Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 3 | 1 | 1 | 1 | 8 | 11 | 15 | 8 | 9 | 7 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 39 | 11 | 9 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 6 | 4 | 7 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 6 | 7 | 12 | 11 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 6 | 3 | 1 | 3 | 1 | 4 | 1 | 3 | 1 | 0 | 1 | 7 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 21 | 101 | 47 | 55 | 130 | 77 | 56 | 64 | 52 | 55 | 44 | 40 | 30 | 93 | 98 | 73 | 64 | 60 | 15 | 56 | 23 | 12 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 2 | 0 | 4 | 4 | 2 | 0 | 0 | 0 | 1 | 4 | 3 | 0 | 1 | 2 | 3 | 0 | 0 |
Endosome | 4 | 9 | 9 | 7 | 14 | 8 | 8 | 7 | 0 | 8 | 2 | 3 | 3 | 22 | 18 | 5 | 5 | 4 | 1 | 4 | 3 | 8 | 23 | 38 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 8 |
Mitochondria | 2 | 3 | 4 | 4 | 17 | 66 | 60 | 109 | 95 | 92 | 108 | 73 | 0 | 2 | 0 | 4 | 3 | 5 | 0 | 3 | 1 | 5 | 11 | 22 |
Nucleus | 74 | 192 | 101 | 114 | 75 | 178 | 214 | 222 | 174 | 183 | 187 | 159 | 117 | 225 | 152 | 90 | 86 | 100 | 74 | 195 | 82 | 188 | 175 | 190 |
Nuclear Periphery | 0 | 1 | 4 | 7 | 2 | 16 | 19 | 14 | 12 | 24 | 10 | 7 | 1 | 8 | 8 | 3 | 1 | 4 | 1 | 0 | 3 | 3 | 14 | 15 |
Nucleolus | 10 | 12 | 27 | 39 | 51 | 96 | 101 | 153 | 139 | 139 | 139 | 128 | 10 | 36 | 38 | 15 | 16 | 17 | 8 | 21 | 55 | 64 | 128 | 118 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 2 | 1 | 3 | 0 | 3 | 0 | 0 | 0 | 1 | 2 | 13 | 12 | 8 | 10 | 18 |
Vac/Vac Membrane | 4 | 22 | 8 | 13 | 19 | 18 | 15 | 25 | 12 | 20 | 9 | 21 | 7 | 36 | 28 | 27 | 28 | 19 | 1 | 11 | 3 | 28 | 74 | 89 |
Unique Cell Count | 109 | 330 | 189 | 210 | 282 | 389 | 403 | 505 | 391 | 429 | 415 | 366 | 161 | 405 | 322 | 209 | 194 | 200 | 115 | 356 | 204 | 338 | 450 | 529 |
Labelled Cell Count | 115 | 343 | 204 | 242 | 318 | 481 | 496 | 629 | 505 | 560 | 523 | 446 | 169 | 426 | 343 | 228 | 215 | 220 | 115 | 356 | 204 | 338 | 450 | 529 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 9.2 | 8.9 | 8.9 | 8.5 | 7.3 | 7.9 | 7.7 | 7.4 | 7.6 | 7.5 | 7.6 | 9.9 | 10.1 | 10.5 | 13.0 | 13.0 | 12.9 | 9.1 | 9.8 | 10.0 |
