ORF
Human Ortholog
Description Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.98 0.97 0.93 0.86 0.77 0.74 0.77 0.74 0.72 – 1.0 0.98 1.0 0.96 0.97 0.96 0.97 0.96 0.77 0.85 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.05 0.13 0.1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0.07 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.13 0.2 0.16 0.13 0.14 0.16 – 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 1 0 3 2 – 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 2 1 2 3 3 6 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 5 7 8 13 15 13 7 11 11 16 – 0 0 2 2 0 0 0 0 0 0 0
Cytoplasm 118 57 257 281 372 312 427 484 275 337 275 360 – 17 63 47 104 78 118 86 247 22 56 73
Endoplasmic Reticulum 1 1 3 2 2 7 7 10 7 13 4 13 – 0 1 2 1 4 0 0 3 0 0 0
Endosome 0 0 0 0 0 0 0 0 1 1 0 0 – 0 0 0 0 0 1 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 2 6 7 46 23 49 49 – 0 0 1 2 0 0 0 0 0 0 0
Nucleus 1 0 1 2 4 6 8 24 10 10 9 14 – 0 0 3 4 2 0 0 1 1 2 1
Nuclear Periphery 0 0 0 1 0 0 0 4 0 0 1 2 – 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 2 9 8 65 125 59 59 53 82 – 0 2 0 1 1 1 0 1 0 1 0
Unique Cell Count 119 58 261 286 385 337 497 632 372 438 372 499 17 64 47 108 80 124 89 259 29 66 85
Labelled Cell Count 120 59 267 295 395 349 531 669 409 458 408 544 17 66 55 115 85 124 89 259 29 66 85


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.4 12.7 12.5 12.0 12.0 10.7 9.9 9.8 8.7 9.0 8.6 8.4 – 13.8 14.1 10.9 13.0 13.4 – 13.6 15.9
Std Deviation (1e-4) 2.7 1.9 2.0 2.0 1.5 1.8 1.7 1.7 1.9 1.6 1.6 1.6 – 1.5 10.8 1.9 2.8 3.1 – 3.6 3.8
Intensity Change (Log2) – – – -0.06 -0.06 -0.22 -0.35 -0.35 -0.53 -0.47 -0.54 -0.57 – 0.14 0.17 -0.21 0.06 0.09 – 0.12 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0.4 0.1 1.4 0.9 0.1 0 0.5 0.8 1.0 – 0 0 0 0 0
Cytoplasm -0.2 -1.4 -3.3 -5.5 -7.9 -8.3 -7.7 -8.3 -8.8 – 0.5 0 0.9 -1.3 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 1.6 0 1.3 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.9 3.8 6.1 5.2 – 0 0 0 0 0
Nucleus 0 0 0 0 2.8 0 0 0 2.3 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 5.9 7.6 6.5 6.0 6.1 6.7 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.3689 7.9322 7.1368 5.9076 6.4976 6.7314 7.4758 9.4438 8.4567 8.2492 8.0107 8.5396 5.6697 8.4507 6.7873 6.467 6.4332 7.2631
Actin 0.0195 0.0003 0.008 0.0002 0.0083 0.0056 0.0544 0.0006 0.0085 0.0011 0.022 0.0008 0.0016 0.0002 0.0007 0.0002 0.007 0.0059
Bud 0.0003 0.0001 0.0002 0.0001 0.0015 0.0006 0.0007 0 0.0001 0.0002 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0007 0.0001 0.0006 0.0002 0.0011 0.0009 0.0034 0.0002 0.0003 0.0005 0.0355 0.0009 0.0003 0.0001 0.0003 0.0003 0.0004 0.0015
