Standard name
Human Ortholog
Description Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.94 0.95 0.87 – 0.68 0.66 0.65 0.66 0.58 0.97 0.99 0.99 0.91 0.91 0.95 0.95 0.95 0.88 0.86 0.86 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.16 0.06 0.27 – 0.3 0.42 0.38 0.42 0.52 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.13 0.11 0.12 0.05 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 1 1 2 0 3 2
Bud 2 1 2 3 1 0 – 5 6 2 10 9 0 0 3 0 0 0 4 0 3 3 5 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 1 0 0 0 0 0 – 1 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 5 0 0 1 5 – 10 5 6 3 10 1 0 0 1 1 1 0 0 0 0 0 0
Cytoplasm 533 415 126 304 310 301 – 461 323 362 296 285 304 513 605 52 21 42 530 406 123 112 326 370
Endoplasmic Reticulum 0 0 0 0 2 0 – 1 0 3 2 4 1 2 2 3 0 2 0 0 1 3 4 5
Endosome 0 0 0 0 1 0 – 2 1 2 1 0 0 1 2 0 0 1 4 3 0 0 9 9
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 4 6
Mitochondria 6 2 6 52 20 92 – 205 207 212 186 258 1 0 0 2 1 2 4 2 0 0 4 2
Nucleus 0 0 0 1 1 2 – 12 8 7 10 11 2 1 0 2 0 0 1 3 0 2 0 0
Nuclear Periphery 0 1 1 1 0 0 – 2 3 5 2 5 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 3 2
Vac/Vac Membrane 6 4 0 3 10 15 – 87 52 67 24 32 7 0 4 2 0 2 5 4 3 2 6 6
Unique Cell Count 544 419 130 324 327 345 676 490 554 447 493 314 516 614 57 23 44 561 426 140 130 378 419
Labelled Cell Count 550 428 135 364 346 415 787 605 668 535 614 316 517 617 63 23 50 561 426 140 130 378 419


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.6 4.1 4.8 6.1 4.9 – 5.3 4.6 5.1 5.0 5.0 6.1 6.5 6.8 7.5 8.6 7.9 5.4 6.6 6.7
Std Deviation (1e-4) 0.6 0.8 1.0 1.2 1.9 1.1 – 2.1 1.0 1.2 1.2 1.2 1.3 1.2 1.3 1.2 1.9 1.5 1.4 2.2 2.4
Intensity Change (Log2) – – – 0.23 0.57 0.26 – 0.39 0.19 0.33 0.3 0.29 0.59 0.68 0.73 0.89 1.08 0.95 0.41 0.69 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -1.0 -3.1 – -6.7 -7.0 -7.2 -7.0 -8.4 -0.1 2.5 1.3 -1.7 -1.3 -0.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.6 0 – 6.1 0 7.4 7.9 9.8 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 4.3 3.9 4.2 2.7 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0592 0.012 0.0144 0.0034 0.002 0.0133 0.043 0.0007 0.0242 0.0437 0.0483 0.0015 – – – – – –
Bud 0.0014 0.0005 0.0006 0.0017 0.0003 0.0002 0.0005 0.0001 0.0007 0.0004 0.0005 0.0002 – – – – – –
Bud Neck 0.0011 0.0003 0.0006 0.0008 0.0004 0.0007 0.0021 0.0001 0.0033 0.0007 0.0008 0.0016 – – – – – –
Bud Periphery 0.0022 0.0003 0.0004 0.001 0.0002 0.0003 0.0005 0 0.0008 0.0004 0.0008 0.0001 – – – – – –
Bud Site 0.0047 0.0058 0.0062 0.0023 0.0018 0.0003 0.0017 0.0004 0.0048 0.0005 0.0007 0.0001 – – – – – –
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 – – – – – –
Cytoplasm 0.6282 0.9223 0.8892 0.815 0.7599 0.8791 0.8055 0.9577 0.8634 0.8268 0.7444 0.933 – – – – – –
Cytoplasmic Foci 0.0403 0.0077 0.0293 0.0315 0.0104 0.0189 0.0247 0.0033 0.0142 0.0224 0.0219 0.008 – – – – – –
Eisosomes 0.0005 0.0001 0 0 0 0.0001 0.0002 0 0.0005 0.0001 0.0007 0 – – – – – –
Endoplasmic Reticulum 0.0204 0.002 0.0047 0.0175 0.0253 0.0042 0.0043 0.0028 0.0031 0.0047 0.007 0.0023 – – – – – –
Endosome 0.081 0.0022 0.0149 0.0667 0.1261 0.0243 0.0121 0.002 0.0127 0.0311 0.0476 0.0062 – – – – – –
Golgi 0.0202 0.0011 0.0039 0.0188 0.0048 0.0135 0.0059 0.0001 0.0051 0.0095 0.0195 0.0007 – – – – – –
Lipid Particles 0.0264 0.0034 0.0041 0.0014 0.0038 0.0095 0.0042 0.0001 0.0056 0.0202 0.0164 0.0003 – – – – – –
Mitochondria 0.0113 0.0012 0.001 0.012 0.0017 0.0032 0.0022 0.0001 0.0045 0.0037 0.0139 0.0003 – – – – – –
None 0.0599 0.0294 0.0079 0.0027 0.0076 0.0212 0.0533 0.0275 0.0201 0.0152 0.0101 0.0167 – – – – – –
Nuclear Periphery 0.0128 0.0014 0.0016 0.0052 0.021 0.0024 0.0034 0.001 0.0032 0.004 0.018 0.0021 – – – – – –
Nucleolus 0.0011 0 0.0001 0.0001 0.0002 0.0001 0.0008 0 0.0004 0.0001 0.001 0.0001 – – – – – –
Nucleus 0.0067 0.0068 0.0073 0.0107 0.0178 0.0051 0.0139 0.0027 0.0208 0.0058 0.0172 0.0222 – – – – – –
Peroxisomes 0.0046 0.002 0.0109 0.0033 0.0003 0.0008 0.008 0.0001 0.0063 0.0046 0.0186 0.0003 – – – – – –
Punctate Nuclear 0.0065 0.0007 0.0019 0.0008 0.0026 0.0011 0.0124 0.0004 0.0046 0.0012 0.0049 0.0034 – – – – – –
Vacuole 0.0072 0.0007 0.0008 0.0035 0.0102 0.0012 0.0011 0.0008 0.0012 0.0035 0.0049 0.0007 – – – – – –
Vacuole Periphery 0.0038 0.0001 0.0002 0.0015 0.0035 0.0005 0.0003 0.0001 0.0004 0.0013 0.0027 0.0002 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 147.1206 84.5465 90.6204 138.6263 120.3531 126.0336 132.9765 137.8307 161.7401 145.8182
Translational Efficiency 2.9231 3.9537 3.5045 2.6407 2.6849 3.6187 2.863 2.4227 2.2811 2.2647

