Standard name
Human Ortholog
Description Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.79 0.77 0.73 0.77 0.49 0.43 0.42 0.38 0.3 0.28 0.25 0.78 0.83 0.81 0.73 0.65 0.7 0.61 0.55 0.45 0.71 0.75 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.32 0.05 0.07 0.3 0.16 0.51 0.52 0.62 0.7 0.73 0.73 0.78 0 0 0 0 0 0 0.05 0.05 0.17 0.06 0 0
Nucleus 0 0 0 0 0 0.08 0.1 0.06 0.11 0.15 0.14 0.14 0.07 0.06 0 0.05 0.08 0.08 0.05 0.11 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.25 0.23 0.11 0.16 0.12 0.16 0.09 0.08 0.08 0.11 0.14 0.21 0.15 0.2 0.31 0.38 0.29 0.19 0.2 0.18 0.12 0.07 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 0 0 0
Bud 1 0 0 0 1 1 9 3 4 8 2 6 0 1 2 0 0 0 2 5 5 0 1 3
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 3 4 2 0 2 1
Bud Site 0 0 0 1 0 1 1 0 3 2 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 2 0 0 1 0 0 0 0 0 1 2 2 0 1 0 0 0 0
Cytoplasm 276 312 188 116 138 173 184 144 110 114 72 70 110 167 220 406 203 174 244 255 120 67 85 79
Endoplasmic Reticulum 0 1 2 1 2 0 0 0 1 0 1 1 0 1 0 43 24 15 1 0 5 2 0 0
Endosome 0 0 8 1 4 3 3 5 0 0 0 0 2 1 1 16 3 2 3 3 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2 1 3 2 1 2 0 0 1
Mitochondria 116 21 17 47 29 180 222 210 206 281 189 214 1 2 7 23 7 6 18 21 46 5 4 2
Nucleus 3 7 2 4 4 30 43 21 32 59 37 39 10 13 10 27 25 19 19 48 13 3 3 0
Nuclear Periphery 0 0 1 1 0 2 3 1 0 8 5 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 5 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
SpindlePole 0 0 1 2 0 1 1 8 0 1 5 2 0 0 0 2 2 0 8 13 3 2 2 1
Vac/Vac Membrane 41 99 56 17 28 43 67 29 23 31 28 39 30 30 53 169 120 72 77 90 49 11 7 17
Unique Cell Count 362 396 244 158 179 354 424 339 293 384 258 275 141 202 271 554 314 248 402 466 269 94 114 114
Labelled Cell Count 437 440 275 191 207 434 540 423 379 505 342 372 156 215 294 689 387 294 402 466 269 94 114 114


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.2 4.2 3.6 4.2 3.4 3.3 3.3 3.2 2.9 3.1 2.9 4.6 4.5 4.4 5.5 6.1 5.6 4.3 4.3 5.6
Std Deviation (1e-4) 0.7 0.8 1.2 1.3 1.9 1.3 1.7 1.1 1.3 0.8 1.0 1.1 1.3 1.6 1.4 1.3 1.9 1.4 1.5 1.7 2.3
Intensity Change (Log2) – – – -0.23 -0.02 -0.33 -0.36 -0.35 -0.41 -0.57 -0.47 -0.54 0.13 0.07 0.06 0.36 0.53 0.4 0.01 0.01 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.2 3.8 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.287 -0.2139 -0.0566 -0.2207 0.1189 0.0649 2.4349 2.8634 2.7501 2.3957 2.8884 2.9922 3.3628 3.3052 3.1097 3.2251 4.6332 3.2762
Actin 0.0534 0.0001 0.0338 0.0008 0.0038 0.0004 0.0026 0.0003 0.0036 0.0366 0.0049 0.0012 0.0236 0.0002 0.001 0.0001 0.0003 0.0055
Bud 0.0003 0.0004 0.0003 0.0001 0.0007 0.0001 0.0005 0.0006 0.0009 0.0002 0.0007 0.0006 0.0013 0.0011 0.003 0 0.0001 0.0001