Std Deviation (1e-4) | 2.3 | 1.8 | 1.9 | 2.5 | 2.4 | 1.9 | 2.3 | 2.0 | 2.0 | 2.2 | 1.9 | 2.1 | 2.0 | 2.3 | 2.5 | 3.3 | 3.1 | 3.3 | 1.7 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.05 | -0.28 | -0.17 | -0.2 | -0.26 | -0.22 | -0.24 | -0.21 | 0.17 | 0.19 | 0.24 | 0.56 | 0.55 | 0.55 | 0.04 | 0.14 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 4.7 | -1.4 | -3.3 | -3.9 | -3.5 | -3.7 | -4.5 | -4.3 | -1.4 | -0.5 | 1.3 | 2.2 | 1.8 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.7 | 0.1 | -1.8 | -1.9 | 0 | 0 | -2.0 | 0 | 0 | -1.5 | 0.3 | 0.4 | -1.3 | -1.1 | -1.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.0 | 5.1 | 4.7 | 6.2 | 6.7 | 6.1 | 7.0 | 5.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.2 | -5.9 | -1.7 | -0.1 | -2.2 | -2.0 | -2.5 | -1.9 | -2.2 | 3.7 | 0.5 | -1.4 | -2.1 | -1.8 | -0.7 |
Nuclear Periphery | 0.7 | 0 | 1.2 | 1.5 | 0 | 0.7 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.2 | 1.1 | 2.9 | 3.0 | 4.3 | 5.3 | 4.7 | 4.9 | 5.1 | -2.4 | -2.0 | -0.8 | -2.3 | -1.9 | -1.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.9 | 1.1 | 0.2 | -0.3 | 0.4 | -0.7 | 0.2 | -1.4 | 0.8 | 0.1 | 2.0 | 1.9 | 3.1 | 3.4 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4955 | 3.1073 | 3.2297 | 2.8103 | 2.1096 | 3.473 | 5.1991 | 5.4903 | 5.204 | 4.0687 | 3.75 | 4.9087 | 3.0169 | 4.6416 | 3.1649 | 2.9764 | 3.09 | 3.915 |
Actin | 0.0243 | 0.0022 | 0.0025 | 0.0029 | 0.0023 | 0.0005 | 0.0164 | 0.0036 | 0.01 | 0.0193 | 0.047 | 0.0021 | 0.0012 | 0.0008 | 0.0479 | 0.0001 | 0.0006 | 0.0123 |
Bud | 0.0001 | 0.0192 | 0.0362 | 0.067 | 0.0375 | 0.0023 | 0.0015 | 0.0215 | 0.0455 | 0.0774 | 0.0576 | 0.0083 | 0.0001 | 0.0035 | 0.0228 | 0.0003 | 0.01 | 0.0011 |
Bud Neck | 0.0003 | 0.0019 | 0.0041 | 0.0192 | 0.0046 | 0.0067 | 0.0025 | 0.0027 | 0.0058 | 0.0051 | 0.0034 | 0.0081 | 0.0003 | 0.0008 | 0.002 | 0.0002 | 0.0021 | 0.002 |
Bud Periphery | 0.0002 | 0.0091 | 0.0251 | 0.0549 | 0.0522 | 0.0027 | 0.0021 | 0.0127 | 0.0384 | 0.0553 | 0.0703 | 0.0096 | 0.0001 | 0.0016 | 0.0146 | 0.0001 | 0.0089 | 0.0005 |
Bud Site | 0.0003 | 0.0339 | 0.0254 | 0.0315 | 0.0059 | 0.0024 | 0.0062 | 0.0563 | 0.0363 | 0.0207 | 0.0095 | 0.0013 | 0.0007 | 0.0037 | 0.0118 | 0.0002 | 0.0021 | 0.0004 |
Cell Periphery | 0 | 0.0008 | 0.0013 | 0.0033 | 0.0028 | 0.0004 | 0.0005 | 0.0014 | 0.0018 | 0.0013 | 0.0021 | 0.0004 | 0 | 0.0001 | 0.0005 | 0 | 0.0009 | 0.0001 |
Cytoplasm | 0.0276 | 0.2454 | 0.4469 | 0.4951 | 0.7209 | 0.127 | 0.0144 | 0.1572 | 0.2807 | 0.5977 | 0.5157 | 0.0821 | 0.0064 | 0.2326 | 0.5326 | 0.9597 | 0.8993 | 0.1264 |
