Standard name | |
---|---|
Human Ortholog | |
Description | Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 1 | 4 | 5 | 16 | 23 | 42 |
Bud | 1 | 17 | 9 | 16 | 17 | 14 | 21 | 27 | 32 | 28 | 2 | 2 | 9 | 18 | 14 | 2 | – | – | 0 | 1 | 2 | 15 | 13 | 25 |
Bud Neck | 1 | 0 | 3 | 0 | 3 | 2 | 1 | 1 | 2 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | – | – | 1 | 2 | 1 | 6 | 7 | 23 |
Bud Site | 1 | 1 | 1 | 0 | 1 | 0 | 14 | 6 | 4 | 4 | 1 | 1 | 0 | 0 | 0 | 1 | – | – | – | – | – | – | – | – |
Cell Periphery | 9 | 5 | 1 | 3 | 2 | 1 | 2 | 2 | 5 | 5 | 0 | 0 | 2 | 1 | 2 | 3 | – | – | 0 | 0 | 0 | 2 | 2 | 9 |
Cytoplasm | 5 | 27 | 56 | 80 | 54 | 32 | 31 | 29 | 11 | 6 | 3 | 2 | 388 | 548 | 407 | 2 | – | – | 1 | 5 | 5 | 66 | 73 | 83 |
Endoplasmic Reticulum | 0 | 7 | 7 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 7 | 0 | – | – | 4 | 1 | 1 | 7 | 7 | 6 |
Endosome | 58 | 9 | 0 | 3 | 11 | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 14 | 68 | 71 | 5 | – | – | 33 | 35 | 14 | 38 | 50 | 64 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | – | – | 6 | 6 | 1 | 9 | 13 | 3 |
Mitochondria | 89 | 299 | 141 | 124 | 213 | 296 | 363 | 286 | 267 | 292 | 45 | 74 | 57 | 121 | 161 | 3 | – | – | 251 | 255 | 160 | 302 | 359 | 309 |
Nucleus | 5 | 1 | 0 | 0 | 3 | 5 | 7 | 4 | 3 | 7 | 0 | 1 | 0 | 1 | 2 | 1 | – | – | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 4 | 3 | 0 | 2 | 6 | 11 | 35 | 18 | 21 | 23 | 2 | 11 | 0 | 0 | 2 | 3 | – | – | 1 | 0 | 0 | 2 | 4 | 5 |
Nucleolus | 9 | 2 | 0 | 0 | 2 | 12 | 17 | 23 | 18 | 30 | 2 | 6 | 1 | 4 | 1 | 6 | – | – | 1 | 1 | 0 | 2 | 6 | 5 |
Peroxisomes | 41 | 5 | 4 | 13 | 11 | 5 | 3 | 8 | 3 | 6 | 1 | 0 | 5 | 43 | 49 | 16 | – | – | 13 | 6 | 13 | 19 | 15 | 13 |
SpindlePole | 14 | 5 | 4 | 17 | 25 | 10 | 19 | 17 | 4 | 10 | 3 | 3 | 14 | 35 | 20 | 8 | – | – | 2 | 3 | 5 | 44 | 45 | 44 |
Vac/Vac Membrane | 5 | 45 | 3 | 2 | 14 | 10 | 10 | 10 | 12 | 11 | 3 | 1 | 14 | 23 | 24 | 4 | – | – | 6 | 26 | 3 | 19 | 14 | 42 |
Unique Cell Count | 187 | 340 | 196 | 200 | 293 | 328 | 404 | 339 | 289 | 315 | 49 | 81 | 463 | 777 | 679 | 40 | 333 | 358 | 222 | 575 | 659 | 703 | ||
Labelled Cell Count | 242 | 426 | 232 | 261 | 364 | 402 | 523 | 435 | 384 | 426 | 62 | 102 | 507 | 865 | 761 | 55 | 333 | 358 | 222 | 575 | 659 | 703 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.3 | 3.5 | 3.5 | 4.0 | 3.8 | 3.9 | 5.3 | 6.6 | 7.3 | 8.9 | 8.1 | 4.0 | 4.0 | 4.2 | 9.9 | – | – | 4.1 | 4.2 | 4.7 |
Std Deviation (1e-4) | 1.2 | 0.8 | 0.8 | 2.0 | 1.5 | 1.5 | 1.9 | 3.4 | 5.3 | 5.8 | 7.2 | 5.4 | 1.0 | 1.0 | 1.2 | 3.6 | – | – | 1.5 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.2 | 0.11 | 0.14 | 0.6 | 0.92 | 1.06 | 1.34 | 1.21 | 0.19 | 0.19 | 0.27 | 1.5 | – | – | 0.21 | 0.26 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.3458 | 12.1385 | 12.0161 | 10.9213 | 14.0489 | 12.2138 | 7.6188 | 8.202 | 8.1393 | 9.2764 | 6.8666 | 6.2583 | 7.2306 | 10.9245 | 8.7479 | 8.8982 | 8.7716 | 9.165 |
Actin | 0.0235 | 0.0028 | 0.0175 | 0.0012 | 0.0017 | 0.0032 | 0.0294 | 0.0865 | 0.022 | 0.0002 | 0.0004 | 0.0325 | 0.0968 | 0.0036 | 0.0588 | 0.0002 | 0.0011 | 0.0102 |
Bud | 0.0004 | 0.0001 | 0.0007 | 0.0009 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0012 | 0 | 0.0001 | 0.001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0004 |
Bud Neck | 0.0045 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0003 |
