Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0.05 0.09 0.08 0.05 0.08 0 0.07 0.08
Bud 0 0.06 0 0.06 0.06 0.05 0.07 0.07 0.09 0.1 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.68 0.66 0.51 0.31 0.21 0.24 0.31 0.17 0.23 0.18 0.25 0.65 0.59 0.56 0.59 0.48 0.48 0.23 0.36 0.24 0.45 0.33 0.26
Endoplasmic Reticulum 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0.1 0 0.11 0.19 0.23 0.22 0.21 0.17 0 0.05 0 0.05 0.08 0.22 0.29 0.22 0.31 0.22 0.22 0.07 0.13 0.1 0.13 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.28 0 0.18 0.17 0.43 0.37 0.25 0.76 0.66 0.74 0.57 0.05 0 0 0.09 0.1 0.09 0.25 0.31 0.33 0.25 0.25 0.26
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0.12 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.15 0.1 0.19 0.18 0.2 0.22 0.1 0.14 0.11 0.15 0.07 0.1 0.12 0.12 0.1 0.14 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0 0 0.07 0.05 0.11 0.06 0.07 0.12 0 0 0 0 0 0 0 0.06 0.06 0.09 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 8 4 10 6 3 3 2 5 2 10 5 15 19 12 1 13 26 15 10 25 10 24 30
Bud 5 8 6 15 22 21 41 28 38 44 37 51 5 12 3 6 10 9 2 1 11 4 7 8
Bud Neck 0 0 1 2 7 1 5 2 1 1 5 1 1 0 1 0 0 0 5 7 8 4 11 9
Bud Site 0 1 0 0 0 9 6 0 8 7 6 8 0 0 0 1 0 1 – – – – – –
Cell Periphery 1 1 2 4 3 3 3 4 1 0 0 3 1 0 2 4 4 1 0 0 1 0 0 0
Cytoplasm 78 96 154 126 110 94 141 128 76 103 69 120 97 200 202 127 136 133 45 66 81 120 117 99
Endoplasmic Reticulum 14 8 1 0 4 5 0 1 5 0 3 3 16 11 14 6 14 13 6 0 6 2 4 6
Endosome 13 1 26 47 82 102 124 71 13 21 9 26 12 74 104 47 87 60 44 13 45 26 45 62
Golgi 1 0 0 1 0 0 1 1 0 0 0 0 1 0 2 0 2 6 7 3 5 4 6 12
Mitochondria 18 40 10 45 62 194 215 105 339 289 285 270 7 12 1 19 27 26 50 57 115 66 88 101
Nucleus 1 0 0 0 1 3 3 11 2 5 1 6 0 1 1 1 2 8 0 1 2 1 4 2
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 0 1 0 2 3 4 2 5 2 4 7 0 0 0 0 2 1 0 0 0 1 1 0
Peroxisomes 0 0 0 14 44 30 24 22 11 9 9 21 0 12 12 3 5 1 2 1 6 0 3 4
SpindlePole 3 1 35 26 69 81 117 91 43 60 42 73 10 33 45 27 28 38 7 5 14 7 22 24
Vac/Vac Membrane 4 1 16 12 40 26 40 49 12 8 8 13 0 5 11 12 16 25 3 11 4 11 9 11
Unique Cell Count 132 141 234 249 356 454 579 414 447 440 384 477 149 337 362 217 281 276 197 185 344 271 360 385
Labelled Cell Count 146 165 256 302 452 575 727 518 559 552 488 608 166 379 410 254 346 348 197 185 344 271 360 385


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.2 6.6 5.9 6.4 5.1 5.2 5.8 4.3 4.6 4.2 4.7 5.3 5.7 5.8 5.5 6.5 6.7 6.9 6.9 6.9
Std Deviation (1e-4) 0.7 0.6 2.2 1.6 2.1 1.2 1.7 1.7 0.9 0.9 0.9 1.1 0.9 0.9 1.3 1.2 1.3 1.2 2.0 2.0 1.9
Intensity Change (Log2) – – – -0.16 -0.05 -0.38 -0.34 -0.18 -0.62 -0.53 -0.63 -0.49 -0.31 -0.2 -0.18 -0.26 -0.01 0.02 0.06 0.06 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.6 -0.5 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0702 4.3827 4.295 4.0404 4.0332 4.4068 2.7209 3.5885 3.7755 2.8711 3.2625 4.0488 0.2962 1.1055 0.9485 0.8978 1.0634 0.8107