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
246 927 169 142 2022 1570 1865 1643 2268 2497 2034 1785

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 769.25 776.07 1198.56 1136.50 734.57 784.21 975.70 942.16 738.33 781.19 994.22 957.62
Standard Deviation 1140.98 104.58 144.06 159.94 85.15 102.27 153.33 124.88 384.43 103.21 164.51 138.40
Intensity Change Log 2 — 0.012734 0.639778 0.563073 — 0.094340 0.409538 0.359072 — 0.053173 0.531893 0.467022

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000314 0.000639 0.000779 0.020125 0.000163 0.000306 0.000180 0.001262 0.000179 0.000429 0.000230 0.002762
Bud Neck 0.035577 0.017636 0.002858 0.002616 0.012409 0.023709 0.004265 0.005713 0.014922 0.021454 0.004149 0.005467
Bud Site 0.009431 0.016400 0.004642 0.046140 0.005602 0.015634 0.006159 0.038665 0.006017 0.015918 0.006033 0.039260
Cell Periphery 0.000640 0.000112 0.000222 0.000137 0.000111 0.000097 0.000051 0.000133 0.000169 0.000103 0.000065 0.000133
Cytoplasm 0.374583* 0.526606* 0.509524* 0.384415* 0.536969* 0.547434* 0.700835* 0.624805* 0.519356* 0.539702* 0.684939* 0.605681*
Cytoplasmic Foci 0.375233* 0.222166* 0.013446 0.016204 0.213297* 0.216254* 0.006980 0.026337 0.230861* 0.218449* 0.007518 0.025531
Eisosomes 0.000295 0.000094 0.000037 0.000053 0.000148 0.000088 0.000007 0.000030 0.000164 0.000090 0.000010 0.000032
Endoplasmic Reticulum 0.000528 0.000809 0.005964 0.002588 0.000877 0.000464 0.000781 0.001042 0.000839 0.000592 0.001212 0.001165
Endosome 0.018984 0.018230 0.003112 0.004928 0.009308 0.018260 0.000772 0.005849 0.010358 0.018249 0.000967 0.005776
Golgi 0.007009 0.004334 0.000187 0.000688 0.003192 0.004782 0.000115 0.002465 0.003606 0.004616 0.000121 0.002324
Lipid Particles 0.009855 0.003351 0.001690 0.003022 0.005993 0.004001 0.000317 0.000799 0.006412 0.003760 0.000431 0.000975
Mitochondria 0.007596 0.003898 0.000375 0.000475 0.004364 0.009108 0.000326 0.004776 0.004715 0.007174 0.000330 0.004434
Mitotic Spindle 0.000176 0.004383 0.002717 0.025164 0.002815 0.003596 0.003001 0.037064 0.002528 0.003888 0.002978 0.036117
None 0.001526 0.002629 0.005731 0.003341 0.001797 0.002679 0.007018 0.004005 0.001767 0.002660 0.006911 0.003952
Nuclear Periphery 0.000740 0.000330 0.001471 0.001930 0.000365 0.000168 0.000437 0.000647 0.000405 0.000228 0.000523 0.000749
Nuclear Periphery Foci 0.000431 0.000865 0.006062 0.008478 0.000267 0.000399 0.001575 0.001440 0.000285 0.000572 0.001948 0.002000
Nucleolus 0.001180 0.001009 0.000241 0.000201 0.000528 0.001604 0.000183 0.000310 0.000599 0.001383 0.000187 0.000301
Nucleus 0.092989 0.077506 0.335074* 0.107132 0.146252* 0.070409 0.234795* 0.117380 0.140475* 0.073043 0.243127* 0.116565
Peroxisomes 0.016601 0.010852 0.000205 0.036316 0.002854 0.007707 0.000194 0.001010 0.004345 0.008874 0.000195 0.003819
Vacuole 0.043036 0.086346 0.105473 0.335867* 0.051740 0.070896 0.031874 0.124811 0.050796 0.076632 0.037989 0.141601
Vacuole Periphery 0.003274 0.001808 0.000191 0.000179 0.000952 0.002405 0.000134 0.001458 0.001204 0.002183 0.000139 0.001356