Cytoplasmic Foci | 0.0035 | 0.0082 | 0.0201 | 0.0097 | 0.0206 | 0.0049 | 0.0134 | 0.012 | 0.0222 | 0.0226 | 0.0421 | 0.0036 | 0.0518 | 0.001 | 0.0284 | 0.0114 | 0.0057 | 0.0082 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.002 | 0.0078 | 0.0064 | 0.0065 | 0.0036 | 0.0016 | 0.0068 | 0.0035 | 0.0086 | 0.0058 | 0.0072 | 0.002 | 0.0002 | 0.0011 | 0.0119 | 0.0012 | 0.0135 | 0.0007 |
Endosome | 0.0028 | 0.0032 | 0.0278 | 0.0241 | 0.1043 | 0.0073 | 0.0125 | 0.0126 | 0.0299 | 0.0327 | 0.0486 | 0.0085 | 0.0033 | 0.0004 | 0.0364 | 0.0179 | 0.0078 | 0.0064 |
Golgi | 0.0013 | 0.0006 | 0.001 | 0.0007 | 0.0037 | 0.0002 | 0.0057 | 0.0013 | 0.0029 | 0.0144 | 0.0245 | 0.0012 | 0.0032 | 0.0001 | 0.0025 | 0.0006 | 0.0003 | 0.0031 |
Lipid Particles | 0.0036 | 0.0018 | 0.0025 | 0.0002 | 0.0002 | 0.0003 | 0.0151 | 0.0009 | 0.013 | 0.0156 | 0.062 | 0.001 | 0.009 | 0.0001 | 0.0105 | 0 | 0.0002 | 0.0052 |
Mitochondria | 0.0008 | 0.001 | 0.0017 | 0.0018 | 0.0007 | 0.0004 | 0.0071 | 0.0096 | 0.0094 | 0.0076 | 0.0067 | 0.0009 | 0.0013 | 0.001 | 0.0043 | 0.0001 | 0.0003 | 0.0008 |
None | 0.0071 | 0.0103 | 0.0087 | 0.0358 | 0.003 | 0.0005 | 0.0033 | 0.0129 | 0.0067 | 0.0087 | 0.014 | 0.001 | 0.0006 | 0.0009 | 0.091 | 0.0024 | 0.004 | 0.001 |
Nuclear Periphery | 0.0094 | 0.0183 | 0.0148 | 0.0099 | 0.0019 | 0.0046 | 0.0125 | 0.0169 | 0.0351 | 0.0084 | 0.0123 | 0.0133 | 0.0022 | 0.0165 | 0.035 | 0.0004 | 0.0082 | 0.0062 |
Nucleolus | 0.0194 | 0.0391 | 0.0392 | 0.0076 | 0.0014 | 0.0822 | 0.0762 | 0.081 | 0.0717 | 0.0034 | 0.0036 | 0.1249 | 0.0192 | 0.017 | 0.0017 | 0 | 0.0004 | 0.0415 |
Nucleus | 0.8879 | 0.5694 | 0.2772 | 0.1768 | 0.0121 | 0.7444 | 0.7748 | 0.5419 | 0.3025 | 0.0576 | 0.031 | 0.7091 | 0.8646 | 0.6267 | 0.1045 | 0.0007 | 0.0105 | 0.7577 |
Peroxisomes | 0.002 | 0.0011 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0105 | 0.0029 | 0.0012 | 0.0098 | 0.0144 | 0.0004 | 0.0231 | 0.0002 | 0.0048 | 0 | 0.0001 | 0.0065 |
Punctate Nuclear | 0.0062 | 0.0222 | 0.0308 | 0.0031 | 0.0004 | 0.0072 | 0.0084 | 0.0321 | 0.0525 | 0.0059 | 0.0066 | 0.018 | 0.0119 | 0.091 | 0.0042 | 0.0001 | 0.0005 | 0.0186 |
Vacuole | 0.0005 | 0.0038 | 0.0247 | 0.045 | 0.0187 | 0.0039 | 0.0074 | 0.0153 | 0.0225 | 0.0251 | 0.0173 | 0.0035 | 0.0003 | 0.0008 | 0.0279 | 0.0036 | 0.0223 | 0.0007 |