Actin 0.0427 0.0068 0.0359 0.0305 0.0497 0.011 0.013 0.01 0.0188 0.0025 0.0359 0.0122 0.0279 0.0016 0.02 0.0018 0.0222 0.0035
Bud 0.0021 0.0031 0.0081 0.0019 0.0044 0.0007 0.0002 0.0003 0.0008 0.0003 0.001 0.0003 0.0014 0.0007 0.0035 0.0005 0.0022 0.0004
Bud Neck 0.0032 0.0012 0.0042 0.001 0.0031 0.0366 0.0008 0.0007 0.0157 0.0002 0.0046 0.0092 0.0102 0.0007 0.0091 0.0035 0.0011 0.0319
Bud Periphery 0.0035 0.0025 0.0143 0.0025 0.0062 0.001 0.0003 0.0003 0.0008 0.0005 0.0012 0.001 0.0014 0.0005 0.0045 0.0003 0.0022 0.0004
Bud Site 0.0151 0.0586 0.0204 0.0014 0.003 0.0015 0.001 0.0057 0.0113 0.0004 0.0018 0.0003 0.0083 0.008 0.0055 0.002 0.0046 0.0008
Cell Periphery 0.0011 0.0022 0.0008 0.0004 0.0005 0.0005 0.0003 0.0014 0.0006 0.0001 0.0002 0.0003 0.0005 0.0004 0.0003 0.0001 0.0002 0.0002
Cytoplasm 0.2584 0.3544 0.2973 0.318 0.3505 0.3502 0.2357 0.2959 0.3439 0.2086 0.2329 0.2653 0.278 0.3781 0.3693 0.3451 0.3357 0.4094
Cytoplasmic Foci 0.2034 0.177 0.1961 0.2008 0.1642 0.1943 0.2032 0.0823 0.1258 0.106 0.3082 0.1682 0.2141 0.2044 0.1855 0.2067 0.1835 0.188
Eisosomes 0.0005 0.0002 0.0004 0.0002 0.0004 0.0001 0.0007 0.0006 0.0004 0.0001 0.0002 0.0001 0.0004 0.0001 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0084 0.0071 0.003 0.002 0.0025 0.0022 0.0028 0.0045 0.0054 0.0017 0.0041 0.0041 0.0081 0.0058 0.0052 0.0026 0.0034 0.0027
Endosome 0.1212 0.1331 0.1149 0.153 0.1101 0.1034 0.1071 0.0431 0.0959 0.1675 0.0774 0.0939 0.1788 0.1359 0.1357 0.187 0.1704 0.105
Golgi 0.0397 0.0231 0.0328 0.0268 0.0283 0.0287 0.0155 0.0043 0.0313 0.0116 0.019 0.0173 0.0327 0.0194 0.0381 0.0284 0.0274 0.0228
Lipid Particles 0.0116 0.0102 0.0085 0.0043 0.0117 0.0018 0.0079 0.0017 0.0076 0.0048 0.0088 0.005 0.0127 0.0086 0.0061 0.0076 0.0268 0.0045
Mitochondria 0.0217 0.0058 0.0128 0.0176 0.006 0.0055 0.0057 0.0004 0.0062 0.0018 0.0101 0.0078 0.0082 0.0064 0.0063 0.0059 0.0083 0.0141
None 0.2039 0.176 0.2013 0.1913 0.1785 0.2209 0.3392 0.5424 0.2875 0.4451 0.2625 0.3731 0.1679 0.2036 0.1591 0.123 0.1548 0.1909
Nuclear Periphery 0.0078 0.0022 0.0012 0.0005 0.0018 0.0004 0.0213 0.0007 0.0013 0.0005 0.0008 0.0006 0.0055 0.001 0.0012 0.0018 0.0019 0.0017
Nucleolus 0.0006 0.0006 0.0006 0.0002 0.0005 0.0003 0.0018 0.0002 0.0011 0.0001 0.0001 0.0001 0.0005 0.0003 0.0003 0.0003 0.0005 0.0002
Nucleus 0.0085 0.0017 0.0031 0.0007 0.0014 0.0012 0.0109 0.0006 0.0014 0.0003 0.0005 0.0003 0.0029 0.0012 0.0011 0.0036 0.0013 0.0011
Peroxisomes 0.0361 0.0254 0.0366 0.0374 0.0682 0.0308 0.018 0.0017 0.025 0.0434 0.0258 0.0376 0.0271 0.0146 0.0323 0.0579 0.0367 0.0153
Punctate Nuclear 0.0045 0.0017 0.0017 0.0012 0.0041 0.0061 0.0076 0.0007 0.0048 0.0012 0.0032 0.0012 0.0029 0.0013 0.0102 0.0155 0.0105 0.0023
Vacuole 0.0044 0.0061 0.0046 0.0049 0.0038 0.002 0.005 0.0025 0.0132 0.0029 0.0013 0.0014 0.0085 0.0065 0.0052 0.005 0.0048 0.0037
