Standard name
Human Ortholog
Description Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.74 0.59 0.73 0.64 0.7 0.52 0.49 0.38 0.41 0.39 0.42 0.4 0.73 0.73 0.74 0.37 0.32 0.35 0.88 0.8 0.85 0.86 0.88 0.88
Bud 0.22 0.19 0.2 0.23 0.37 0.21 0.21 0.18 0.23 0.16 0.18 0.23 0.19 0.18 0.19 0.06 0 0.05 0 0.08 0.06 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.09 0.09 0.11 0.1 0.05 0 0.05 0.05 0 0 0.08 0.06 0.09 0.13 0.15 0.05 0.21 0.16 0 0 0 0 0 0
Cytoplasm 0 0.11 0.06 0.07 0.08 0.05 0.06 0.07 0.05 0.05 0 0 0.05 0.06 0 0.25 0.21 0.44 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.09 0.07 0.05 0.06 0.05 0.05 0 0 0 0.06 0 0.06 0.08 0.08 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.4 0.09 0.12 0.08 0.1 0.06 0.09 0.1 0.05 0.06 0.05 0.06 0.28 0.2 0.24 0.44 0.37 0.37 0 0 0 0 0 0
Mitochondria 0.05 0.36 0.35 0.57 0.29 0.67 0.58 0.64 0.78 0.78 0.74 0.65 0.21 0.23 0.46 0.16 0.05 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.12 0.15 0.11 0.15 0.11 0.08 0.13 0.11 0.14 0.12 0.17 0.18 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 181 38 178 141 178 169 202 170 126 144 148 162 143 174 188 51 6 22 291 83 254 126 266 254
Bud 54 12 49 50 95 67 86 81 69 59 63 94 37 42 48 8 0 3 6 8 17 7 9 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3 2 2
Bud Site 5 0 11 6 3 12 11 11 7 14 7 6 4 3 0 1 0 0
Cell Periphery 22 6 26 21 12 7 22 23 12 14 28 26 17 30 39 7 4 10 2 0 1 0 0 0
Cytoplasm 10 7 14 16 20 16 23 30 15 17 10 10 9 14 5 34 4 28 1 1 4 0 0 1
Endoplasmic Reticulum 13 6 16 10 15 15 22 14 12 14 21 6 11 18 19 4 0 3 2 1 1 1 0 0
Endosome 3 0 3 3 4 9 5 2 1 1 1 9 2 0 1 5 0 0 3 1 5 1 8 2
Golgi 99 6 29 18 26 21 38 43 15 21 18 24 56 48 61 60 7 23 8 1 3 1 6 8
Mitochondria 12 23 85 125 73 219 239 289 239 289 261 262 41 55 117 22 1 8 3 3 2 0 1 1
Nucleus 0 0 1 0 1 1 1 3 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nuclear Periphery 0 1 0 0 2 1 2 5 2 2 4 6 1 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 3 1 0 3 6 6 13 13 7 10 7 13 1 0 1 2 1 1 0 0 0 0 0 0
Peroxisomes 3 1 18 26 37 37 61 50 25 47 37 55 24 41 46 3 0 0 2 0 5 1 2 10
SpindlePole 6 0 2 2 2 1 6 15 1 3 5 8 5 1 0 6 1 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 4 3 1 1 2 4 0 1 0 11 1 0 0 4 1 2 0 0 1 0 2 1
Unique Cell Count 246 64 243 221 255 326 411 450 305 369 351 404 197 239 253 137 19 63 329 105 300 146 303 291
Labelled Cell Count 413 101 436 424 475 582 733 753 531 637 610 692 352 426 525 207 25 100 329 105 300 146 303 291