Bud Periphery 0.0002 0 0.0001 0 0.0021 0.0005 0.001 0 0.0001 0.0001 0.0006 0 0.0001 0 0.0001 0 0.0005 0
Bud Site 0.0003 0.0002 0.0024 0.0001 0.0059 0.0002 0.0031 0.0003 0.0002 0.0002 0.0012 0 0.0005 0.0001 0.0004 0 0.0002 0.0001
Cell Periphery 0.0001 0 0.0001 0 0.0001 0 0.0005 0.0001 0.0001 0 0.0002 0 0 0 0 0 0 0
Cytoplasm 0.8864 0.9837 0.9485 0.9888 0.9423 0.9614 0.8348 0.9841 0.9717 0.9769 0.8548 0.9837 0.8881 0.9932 0.9588 0.9923 0.853 0.958
Cytoplasmic Foci 0.0069 0.0013 0.0033 0.0018 0.0026 0.0067 0.0202 0.0011 0.0016 0.0058 0.0178 0.0026 0.0345 0.0007 0.0037 0.0012 0.0032 0.0085
Eisosomes 0.0004 0 0.0002 0 0.0001 0 0.0009 0 0 0 0.0001 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0046 0.0016 0.0021 0.0018 0.0028 0.0018 0.0063 0.0011 0.003 0.0019 0.0014 0.0018 0.0093 0.0011 0.0005 0.0006 0.0003 0.0014
Endosome 0.0199 0.0011 0.0035 0.0011 0.003 0.0063 0.0139 0.0003 0.002 0.0017 0.0073 0.0007 0.0077 0.0003 0.0053 0.0003 0.0057 0.0018
Golgi 0.0039 0.0001 0.0006 0.0001 0.0009 0.0013 0.0059 0.0001 0.0013 0.0002 0.0073 0.0001 0.0022 0 0.0028 0 0.0206 0.0033
Lipid Particles 0.0092 0 0.0007 0 0.0007 0.0003 0.0065 0.0001 0.0002 0 0.0038 0 0.0144 0 0.0026 0 0.0013 0.0004
Mitochondria 0.0039 0.0001 0.0084 0.0001 0.001 0.0013 0.0083 0 0.0003 0.0001 0.0357 0.0001 0.0008 0 0.0183 0 0.1 0.0007
None 0.0237 0.0021 0.0033 0.0015 0.0024 0.0021 0.0133 0.0106 0.007 0.0081 0.0053 0.005 0.0219 0.0025 0.0014 0.0015 0.0008 0.0011
Nuclear Periphery 0.0065 0.0011 0.0046 0.0007 0.0061 0.0018 0.0054 0.0003 0.0011 0.0004 0.0003 0.0006 0.0018 0.0003 0.0004 0.0005 0.0002 0.0004
Nucleolus 0.0003 0 0 0 0.0013 0 0.0009 0 0 0 0.0001 0 0.0002 0 0.0001 0 0 0
Nucleus 0.0062 0.0075 0.0057 0.0028 0.011 0.0064 0.0044 0.001 0.0018 0.0019 0.0013 0.0031 0.0019 0.0011 0.0021 0.0025 0.0018 0.0023
Peroxisomes 0.0019 0 0.0065 0 0.0005 0.001 0.0089 0 0.0001 0.0001 0.0032 0 0.0104 0 0.0014 0 0.0036 0.0139
Punctate Nuclear 0.0019 0.0002 0.0003 0.0001 0.0047 0.0008 0.0056 0.0001 0.0003 0.0003 0.0007 0.0003 0.0031 0.0001 0.0003 0.0002 0.0001 0.0002
Vacuole 0.0021 0.0006 0.0006 0.0005 0.0012 0.0008 0.0012 0.0001 0.0003 0.0003 0.0006 0.0002 0.0006 0.0001 0.0004 0.0001 0.0004 0.0002
Vacuole Periphery 0.0011 0.0001 0.0003 0.0001 0.0003 0.0003 0.0005 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0003 0 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.342 28.0365 61.4673 58.2107 52.6776 39.8943 44.1322 47.832 53.1135 50.477
Translational Efficiency 2.6662 4.2244 2.1782 2.1931 1.8373 2.5667 2.4303 2.1177 2.0692 2.0925

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1691 192 1572 77 1508 1679 1319 1313 3199 1871 2891 1390

