Standard name | |
---|---|
Human Ortholog | |
Description | Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 2 | 1 | 0 | 3 | 12 | 25 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 |
Bud | 0 | 0 | 5 | 0 | 0 | 1 | – | 1 | 1 | 7 | 3 | 5 | 0 | 3 | 8 | 0 | 0 | 0 | 9 | 4 | 2 | 1 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 4 | 4 | – | 12 | 5 | 11 | 5 | 6 | 2 | 1 | 2 | 0 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 174 | 128 | 82 | 70 | 95 | 128 | – | 118 | 105 | 100 | 97 | 97 | 93 | 120 | 126 | 108 | 139 | 172 | 126 | 96 | 105 | 56 | 128 | 152 |
Endoplasmic Reticulum | 1 | 2 | 4 | 5 | 4 | 5 | – | 15 | 6 | 2 | 8 | 10 | 2 | 6 | 11 | 5 | 5 | 2 | 1 | 1 | 2 | 3 | 4 | 3 |
Endosome | 0 | 0 | 0 | 1 | 4 | 1 | – | 0 | 1 | 1 | 0 | 0 | 6 | 6 | 6 | 0 | 0 | 0 | 3 | 1 | 2 | 2 | 1 | 2 |
Golgi | 0 | 1 | 0 | 0 | 2 | 0 | – | 2 | 1 | 1 | 0 | 0 | 7 | 12 | 37 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 2 | 3 | 13 | 20 | 12 | 45 | – | 61 | 78 | 83 | 65 | 88 | 1 | 3 | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 5 | 2 | 2 |
Nucleus | 22 | 11 | 12 | 3 | 1 | 1 | – | 13 | 10 | 14 | 25 | 28 | 1 | 4 | 1 | 0 | 0 | 3 | 137 | 66 | 34 | 21 | 35 | 53 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 1 | 0 | 4 | 2 | – | 2 | 5 | 1 | 0 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 132 | 48 | 58 | 30 | 71 | 61 | – | 134 | 56 | 67 | 46 | 43 | 130 | 66 | 87 | 25 | 42 | 62 | 21 | 19 | 10 | 14 | 25 | 28 |
Unique Cell Count | 282 | 169 | 154 | 112 | 172 | 228 | 311 | 235 | 243 | 220 | 250 | 218 | 205 | 260 | 128 | 174 | 229 | 313 | 198 | 177 | 113 | 214 | 266 | |
Labelled Cell Count | 331 | 193 | 175 | 130 | 197 | 249 | 361 | 270 | 290 | 250 | 278 | 247 | 237 | 309 | 140 | 193 | 249 | 313 | 198 | 177 | 113 | 214 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.9 | 16.3 | 9.4 | 12.1 | 12.9 | 11.5 | – | 11.5 | 10.8 | 11.4 | 11.6 | 11.2 | 14.6 | 14.5 | 14.7 | 18.9 | 19.8 | 19.5 | 13.6 | 15.9 | 16.2 |
Std Deviation (1e-4) | 2.8 | 3.0 | 2.4 | 2.9 | 2.8 | 2.5 | – | 2.7 | 3.1 | 2.9 | 3.2 | 2.8 | 3.1 | 2.6 | 2.7 | 6.2 | 5.7 | 5.6 | 3.5 | 3.1 | 3.4 |
Intensity Change (Log2) | – | – | – | 0.36 | 0.45 | 0.29 | – | 0.29 | 0.19 | 0.27 | 0.29 | 0.24 | 0.63 | 0.61 | 0.64 | 1.0 | 1.07 | 1.04 | 0.53 | 0.75 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 4.0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | 0.4 | 0.6 | – | -3.1 | -1.7 | -2.4 | -1.7 | -2.8 | -2.0 | 1.0 | -0.9 | 5.6 | 5.1 | 4.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 1.1 | 0 | 0 | 0 | 0.7 | 0 | 0 | 0.9 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 4.9 | 0 | 0 | 0 |
Mitochondria | 2.3 | -0.5 | 3.0 | – | 3.1 | 5.6 | 5.8 | 4.9 | 6.0 | -4.0 | -3.2 | 0 | -2.9 | -3.2 | -4.1 |
