Standard name
Human Ortholog
Description Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.22 0.4 0.41 0.38 0.41 0.44 0.38 0.29 – 0.35 0.26 0.28 0.48 0.42 0.37 0.33 0.42 0 0.09 0.15 0.35 0.36 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0 0.08 0 0 0 0 0 0
Endosome 0.15 0.08 0 0 0.22 0 0 0 0 – 0 0 0.09 0.13 0.14 0.13 0.21 0.17 0.11 0.07 0.05 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.22 0.36 0.57 0.31 0.53 0.54 0.63 0.8 – 0.69 0.81 0.24 0.17 0.22 0.12 0 0.06 0.06 0.07 0.2 0.06 0.1 0.11
Nucleus 0.5 0.2 0.13 0.09 0.05 0 0.05 0.08 0 – 0 0.08 0.21 0.14 0.08 0.22 0.23 0.19 0.24 0.28 0.12 0.11 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0.09 0.07 0.1 0.07 0 0.05 0 – 0 0.05 0 0 0 0 0.07 0.05 0.16 0.13 0.12 0.13 0.11 0.11
Vac/Vac Membrane 0.21 0.38 0.07 0 0.09 0.05 0 0 0 – 0.08 0 0.24 0.16 0.21 0.23 0.27 0.19 0.18 0.21 0.23 0.22 0.21 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 2 2 0 0 4 3
Bud 1 0 2 5 0 3 7 13 8 – 8 12 0 2 12 1 0 0 3 3 1 2 10 9
Bud Neck 1 0 1 0 1 1 1 5 0 – 0 2 0 0 0 1 0 1 3 3 2 1 1 4
Bud Site 0 0 0 1 1 5 2 9 7 – 2 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 20 44 40 50 67 82 120 132 73 – 46 50 53 155 145 109 60 73 8 27 26 65 107 147
Endoplasmic Reticulum 2 2 2 0 0 1 0 0 0 – 0 0 0 1 0 23 7 14 1 1 2 0 1 5
Endosome 22 16 2 0 39 9 5 6 0 – 1 2 17 43 47 37 37 29 26 20 8 9 12 11
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 1 2 0 0 0 0
Mitochondria 10 44 36 69 54 106 148 216 202 – 90 157 46 53 76 34 6 11 14 21 35 11 30 36
Nucleus 74 40 13 11 9 8 14 26 11 – 4 15 40 44 29 65 42 34 57 83 20 20 15 9
Nuclear Periphery 1 2 0 0 0 0 1 0 0 – 0 0 0 1 2 0 0 1 4 1 0 0 0 1
Nucleolus 0 1 0 0 0 0 1 1 1 – 0 1 1 1 1 0 0 1 19 12 3 5 3 2
Peroxisomes 0 0 0 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 9 4 9 9 18 13 11 16 8 – 1 10 4 11 3 13 13 9 39 39 21 23 32 35
Vac/Vac Membrane 31 76 7 2 15 9 7 5 6 – 10 6 45 52 73 66 49 33 43 63 41 40 62 55
Unique Cell Count 149 200 100 122 175 200 275 344 254 130 195 188 320 344 292 180 175 239 301 178 188 296 338
Labelled Cell Count 172 231 113 147 206 237 317 429 316 162 263 206 363 388 351 215 207 239 301 178 188 296 338


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.0 3.5 3.3 3.6 3.2 3.1 3.1 2.7 – 3.0 2.5 3.9 4.0 3.9 5.2 5.6 5.7 4.2 4.5 4.9
Std Deviation (1e-4) 0.8 1.0 0.6 1.3 1.6 0.9 1.3 0.9 1.4 – 2.0 1.0 0.7 1.1 1.3 1.1 1.4 1.7 1.4 2.3 1.8
Intensity Change (Log2) – – – -0.08 0.07 -0.1 -0.16 -0.18 -0.35 – -0.19 -0.47 0.17 0.2 0.16 0.57 0.68 0.72 0.29 0.37 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.5 4.1 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9488 0.7253 0.9902 1.0528 0.4324 0.8058 1.5655 1.4663 1.4146 1.3947 0.3952 1.5382 1.8065 1.6724 1.5765 2.396 1.3976 2.2063
Actin 0.059 0.0006 0.0215 0.0206 0.0138 0.0043 0.0221 0.0008 0.0067 0.0045 0.0004 0.0011 0.02 0.0006 0.0006 0.035 0.0016 0.0047
Bud 0.0012 0.0004 0.0003 0.0004 0.0008 0.0002 0.0008 0.0024 0.0009 0.0003 0.0002 0.0006 0.0003 0.0005 0.0008 0.0002 0.0001 0.0004
