Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 3 | 1 |
Bud | 5 | 1 | 23 | 21 | 18 | 12 | – | 28 | 38 | 29 | 31 | 42 | 6 | 2 | 0 | 1 | 2 | 4 | 4 | 2 | 0 | 5 | 8 | 12 |
Bud Neck | 14 | 6 | 41 | 19 | 24 | 3 | – | 23 | 10 | 17 | 20 | 19 | 32 | 25 | 22 | 9 | 22 | 15 | 6 | 8 | 4 | 2 | 11 | 16 |
Bud Site | 8 | 0 | 15 | 31 | 16 | 21 | – | 36 | 42 | 30 | 34 | 58 | 1 | 0 | 0 | 0 | 2 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 17 | 23 | 42 | 30 | 40 | 39 | – | 30 | 26 | 31 | 21 | 62 | 115 | 137 | 228 | 18 | 25 | 26 | 4 | 15 | 11 | 8 | 10 | 14 |
Endoplasmic Reticulum | 1 | 4 | 0 | 2 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 5 | 4 | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 42 | 106 | 1 | 10 | 21 | 11 | – | 19 | 10 | 15 | 6 | 5 | 239 | 174 | 214 | 79 | 37 | 31 | 86 | 58 | 54 | 58 | 112 | 121 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 0 | 1 | 2 | 3 | 3 |
Mitochondria | 106 | 26 | 132 | 110 | 33 | 150 | – | 184 | 219 | 167 | 218 | 268 | 9 | 4 | 2 | 9 | 33 | 14 | 8 | 7 | 15 | 6 | 11 | 17 |
Nucleus | 59 | 31 | 22 | 16 | 12 | 17 | – | 28 | 30 | 23 | 35 | 23 | 31 | 34 | 45 | 19 | 30 | 20 | 12 | 11 | 7 | 5 | 7 | 7 |
Nuclear Periphery | 3 | 3 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 6 |
Nucleolus | 124 | 79 | 39 | 66 | 62 | 92 | – | 126 | 119 | 88 | 86 | 78 | 178 | 112 | 161 | 87 | 89 | 57 | 138 | 98 | 58 | 53 | 88 | 91 |
Peroxisomes | 1 | 7 | 4 | 68 | 22 | 35 | – | 43 | 38 | 26 | 40 | 57 | 30 | 30 | 38 | 5 | 4 | 2 | 2 | 0 | 2 | 1 | 3 | 5 |
SpindlePole | 336 | 191 | 178 | 253 | 262 | 227 | – | 367 | 273 | 254 | 258 | 272 | 423 | 323 | 454 | 212 | 212 | 160 | 322 | 206 | 189 | 246 | 379 | 337 |
Vac/Vac Membrane | 2 | 14 | 2 | 1 | 1 | 2 | – | 3 | 2 | 2 | 1 | 5 | 5 | 7 | 4 | 3 | 6 | 3 | 6 | 13 | 6 | 7 | 12 | 21 |
Unique Cell Count | 501 | 354 | 345 | 405 | 374 | 399 | 580 | 477 | 438 | 460 | 566 | 724 | 611 | 829 | 280 | 290 | 220 | 611 | 450 | 381 | 410 | 676 | 681 | |
Labelled Cell Count | 718 | 491 | 499 | 628 | 513 | 609 | 889 | 807 | 683 | 752 | 890 | 1073 | 850 | 1171 | 445 | 469 | 339 | 611 | 450 | 381 | 410 | 676 | 681 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 5.2 | 3.3 | 3.3 | 3.9 | 3.1 | – | 3.4 | 2.9 | 3.1 | 2.9 | 3.0 | 4.8 | 5.3 | 5.1 | 6.3 | 6.4 | 6.3 | 4.9 | 5.1 | 5.0 |
Std Deviation (1e-4) | 0.6 | 1.2 | 0.9 | 2.0 | 1.5 | 1.2 | – | 1.3 | 1.2 | 1.2 | 1.1 | 3.0 | 1.1 | 1.6 | 1.5 | 1.5 | 1.6 | 1.7 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.24 | -0.09 | – | 0.05 | -0.2 | -0.07 | -0.16 | -0.15 | 0.55 | 0.68 | 0.63 | 0.94 | 0.95 | 0.94 | 0.58 | 0.64 | 0.61 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -5.9 | -7.5 | -4.1 | -3.9 | -2.6 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -4.6 | -6.4 | -4.0 | -1.8 | -2.0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -5.2 | 0 | -3.5 | -2.8 | -2.7 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.2 | -0.6 | -1.0 | – | -3.8 | -3.5 | -2.4 | -4.0 | -0.6 | 1.6 | 3.9 | 5.7 | -2.4 | -1.5 | -0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 10.8 | 10.3 | 10.4 | 6.6 | 6.9 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -16.0 | -18.7 | -10.4 | -7.7 | -8.4 |
