Standard name
Human Ortholog
Description Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.27 0.14 0.09 0.14 0.13 0.18 – 0.35 0.42 0.4 0.77 0.32 0.16 0.1 0.07 0.38 0.34 0.36 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.09 0.12 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.09 0.38 0.2 0.09 0.14 – 0.19 0.11 0.22 0.13 0.18 0.14 0.16 0.13 0.13 0 0.15 0 0 0 0 0 0
Nucleus 0.9 0.89 0.79 0.86 0.88 0.8 – 0.73 0.67 0.7 0.2 0.76 0.87 0.82 0.79 0.72 0.66 0.61 0.85 0.84 0.63 0.72 0.73 0.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0.11 0.19 0.38 0.24 0.13 0.24 – 0.24 0.18 0.29 0.16 0.27 0.22 0.23 0.22 0.15 0.2 0.21 0 0.05 0.21 0.17 0.13 0.22
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 1 3 0 11 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 80 18 14 42 43 69 – 222 177 127 372 106 40 31 21 55 19 26 0 0 0 0 0 4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 13 5 9 1 1 0 0 1 0
Endosome 0 0 0 1 0 0 – 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Mitochondria 32 12 57 57 29 53 – 120 46 70 62 59 34 48 38 19 1 11 0 0 0 1 2 2
Nucleus 264 118 120 249 291 311 – 465 286 221 96 248 213 250 232 105 37 44 242 119 84 81 177 199
Nuclear Periphery 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 14 5 1 2 8 13
Nucleolus 33 25 58 69 44 95 – 151 77 91 76 87 54 70 65 22 11 15 12 7 28 19 31 75
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 0 – 0 0 0 0 0 1 0 4 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 0 0 2 0 2 – 7 4 1 5 4 0 4 1 2 2 0 1 1 1 1 3 11
Unique Cell Count 294 132 152 290 330 388 639 424 315 485 328 244 306 294 145 56 72 284 143 134 114 242 336
Labelled Cell Count 411 174 249 420 408 530 968 594 510 624 507 344 403 362 216 75 105 284 143 134 114 242 336


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 185.7 162.4 113.2 139.4 156.9 141.8 – 163.5 164.4 150.8 268.4 148.6 157.1 150.1 136.6 191.0 161.8 169.1 160.3 161.4 142.5
Std Deviation (1e-4) 50.3 60.5 46.0 59.0 55.2 56.1 – 63.0 70.4 63.3 150.9 63.0 53.9 56.6 52.4 71.8 86.4 74.7 51.5 58.7 57.1
Intensity Change (Log2) – – – 0.3 0.47 0.32 – 0.53 0.54 0.41 1.25 0.39 0.47 0.41 0.27 0.75 0.52 0.58 0.5 0.51 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 1.2 2.5 – 6.2 7.3 6.9 14.9 5.4 2.0 0.3 -0.8 5.9 4.3 4.9
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 3.8 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.1 -7.7 -6.2 – -5.0 -7.4 -3.5 -6.8 -4.6 -5.4 -5.2 -6.0 -4.8 -5.1 -3.4
Nucleus 1.9 2.7 0.3 – -1.6 -2.7 -2.0 -13.4 -0.8 2.2 0.7 0 -1.3 -1.9 -2.8
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.2 -6.2 -3.2 – -3.7 -5.0 -2.0 -5.9 -2.6 -3.4 -3.4 -3.6 -4.5 -2.5 -2.6
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0 0 0 0 0 0.0004 0 0 0.0011 0 0.0001 0 – – – – – –
Bud 0.0001 0 0 0 0.0001 0.0001 0 0 0.0003 0 0.0001 0 – – – – – –
Bud Neck 0 0 0 0 0 0 0 0 0.0002 0 0 0 – – – – – –
Bud Periphery 0.0001 0 0 0 0.0001 0.0001 0 0 0.0007 0 0.0001 0 – – – – – –
Bud Site 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0.0026 0 0.0001 0 – – – – – –
Cell Periphery 0.0002 0.0001 0 0 0.0006 0.0002 0.0001 0 0.0004 0 0.001 0.0001 – – – – – –
Cytoplasm 0 0 0 0 0 0.002 0 0 0.0003 0 0 0 – – – – – –
Cytoplasmic Foci 0 0 0 0 0.0001 0.0002 0 0 0.0001 0 0 0 – – – – – –
Eisosomes 0 0 0 0 0.0002 0 0 0 0.0001 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 0.0013 0 0 0.0003 0 0 0 – – – – – –
Endosome 0 0 0 0 0 0.003 0 0 0.0003 0 0 0 – – – – – –
Golgi 0 0 0 0 0 0.0001 0 0 0.0001 0 0 0 – – – – – –
Lipid Particles 0.0001 0 0 0 0.0001 0 0 0 0.0001 0 0.0005 0 – – – – – –
Mitochondria 0.0076 0.0021 0.0022 0.0016 0.1029 0.0017 0.0027 0.0005 0.0026 0.0015 0.097 0.0007 – – – – – –
None 0 0 0 0 0.0001 0.0001 0 0 0.0002 0 0.0001 0 – – – – – –
Nuclear Periphery 0.0002 0 0 0 0 0.0038 0 0 0.0004 0 0.0001 0 – – – – – –
Nucleolus 0.3666 0.1398 0.1478 0.0883 0.6012 0.2985 0.2164 0.098 0.1116 0.1442 0.6002 0.2648 – – – – – –
Nucleus 0.6239 0.8571 0.8497 0.9099 0.292 0.6868 0.78 0.9014 0.8776 0.8541 0.2965 0.7341 – – – – – –
Peroxisomes 0 0 0 0 0.0005 0 0 0 0 0 0.0005 0 – – – – – –
Punctate Nuclear 0.0002 0.0005 0 0 0 0.0003 0.0002 0 0.0001 0.0001 0 0 – – – – – –
Vacuole 0.0002 0.0001 0 0 0.0005 0.0006 0.0001 0 0.0007 0 0.0008 0.0001 – – – – – –
Vacuole Periphery 0.0006 0.0002 0.0001 0.0001 0.0016 0.0005 0.0002 0 0.0003 0.0001 0.0027 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 785.7577 682.4926 149.7137 118.657 847.168 462.2822 472.8474 266.0209 190.7672 675.3592
Translational Efficiency 2.4524 2.4373 1.8416 2.5569 2.3445 3.675 2.4106 2.8764 2.5807 2.8194