Bud Neck 0.0028 0.0005 0.001 0.0009 0.0151 0.0024 0.0016 0.0006 0.0011 0.0006 0.0044 0.0049 0.0123 0.0002 0.0004 0.0005 0.0021 0.0098
Bud Periphery 0.0008 0.0017 0.0003 0 0.0012 0.0001 0.0011 0.0006 0.0017 0.0004 0.0024 0.0021 0.0017 0.0004 0.0011 0 0.0001 0.0001
Bud Site 0.0024 0.0017 0.0037 0.0002 0.0277 0.0003 0.0023 0.0106 0.002 0.0006 0.0153 0.0003 0.0038 0.0007 0.0118 0.0001 0.0002 0.0001
Cell Periphery 0.0008 0.0008 0.0002 0.0001 0.0006 0.0001 0.0001 0.0002 0.0002 0.0001 0.0011 0.0001 0.0004 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.1151 0.115 0.118 0.248 0.034 0.1429 0.1276 0.1683 0.1091 0.1107 0.1812 0.1477 0.1142 0.2008 0.1273 0.1604 0.1411 0.2143
Cytoplasmic Foci 0.0174 0.0067 0.011 0.0048 0.0054 0.0164 0.0142 0.0053 0.0046 0.0027 0.0104 0.007 0.0094 0.0045 0.0108 0.0044 0.0044 0.0113
Eisosomes 0.0014 0.0002 0.0003 0.0002 0.0005 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0038 0.0028 0.0072 0.0134 0.0009 0.0041 0.0048 0.0022 0.0023 0.0019 0.0028 0.0014 0.0062 0.0034 0.0034 0.0018 0.0026 0.0029
Endosome 0.0107 0.0016 0.0103 0.0047 0.002 0.0034 0.0125 0.0029 0.005 0.0025 0.0096 0.0022 0.0124 0.0029 0.0024 0.0026 0.002 0.0042
Golgi 0.0071 0.0001 0.0031 0.0002 0.0013 0.0001 0.0014 0.0002 0.0008 0.0012 0.0018 0.0002 0.0018 0.0001 0.0003 0.0001 0.0001 0.0004
Lipid Particles 0.0121 0.0004 0.0054 0.0006 0.0016 0.0008 0.0039 0.0004 0.0014 0.001 0.0046 0.0005 0.0032 0.0004 0.001 0.0002 0.0006 0.0006
Mitochondria 0.003 0.0004 0.016 0.0011 0.0043 0.0008 0.0026 0.001 0.0064 0.0012 0.0022 0.0008 0.0025 0.0004 0.0013 0.0006 0.0004 0.0006
None 0.5653 0.6267 0.5249 0.4685 0.857 0.5073 0.5584 0.5511 0.552 0.5364 0.4076 0.4841 0.5958 0.6979 0.6458 0.6921 0.7235 0.4436
Nuclear Periphery 0.0098 0.0028 0.0219 0.0131 0.0023 0.0076 0.0116 0.0073 0.0139 0.0107 0.0124 0.0068 0.0221 0.0059 0.0092 0.0054 0.0088 0.0087
Nucleolus 0.0043 0.007 0.0033 0.006 0.0045 0.0049 0.0036 0.0037 0.0053 0.0023 0.01 0.0052 0.0101 0.0017 0.002 0.0035 0.0018 0.0026
Nucleus 0.1175 0.2031 0.193 0.2152 0.0093 0.2851 0.2143 0.2069 0.2441 0.261 0.2355 0.2689 0.1264 0.0597 0.1586 0.0978 0.0878 0.2293
Peroxisomes 0.0324 0.0002 0.0303 0.0002 0.0046 0.0007 0.0081 0.002 0.0014 0.001 0.0214 0.0009 0.0015 0.0001 0.0009 0.0001 0.0001 0.0002
Punctate Nuclear 0.0103 0.0208 0.0118 0.0197 0.0217 0.0193 0.024 0.0329 0.0409 0.0279 0.0534 0.0639 0.0438 0.0159 0.018 0.0291 0.0228 0.0642
Vacuole 0.0281 0.0067 0.0035 0.0018 0.0012 0.0028 0.0036 0.0026 0.0021 0.0007 0.0175 0.0011 0.0053 0.0035 0.0011 0.0009 0.001 0.0012
Vacuole Periphery 0.0014 0.0004 0.0008 0.0004 0.0002 0.0003 0.0009 0.0003 0.0007 0.0003 0.0006 0.0002 0.0018 0.0002 0.0003 0.0002 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.9417 5.0486 2.3414 5.8033 5.0341 2.8222 4.3071 3.6606 5.2829 6.3054