Nucleus | -1.5 | -2.0 | -1.3 | – | -1.0 | -0.1 | -0.7 | 0.7 | -1.6 | -1.5 | -0.5 | -0.6 | 0.2 | 1.8 | 1.2 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 3.4 | 6.1 | 6.1 | 4.5 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 2.9 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 1.0 | 6.2 | 5.5 | 5.0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8295 | 0.966 | 0.7569 | 0.749 | 1.5703 | 0.8226 | 0.3714 | 0.0868 | 0.1012 | 0.0788 | 0.2629 | 0.1502 | 2.1768 | 2.3368 | 2.0927 | 2.4667 | 2.5354 | 2.205 |
Actin | 0.0018 | 0.0001 | 0.0003 | 0 | 0.0066 | 0.0004 | 0.0214 | 0.0002 | 0.0019 | 0.0029 | 0.0014 | 0.0043 | 0.001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0021 | 0.0002 | 0.0012 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0033 | 0.0056 | 0.0065 | 0.0076 | 0.0032 | 0.0035 | 0.0141 | 0.0048 | 0.014 | 0.0098 | 0.0048 | 0.0025 | 0.0016 | 0.0028 | 0.0024 | 0.007 | 0.0018 | 0.0018 |
Bud Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0012 | 0.0002 | 0.0031 | 0.0004 | 0.0022 | 0.0006 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0018 | 0.0041 | 0.0024 | 0.0015 | 0.0126 | 0.0008 | 0.0139 | 0.0067 | 0.0092 | 0.0018 | 0.0052 | 0.001 | 0.0005 | 0.0025 | 0.0006 | 0.0004 | 0.0006 | 0.0002 |
Cell Periphery | 0.0004 | 0.0003 | 0.0003 | 0.0016 | 0.0003 | 0.0001 | 0.001 | 0.0006 | 0.0008 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.0016 | 0.0049 | 0.0012 | 0.0001 | 0.0075 | 0.0008 | 0.0087 | 0.0036 | 0.003 | 0.0002 | 0.0189 | 0.0006 | 0.0014 | 0.0008 | 0.0001 | 0.0001 | 0.0005 | 0.0001 |
Cytoplasmic Foci | 0.0569 | 0.0291 | 0.0388 | 0.0101 | 0.0275 | 0.0222 | 0.0757 | 0.025 | 0.0319 | 0.0163 | 0.027 | 0.0119 | 0.0129 | 0.0064 | 0.011 | 0.0037 | 0.0027 | 0.0037 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0026 | 0.0005 | 0.002 | 0.0004 | 0.0008 | 0.0019 | 0.0015 | 0.0004 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 |
Endosome | 0.0201 | 0.0087 | 0.0217 | 0.006 | 0.0291 | 0.0136 | 0.0363 | 0.0162 | 0.0179 | 0.0135 | 0.0196 | 0.0119 | 0.0046 | 0.002 | 0.0019 | 0.0007 | 0.0003 | 0.0008 |
Golgi | 0.0076 | 0.0026 | 0.0105 | 0.0016 | 0.03 | 0.004 | 0.0134 | 0.0037 | 0.0047 | 0.0107 | 0.0082 | 0.0075 | 0.0016 | 0.001 | 0.0012 | 0.0005 | 0.0003 | 0.0005 |
Lipid Particles | 0.1679 | 0.0802 | 0.1695 | 0.0437 | 0.0411 | 0.0766 | 0.1539 | 0.1076 | 0.128 | 0.1155 | 0.0628 | 0.0515 | 0.0481 | 0.0303 | 0.0407 | 0.011 | 0.0049 | 0.0118 |