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.04 -4.47 -1.14 -0.89 -0.59 -1.78 -1.27 -2.37 -1.54 -2.03 -3.13 -2.28 -2.59 -1.27 -1.98
Bud Neck 2.67 4.77 4.80 7.20 0.30 -5.80 7.90 9.82 10.82 -0.17 -3.87 9.05 10.34 12.79 -0.15
Bud Site -2.72 1.34 -0.30 1.54 -1.19 -6.77 0.19 -5.44 -0.54 -5.42 -8.25 0.52 -5.36 -0.27 -5.53
Cell Periphery 1.44 0.99 1.37 -1.78 4.33 2.15 9.66 2.37 1.07 -2.08 1.65 2.19 1.93 1.25 -0.84
Cytoplasm -7.82 -1.28 5.03 13.55 5.72 -0.95 -11.86 4.08 4.48 14.70 -2.23 -11.97 4.45 6.32 15.12
Cytoplasmic Foci 9.04 22.93 23.24 30.79 2.12 -0.32 46.69 43.51 37.13 -8.95 2.24 50.51 47.66 47.25 -8.51
Eisosomes 6.82 8.44 8.12 3.43 -1.59 7.71 20.80 17.04 12.44 -17.95 9.48 22.27 18.80 12.84 -16.33
Endoplasmic Reticulum -2.36 -5.59 -9.69 -8.98 3.27 3.14 -1.58 -4.40 -10.78 -4.20 2.06 -4.71 -6.16 -12.09 -0.23
Endosome 0.25 5.73 5.68 9.46 0.02 -7.25 16.20 10.32 13.25 -4.76 -7.60 16.86 11.69 16.38 -4.67
Golgi 1.47 3.81 3.83 6.17 0.66 -2.66 8.87 4.86 6.74 -1.97 -1.77 9.55 5.85 7.59 -1.96
Lipid Particles 5.03 5.86 5.22 0.76 -1.35 2.43 13.30 11.94 4.27 -10.53 4.41 14.61 13.16 5.64 -8.64
Mitochondria 1.61 3.18 3.20 3.71 0.49 -2.87 5.30 2.62 4.64 -2.43 -1.94 6.07 3.31 4.83 -2.42
Mitotic Spindle -4.66 -2.71 -3.26 -2.66 -2.71 -0.65 -1.04 -6.98 -6.71 -6.81 -1.59 -1.51 -7.64 -7.11 -7.29
None -1.73 -3.33 -3.38 -2.22 -0.38 -1.77 -17.42 -8.15 -2.78 6.97 -2.34 -17.73 -8.82 -3.78 6.52
Nuclear Periphery 0.86 -2.73 -4.87 -9.94 -3.92 1.81 -3.29 -9.73 -19.68 -11.38 1.56 -3.89 -10.50 -20.21 -11.76
Nuclear Periphery Foci -2.76 -7.61 -5.12 -4.74 -0.55 -1.57 -10.83 -8.56 -3.57 1.41 -3.09 -12.83 -9.59 -5.60 0.90
Nucleolus 0.39 2.29 2.04 4.45 -1.71 -2.86 8.72 4.76 3.38 -2.63 -3.18 7.15 4.74 4.29 -2.80
Nucleus 1.83 -13.35 -5.12 -6.50 7.58 17.70 -21.36 -6.93 -21.91 13.12 17.70 -24.55 -8.31 -23.04 14.80
Peroxisomes 1.64 4.83 3.35 3.06 -0.97 -5.57 8.62 7.72 9.18 -1.77 -5.53 8.99 7.49 12.25 -1.74
Vacuole -7.39 -7.74 -17.37 -15.58 -10.80 -6.49 -4.08 -28.92 -24.29 -25.25 -10.27 -6.76 -32.03 -26.66 -26.52
Vacuole Periphery 0.71 1.46 1.49 3.45 2.68 -4.21 7.91 2.46 5.28 -2.92 -2.60 4.42 2.57 5.58 -2.87
Standard name
Human Ortholog
Description Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ecm15

Ecm15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available