Vacuole Periphery | 0.0003 | 0.0007 | 0.003 | 0.0045 | 0.0029 | 0.0003 | 0.0024 | 0.0013 | 0.0035 | 0.0054 | 0.0039 | 0.0007 | 0.0004 | 0.0003 | 0.0047 | 0.0008 | 0.0023 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.7182 | 6.4321 | 37.6214 | 40.2114 | 22.2247 | 24.5347 | 14.5371 | 24.1417 | 35.514 | 26.9798 |
Translational Efficiency | 1.3012 | 0.9666 | 1.1213 | 1.2639 | 1.1237 | 1.138 | 0.9387 | 0.9721 | 1.0172 | 0.9913 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1092 | 1689 | 181 | 1206 | 1798 | 1769 | 2764 | 1900 | 2890 | 3458 | 2945 | 3106 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 916.76 | 1008.97 | 1252.07 | 1300.85 | 833.71 | 971.63 | 1152.86 | 1256.63 | 865.09 | 989.87 | 1158.96 | 1273.80 |
Standard Deviation | 146.07 | 194.85 | 186.58 | 260.30 | 151.46 | 161.00 | 187.90 | 221.85 | 154.78 | 179.31 | 189.33 | 238.50 |
Intensity Change Log 2 | — | 0.138267 | 0.449699 | 0.504839 | — | 0.220861 | 0.467600 | 0.591942 | — | 0.178195 | 0.458253 | 0.546981 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000504 | 0.000889 | 0.001554 | 0.000865 | 0.000696 | 0.000824 | 0.000916 | 0.000562 | 0.000623 | 0.000856 | 0.000955 | 0.000679 |
Bud Neck | 0.031981 | 0.028435 | 0.019135 | 0.019513 | 0.023484 | 0.031195 | 0.065395 | 0.028353 | 0.026695 | 0.029847 | 0.062552 | 0.024921 |
Bud Site | 0.010525 | 0.011150 | 0.006854 | 0.031350 | 0.012191 | 0.011540 | 0.014480 | 0.022982 | 0.011562 | 0.011350 | 0.014011 | 0.026231 |
Cell Periphery | 0.000799 | 0.000715 | 0.000180 | 0.000702 | 0.001105 | 0.000492 | 0.000324 | 0.000314 | 0.000989 | 0.000601 | 0.000315 | 0.000465 |
Cytoplasm | 0.081407 | 0.093425 | 0.067431 | 0.595400* | 0.141702* | 0.079820 | 0.325982* | 0.547053* | 0.118919* | 0.086465 | 0.310092* | 0.565825* |
Cytoplasmic Foci | 0.007432 | 0.009117 | 0.016592 | 0.023149 | 0.013677 | 0.009471 | 0.009038 | 0.019392 | 0.011317 | 0.009298 | 0.009502 | 0.020851 |
Eisosomes | 0.000052 | 0.000044 | 0.000059 | 0.000018 | 0.000044 | 0.000054 | 0.000020 | 0.000012 | 0.000047 | 0.000049 | 0.000023 | 0.000015 |
Endoplasmic Reticulum | 0.003302 | 0.002857 | 0.002244 | 0.001020 | 0.004192 | 0.003109 | 0.000870 | 0.000914 | 0.003855 | 0.002986 | 0.000955 | 0.000955 |
Endosome | 0.003517 | 0.003515 | 0.003345 | 0.004747 | 0.006986 | 0.004976 | 0.000837 | 0.002046 | 0.005675 | 0.004262 | 0.000991 | 0.003094 |