Mitochondria | 0.0022 | 0.0003 | 0.0014 | 0.0002 | 0.0814 | 0.0123 | 0.0083 | 0.0006 | 0.0036 | 0.0147 | 0.0111 | 0.0051 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
None | 0.0043 | 0.0026 | 0.007 | 0.0001 | 0.0165 | 0.0003 | 0.0106 | 0.019 | 0.0068 | 0.0004 | 0.0013 | 0.0011 | 0.001 | 0.0086 | 0.0001 | 0.0004 | 0.01 | 0.0001 |
Nuclear Periphery | 0.0015 | 0.0097 | 0.0039 | 0.0004 | 0.0326 | 0.0056 | 0.0056 | 0.0073 | 0.0071 | 0.0124 | 0.0136 | 0.0023 | 0.0043 | 0.0081 | 0.0023 | 0.0004 | 0.0013 | 0.0054 |
Nucleolus | 0.3289 | 0.4114 | 0.4626 | 0.485 | 0.3167 | 0.2663 | 0.1986 | 0.3936 | 0.4116 | 0.4945 | 0.2021 | 0.2557 | 0.357 | 0.4459 | 0.4867 | 0.5028 | 0.3373 | 0.2727 |
Nucleus | 0.0245 | 0.0558 | 0.036 | 0.0284 | 0.0322 | 0.0208 | 0.0333 | 0.0666 | 0.0551 | 0.0228 | 0.0969 | 0.0272 | 0.0493 | 0.0959 | 0.0499 | 0.0696 | 0.0569 | 0.035 |
Peroxisomes | 0.0161 | 0.0032 | 0.0061 | 0.0013 | 0.0086 | 0.0063 | 0.0163 | 0.0069 | 0.0057 | 0.0031 | 0.0055 | 0.0053 | 0.0024 | 0.0013 | 0.0013 | 0.0006 | 0.0007 | 0.0005 |
Punctate Nuclear | 0.3559 | 0.3754 | 0.227 | 0.3731 | 0.3414 | 0.5638 | 0.3712 | 0.318 | 0.2717 | 0.2718 | 0.5107 | 0.6085 | 0.5121 | 0.3908 | 0.4004 | 0.4013 | 0.5819 | 0.6671 |
Vacuole | 0.0038 | 0.0042 | 0.0033 | 0.0378 | 0.0037 | 0.0014 | 0.0069 | 0.0172 | 0.0197 | 0.0033 | 0.0059 | 0.002 | 0.0009 | 0.0025 | 0.0008 | 0.0011 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0006 | 0.0009 | 0.0008 | 0.0007 | 0.0043 | 0.0005 | 0.0031 | 0.0012 | 0.0031 | 0.003 | 0.0014 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0768 | 9.008 | 15.0397 | 7.3556 | 8.1656 | 10.0712 | 10.8157 | 12.2945 | 8.0221 | 9.3753 |
Translational Efficiency | 2.104 | 2.1328 | 0.93 | 1.8692 | 1.7277 | 2.4034 | 1.7709 | 1.7488 | 2.0569 | 2.1356 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2080 | 1734 | 216 | 1090 | 1560 | 1046 | 1361 | 360 | 3640 | 2780 | 1577 | 1450 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 658.96 | 724.76 | 921.14 | 799.21 | 526.62 | 728.95 | 867.53 | 907.69 | 602.24 | 726.34 | 874.87 | 826.14 |
Standard Deviation | 77.07 | 89.04 | 119.87 | 121.87 | 64.59 | 91.28 | 102.57 | 112.11 | 97.32 | 89.91 | 106.71 | 128.38 |
Intensity Change Log 2 | — | 0.137312 | 0.483230 | 0.278384 | — | 0.469058 | 0.720151 | 0.785437 | — | 0.294146 | 0.593294 | 0.525786 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000090 | 0.000283 | 0.000904 | 0.000945 | 0.000146 | 0.000353 | 0.000316 | 0.000695 | 0.000114 | 0.000310 | 0.000397 | 0.000883 |
Bud Neck | 0.012216 | 0.013213 | 0.009314 | 0.022668 | 0.011036 | 0.013738 | 0.006955 | 0.026869 | 0.011710 | 0.013410 | 0.007278 | 0.023711 |
Bud Site | 0.002174 | 0.003853 | 0.003411 | 0.029100 | 0.002501 | 0.003333 | 0.002077 | 0.017136 | 0.002314 | 0.003658 | 0.002260 | 0.026130 |