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1012.11 1342.78 1397.88 1932.26 992.33 1316.83 1445.15 1693.80 1002.79 1319.49 1419.45 1707.01
Standard Deviation 179.91 248.75 229.15 374.59 351.47 181.39 231.90 303.24 274.66 189.57 231.61 312.42
Intensity Change Log 2 — 0.407857 0.465874 0.932923 — 0.408177 0.542327 0.771372 — 0.408015 0.504230 0.855204

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000132 0.001642 0.000180 0.011133 0.000095 0.000257 0.000340 0.000323 0.000114 0.000399 0.000253 0.000921
Bud Neck 0.000768 0.001168 0.001013 0.004428 0.000361 0.000904 0.001676 0.000719 0.000576 0.000931 0.001315 0.000924
Bud Site 0.005161 0.013212 0.003994 0.040118 0.002509 0.007121 0.004757 0.005747 0.003911 0.007746 0.004342 0.007651
Cell Periphery 0.000075 0.000143 0.000079 0.000367 0.000125 0.000042 0.000117 0.000113 0.000099 0.000052 0.000096 0.000127
Cytoplasm 0.843211* 0.851570* 0.784738* 0.666043* 0.888783* 0.879380* 0.700855* 0.871953* 0.864693* 0.876526* 0.746467* 0.860546*
Cytoplasmic Foci 0.075118 0.063573 0.012166 0.028596 0.026304 0.042627 0.012903 0.009499 0.052107 0.044776 0.012502 0.010556
Eisosomes 0.000008 0.000009 0.000006 0.000014 0.000004 0.000006 0.000013 0.000002 0.000006 0.000006 0.000009 0.000003
Endoplasmic Reticulum 0.001095 0.000367 0.000641 0.000161 0.000452 0.000177 0.002290 0.000086 0.000792 0.000197 0.001393 0.000091
Endosome 0.002409 0.001094 0.001797 0.007979 0.000870 0.001549 0.002967 0.001230 0.001683 0.001502 0.002331 0.001604
Golgi 0.001302 0.001348 0.000525 0.083183 0.000483 0.002204 0.000303 0.000631 0.000916 0.002116 0.000423 0.005204
Lipid Particles 0.000651 0.000854 0.000725 0.002886 0.000222 0.000228 0.001306 0.000470 0.000449 0.000292 0.000990 0.000604
Mitochondria 0.000779 0.000325 0.000113 0.005710 0.000063 0.001228 0.000285 0.000082 0.000441 0.001135 0.000191 0.000394
Mitotic Spindle 0.000546 0.001977 0.008556 0.001240 0.000519 0.002700 0.006785 0.003278 0.000533 0.002626 0.007748 0.003165
None 0.005105 0.003508 0.004888 0.000815 0.007465 0.004520 0.005842 0.006427 0.006218 0.004416 0.005323 0.006116
Nuclear Periphery 0.000060 0.000049 0.000339 0.000095 0.000044 0.000063 0.000368 0.000076 0.000052 0.000062 0.000353 0.000077
Nuclear Periphery Foci 0.001738 0.005622 0.003401 0.002289 0.002588 0.001442 0.003634 0.002341 0.002139 0.001871 0.003507 0.002339
Nucleolus 0.000177 0.000059 0.000148 0.000064 0.000135 0.000156 0.000199 0.000070 0.000157 0.000146 0.000171 0.000070
Nucleus 0.029996 0.029938 0.106694 0.041842 0.049024 0.028895 0.133762* 0.080309 0.038966 0.029002 0.119044 0.078178