Actin, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.5 18.4 15.7 14.5 15.4 14.6 13.9 13.0 12.9 12.1 12.5 12.0 19.6 18.7 16.2 23.1 26.1 25.6 20.0 19.4 21.1
Std Deviation (1e-4) 5.8 6.2 4.5 3.9 5.0 4.3 4.0 3.8 4.0 3.6 3.8 3.3 4.8 4.9 4.2 11.3 12.6 13.2 5.2 4.1 4.5
Intensity Change (Log2) -0.11 -0.03 -0.1 -0.18 -0.27 -0.28 -0.37 -0.32 -0.39 0.32 0.26 0.04 0.56 0.74 0.71 0.35 0.31 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.2 -0.9 -5.2 -6.0 -8.9 -7.5 -8.3 -7.5 -8.2 -0.2 -0.1 0.3 -6.9 -3.8 -5.7
Bud 0.6 4.2 0.1 0.2 -0.7 0.7 -1.3 -0.7 0.9 -0.4 -0.7 -0.3 -3.8 0 -2.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.0 -2.3 -0.5 -1.3 -1.5 -1.5 -0.4 -1.8 -2.3 -1.4 -2.1 -3.4 0 0 0
Cell Periphery -0.4 -2.5 -4.3 -2.5 -2.7 -3.1 -3.4 -1.1 -1.9 -0.7 0.6 1.6 -1.9 0 1.1
Cytoplasm 0.6 0.9 -0.5 -0.1 0.5 -0.4 -0.6 -1.8 -2.1 -0.6 0 -2.2 5.4 0 8.0
Endoplasmic Reticulum -1.0 -0.3 -1.0 -0.7 -2.1 -1.4 -1.6 -0.3 -3.5 -0.4 0.4 0.4 -1.5 0 0
Endosome 0 0 1.3 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.4 -0.6 -2.3 -1.1 -1.0 -3.0 -2.8 -3.0 -2.7 4.4 2.4 3.5 7.0 0 4.6
Mitochondria 4.7 -1.5 7.6 5.7 7.4 10.3 10.8 9.6 7.4 -3.3 -2.9 2.6 -3.9 -2.7 -3.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.6 2.5 1.6 2.8 1.6 0.3 2.1 1.3 2.4 1.7 3.3 3.6 -2.1 0 0
SpindlePole 0 0 0 0 2.0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.3715 25.1085 23.7211 23.665 22.3941 24.5012 18.6002 20.8727 19.3974 17.5227 20.3158 20.7814 24.7392 17.1832 17.02 19.7055 19.7183 19.4061
Actin 0.9519 0.8879 0.937 0.8049 0.9453 0.9022 0.8845 0.8488 0.8839 0.8743 0.9401 0.9444 0.9886 0.9898 0.9161 0.9899 0.9926 0.9794
Bud 0.0002 0.0009 0.0014 0.0028 0.002 0.0004 0.0008 0.004 0.0033 0.005 0.0004 0.0007 0.0001 0.0003 0.0077 0.0016 0.0004 0.0001
Bud Neck 0.0029 0.004 0.0023 0.0059 0.0026 0.02 0.0043 0.0087 0.0037 0.0041 0.0009 0.0111 0.0012 0.0012 0.0014 0.0013 0.0053 0.0041
Bud Periphery 0.0002 0.0007 0.0015 0.0037 0.0052 0.0008 0.0009 0.0022 0.0037 0.0083 0.0005 0.0025 0.0001 0.0002 0.0126 0.0025 0.0006 0.0001
Bud Site 0.0011 0.0347 0.0075 0.012 0.0029 0.0004 0.0167 0.1269 0.0223 0.0085 0.0007 0.0013 0.0081 0.0035 0.0049 0.001 0.0002 0.0002
Cell Periphery 0.0001 0.0008 0.0003 0.0023 0.0007 0.001 0.0008 0.0008 0.0006 0.0008 0 0.0003 0.0001 0 0.0003 0.0002 0 0
Cytoplasm 0 0.0257 0.0352 0.1308 0.0001 0.0126 0.0031 0.0035 0.0022 0.0026 0.0206 0.0003 0 0 0.0002 0.0001 0 0
Cytoplasmic Foci 0.0004 0.0044 0.0008 0.0017 0.0006 0.0003 0.0199 0.0009 0.0118 0.045 0.0054 0.005 0.0002 0.0011 0.0281 0.0002 0 0.0001
Eisosomes 0.0376 0.0255 0.0035 0.0133 0.0048 0.0503 0.0033 0.0017 0.0009 0.0007 0.0004 0.0042 0.0011 0.0005 0.0002 0.0003 0.0005 0.0154
Endoplasmic Reticulum 0 0.0001 0.0001 0.0108 0.0001 0.0001 0.0025 0.0001 0.0037 0.0007 0.0044 0.0005 0 0 0 0 0 0
Endosome 0.0001 0.0008 0.0002 0.0013 0.0005 0.0002 0.0148 0.0001 0.0249 0.008 0.0139 0.0056 0 0 0.0013 0.0001 0 0
Golgi 0.0045 0.0044 0.0097 0.0047 0.0336 0.0106 0.0127 0.001 0.0128 0.018 0.0013 0.007 0.0002 0.0007 0.0021 0.0025 0.0002 0.0004
Lipid Particles 0.0002 0.0067 0.0002 0.0018 0.0006 0.0003 0.0072 0.0001 0.0059 0.0061 0.0007 0.0058 0 0.0001 0.0077 0.0001 0 0
Mitochondria 0.0001 0.0007 0.0001 0.0004 0.0005 0.0005 0.0053 0.0001 0.0034 0.0022 0.0005 0.0067 0 0.0002 0.0003 0.0001 0 0
None 0 0.0001 0 0.0001 0 0 0.0037 0 0.0008 0.0012 0.0047 0.0004 0 0 0.0001 0 0 0
Nuclear Periphery 0 0 0 0.0004 0 0 0.0064 0 0.0034 0.0003 0.0021 0.0004 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.0005 0 0.0002 0.0001 0.0001 0.0001 0 0 0 0 0 0
Nucleus 0 0 0 0.0002 0 0 0.0008 0.0001 0.0005 0.0002 0.0009 0.0001 0 0 0 0 0 0
Peroxisomes 0.0006 0.002 0.0002 0.0002 0.0003 0.0003 0.0068 0.0005 0.0087 0.0116 0.0003 0.002 0.0003 0.0024 0.0162 0.0001 0.0001 0.0002
Punctate Nuclear 0 0.0001 0 0.0001 0 0 0.0022 0.0001 0.0006 0.0013 0.0011 0.0011 0 0 0.0005 0 0 0
Vacuole 0 0.0003 0 0.0023 0 0 0.0017 0.0002 0.0019 0.0007 0.0007 0.0002 0 0 0 0 0 0
Vacuole Periphery 0 0 0 0.0002 0 0 0.001 0 0.0009 0.0003 0.0003 0.0002 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 106.6477 87.6779 97.9831 107.0404 93.076 101.8645 102.7364 112.6383 117.7337 104.7286
Translational Efficiency 1.1509 1.1521 1.1551 1.1822 1.1354 1.2 0.9203 0.9568 0.9663 0.9874

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
Localization
Cell Percentages actin (99%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sla1

Sla1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sla1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available