Standard name
Human Ortholog
Description Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.94 0.95 0.87 – 0.68 0.66 0.65 0.66 0.58 0.97 0.99 0.99 0.91 0.91 0.95 0.95 0.95 0.88 0.86 0.86 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.16 0.06 0.27 – 0.3 0.42 0.38 0.42 0.52 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.13 0.11 0.12 0.05 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 1 1 2 0 3 2
Bud 2 1 2 3 1 0 – 5 6 2 10 9 0 0 3 0 0 0 4 0 3 3 5 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 1 0 0 0 0 0 – 1 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 5 0 0 1 5 – 10 5 6 3 10 1 0 0 1 1 1 0 0 0 0 0 0
Cytoplasm 533 415 126 304 310 301 – 461 323 362 296 285 304 513 605 52 21 42 530 406 123 112 326 370
Endoplasmic Reticulum 0 0 0 0 2 0 – 1 0 3 2 4 1 2 2 3 0 2 0 0 1 3 4 5
Endosome 0 0 0 0 1 0 – 2 1 2 1 0 0 1 2 0 0 1 4 3 0 0 9 9
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 4 6
Mitochondria 6 2 6 52 20 92 – 205 207 212 186 258 1 0 0 2 1 2 4 2 0 0 4 2
Nucleus 0 0 0 1 1 2 – 12 8 7 10 11 2 1 0 2 0 0 1 3 0 2 0 0
Nuclear Periphery 0 1 1 1 0 0 – 2 3 5 2 5 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 3 2
Vac/Vac Membrane 6 4 0 3 10 15 – 87 52 67 24 32 7 0 4 2 0 2 5 4 3 2 6 6
Unique Cell Count 544 419 130 324 327 345 676 490 554 447 493 314 516 614 57 23 44 561 426 140 130 378 419
Labelled Cell Count 550 428 135 364 346 415 787 605 668 535 614 316 517 617 63 23 50 561 426 140 130 378 419


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.6 4.1 4.8 6.1 4.9 – 5.3 4.6 5.1 5.0 5.0 6.1 6.5 6.8 7.5 8.6 7.9 5.4 6.6 6.7
Std Deviation (1e-4) 0.6 0.8 1.0 1.2 1.9 1.1 – 2.1 1.0 1.2 1.2 1.2 1.3 1.2 1.3 1.2 1.9 1.5 1.4 2.2 2.4
Intensity Change (Log2) – – – 0.23 0.57 0.26 – 0.39 0.19 0.33 0.3 0.29 0.59 0.68 0.73 0.89 1.08 0.95 0.41 0.69 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -1.0 -3.1 – -6.7 -7.0 -7.2 -7.0 -8.4 -0.1 2.5 1.3 -1.7 -1.3 -0.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.6 0 – 6.1 0 7.4 7.9 9.8 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 4.3 3.9 4.2 2.7 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0592 0.012 0.0144 0.0034 0.002 0.0133 0.043 0.0007 0.0242 0.0437 0.0483 0.0015 – – – – – –
Bud 0.0014 0.0005 0.0006 0.0017 0.0003 0.0002 0.0005 0.0001 0.0007 0.0004 0.0005 0.0002 – – – – – –
Bud Neck 0.0011 0.0003 0.0006 0.0008 0.0004 0.0007 0.0021 0.0001 0.0033 0.0007 0.0008 0.0016 – – – – – –
Bud Periphery 0.0022 0.0003 0.0004 0.001 0.0002 0.0003 0.0005 0 0.0008 0.0004 0.0008 0.0001 – – – – – –
Bud Site 0.0047 0.0058 0.0062 0.0023 0.0018 0.0003 0.0017 0.0004 0.0048 0.0005 0.0007 0.0001 – – – – – –
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 – – – – – –
Cytoplasm 0.6282 0.9223 0.8892 0.815 0.7599 0.8791 0.8055 0.9577 0.8634 0.8268 0.7444 0.933 – – – – – –
Cytoplasmic Foci 0.0403 0.0077 0.0293 0.0315 0.0104 0.0189 0.0247 0.0033 0.0142 0.0224 0.0219 0.008 – – – – – –
Eisosomes 0.0005 0.0001 0 0 0 0.0001 0.0002 0 0.0005 0.0001 0.0007 0 – – – – – –
Endoplasmic Reticulum 0.0204 0.002 0.0047 0.0175 0.0253 0.0042 0.0043 0.0028 0.0031 0.0047 0.007 0.0023 – – – – – –
Endosome 0.081 0.0022 0.0149 0.0667 0.1261 0.0243 0.0121 0.002 0.0127 0.0311 0.0476 0.0062 – – – – – –
Golgi 0.0202 0.0011 0.0039 0.0188 0.0048 0.0135 0.0059 0.0001 0.0051 0.0095 0.0195 0.0007 – – – – – –
Lipid Particles 0.0264 0.0034 0.0041 0.0014 0.0038 0.0095 0.0042 0.0001 0.0056 0.0202 0.0164 0.0003 – – – – – –
Mitochondria 0.0113 0.0012 0.001 0.012 0.0017 0.0032 0.0022 0.0001 0.0045 0.0037 0.0139 0.0003 – – – – – –
None 0.0599 0.0294 0.0079 0.0027 0.0076 0.0212 0.0533 0.0275 0.0201 0.0152 0.0101 0.0167 – – – – – –
Nuclear Periphery 0.0128 0.0014 0.0016 0.0052 0.021 0.0024 0.0034 0.001 0.0032 0.004 0.018 0.0021 – – – – – –
Nucleolus 0.0011 0 0.0001 0.0001 0.0002 0.0001 0.0008 0 0.0004 0.0001 0.001 0.0001 – – – – – –
Nucleus 0.0067 0.0068 0.0073 0.0107 0.0178 0.0051 0.0139 0.0027 0.0208 0.0058 0.0172 0.0222 – – – – – –
Peroxisomes 0.0046 0.002 0.0109 0.0033 0.0003 0.0008 0.008 0.0001 0.0063 0.0046 0.0186 0.0003 – – – – – –
Punctate Nuclear 0.0065 0.0007 0.0019 0.0008 0.0026 0.0011 0.0124 0.0004 0.0046 0.0012 0.0049 0.0034 – – – – – –
Vacuole 0.0072 0.0007 0.0008 0.0035 0.0102 0.0012 0.0011 0.0008 0.0012 0.0035 0.0049 0.0007 – – – – – –
Vacuole Periphery 0.0038 0.0001 0.0002 0.0015 0.0035 0.0005 0.0003 0.0001 0.0004 0.0013 0.0027 0.0002 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 147.1206 84.5465 90.6204 138.6263 120.3531 126.0336 132.9765 137.8307 161.7401 145.8182
Translational Efficiency 2.9231 3.9537 3.5045 2.6407 2.6849 3.6187 2.863 2.4227 2.2811 2.2647

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ecm15

Ecm15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available