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1674 1219 215 914 1295 1806 1787 1521 2969 3025 2002 2435

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8218.35 9451.87 12561.21 11004.33 6678.89 9752.68 12025.06 11175.08 7546.88 9631.46 12082.64 11110.99
Standard Deviation 2888.15 2781.92 2224.62 3908.96 2883.58 2769.09 2420.19 3752.52 2985.42 2778.19 2405.68 3812.89
Intensity Change Log 2 — 0.201751 0.612055 0.421151 — 0.546190 0.848364 0.742605 — 0.366403 0.722808 0.574176

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002907 0.005747 0.007660 0.007230 0.001752 0.005374 0.016474 0.009715 0.002403 0.005524 0.015528 0.008783
Bud Neck 0.000988 0.001243 0.003397 0.004540 0.001084 0.001378 0.001893 0.003318 0.001030 0.001324 0.002055 0.003777
Bud Site 0.000231 0.000369 0.000165 0.001030 0.000295 0.000230 0.000124 0.001004 0.000259 0.000286 0.000128 0.001014
Cell Periphery 0.000121 0.000123 0.000058 0.000379 0.000140 0.000118 0.000171 0.000480 0.000129 0.000120 0.000159 0.000442
Cytoplasm 0.000601 0.000365 0.000017 0.000920 0.001011 0.000104 0.000033 0.000483 0.000780 0.000209 0.000031 0.000647
Cytoplasmic Foci 0.001525 0.002287 0.000229 0.004225 0.002950 0.002471 0.000482 0.003703 0.002147 0.002397 0.000455 0.003899
Eisosomes 0.006422 0.006841 0.000462 0.000550 0.002111 0.006385 0.006609 0.001161 0.004542 0.006568 0.005949 0.000932
Endoplasmic Reticulum 0.000273 0.000487 0.000805 0.001442 0.000142 0.000513 0.003043 0.002306 0.000216 0.000503 0.002802 0.001981
Endosome 0.000008 0.000003 0.000006 0.001350 0.000006 0.000038 0.000004 0.001174 0.000007 0.000023 0.000005 0.001240
Golgi 0.000012 0.000009 0.000043 0.001558 0.000020 0.000032 0.000011 0.001041 0.000016 0.000023 0.000015 0.001235
Lipid Particles 0.000012 0.000011 0.000007 0.000323 0.000005 0.000038 0.000010 0.001039 0.000009 0.000027 0.000009 0.000770
Mitochondria 0.001604 0.002053 0.010252 0.007894 0.001572 0.001968 0.003906 0.005942 0.001590 0.002002 0.004587 0.006675
Mitotic Spindle 0.000007 0.000004 0.000037 0.003423 0.000003 0.000233 0.000045 0.001363 0.000005 0.000141 0.000044 0.002136
None 0.001642 0.000962 0.000450 0.002141 0.004834 0.001069 0.000488 0.001727 0.003034 0.001026 0.000484 0.001883
Nuclear Periphery 0.000195 0.001021 0.027680 0.028793 0.000127 0.000714 0.015388 0.027034 0.000165 0.000838 0.016708 0.027694
Nuclear Periphery Foci 0.000014 0.000042 0.000404 0.000754 0.000011 0.000043 0.000170 0.000486 0.000013 0.000042 0.000195 0.000586
Nucleolus 0.175762 0.317148* 0.481844* 0.389431* 0.178882 0.308651* 0.388797* 0.299077* 0.177123 0.312075* 0.398790* 0.332993*
Nucleus 0.785303* 0.620940* 0.424587* 0.487771* 0.784232* 0.638076* 0.505977* 0.596123* 0.784835* 0.631171* 0.497237* 0.555452*
Peroxisomes 0.010839 0.016835 0.023090 0.012797 0.016845 0.017326 0.011291 0.007298 0.013458 0.017128 0.012558 0.009362
Vacuole 0.006569 0.011856 0.002943 0.015367 0.002002 0.006884 0.019518 0.014312 0.004577 0.008888 0.017738 0.014708
Vacuole Periphery 0.004965 0.011653 0.015863 0.028083 0.001978 0.008353 0.025565 0.021213 0.003662 0.009683 0.024523 0.023792