Translational Efficiency 0.7118 0.5899 1.2074 0.6926 0.7062 1.6739 0.6828 1.1843 0.6121 0.5594

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
217 1516 470 1987 1662 1421 1420 51 1879 2937 1890 2038

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 639.78 685.57 769.51 691.91 644.19 664.27 865.70 771.08 643.68 675.26 841.78 693.89
Standard Deviation 87.21 97.92 85.04 80.05 75.41 102.33 109.94 108.19 76.87 100.64 112.29 81.81
Intensity Change Log 2 — 0.099728 0.266364 0.113008 — 0.044283 0.426381 0.259394 — 0.072177 0.348864 0.188308

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000542 0.000133 0.000231 0.000096 0.000041 0.000193 0.000245 0.001285 0.000099 0.000162 0.000242 0.000126
Bud Neck 0.001793 0.013292 0.003456 0.005329 0.005170 0.017545 0.004496 0.025743 0.004780 0.015350 0.004237 0.005840
Bud Site 0.001561 0.006075 0.002460 0.005103 0.001500 0.007157 0.007505 0.102577* 0.001507 0.006598 0.006251 0.007543
Cell Periphery 0.000143 0.000103 0.000087 0.000151 0.000104 0.000276 0.000151 0.003158 0.000109 0.000187 0.000135 0.000226
Cytoplasm 0.328555* 0.281269* 0.048635 0.275418* 0.356370* 0.326759* 0.144809* 0.401850* 0.353158* 0.303278* 0.120893* 0.278582*
Cytoplasmic Foci 0.128476 0.063556 0.003289 0.005262 0.080103 0.143336 0.003077 0.035950 0.085690 0.102155 0.003130 0.006030
Eisosomes 0.000113 0.000041 0.000032 0.000027 0.000058 0.000120 0.000032 0.000099 0.000064 0.000079 0.000032 0.000028
Endoplasmic Reticulum 0.001274 0.001667 0.003844 0.000787 0.000519 0.001195 0.004001 0.002229 0.000606 0.001438 0.003962 0.000823
Endosome 0.003552 0.004102 0.001017 0.000791 0.001747 0.008951 0.001046 0.013731 0.001956 0.006448 0.001038 0.001115
Golgi 0.001541 0.000974 0.000051 0.000166 0.000347 0.001927 0.000049 0.001855 0.000485 0.001435 0.000049 0.000208
Lipid Particles 0.005351 0.004158 0.000889 0.000418 0.005311 0.011435 0.000765 0.004282 0.005316 0.007679 0.000796 0.000514
Mitochondria 0.005743 0.011341 0.000719 0.000626 0.001626 0.013548 0.000727 0.001648 0.002101 0.012409 0.000725 0.000651
Mitotic Spindle 0.003731 0.011572 0.001729 0.005261 0.001183 0.003138 0.003046 0.006180 0.001477 0.007492 0.002719 0.005284
None 0.005030 0.004145 0.005200 0.005799 0.005141 0.008229 0.006093 0.009878 0.005128 0.006121 0.005871 0.005901
Nuclear Periphery 0.000547 0.001719 0.001391 0.001506 0.000912 0.001312 0.001866 0.000821 0.000870 0.001522 0.001748 0.001489
Nuclear Periphery Foci 0.000337 0.000492 0.000321 0.000103 0.000180 0.000423 0.000515 0.002213 0.000198 0.000459 0.000467 0.000156
Nucleolus 0.000925 0.002751 0.001802 0.001039 0.001290 0.004403 0.000642 0.000949 0.001248 0.003550 0.000931 0.001037
Nucleus 0.489339* 0.562202* 0.913123* 0.682805* 0.524530* 0.404966* 0.793896* 0.287547* 0.520466* 0.486127* 0.823545* 0.672914*