Vacuole Periphery 0.0015 0.0011 0.0013 0.0033 0.0012 0.0007 0.002 0.0002 0.0013 0.0007 0.0004 0.0005 0.002 0.0012 0.0013 0.0016 0.0016 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7435 3.5102 6.0777 10.5246 13.5764 14.39 5.9153 7.1864 8.9613 13.9871
Translational Efficiency 2.0109 4.1955 2.0612 1.6638 1.692 2.1787 2.0624 1.638 1.4365 1.6492

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1395 1066 1337 365 1595 2133 179 1424 2990 3199 1516 1789

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 698.38 686.90 899.22 908.07 653.70 813.47 925.17 918.32 674.55 771.29 902.28 916.23
Standard Deviation 73.75 89.82 112.72 121.43 75.33 95.25 102.91 114.59 77.86 110.89 111.92 116.09
Intensity Change Log 2 — -0.023912 0.364662 0.378791 — 0.315460 0.501090 0.490368 — 0.150138 0.432234 0.433814

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001796 0.001001 0.017757 0.009282 0.001730 0.002099 0.009275 0.005776 0.001761 0.001733 0.016755 0.006491
Bud Neck 0.025346 0.014569 0.012683 0.010521 0.018486 0.023903 0.013799 0.010363 0.021687 0.020792 0.012815 0.010395
Bud Site 0.013249 0.023083 0.055020 0.055280 0.015947 0.021849 0.059680 0.038856 0.014688 0.022260 0.055570 0.042207
Cell Periphery 0.000260 0.000128 0.000161 0.000113 0.000140 0.000137 0.000118 0.000067 0.000196 0.000134 0.000156 0.000077
Cytoplasm 0.173377* 0.174586* 0.146610* 0.156036* 0.116557* 0.157185* 0.092677 0.159381* 0.143067* 0.162983* 0.140242* 0.158699*
Cytoplasmic Foci 0.418879* 0.428184* 0.259758* 0.246265* 0.433725* 0.449852* 0.327021* 0.297898* 0.426799* 0.442632* 0.267700* 0.287364*
Eisosomes 0.000667 0.000177 0.000118 0.000109 0.000488 0.000453 0.000094 0.000074 0.000571 0.000361 0.000115 0.000081
Endoplasmic Reticulum 0.003592 0.002068 0.004618 0.000998 0.002047 0.000751 0.001761 0.000678 0.002768 0.001190 0.004281 0.000744
Endosome 0.080955 0.120026 0.064814 0.128148 0.103999 0.088426 0.107658 0.140210 0.093248 0.098956 0.069873 0.137749
Golgi 0.072288 0.065593 0.021460 0.015455 0.107982 0.097229 0.015993 0.030482 0.091329 0.086687 0.020815 0.027416
Lipid Particles 0.042855 0.021357 0.013249 0.007125 0.038166 0.018018 0.009830 0.006574 0.040353 0.019131 0.012846 0.006687
Mitochondria 0.044279 0.028262 0.002834 0.001605 0.061490 0.046854 0.001721 0.003023 0.053460 0.040659 0.002702 0.002733
Mitotic Spindle 0.002498 0.008093 0.170427* 0.062293 0.004176 0.005537 0.092411 0.059026 0.003393 0.006389 0.161215* 0.059692
None 0.000942 0.002058 0.000589 0.000547 0.001389 0.000994 0.000147 0.000778 0.001180 0.001349 0.000537 0.000731
Nuclear Periphery 0.000445 0.000375 0.000925 0.000336 0.000465 0.000121 0.000303 0.000337 0.000456 0.000206 0.000851 0.000337
Nuclear Periphery Foci 0.000336 0.001215 0.006968 0.002931 0.000758 0.000211 0.004799 0.001288 0.000561 0.000546 0.006712 0.001623
Nucleolus 0.000909 0.002253 0.001035 0.001019 0.001443 0.000419 0.000567 0.000504 0.001194 0.001030 0.000980 0.000609