Nucleus | -1.8 | -3.3 | -3.9 | – | -1.6 | -1.5 | -0.8 | 1.1 | 1.1 | -3.8 | -2.7 | 0 | -3.2 | -3.8 | -3.2 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | 0.7 | -2.3 | – | 1.1 | -2.9 | -2.1 | -3.6 | -4.6 | 4.2 | -1.1 | -0.9 | -3.3 | -2.7 | -2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.2271 | 9.0697 | 8.024 | 7.8038 | 5.7876 | 7.7083 | 6.3655 | 8.7097 | 7.1507 | 6.5332 | 5.7903 | 7.6385 | 7.8377 | 10.3292 | 9.4229 | 8.0614 | 9.7947 | 9.4161 |
Actin | 0.0195 | 0.0013 | 0.0009 | 0.0004 | 0.0293 | 0.0045 | 0.0244 | 0.0013 | 0.0142 | 0.0006 | 0.0012 | 0.0008 | 0.0192 | 0.0024 | 0.0279 | 0.0037 | 0.0018 | 0.0049 |
Bud | 0.0019 | 0.0002 | 0.0004 | 0.0002 | 0.0008 | 0.0005 | 0.0016 | 0.0005 | 0.0011 | 0.0002 | 0.0007 | 0.0003 | 0.0013 | 0.0003 | 0.0007 | 0.0003 | 0.0001 | 0.0003 |
Bud Neck | 0.0012 | 0.0004 | 0.0006 | 0.0008 | 0.0026 | 0.0024 | 0.0011 | 0.0003 | 0.0009 | 0.0011 | 0.0006 | 0.002 | 0.0012 | 0.0005 | 0.0007 | 0.0012 | 0.0005 | 0.002 |
Bud Periphery | 0.002 | 0 | 0.0003 | 0 | 0.0006 | 0.0002 | 0.0009 | 0.0002 | 0.0008 | 0.0001 | 0.0015 | 0.0001 | 0.0009 | 0.0001 | 0.001 | 0.0001 | 0 | 0.0005 |
Bud Site | 0.0096 | 0.0011 | 0.0007 | 0.0002 | 0.0018 | 0.0004 | 0.0038 | 0.0028 | 0.0025 | 0.0004 | 0.0004 | 0.0002 | 0.0157 | 0.002 | 0.0043 | 0.0003 | 0.0001 | 0.0012 |
Cell Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Cytoplasm | 0.4844 | 0.811 | 0.7724 | 0.8037 | 0.5278 | 0.7463 | 0.4648 | 0.8038 | 0.7371 | 0.6812 | 0.7224 | 0.7255 | 0.6247 | 0.8248 | 0.7844 | 0.6378 | 0.7842 | 0.6256 |
Cytoplasmic Foci | 0.0485 | 0.0076 | 0.0178 | 0.004 | 0.082 | 0.0103 | 0.0539 | 0.0092 | 0.024 | 0.0343 | 0.0065 | 0.0109 | 0.0396 | 0.0131 | 0.0174 | 0.0351 | 0.0142 | 0.0242 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0365 | 0.0023 | 0.0055 | 0.0042 | 0.0023 | 0.0084 | 0.0329 | 0.004 | 0.0073 | 0.0062 | 0.008 | 0.0078 | 0.0249 | 0.0037 | 0.0071 | 0.0046 | 0.0092 | 0.0149 |
Endosome | 0.1624 | 0.0032 | 0.0248 | 0.0098 | 0.0573 | 0.0179 | 0.0818 | 0.0149 | 0.0364 | 0.016 | 0.0145 | 0.0264 | 0.0541 | 0.0028 | 0.01 | 0.0033 | 0.008 | 0.0281 |
Golgi | 0.0185 | 0.0005 | 0.0047 | 0.0003 | 0.0785 | 0.0061 | 0.0149 | 0.0038 | 0.024 | 0.0007 | 0.0023 | 0.0026 | 0.0151 | 0.0005 | 0.0036 | 0.0011 | 0.0061 | 0.0035 |
Lipid Particles | 0.0088 | 0 | 0.0014 | 0.0001 | 0.0047 | 0.0021 | 0.0132 | 0.0002 | 0.0032 | 0.0005 | 0.0001 | 0.0051 | 0.0075 | 0.0001 | 0.0019 | 0.0017 | 0.0093 | 0.0085 |
Mitochondria | 0.0354 | 0.0003 | 0.005 | 0.0005 | 0.0877 | 0.027 | 0.0084 | 0.0018 | 0.0207 | 0.0005 | 0.0793 | 0.0014 | 0.0205 | 0.0003 | 0.0041 | 0.0004 | 0.0022 | 0.0049 |