Peroxisomes 0.000300 0.000659 0.000161 0.037227 0.000101 0.000631 0.000256 0.000211 0.000206 0.000634 0.000204 0.002261
Vacuole 0.030538 0.022775 0.069681 0.060054 0.019444 0.024836 0.121014 0.016224 0.025309 0.024624 0.093102 0.018652
Vacuole Periphery 0.000829 0.000109 0.000157 0.005755 0.000409 0.001035 0.000328 0.000208 0.000631 0.000940 0.000235 0.000515

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.59 -6.73 -1.54 -0.37 -1.46 -2.11 -6.76 -4.12 -0.96 -0.08 -2.44 -9.10 -3.70 -0.45 -1.73
Bud Neck -1.94 -5.88 -2.14 -1.95 -1.74 -3.25 -14.93 -11.13 -2.78 6.73 -2.42 -13.72 -6.68 -3.38 2.24
Bud Site -3.98 0.32 -4.04 -2.14 -4.08 -5.10 -8.42 -6.12 0.15 0.01 -4.54 -4.93 -5.34 -0.16 -2.60
Cell Periphery -3.43 -2.82 -4.95 -4.25 -4.69 2.12 -0.08 -0.82 -13.89 -2.81 2.43 -0.85 -3.89 -12.44 -6.04
Cytoplasm 0.48 20.43 8.92 8.31 4.67 2.85 34.45 12.80 9.26 -23.74 -1.06 38.02 11.12 10.84 -20.94
Cytoplasmic Foci 1.47 21.11 6.11 2.97 -4.29 -7.25 7.63 6.44 13.32 -1.22 2.04 21.60 18.71 14.01 -2.49
Eisosomes -0.50 -1.18 -2.74 -1.76 -2.45 -4.29 -14.91 0.45 4.84 15.52 -0.28 -10.53 4.27 3.91 15.65
Endoplasmic Reticulum 2.83 2.26 4.41 1.95 8.46 4.05 -2.46 5.54 4.32 3.69 4.87 -0.49 5.84 4.70 5.50
Endosome 4.19 3.35 0.92 -0.45 0.03 -3.33 -3.86 0.43 2.16 3.10 1.17 -0.81 2.78 2.04 3.05
Golgi 0.20 5.57 -0.94 -0.95 -1.03 -2.73 2.33 0.43 2.82 -1.20 -2.00 5.90 -0.12 1.23 -1.19
Lipid Particles -1.54 -4.12 -5.35 -5.09 -5.00 -0.57 -6.58 -9.79 -9.65 1.34 2.49 -7.85 -8.30 -9.86 -1.72
Mitochondria 0.94 1.33 0.02 -0.64 -0.86 -2.12 -1.85 -0.70 2.11 1.74 -1.14 1.10 1.31 2.06 1.08
Mitotic Spindle -1.86 -6.87 -2.12 -1.43 2.06 -2.92 -4.38 -3.88 -1.52 0.58 -3.11 -8.13 -4.10 -1.71 2.41
None 1.20 0.86 3.83 1.69 9.32 3.32 3.27 2.83 -0.79 -0.79 2.64 2.74 2.16 -0.66 -0.62
Nuclear Periphery -0.45 -21.38 -6.23 -5.91 7.59 -1.13 -24.54 -12.34 -4.72 17.08 -0.76 -31.43 -11.88 -5.51 21.93
Nuclear Periphery Foci -4.76 -6.28 -4.81 1.28 -1.41 4.01 -2.80 -1.93 -6.96 1.42 1.07 -6.11 -4.80 -5.38 1.44
Nucleolus 2.57 -0.52 2.35 -0.46 3.26 -0.81 -4.46 3.08 2.04 9.08 0.06 -1.63 2.63 1.97 5.10
Nucleus -1.32 -29.58 -0.70 0.06 11.14 9.53 -22.98 -12.73 -22.64 11.56 5.83 -36.90 -17.70 -21.82 13.65
Peroxisomes -1.14 3.73 -0.98 -0.91 -1.01 -4.69 -2.61 -0.91 2.64 -0.18 -4.03 1.53 -0.93 0.61 -1.08
Vacuole -0.02 -19.90 -8.79 -8.71 -5.58 -4.12 -26.45 -10.76 -7.77 20.19 -1.94 -32.64 -11.50 -10.16 17.40
Vacuole Periphery 4.77 4.55 0.04 -0.98 -0.93 -1.17 0.97 1.36 1.94 1.25 -0.58 3.67 3.44 1.82 0.12
ORF
Human Ortholog
Description Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YBL036C

YBL036C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybl036c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available