Golgi | 0.000357 | 0.001014 | 0.000424 | 0.004737 | 0.001097 | 0.001279 | 0.000272 | 0.000979 | 0.000817 | 0.001150 | 0.000282 | 0.002438 |
Lipid Particles | 0.000387 | 0.000894 | 0.000813 | 0.001854 | 0.000804 | 0.000540 | 0.000252 | 0.000362 | 0.000646 | 0.000713 | 0.000286 | 0.000941 |
Mitochondria | 0.001168 | 0.002123 | 0.002301 | 0.003280 | 0.001999 | 0.002195 | 0.001832 | 0.001717 | 0.001685 | 0.002160 | 0.001861 | 0.002324 |
Mitotic Spindle | 0.005439 | 0.010162 | 0.020471 | 0.082238 | 0.006993 | 0.020945 | 0.047144 | 0.048693 | 0.006406 | 0.015678 | 0.045505 | 0.061718 |
None | 0.006855 | 0.007107 | 0.001312 | 0.007631 | 0.022096 | 0.006251 | 0.009397 | 0.007991 | 0.016337 | 0.006669 | 0.008900 | 0.007851 |
Nuclear Periphery | 0.000658 | 0.001389 | 0.000652 | 0.001267 | 0.000757 | 0.000917 | 0.000976 | 0.000933 | 0.000720 | 0.001148 | 0.000957 | 0.001063 |
Nuclear Periphery Foci | 0.004419 | 0.008733 | 0.006016 | 0.002880 | 0.008221 | 0.003613 | 0.000766 | 0.001071 | 0.006784 | 0.006114 | 0.001089 | 0.001773 |
Nucleolus | 0.141550 | 0.181106 | 0.123867 | 0.008201 | 0.117914 | 0.148104 | 0.030493 | 0.011989 | 0.126845 | 0.164223 | 0.036232 | 0.010518 |
Nucleus | 0.690214* | 0.628347* | 0.703818* | 0.172446* | 0.612984* | 0.663539* | 0.477036* | 0.273242* | 0.642165* | 0.646350* | 0.490974* | 0.234105* |
Peroxisomes | 0.000646 | 0.001389 | 0.003409 | 0.000790 | 0.000892 | 0.001625 | 0.000388 | 0.000634 | 0.000799 | 0.001510 | 0.000573 | 0.000695 |
Vacuole | 0.007255 | 0.005773 | 0.019034 | 0.036456 | 0.018240 | 0.007418 | 0.012897 | 0.028988 | 0.014089 | 0.006615 | 0.013274 | 0.031888 |
Vacuole Periphery | 0.001535 | 0.001816 | 0.000491 | 0.001456 | 0.003927 | 0.002091 | 0.000685 | 0.001773 | 0.003023 | 0.001957 | 0.000673 | 0.001650 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.42 | -8.98 | -7.42 | -3.18 | 3.49 | -2.23 | -0.44 | 0.16 | 1.56 | 0.48 | -6.00 | -1.12 | -3.85 | -0.29 | -0.16 |
Bud Neck | 0.68 | -0.51 | -2.92 | -3.90 | -1.27 | -4.58 | -22.47 | -9.58 | -5.66 | 11.87 | -3.02 | -21.63 | -9.37 | -7.04 | 13.44 |
Bud Site | -1.04 | 0.10 | -9.31 | -8.97 | -8.07 | 0.20 | -3.40 | -5.61 | -5.55 | -3.25 | -0.44 | -4.25 | -10.45 | -10.14 | -7.15 |
Cell Periphery | 0.48 | 4.34 | 1.73 | 1.35 | -4.33 | 5.81 | 8.34 | 8.18 | 4.82 | -0.27 | 4.37 | 9.59 | 7.63 | 3.95 | -4.76 |