None | 0.0101 | 0.0022 | 0.0108 | 0.0011 | 0.0005 | 0.001 | 0.031 | 0.0016 | 0.0125 | 0.0036 | 0.0018 | 0.0017 | 0.0081 | 0.0018 | 0.002 | 0.0019 | 0.0009 | 0.0016 |
Nuclear Periphery | 0.0441 | 0.0084 | 0.0109 | 0.0123 | 0.01 | 0.0189 | 0.0648 | 0.0109 | 0.0122 | 0.0136 | 0.015 | 0.0222 | 0.03 | 0.0101 | 0.0164 | 0.011 | 0.02 | 0.0383 |
Nucleolus | 0.0012 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0005 |
Nucleus | 0.0827 | 0.1583 | 0.1377 | 0.158 | 0.0976 | 0.1323 | 0.1662 | 0.1406 | 0.0928 | 0.229 | 0.1392 | 0.1797 | 0.112 | 0.1276 | 0.1087 | 0.2278 | 0.0802 | 0.224 |
Peroxisomes | 0.0052 | 0.0004 | 0.0005 | 0.0001 | 0.0125 | 0.0134 | 0.0046 | 0.0002 | 0.004 | 0.0004 | 0.0002 | 0.0041 | 0.0122 | 0.0059 | 0.0046 | 0.0645 | 0.0595 | 0.0056 |
Punctate Nuclear | 0.0097 | 0.0015 | 0.0017 | 0.0014 | 0.0006 | 0.002 | 0.019 | 0.0017 | 0.0013 | 0.0094 | 0.0013 | 0.0024 | 0.0078 | 0.0026 | 0.0022 | 0.0036 | 0.0024 | 0.0053 |
Vacuole | 0.012 | 0.0009 | 0.0029 | 0.0024 | 0.0018 | 0.0046 | 0.0075 | 0.0017 | 0.0033 | 0.0017 | 0.0029 | 0.005 | 0.003 | 0.0009 | 0.0016 | 0.0011 | 0.0007 | 0.0041 |
Vacuole Periphery | 0.0057 | 0.0002 | 0.0007 | 0.0006 | 0.0011 | 0.0012 | 0.0035 | 0.0004 | 0.0013 | 0.0004 | 0.002 | 0.0015 | 0.0015 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.6377 | 68.4983 | 50.8287 | 66.7758 | 49.674 | 53.608 | 71.8558 | 70.1173 | 48.8009 | 71.0875 |
Translational Efficiency | 1.583 | 1.6718 | 1.9609 | 1.2679 | 1.6531 | 1.4893 | 1.4429 | 1.7266 | 2.1243 | 1.7503 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1413 | 1558 | 459 | 580 | 394 | 1954 | 2987 | 152 | 1807 | 3512 | 3446 | 732 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 936.83 | 1300.61 | 1431.40 | 1331.86 | 1017.30 | 1213.83 | 1449.39 | 1363.56 | 954.38 | 1252.33 | 1446.99 | 1338.44 |
Standard Deviation | 219.55 | 218.79 | 257.95 | 259.44 | 217.31 | 196.65 | 233.64 | 260.32 | 221.57 | 211.21 | 237.10 | 259.94 |
Intensity Change Log 2 | — | 0.473329 | 0.611568 | 0.507583 | — | 0.254821 | 0.510701 | 0.422633 | — | 0.363711 | 0.559938 | 0.463984 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000996 | 0.000462 | 0.001988 | 0.002779 | 0.002810 | 0.000398 | 0.000358 | 0.006372 | 0.001392 | 0.000426 | 0.000575 | 0.003525 |
Bud Neck | 0.005082 | 0.002797 | 0.005589 | 0.011376 | 0.013116 | 0.003483 | 0.002335 | 0.009946 | 0.006834 | 0.003179 | 0.002768 | 0.011079 |
Bud Site | 0.009692 | 0.003022 | 0.012782 | 0.079462 | 0.038491 | 0.004584 | 0.005324 | 0.096069 | 0.015971 | 0.003891 | 0.006317 | 0.082910 |
Cell Periphery | 0.000477 | 0.000101 | 0.000218 | 0.000266 | 0.000988 | 0.000085 | 0.000052 | 0.000778 | 0.000589 | 0.000092 | 0.000074 | 0.000372 |
