 |
Nuclear Periphery Foci | -2.05 | -12.72 | -6.07 | -5.73 | 7.85 | -1.83 | -4.53 | -4.05 | -3.32 | 2.97 | -2.70 | -13.46 | -7.28 | -6.64 | 9.50 |
Nucleolus | 1.64 | -1.29 | 0.10 | -1.38 | 1.33 | -1.35 | -4.02 | -2.69 | -0.67 | 0.36 | 0.27 | -3.18 | -1.95 | -2.17 | 0.75 |
Nucleus | 11.23 | 8.41 | 8.06 | -2.18 | 0.12 | -0.66 | -9.39 | 0.68 | 1.00 | 9.45 | 9.51 | 0.83 | 6.48 | -2.97 | 4.48 |
Peroxisomes | -4.65 | 2.05 | -3.03 | 1.11 | -4.42 | -3.54 | 4.68 | 0.99 | 3.86 | -4.18 | -4.69 | 5.12 | -1.48 | 3.55 | -6.21 |
Vacuole | 10.84 | -11.52 | -6.46 | -16.40 | 4.08 | -3.02 | -11.31 | -21.94 | -11.82 | -3.75 | 4.37 | -20.52 | -17.97 | -20.48 | 3.17 |
Vacuole Periphery | 1.08 | -7.89 | -3.83 | -4.26 | 1.40 | -0.34 | -0.93 | -2.80 | -1.79 | -1.08 | -1.11 | -9.56 | -4.30 | -3.10 | 3.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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