Cytoplasm | -1.48 | 0.59 | -23.81 | -23.66 | -18.12 | 7.55 | -12.80 | -24.60 | -32.26 | -14.04 | 5.32 | -16.49 | -33.77 | -39.87 | -16.90 |
Cytoplasmic Foci | -0.82 | -1.56 | -5.60 | -5.13 | -1.28 | 2.56 | 4.43 | -0.38 | -3.15 | -5.54 | 1.84 | 3.20 | -3.47 | -5.63 | -7.64 |
Eisosomes | 2.37 | -2.20 | 7.47 | 6.37 | 7.88 | -3.51 | 11.75 | 14.26 | 17.56 | 5.29 | -1.09 | 14.12 | 15.16 | 17.66 | 3.09 |
Endoplasmic Reticulum | 1.89 | 1.45 | 5.03 | 4.00 | 2.04 | 3.25 | 12.54 | 10.87 | 7.18 | -2.62 | 4.07 | 14.90 | 11.84 | 8.64 | -4.33 |
Endosome | -0.52 | 0.73 | 1.20 | 1.57 | -0.03 | 2.14 | 9.57 | 8.28 | 4.57 | -4.51 | 2.20 | 10.95 | 7.80 | 4.96 | -4.89 |
Golgi | -3.45 | -0.56 | -2.29 | -1.45 | -2.21 | -0.38 | 9.95 | 2.62 | 1.95 | -2.07 | -1.73 | 9.61 | -1.26 | -0.38 | -2.84 |
Lipid Particles | -1.55 | -1.52 | -5.68 | -2.34 | -3.11 | 1.52 | 3.87 | 2.03 | -0.06 | -3.29 | -0.33 | 3.24 | -3.08 | -1.69 | -6.47 |
Mitochondria | -6.61 | -6.03 | -3.83 | -2.05 | -0.65 | -0.87 | -2.11 | -1.29 | -0.38 | 0.45 | -3.20 | -5.65 | -3.87 | -1.97 | -1.14 |
Mitotic Spindle | -2.55 | -2.16 | -15.66 | -14.87 | -7.75 | -5.27 | -16.78 | -14.24 | -10.00 | -0.59 | -5.68 | -17.33 | -21.03 | -17.79 | -4.62 |
None | -0.14 | 6.38 | -1.14 | -1.30 | -10.65 | 9.97 | 9.03 | 9.34 | -2.43 | 1.50 | 9.32 | 7.95 | 8.16 | -2.74 | 0.86 |
Nuclear Periphery | -2.57 | -2.05 | -8.35 | -3.29 | -4.92 | -1.43 | -7.49 | -6.65 | -5.19 | -0.00 | -2.84 | -8.62 | -10.55 | -4.93 | -2.31 |
Nuclear Periphery Foci | -1.80 | -0.28 | 1.01 | 3.09 | 0.70 | 3.04 | 6.42 | 5.69 | 2.84 | -1.56 | 0.70 | 6.61 | 5.17 | 4.42 | -2.76 |
Nucleolus | -5.55 | 1.92 | 22.39* | 31.15* | 7.97 | -5.18 | 17.86 | 23.87* | 27.68* | 8.40 | -8.26 | 21.72 | 32.62* | 41.44* | 11.54 |
Nucleus | 5.92 | 0.56 | 28.98 | 25.83 | 14.64 | -4.45 | 12.68 | 22.74 | 27.85 | 12.31 | -0.03 | 16.97 | 35.25 | 37.57 | 17.79 |
Peroxisomes | -5.40 | -6.32 | -1.98 | 3.48 | 5.59 | -3.18 | 5.07 | 4.06 | 5.35 | -1.08 | -5.26 | 2.88 | 2.06 | 6.74 | -0.87 |
Vacuole | 1.30 | -2.88 | -11.85 | -12.64 | -2.84 | 5.65 | -0.51 | -7.11 | -12.80 | -7.28 | 6.06 | -3.43 | -12.76 | -18.21 | -9.62 |
Vacuole Periphery | -1.08 | 5.49 | 2.65 | 3.04 | -1.27 | 3.14 | 6.63 | 4.62 | 2.12 | -1.58 | 2.82 | 7.59 | 5.14 | 3.16 | -1.95 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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