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.05 -5.90 -10.58 -2.60 0.60 -13.52* -23.18 -16.41 -8.00 8.68 -13.46 -22.73 -18.55 -8.14 10.37
Bud Neck -1.90 -7.90 -10.20 -9.06 -2.93 -3.50 -9.35 -10.33 -8.85 -6.54 -3.50 -10.98 -14.33 -12.61 -9.07
Bud Site -1.02 1.16 -2.88 -1.12 -3.15 1.09 3.06 -1.68 -1.85 -2.10 -0.79 3.25 -2.72 -2.11 -3.19
Cell Periphery -0.08 2.69 -2.45 -2.26 -3.06 0.51 -0.67 -2.02 -2.21 -1.89 0.30 -1.09 -2.84 -2.92 -2.61
Cytoplasm 0.12 2.05 -1.43 -1.27 -3.23 2.49 2.68 1.46 -3.92 -5.18 1.80 3.33 0.05 -2.16 -4.83
Cytoplasmic Foci -1.10 4.83 -2.34 -1.52 -3.30 0.56 3.48 -0.77 -1.18 -3.34 -0.45 3.78 -2.46 -1.88 -4.42
Eisosomes -0.43 8.87 8.77 9.12 -0.97 -7.91 -7.37 3.95 10.37 9.44 -3.60 -2.18 9.12 13.75 9.78
Endoplasmic Reticulum -4.30 -3.66 -6.58 -5.22 -2.78 -8.68 -12.19 -11.59 -9.38 2.50 -8.66 -12.11 -13.10 -10.72 3.32
Endosome 0.93 0.42 -2.48 -2.49 -2.49 -0.63 0.84 -2.92 -2.85 -2.95 -0.60 1.05 -3.84 -3.79 -3.86
Golgi 0.80 -4.35 -1.80 -1.81 -1.77 -0.18 1.53 -2.07 -2.06 -2.10 -0.28 0.85 -2.74 -2.73 -2.75
Lipid Particles 0.18 1.16 -1.15 -1.15 -1.17 -0.85 0.17 -1.80 -1.74 -1.80 -0.82 0.57 -2.04 -2.00 -2.05
Mitochondria -3.71 -8.40 -10.37 -9.56 2.01 -3.19 -16.20 -8.17 -7.38 -3.73 -4.69 -17.61 -12.57 -11.46 -4.78
Mitotic Spindle 0.75 -1.35 -2.55 -2.55 -2.52 -2.13 -1.07 -2.95 -2.42 -2.82 -2.10 -1.11 -3.68 -3.44 -3.59
None 2.68 5.12 -1.04 -2.48 -3.58 3.42 4.02 2.76 -1.57 -3.56 3.92 5.17 2.03 -2.74 -4.99
Nuclear Periphery -4.45 -5.27 -9.46 -9.17 -0.10 -2.40 -10.61 -11.84 -11.51 -4.27 -4.08 -11.79 -15.15 -14.72 -4.67
Nuclear Periphery Foci -3.68 -5.20 -5.48 -5.28 -2.42 -2.37 -8.96 -3.08 -2.86 -2.03 -3.43 -10.22 -5.34 -5.05 -3.63
Nucleolus -12.74 -14.13 -16.47 -4.60 4.20 -12.74 -19.97* -11.09 1.09 8.27 -18.26* -25.60* -19.01 -1.97 7.12
Nucleus 13.82 15.12 21.25 8.18 -2.67 13.33 24.88 15.84 3.38 -7.70 19.36 31.33 25.65 7.61 -5.90
Peroxisomes -4.08 -4.90 -1.65 2.66 4.08 -0.33 5.12 9.19 7.55 6.00 -3.40 1.12 5.66 7.76 4.71
Vacuole -3.10 3.88 -3.82 -1.28 -5.67 -5.47 -11.56 -8.73 -4.86 2.71 -4.75 -9.51 -8.04 -4.17 1.82
Vacuole Periphery -4.73 -4.07 -9.32 -5.89 -3.35 -6.22 -15.76 -13.34 -7.98 2.33 -7.20 -15.59 -15.56 -9.69 0.57
Standard name
Human Ortholog
Description Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
Localization
Cell Percentages nucleus (59%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Htb2

Htb2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Htb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available