Cell Periphery | 0.000121 | 0.000122 | 0.000087 | 0.000300 | 0.000289 | 0.000098 | 0.000036 | 0.000057 | 0.000193 | 0.000113 | 0.000043 | 0.000239 |
Cytoplasm | 0.124662* | 0.041954 | 0.006009 | 0.158266* | 0.144715* | 0.048808 | 0.002197 | 0.025815 | 0.133256* | 0.044533 | 0.002719 | 0.125382* |
Cytoplasmic Foci | 0.191815* | 0.107112 | 0.002355 | 0.023203 | 0.263899* | 0.108929 | 0.000413 | 0.007535 | 0.222708* | 0.107796 | 0.000679 | 0.019313 |
Eisosomes | 0.000118 | 0.000113 | 0.000107 | 0.000100 | 0.000186 | 0.000120 | 0.000055 | 0.000046 | 0.000147 | 0.000116 | 0.000063 | 0.000087 |
Endoplasmic Reticulum | 0.000729 | 0.001403 | 0.004274 | 0.003248 | 0.001257 | 0.001056 | 0.001629 | 0.002225 | 0.000955 | 0.001272 | 0.001991 | 0.002994 |
Endosome | 0.031838 | 0.042847 | 0.002503 | 0.027100 | 0.015209 | 0.036485 | 0.001693 | 0.017279 | 0.024711 | 0.040453 | 0.001804 | 0.024661 |
Golgi | 0.006459 | 0.016058 | 0.001991 | 0.006027 | 0.010699 | 0.010023 | 0.001086 | 0.001567 | 0.008276 | 0.013787 | 0.001210 | 0.004919 |
Lipid Particles | 0.107515* | 0.060883 | 0.001503 | 0.004577 | 0.161813* | 0.060474 | 0.000385 | 0.005215 | 0.130786* | 0.060729 | 0.000538 | 0.004736 |
Mitochondria | 0.013543 | 0.019023 | 0.000659 | 0.010534 | 0.041337 | 0.024441 | 0.000736 | 0.003599 | 0.025455 | 0.021062 | 0.000726 | 0.008812 |
Mitotic Spindle | 0.078998 | 0.279642* | 0.268442* | 0.268421* | 0.021685 | 0.194225* | 0.340571* | 0.665266* | 0.054435 | 0.247503* | 0.330692* | 0.366948* |
None | 0.020204 | 0.011797 | 0.001866 | 0.013217 | 0.042862 | 0.010007 | 0.000991 | 0.002078 | 0.029915 | 0.011124 | 0.001111 | 0.010451 |
Nuclear Periphery | 0.004656 | 0.003520 | 0.001449 | 0.002540 | 0.002891 | 0.003343 | 0.001602 | 0.001608 | 0.003900 | 0.003454 | 0.001581 | 0.002308 |
Nuclear Periphery Foci | 0.056379 | 0.096646 | 0.071284 | 0.009013 | 0.018225 | 0.060504 | 0.039921 | 0.019555 | 0.040027 | 0.083047 | 0.044216 | 0.011630 |
Nucleolus | 0.249703* | 0.216085 | 0.423063* | 0.117207 | 0.101738 | 0.312712* | 0.460623* | 0.106097 | 0.186289 | 0.252442* | 0.455478* | 0.114449 |
Nucleus | 0.075410 | 0.053552 | 0.190157* | 0.224046* | 0.130719* | 0.078310 | 0.134528* | 0.050537 | 0.099114 | 0.062867 | 0.142148* | 0.180968* |
Peroxisomes | 0.017272 | 0.024447 | 0.006929 | 0.006872 | 0.021842 | 0.024296 | 0.002598 | 0.004324 | 0.019231 | 0.024390 | 0.003191 | 0.006239 |
Vacuole | 0.005366 | 0.005806 | 0.003247 | 0.066805 | 0.005562 | 0.006019 | 0.001235 | 0.041896 | 0.005450 | 0.005886 | 0.001510 | 0.060621 |
Vacuole Periphery | 0.000732 | 0.001643 | 0.000443 | 0.005811 | 0.001390 | 0.002728 | 0.000353 | 0.000602 | 0.001014 | 0.002051 | 0.000365 | 0.004518 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.66 | -6.30 | -11.60 | -9.08 | 2.57 | -1.92 | -10.09 | -7.49 | -5.37 | -4.69 | -5.28 | -11.59 | -13.56 | -10.17 | -4.89 |