Nucleus 0.021833 0.012482 0.028650 0.009371 0.011425 0.007780 0.006838 0.007935 0.016281 0.009347 0.026074 0.008228
Peroxisomes 0.033055 0.022946 0.104993* 0.094898 0.044284 0.038731 0.130787* 0.079852 0.039045 0.033471 0.108039* 0.082922
Vacuole 0.054059 0.058934 0.086220 0.196130 0.028962 0.033653 0.123403 0.154430 0.040671 0.042078 0.090610 0.162938
Vacuole Periphery 0.008382 0.012612 0.001111 0.001539 0.006340 0.005796 0.001121 0.002467 0.007293 0.008067 0.001112 0.002278

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.53 -9.65 -2.60 -3.15 4.26 -1.25 -3.78 -4.29 -3.67 2.07 0.08 -10.54 -4.81 -5.07 7.32
Bud Neck 5.69 6.59 7.77 3.13 2.69 -3.03 1.92 5.58 8.90 1.01 0.62 6.60 9.56 9.24 3.28
Bud Site -4.95 -14.41 -5.36 -3.43 1.66 -3.36 -5.41 -7.52 -5.13 2.84 -5.86 -15.05 -9.88 -6.14 5.28
Cell Periphery 6.59 4.75 8.10 2.22 6.08 0.26 0.96 9.00 8.90 4.07 5.52 2.86 12.72 9.48 12.45
Cytoplasm -0.19 1.01 -0.99 -0.83 -1.54 -6.72 0.56 -9.28 -4.09 -5.54 -4.02 -2.37 -6.76 -3.72 -3.69
Cytoplasmic Foci -1.00 23.66 19.41 19.35 3.62 -2.36 8.01 23.94 27.08 3.28 -2.92 28.99 29.86 32.32 0.28
Eisosomes 7.96 10.08 10.29 2.55 1.73 0.54 6.57 7.15 13.97 3.45 4.64 11.24 12.31 13.59 7.13
Endoplasmic Reticulum 2.67 -3.33 4.62 2.98 10.74 4.84 0.22 5.23 0.42 3.52 5.40 -5.63 7.13 3.15 12.47
Endosome -7.49 6.22 -0.81 3.46 -3.64 3.67 0.74 -1.25 -4.49 -1.38 -1.75 9.57 -2.93 -1.65 -10.00
Golgi 1.53 15.77 15.94 13.80 3.07 2.21 22.01 21.00 22.46 -3.21 1.37 26.77 24.91 25.29 -1.43
Lipid Particles 6.90 10.62 12.60 7.85 5.40 8.62 9.41 14.75 9.65 1.67 11.21 15.19 19.43 12.50 7.85
Mitochondria 4.78 16.27 16.85 11.84 3.42 3.97 19.22 19.67 20.74 -0.88 5.00 25.31 25.55 23.60 1.16
Mitotic Spindle -3.83 -22.23* -6.32 -5.64 8.81 -1.50 -5.89 -13.14 -12.73 1.71 -3.90 -22.83 -14.71 -13.81 11.85
None -2.35 0.78 0.95 3.71 0.46 0.76 2.99 1.17 0.33 -1.22 -0.41 2.20 1.10 1.34 -0.38
Nuclear Periphery 0.61 -7.45 -2.26 -2.83 5.77 2.87 -0.01 -0.67 -8.35 -0.76 3.29 -6.95 -1.50 -7.79 6.76
Nuclear Periphery Foci -4.39 -8.51 -5.64 -4.21 3.89 3.87 -5.18 -3.72 -6.96 4.27 0.13 -8.94 -7.09 -7.22 6.96
Nucleolus -2.58 -1.21 -1.00 1.93 0.13 4.11 2.25 2.80 -2.81 0.29 0.84 0.09 2.10 1.07 1.81
Nucleus 4.56 -5.85 3.09 -0.98 7.97 3.22 1.11 -0.70 -4.52 -1.68 6.50 -7.89 2.76 -3.66 9.40
Peroxisomes 4.11 -12.32 -3.58 -4.96 3.42 2.03 -5.09 -5.13 -6.96 3.55 2.89 -12.53 -7.30 -9.21 5.67
Vacuole -1.38 -12.46 -16.16 -15.80 -11.67 -2.20 -8.16 -27.63 -27.06 -3.29 -0.78 -17.82 -30.53 -30.46 -14.64
Vacuole Periphery -3.51 11.84 11.27 11.20 -0.21 0.97 11.44 8.56 7.62 -2.03 -1.34 17.14 12.41 12.51 -2.44
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
Localization
Cell Percentages mitochondrion (18%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Laa2

Laa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Laa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available