Cytoplasm | 0.455139* | 0.480680* | 0.595367* | 0.375210* | 0.316991* | 0.578302* | 0.780079* | 0.261895* | 0.425017* | 0.534995* | 0.755476* | 0.351680* |
Cytoplasmic Foci | 0.020821 | 0.010097 | 0.030284 | 0.152557 | 0.024788 | 0.026626 | 0.024226 | 0.204354* | 0.021686 | 0.019293 | 0.025033 | 0.163313 |
Eisosomes | 0.000148 | 0.000008 | 0.000016 | 0.000030 | 0.000040 | 0.000011 | 0.000004 | 0.000031 | 0.000124 | 0.000010 | 0.000006 | 0.000031 |
Endoplasmic Reticulum | 0.007701 | 0.003867 | 0.002909 | 0.002428 | 0.026210 | 0.003402 | 0.000526 | 0.000797 | 0.011737 | 0.003609 | 0.000844 | 0.002089 |
Endosome | 0.001869 | 0.001219 | 0.004495 | 0.042620 | 0.003068 | 0.002036 | 0.002166 | 0.077028 | 0.002131 | 0.001673 | 0.002476 | 0.049765 |
Golgi | 0.000899 | 0.000765 | 0.000709 | 0.012991 | 0.001518 | 0.000917 | 0.000913 | 0.006056 | 0.001034 | 0.000850 | 0.000886 | 0.011551 |
Lipid Particles | 0.002045 | 0.000651 | 0.002063 | 0.007639 | 0.002143 | 0.000858 | 0.001601 | 0.010550 | 0.002066 | 0.000766 | 0.001662 | 0.008243 |
Mitochondria | 0.001375 | 0.001000 | 0.000577 | 0.009824 | 0.011365 | 0.000914 | 0.000820 | 0.006367 | 0.003553 | 0.000952 | 0.000788 | 0.009106 |
Mitotic Spindle | 0.003026 | 0.010705 | 0.034117 | 0.093891 | 0.006434 | 0.007171 | 0.034427 | 0.101782* | 0.003769 | 0.008738 | 0.034386 | 0.095529 |
None | 0.036858 | 0.006812 | 0.005200 | 0.000891 | 0.007672 | 0.006123 | 0.004359 | 0.000758 | 0.030495 | 0.006429 | 0.004471 | 0.000863 |
Nuclear Periphery | 0.001135 | 0.001416 | 0.001695 | 0.000521 | 0.001167 | 0.001024 | 0.000589 | 0.002431 | 0.001142 | 0.001198 | 0.000736 | 0.000918 |
Nuclear Periphery Foci | 0.007875 | 0.003999 | 0.006357 | 0.008394 | 0.005126 | 0.003706 | 0.002692 | 0.005478 | 0.007276 | 0.003836 | 0.003180 | 0.007788 |
Nucleolus | 0.002325 | 0.000608 | 0.000387 | 0.001171 | 0.002322 | 0.000529 | 0.000324 | 0.000496 | 0.002324 | 0.000564 | 0.000333 | 0.001031 |
Nucleus | 0.421283* | 0.454942* | 0.237236* | 0.017491 | 0.503512* | 0.338135* | 0.113057 | 0.009216 | 0.439212* | 0.389953* | 0.129598 | 0.015772 |
Peroxisomes | 0.000986 | 0.000187 | 0.000729 | 0.017931 | 0.001398 | 0.000854 | 0.000728 | 0.026795 | 0.001076 | 0.000558 | 0.000728 | 0.019772 |
Vacuole | 0.018532 | 0.015862 | 0.055753 | 0.150987 | 0.017666 | 0.020371 | 0.024756 | 0.159301 | 0.018343 | 0.018371 | 0.028885 | 0.152714 |
Vacuole Periphery | 0.001735 | 0.000799 | 0.001527 | 0.011541 | 0.013175 | 0.000472 | 0.000662 | 0.013498 | 0.004229 | 0.000617 | 0.000777 | 0.011948 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.32 | -0.97 | -2.71 | -3.95 | -0.81 | 7.72 | 7.67 | -0.78 | -2.13 | -2.13 | 5.66 | 4.45 | -2.42 | -4.39 | -4.11 |
