Standard name
Human Ortholog
Description Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.07 0.08 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.23 0.21 0.18 0.23 0.25 0.26 0.27 0.29 0.31 0.27 0.22 0.27 0.4 0.44 0.47 0.31 0.3 0.42 0.06 0.07 0.21 0.21 0.16 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0.07 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Mitochondria 0 0 0 0 0 0.07 0.27 0.09 0.26 0.19 0.44 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.2 0.23 0.29 0.5 0.23 0.28 0.26 0.3 0.29 0.32 0.31 0.34 0.16 0.2 0.18 0.11 0.09 0.08 0.66 0.68 0.47 0.42 0.41 0.35
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.75 0.6 0.38 0.58 0.48 0.31 0.34 0.27 0.25 0.19 0.27 0.54 0.55 0.5 0.7 0.71 0.58 0.11 0.12 0.14 0.18 0.24 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 2 0 0 0
Bud 0 0 0 0 0 3 6 4 4 11 3 2 0 0 0 1 0 0 10 19 9 7 15 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 1
Bud Site 0 0 0 0 0 0 3 0 3 0 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 1 2 2 0 0 0 0 0 0
Cytoplasm 26 47 36 38 65 75 72 87 102 88 40 54 40 69 67 92 72 98 8 18 50 23 30 19
Endoplasmic Reticulum 0 0 0 0 1 1 0 0 1 1 0 0 1 0 0 11 5 8 1 0 3 1 3 3
Endosome 0 1 2 0 13 21 6 16 4 15 0 0 1 2 1 10 8 2 1 3 7 1 3 5
Golgi 0 4 0 0 1 0 0 0 0 0 0 0 3 3 1 5 2 4 0 0 1 0 3 9
Mitochondria 4 1 1 1 1 20 73 27 85 64 79 49 1 0 0 3 4 2 1 0 7 1 0 0
Nucleus 23 50 59 84 59 81 69 91 97 106 56 67 16 31 26 34 23 18 93 179 114 45 78 66
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0 0 1
Nucleolus 0 1 0 1 1 0 5 1 2 4 0 1 1 0 0 1 1 2 2 2 3 2 2 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 1 1 0 1 3 4 2 2 5
Vac/Vac Membrane 76 165 121 64 152 138 84 103 88 83 35 53 55 86 71 209 173 136 15 32 34 19 44 55
Unique Cell Count 113 220 201 168 261 285 269 299 329 330 180 198 101 156 143 297 244 234 143 264 245 110 189 189
Labelled Cell Count 129 269 219 189 294 340 319 329 386 375 214 229 118 191 166 370 292 272 143 264 245 110 189 189