Bud Neck 0.0082 0.0024 0.0042 0.0024 0.0022 0.0066 0.0179 0.0013 0.0026 0.0011 0.0031 0.0068 0.0027 0.0016 0.0009 0.0017 0.0016 0.0069
Bud Periphery 0.0019 0.0012 0.0005 0.0008 0.0016 0.0004 0.0011 0.0027 0.0003 0.0003 0.0002 0.0017 0.0004 0.0005 0.0014 0.0002 0.0001 0.0006
Bud Site 0.0066 0.0081 0.0019 0.0019 0.0021 0.0004 0.0062 0.0113 0.0022 0.0032 0.0003 0.0009 0.0028 0.0201 0.0011 0.0008 0.0002 0.0004
Cell Periphery 0.0005 0.0008 0.0004 0.0003 0.0003 0.0001 0.0003 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.1157 0.2168 0.1557 0.1576 0.1752 0.1286 0.1081 0.1832 0.1199 0.1028 0.1448 0.127 0.1054 0.1375 0.1055 0.0588 0.0908 0.0989
Cytoplasmic Foci 0.018 0.0058 0.0083 0.0181 0.0203 0.0054 0.0291 0.0059 0.011 0.0099 0.0074 0.0036 0.0155 0.0085 0.012 0.0148 0.0052 0.0106
Eisosomes 0.0007 0.0001 0.0007 0.0002 0.0002 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0151 0.0067 0.0059 0.0061 0.0102 0.0035 0.0082 0.0054 0.0034 0.0042 0.0039 0.0026 0.003 0.0058 0.0031 0.0025 0.0026 0.0028
Endosome 0.0464 0.0045 0.0092 0.0256 0.0238 0.0105 0.0361 0.0088 0.0111 0.0137 0.0149 0.0084 0.0115 0.0106 0.0067 0.0048 0.0022 0.0046
Golgi 0.0115 0.0002 0.0034 0.0117 0.0078 0.0018 0.0068 0.0005 0.0015 0.0017 0.0004 0.0022 0.0014 0.0002 0.0003 0.0024 0.0001 0.0006
Lipid Particles 0.0189 0.0008 0.0044 0.0196 0.0086 0.0023 0.0262 0.0007 0.0041 0.0018 0.0007 0.0007 0.0026 0.0016 0.0008 0.004 0.0004 0.0018
Mitochondria 0.008 0.0009 0.0063 0.0411 0.0217 0.0066 0.0057 0.0029 0.0016 0.0033 0.0008 0.002 0.0014 0.0008 0.0013 0.049 0.0013 0.0008
None 0.1489 0.2741 0.2358 0.0305 0.0808 0.1187 0.2087 0.2166 0.2326 0.1988 0.1667 0.1589 0.2104 0.2726 0.2533 0.1521 0.3231 0.2192
Nuclear Periphery 0.0696 0.0142 0.0197 0.0088 0.0199 0.0211 0.0431 0.0161 0.019 0.0216 0.0201 0.0118 0.0431 0.0293 0.0289 0.0394 0.0503 0.0218
Nucleolus 0.008 0.0064 0.0104 0.0076 0.008 0.0146 0.0089 0.008 0.0089 0.0068 0.0116 0.0125 0.0135 0.0099 0.0077 0.0095 0.0083 0.0137
Nucleus 0.3269 0.3943 0.4351 0.5281 0.5159 0.5454 0.3286 0.4495 0.4629 0.5373 0.504 0.5627 0.4419 0.374 0.4584 0.5177 0.3525 0.5203
Peroxisomes 0.0156 0.0002 0.0021 0.0113 0.0017 0.0007 0.0221 0.0005 0.0022 0.0022 0.0004 0.0006 0.0287 0.0003 0.0005 0.0077 0.0004 0.0008
Punctate Nuclear 0.1087 0.0568 0.0699 0.092 0.0774 0.1258 0.1112 0.0752 0.1049 0.0831 0.1174 0.0926 0.0909 0.1193 0.1137 0.0978 0.158 0.0891
Vacuole 0.0069 0.0042 0.0035 0.0079 0.005 0.0023 0.006 0.0065 0.003 0.0022 0.002 0.0027 0.0027 0.0051 0.0021 0.0011 0.0006 0.0015
Vacuole Periphery 0.0037 0.0004 0.0008 0.0075 0.0026 0.0009 0.0027 0.0014 0.0009 0.001 0.0006 0.0006 0.0015 0.0009 0.0006 0.0006 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0939 6.0576 8.4905 10.4509 6.5893 10.535 21.9473 22.2768 14.6852 17.3125
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate
Localization
Cell Percentages nucleus (39%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pdr3

Pdr3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdr3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available