Bud Periphery | 0.001 | 0.0002 | 0.0012 | 0.0051 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0016 | 0.0015 | 0.0002 | 0.0002 | 0.0022 | 0.0003 | 0.0014 | 0.0003 | 0.0024 | 0.0006 |
Bud Site | 0.0029 | 0.0002 | 0.0042 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.0007 | 0.0061 | 0.0011 | 0.0001 | 0.0002 | 0.0024 | 0.0006 | 0.002 | 0.0001 | 0.0001 | 0.0004 |
Cell Periphery | 0.0015 | 0.0001 | 0.0005 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0089 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0017 | 0.0001 |
Cytoplasm | 0.0117 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 |
Cytoplasmic Foci | 0.0106 | 0.0018 | 0.0037 | 0.0002 | 0.0005 | 0.0003 | 0.0167 | 0.0039 | 0.0012 | 0.0117 | 0.0037 | 0.0013 | 0.0286 | 0.0077 | 0.0009 | 0.0016 | 0.0005 | 0.0015 |
Eisosomes | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0018 | 0.0004 | 0.0041 | 0 | 0.0001 | 0.0003 | 0.0011 | 0.0002 | 0.0012 | 0 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.0016 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0009 | 0 | 0.0004 | 0.0002 | 0.0063 | 0 |
Endosome | 0.0165 | 0.0061 | 0.0064 | 0.0011 | 0.001 | 0.0026 | 0.0092 | 0.0086 | 0.0027 | 0.0431 | 0.0022 | 0.0021 | 0.0176 | 0.0035 | 0.0018 | 0.0748 | 0.0071 | 0.0025 |
Golgi | 0.0192 | 0.0232 | 0.0047 | 0.0009 | 0.0028 | 0.0269 | 0.0182 | 0.0144 | 0.0162 | 0.0213 | 0.0019 | 0.0152 | 0.0521 | 0.0041 | 0.0043 | 0.0248 | 0.0045 | 0.0052 |
Lipid Particles | 0.0244 | 0.0028 | 0.0053 | 0.0023 | 0.0297 | 0.0071 | 0.0435 | 0.0017 | 0.0041 | 0.0038 | 0.0595 | 0.0045 | 0.1945 | 0.0081 | 0.0016 | 0.0014 | 0.0009 | 0.0142 |
Mitochondria | 0.8023 | 0.9421 | 0.8924 | 0.9785 | 0.948 | 0.9524 | 0.7534 | 0.8474 | 0.9189 | 0.8961 | 0.9162 | 0.9173 | 0.4771 | 0.9122 | 0.9133 | 0.8799 | 0.9585 | 0.9348 |
None | 0.0117 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0015 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Nuclear Periphery | 0.0063 | 0 | 0.0034 | 0.0001 | 0.0007 | 0.0002 | 0.034 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0034 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0001 |
Nucleolus | 0.0209 | 0.0001 | 0.0005 | 0 | 0.0008 | 0 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0004 | 0 | 0.0003 | 0.0077 | 0.0001 | 0.0001 | 0 | 0.0003 |
Nucleus | 0.0027 | 0 | 0.0011 | 0.0001 | 0.0005 | 0 | 0.0009 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Peroxisomes | 0.0273 | 0.0121 | 0.0236 | 0.0043 | 0.006 | 0.0034 | 0.0817 | 0.0349 | 0.0034 | 0.0035 | 0.0129 | 0.0248 | 0.1146 | 0.0478 | 0.003 | 0.0003 | 0.0064 | 0.0221 |
Punctate Nuclear | 0.0018 | 0 | 0.0047 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0025 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0032 | 0.0009 | 0.0106 | 0.0017 | 0.0011 | 0.0005 | 0.0008 | 0.0003 | 0.0015 | 0.0075 | 0.0009 | 0.0002 | 0.0006 | 0.0014 | 0.004 | 0.0088 | 0.0041 | 0.0018 |
Vacuole Periphery | 0.0058 | 0.0073 | 0.0182 | 0.0026 | 0.0063 | 0.0026 | 0.0073 | 0.0004 | 0.0081 | 0.0069 | 0.0011 | 0.001 | 0.0016 | 0.0021 | 0.0046 | 0.0066 | 0.0039 | 0.0051 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.0312 | 19.3407 | 12.4998 | 14.1078 | 18.55 | 25.111 | 14.0357 | 11.9213 | 17.4461 | 15.6211 |
Translational Efficiency | 1.0504 | 0.7351 | 1.0023 | 1.241 | 1.1854 | 1.2992 | 0.9941 | 1.5887 | 1.3232 | 1.3012 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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