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.1 16.6 10.3 10.5 9.4 7.7 6.5 7.4 6.1 6.5 5.6 6.1 13.6 14.0 13.0 11.6 11.5 10.8 22.5 30.4 40.0
Std Deviation (1e-4) 3.0 3.8 2.0 1.9 1.6 1.9 1.5 1.6 1.4 1.5 1.3 1.2 3.2 3.0 2.6 2.1 2.2 2.0 8.1 10.8 13.9
Intensity Change (Log2) – – – 0.03 -0.13 -0.41 -0.66 -0.47 -0.75 -0.67 -0.88 -0.76 0.41 0.44 0.34 0.18 0.16 0.08 1.13 1.56 1.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.8 2.2 2.3 2.8 3.3 2.3 1.1 2.2 4.1 5.4 5.8 3.3 2.8 5.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.4 3.3 0 2.6 0 2.3 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.5 7.8 4.1 7.7 6.4 10.4 7.3 0 0 0 0 0 0
Nucleus 4.1 -1.6 -0.2 -0.9 0.3 0 0.7 0.4 1.0 -2.6 -2.0 -2.4 -5.0 -5.4 -5.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.2 -0.4 -2.6 -6.3 -5.7 -7.6 -8.1 -8.1 -6.7 -1.0 -1.0 -1.9 2.4 2.4 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7127 10.1855 8.24 8.5165 7.4308 8.5179 7.4669 9.154 8.1443 7.7605 6.7706 7.6637 5.3523 7.1821 6.934 4.9172 4.0845 6.6238
Actin 0.0095 0.0011 0.0022 0.0028 0.0044 0.0016 0.0025 0.0001 0.0003 0 0.0042 0 0.0686 0.0019 0.034 0.0018 0.0172 0.0005
Bud 0.0011 0.0001 0.0012 0.0003 0.0009 0.0002 0.0003 0.0001 0.0001 0 0.0009 0 0.0027 0.0004 0.0003 0.003 0.0009 0.0002
Bud Neck 0.0047 0.0003 0.0005 0.0015 0.0186 0.0029 0.0011 0.0003 0.0003 0.0002 0.0017 0.0016 0.0027 0.0003 0.0004 0.0072 0.0011 0.0017
Bud Periphery 0.0023 0 0.0005 0.0004 0.0031 0.0002 0.0006 0.0001 0.0002 0 0.0005 0 0.0027 0.0004 0.0002 0.0041 0.0014 0.0001
Bud Site 0.0022 0.0014 0.001 0.0006 0.0016 0.0003 0.0007 0.0003 0.0003 0 0.0011 0 0.0228 0.0008 0.0006 0.0009 0.0026 0.0002
Cell Periphery 0.0006 0 0.0001 0.0001 0.0007 0.0001 0.0001 0 0 0 0.0001 0 0.0007 0.0001 0.0001 0.0002 0.0003 0
Cytoplasm 0.041 0.0263 0.0297 0.0058 0.0355 0.0226 0.0261 0.0284 0.0106 0.0032 0.0119 0.0095 0.0405 0.035 0.0387 0.0423 0.0124 0.0359
Cytoplasmic Foci 0.0178 0.0033 0.0076 0.0042 0.0291 0.0075 0.0053 0.0002 0.0009 0 0.0007 0.0001 0.0528 0.0036 0.0092 0.004 0.0242 0.0213
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0.0001 0 0.0004 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0127 0.0013 0.0019 0.0003 0.0095 0.0018 0.0015 0.0005 0.0005 0 0.0024 0.0002 0.0096 0.0019 0.0034 0.0049 0.0037 0.0009
Endosome 0.031 0.0042 0.0108 0.0167 0.0332 0.0092 0.004 0.0002 0.0022 0 0.0157 0.0003 0.0489 0.0086 0.0201 0.0813 0.0842 0.0019
Golgi 0.0122 0.0005 0.0051 0.0168 0.0029 0.0065 0.0007 0 0.0007 0 0.0017 0 0.0149 0.0075 0.0156 0.0102 0.0362 0.0006
Lipid Particles 0.0077 0.0016 0.0024 0.0014 0.0096 0.0053 0.0017 0 0.0004 0 0.0019 0 0.0303 0.0005 0.0023 0.002 0.0356 0.0026
Mitochondria 0.0165 0.0003 0.0073 0.0157 0.0028 0.0027 0.0042 0.0001 0.0024 0 0.0014 0.0001 0.0184 0.0029 0.0391 0.0129 0.1599 0.0007
None 0.0111 0.0027 0.0121 0.0001 0.0039 0.0004 0.0007 0.0001 0.0005 0 0.0011 0.0001 0.0401 0.033 0.0003 0.001 0.0007 0.0023
Nuclear Periphery 0.0404 0.0145 0.0099 0.0041 0.0191 0.0081 0.0108 0.0077 0.0046 0.0021 0.0319 0.0065 0.0339 0.0131 0.0086 0.0107 0.0119 0.0118
Nucleolus 0.0028 0.0015 0.0017 0.0009 0.0037 0.0023 0.002 0.0011 0.0012 0.0006 0.002 0.0016 0.0081 0.0011 0.0005 0.0014 0.0012 0.0016
Nucleus 0.7524 0.9343 0.897 0.9215 0.7943 0.9172 0.9253 0.9506 0.9639 0.993 0.9128 0.972 0.5595 0.8844 0.8224 0.7923 0.586 0.9102
Peroxisomes 0.0072 0.0006 0.0017 0.0008 0.0039 0.0043 0.0067 0 0.0011 0 0.0003 0 0.0129 0.0003 0.002 0.0005 0.0063 0.0029
Punctate Nuclear 0.0161 0.0045 0.0048 0.0032 0.0159 0.0027 0.0029 0.0091 0.0088 0.0008 0.0053 0.0075 0.008 0.0022 0.0011 0.001 0.0084 0.0034
Vacuole 0.0086 0.0013 0.002 0.0023 0.0063 0.0035 0.0018 0.0009 0.0009 0 0.001 0.0004 0.0144 0.0015 0.0005 0.0106 0.0037 0.0009
Vacuole Periphery 0.0024 0.0002 0.0006 0.0005 0.0008 0.0007 0.0009 0.0001 0.0002 0 0.0012 0.0001 0.0069 0.0005 0.0004 0.0078 0.002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5848 8.3591 14.4733 14.0482 21.5535 8.3073 14.7908 15.2119 11.9107 16.4056
Translational Efficiency 0.943 1.4279 0.8366 0.8677 0.9195 1.3604 0.6301 0.762 0.9311 1.0398

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis
Localization
Cell Percentages nucleus (24%), cytoplasm (9%), mixed (66%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fus3

Fus3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fus3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available