Bud Neck | -0.33 | -2.58 | -10.70 | -10.13 | -6.64 | -1.71 | -1.34 | -9.33 | -8.60 | -8.99 | -1.32 | -2.16 | -14.04 | -13.05 | -12.60 |
Bud Site | -3.31 | -6.53 | -12.27 | -11.53 | -8.77 | -0.98 | -4.61 | -9.39 | -9.20 | -8.82 | -3.06 | -7.55 | -15.28 | -14.63 | -13.59 |
Cell Periphery | -0.67 | -1.79 | -5.19 | -4.71 | -3.32 | 4.96 | 6.39 | 4.58 | -1.30 | -7.50 | 4.39 | 7.65 | -0.27 | -5.08 | -8.63 |
Cytoplasm | 11.10 | 17.62 | -4.10 | -11.85 | -16.12 | 14.01 | 25.26 | 11.20 | 0.18 | -6.39 | 17.78 | 31.90 | 0.60 | -11.00 | -18.20 |
Cytoplasmic Foci | 14.38 | 40.16 | 36.59 | 22.24 | -9.54 | 20.16 | 44.52 | 41.43 | 18.46 | -5.78 | 24.38 | 59.33 | 54.11 | 28.67 | -13.13 |
Eisosomes | -1.39 | -2.59 | -0.67 | 0.21 | 2.02 | 9.38 | 21.33 | 8.75 | 2.63 | -4.30 | 6.46 | 19.53 | 5.67 | 1.36 | -7.74 |
Endoplasmic Reticulum | -10.35 | -8.97 | -15.23 | -11.57 | 3.27 | 0.56 | -7.68 | -6.58 | -6.70 | -4.96 | -5.80 | -14.14 | -15.02 | -13.24 | -6.41 |
Endosome | -3.57 | 18.58 | 5.24 | 7.74 | -9.30 | -8.22 | 12.00 | -2.11 | 4.66 | -7.90 | -7.88 | 23.07 | 2.62 | 8.96 | -12.60 |
Golgi | -7.50 | 7.65 | 5.05 | 9.93 | -0.10 | 1.34 | 11.90 | 11.09 | 9.93 | -2.04 | -5.06 | 17.03 | 9.42 | 12.14 | -2.79 |
Lipid Particles | 8.12 | 23.67 | 23.22 | 16.21 | -4.10 | 16.38 | 29.33 | 28.62 | 13.11 | -3.67 | 17.34 | 37.23 | 36.24 | 20.87 | -9.76 |
Mitochondria | -2.24 | 17.15 | 5.40 | 5.98 | -4.91 | 5.88 | 15.15 | 14.33 | 9.84 | -1.81 | 4.10 | 19.51 | 13.64 | 9.81 | -5.17 |
Mitotic Spindle | -20.89* | -9.14 | -18.79 | -2.89 | -0.54 | -17.91* | -33.08* | -23.03 | -13.60 | -6.17 | -27.78 | -30.85 | -28.61 | -9.83 | -0.62 |
None | 6.00 | 15.81 | 6.96 | 2.06 | -5.21 | 11.46 | 16.60 | 14.72 | 6.01 | -5.61 | 12.77 | 22.55 | 14.28 | 3.69 | -6.89 |
Nuclear Periphery | 2.91 | 8.46 | 2.08 | -0.90 | -6.79 | -1.02 | 3.50 | -2.03 | -1.56 | -4.10 | 1.57 | 8.77 | -0.18 | -1.65 | -8.12 |
Nuclear Periphery Foci | -6.94 | -0.61 | 17.09 | 20.42* | 7.42 | -8.23 | -5.18 | -1.07 | 4.94 | 2.05 | -10.55 | 0.92 | 13.19 | 20.62 | 10.10 |
Nucleolus | 5.63 | -3.79 | 16.13 | 10.83 | 12.17 | -18.41* | -31.16* | 1.32 | 15.45 | 24.02* | -7.22 | -22.44 | 12.15 | 17.81 | 30.55* |
Nucleus | 4.10 | -8.98 | -17.56 | -19.21 | -2.05 | 7.94 | -0.64 | 1.16 | -3.50 | 1.59 | 9.15 | -9.51 | -14.02 | -18.47 | -7.43 |
Peroxisomes | -3.92 | 9.01 | 9.17 | 11.54 | 1.35 | 0.23 | 22.69 | 13.01 | 9.31 | -2.96 | -2.64 | 26.72 | 14.58 | 14.38 | -3.92 |
Vacuole | -1.11 | -2.27 | -14.92 | -14.78 | -13.89 | -0.02 | 1.49 | -8.16 | -8.15 | -8.31 | -0.73 | 0.50 | -16.97 | -16.88 | -16.97 |
Vacuole Periphery | -3.64 | -1.21 | -4.83 | -3.44 | -4.69 | -1.18 | 6.89 | 2.79 | 1.75 | -6.10 | -2.39 | 6.63 | -4.24 | -2.14 | -5.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|