Bud Neck | 2.49 | -2.63 | -2.98 | -4.65 | -1.25 | 6.37 | 6.82 | 2.52 | -4.23 | -4.78 | 4.97 | 4.55 | -2.00 | -5.31 | -5.02 |
Bud Site | 5.56 | -1.47 | -10.15 | -11.61 | -9.23 | 8.07 | 7.79 | -3.03 | -6.37 | -6.24 | 9.11 | 6.89 | -10.21 | -13.15 | -12.52 |
Cell Periphery | 4.92 | 2.36 | 3.40 | -4.32 | 2.39 | 12.33 | 12.69 | 1.77 | -1.40 | -1.49 | 8.17 | 8.24 | 3.73 | -2.81 | -2.88 |
Cytoplasm | -1.56 | -4.23 | 4.02 | 5.22 | 6.81 | -17.30 | -29.81 | 1.57 | 12.15 | 19.20 | -12.45 | -35.24 | 4.16 | 12.60 | 27.16 |
Cytoplasmic Foci | 6.26 | -1.98 | -15.81 | -17.67 | -14.31 | -0.90 | -1.00 | -10.22 | -10.16 | -10.16 | 1.59 | -2.77 | -18.69 | -19.34 | -18.02 |
Eisosomes | 2.63 | 2.39 | 2.32 | -6.19 | -1.13 | 7.17 | 9.02 | 2.43 | -3.69 | -5.46 | 2.76 | 2.85 | 2.40 | -6.75 | -8.50 |
Endoplasmic Reticulum | 6.71 | 6.89 | 10.83 | 6.23 | 3.04 | 9.88 | 11.27 | 11.26 | 10.98 | -0.03 | 11.97 | 16.92 | 15.05 | 7.77 | -1.70 |
Endosome | 2.64 | -4.80 | -11.88 | -12.12 | -10.69 | 2.00 | 1.71 | -4.86 | -4.96 | -4.94 | 1.91 | -1.67 | -11.78 | -11.92 | -11.63 |
Golgi | 0.61 | 1.65 | -4.64 | -4.70 | -4.80 | 1.50 | 2.03 | -2.15 | -2.84 | -3.03 | 1.04 | 1.78 | -4.93 | -5.07 | -5.16 |
Lipid Particles | 3.66 | -1.80 | -6.50 | -8.50 | -5.78 | 2.94 | -0.64 | -3.59 | -4.28 | -3.50 | 4.23 | -1.32 | -7.43 | -9.41 | -7.14 |
Mitochondria | 1.24 | 3.39 | -2.23 | -2.41 | -2.55 | 3.93 | 4.02 | 2.47 | -1.29 | -1.42 | 4.17 | 4.64 | -1.02 | -2.67 | -2.83 |
Mitotic Spindle | -3.88 | -5.98 | -10.30 | -9.04 | -5.47 | -0.46 | -10.10 | -5.31 | -5.28 | -3.84 | -4.05 | -14.09 | -11.46 | -10.69 | -6.85 |
None | 7.92 | 8.54 | 9.54 | 10.44 | 10.56 | 1.29 | 2.72 | 5.32 | 10.13 | 11.42 | 8.07 | 8.80 | 9.97 | 15.02 | 19.90 |
Nuclear Periphery | -1.59 | -3.34 | 2.67 | 8.32 | 5.69 | 0.39 | 1.50 | -0.29 | -0.37 | -0.58 | -0.63 | 0.52 | 1.37 | 2.07 | 1.27 |
Nuclear Periphery Foci | 4.22 | 1.05 | 0.30 | -2.73 | -0.47 | 0.90 | 1.26 | -0.36 | -1.21 | -1.50 | 4.67 | 4.69 | 0.17 | -3.12 | -3.14 |
Nucleolus | 5.21 | 7.35 | 4.34 | -0.70 | -1.80 | 1.94 | 2.08 | 2.08 | 0.91 | 0.09 | 5.88 | 6.84 | 4.68 | -0.89 | -1.96 |
Nucleus | -3.62 | 11.06 | 47.16* | 59.42* | 23.14 | 9.91 | 24.02 | 31.08 | 48.37* | 27.27 | 5.34 | 37.68 | 55.84 | 74.48* | 37.95 |
Peroxisomes | 1.86 | 0.65 | -6.71 | -7.32 | -7.02 | 0.94 | 1.25 | -4.95 | -5.14 | -5.19 | 1.34 | 0.90 | -8.19 | -8.67 | -8.57 |
Vacuole | -0.71 | -10.79 | -18.51 | -18.31 | -8.44 | -3.76 | -12.68 | -10.36 | -9.95 | -8.92 | -2.36 | -14.90 | -21.21 | -20.79 | -17.26 |
Vacuole Periphery | 1.57 | 0.91 | -3.65 | -4.54 | -4.19 | 3.91 | 3.89 | 1.16 | -1.84 | -1.82 | 4.26 | 4.26 | -1.93 | -4.89 | -4.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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