Peroxisomes 0.006039 0.004049 0.000282 0.000112 0.002709 0.010252 0.000117 0.001965 0.003094 0.007050 0.000158 0.000158
Vacuole 0.014878 0.024887 0.011200 0.008998 0.010899 0.033233 0.026803 0.091600 0.011358 0.028925 0.022923 0.011065
Vacuole Periphery 0.000529 0.001472 0.000240 0.000202 0.000258 0.001602 0.000119 0.004440 0.000289 0.001535 0.000149 0.000308

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.03 0.44 1.14 1.22 3.72 -6.88 -14.70 -4.27 -3.78 -3.20 -1.46 -5.57 -0.50 1.11 6.18
Bud Neck -10.24 -2.73 -5.67 6.18 -0.00 -7.65 -0.04 -1.40 -0.22 -1.40 -10.17 -0.82 -1.61 8.23 -0.96
Bud Site -3.12 -1.47 -2.59 0.59 -1.25 -4.32 -3.17 -3.40 -3.14 -3.18 -5.71 -3.48 -4.57 -0.32 -0.93
Cell Periphery 2.61 2.80 0.01 -2.33 -2.52 -3.93 -2.92 -2.41 -2.24 -2.37 -3.52 -1.96 -3.10 -0.55 -2.48
Cytoplasm 2.51 15.13 3.05 1.08 -28.18 3.40 28.85 -0.30 -0.99 -5.29 6.78 34.54 9.60 3.79 -23.14
Cytoplasmic Foci 5.67 11.48 11.33 18.80 -2.10 -10.83 24.13 5.45 11.17 -2.74 -3.80 26.52 25.41 32.68 -4.90
Eisosomes 7.28 7.09 8.26 3.29 2.76 -7.77 4.70 -2.72 0.79 -3.54 -3.33 7.35 7.30 8.76 3.28
Endoplasmic Reticulum -0.53 -4.12 0.39 5.19 9.30 -6.79 -21.65 -3.20 -2.53 2.24 -6.08 -21.11 -2.45 4.40 20.78
Endosome -0.63 2.18 2.91 8.39 2.84 -9.10 2.77 -2.01 -0.26 -2.13 -9.69 3.38 4.26 12.19 1.98
Golgi 0.68 1.81 1.69 3.58 -0.77 -5.81 3.35 -1.76 0.91 -2.30 -4.66 3.54 1.92 6.46 -1.10
Lipid Particles 0.75 2.95 3.46 6.78 5.35 -6.36 10.62 1.25 4.74 -1.79 -3.58 10.85 12.11 14.10 6.98
Mitochondria -1.33 1.23 1.29 7.06 0.79 -7.03 3.78 -0.01 6.92 -2.28 -8.36 2.61 2.92 10.41 1.46
Mitotic Spindle -2.57 0.44 -0.62 2.65 -1.68 -1.82 -3.30 -2.39 -1.63 -0.89 -4.80 -2.98 -2.85 1.29 -0.58
None 0.46 0.03 -0.34 -1.94 -0.63 -2.46 -0.35 -0.86 0.11 -0.79 -1.21 -0.20 -0.59 0.40 -0.47
Nuclear Periphery -9.99 -9.41 -10.93 0.72 0.52 -1.93 -16.31 -2.02 -0.80 2.99 -5.61 -17.35 -9.38 -1.10 8.04
Nuclear Periphery Foci -0.82 -1.52 1.18 2.48 2.20 -3.98 -9.07 -2.57 -2.22 -1.47 -2.90 -6.36 1.47 3.49 6.84
Nucleolus -4.11 -3.38 -0.38 3.89 2.95 -5.03 4.87 0.03 3.95 -1.42 -5.93 2.47 1.46 6.42 -0.26
Nucleus -3.04 -17.71 -8.77 -12.25 20.10 11.16 -26.02 3.84 1.24 9.36 3.90 -32.96 -17.11 -22.12 14.92
Peroxisomes 0.65 2.00 2.10 6.41 2.05 -6.29 5.92 2.04 7.18 -1.71 -4.64 5.87 5.95 11.50 0.99
Vacuole -4.14 -1.25 1.95 8.11 3.06 -11.39 -13.00 -4.36 -3.35 -2.27 -12.87 -12.94 -1.51 9.97 11.76
Vacuole Periphery -2.91 1.18 1.38 5.83 0.55 -8.56 3.10 -1.75 -0.96 -1.83 -8.86 2.76 0.17 8.59 -1.93
Standard name
Human Ortholog
Description Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
Localization
Cell Percentages nucleus (19%), cytoplasm (8%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Apn2

Apn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available