ORF
Human Ortholog
Description 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.97 0.95 0.9 0.76 0.78 0.66 0.65 0.6 0.65 0.99 0.99 0.99 0.99 0.97 0.99 0.94 0.96 0.95 0.94 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.18 0.32 0.24 0.42 0.4 0.5 0.44 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.08 0.1 0.1 0.15 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 1 2 1 0 4 7 3 12 8 9 7 1 0 0 0 0 0 3 0 0 0 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 7 4 3 7 8 7 12 8 9 3 3 0 4 11 2 5 0 0 0 0 0 0
Cytoplasm 246 124 304 304 428 397 587 481 297 357 262 280 394 416 448 142 166 153 238 122 286 148 473 386
Endoplasmic Reticulum 0 0 2 2 0 1 5 6 2 4 0 1 1 3 2 6 7 8 0 0 2 0 1 1
Endosome 0 0 0 1 8 2 5 9 7 6 4 0 0 0 1 1 0 0 2 1 0 0 3 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0 0 2
Mitochondria 4 1 2 12 25 78 250 145 188 217 217 188 0 0 0 0 2 1 1 0 4 0 1 2
Nucleus 1 1 0 0 1 2 4 7 3 7 5 4 0 1 2 2 2 1 0 0 0 0 1 4
Nuclear Periphery 0 0 0 0 0 1 5 2 2 1 1 2 0 0 1 0 0 0 1 0 0 0 2 0
Nucleolus 0 0 0 1 0 0 1 2 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 1 0 0 0 1 1
Vac/Vac Membrane 1 0 2 3 14 13 43 52 44 56 66 34 0 0 0 2 3 4 2 1 2 1 4 2
Unique Cell Count 250 126 308 314 449 440 774 614 451 549 436 431 397 419 451 144 171 155 254 128 303 158 504 416
Labelled Cell Count 253 128 319 328 479 505 916 714 570 666 577 519 399 421 458 166 182 172 254 128 303 158 504 416


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.2 6.5 6.5 6.4 5.6 5.2 5.3 5.1 5.1 4.9 5.0 8.3 8.1 7.5 9.6 9.9 8.7 7.9 8.7 8.9
Std Deviation (1e-4) 0.9 1.1 1.5 1.5 1.7 1.4 1.3 1.4 1.3 1.2 1.5 1.0 1.0 1.2 1.1 1.5 1.7 1.8 1.3 1.7 1.5
Intensity Change (Log2) – – – -0.01 -0.03 -0.23 -0.32 -0.29 -0.37 -0.36 -0.41 -0.39 0.34 0.31 0.19 0.55 0.6 0.41 0.28 0.41 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2.0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 0 -0.7 0 0 0.3 -0.9 -0.2 0 0 0 0 2.7 0 0
Cytoplasm -1.6 -2.6 -4.7 -8.9 -8.2 -10.9 -11.3 -12.2 -11.1 0.7 0.8 0.9 -0.1 -1.3 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 3.6 7.4 11.1 9.0 12.8 12.5 14.5 13.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.3 2.2 3.6 4.8 5.2 5.3 6.8 4.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4152 5.6829 5.1865 4.6145 4.74 5.2321 0.6092 2.0365 1.316 1.6821 0.7792 1.4087 1.2452 2.2396 1.3812 1.5315 1.1022 1.3309
Actin 0.0003 0.0097 0.0012 0.0015 0.0004 0.0015 0.032 0.002 0.0174 0.0164 0.0197 0.0049 0.0459 0.0005 0.0117 0.014 0.003 0.0005
Bud 0 0.0031 0.0002 0.0015 0.0001 0.0001 0.0006 0.0007 0.0008 0.0003 0.002 0.0008 0.0005 0.017 0.007 0.0061 0.0002 0.0003
Bud Neck 0.0001 0.0008 0.0005 0.0006 0.0002 0.0008 0.0015 0.0014 0.0008 0.0012 0.0078 0.0013 0.0015 0.0002 0.0003 0.0202 0.0003 0.0032
Bud Periphery 0 0.0025 0.0002 0.0024 0.0001 0.0001 0.0009 0.0009 0.0007 0.0003 0.0013 0.0008 0.0005 0.0033 0.0015 0.0027 0.0001 0.0003
Bud Site 0.0002 0.0288 0.0006 0.0006 0.0001 0.0001 0.0023 0.0081 0.0092 0.0019 0.0021 0.0003 0.0033 0.0071 0.0019 0.0041 0.0006 0.0001
Cell Periphery 0.0005 0.001 0.0003 0.0002 0.0001 0.0001 0.0004 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0003 0.0002 0.0006 0.0001 0.0001
Cytoplasm 0.7859 0.7802 0.7467 0.626 0.7816 0.7904 0.6326 0.8807 0.8587 0.9372 0.6249 0.9061 0.6781 0.8898 0.86 0.8009 0.8202 0.9326
Cytoplasmic Foci 0.0039 0.0054 0.0073 0.0262 0.0056 0.0071 0.0457 0.0194 0.011 0.0071 0.0106 0.0139 0.041 0.0147 0.0106 0.0141 0.0102 0.0141
Eisosomes 0.0001 0.0001 0.0001 0.0001 0 0 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.0081 0.0031 0.0048 0.004 0.0037 0.0041 0.0252 0.0033 0.0025 0.0021 0.006 0.0027 0.0052 0.0035 0.0035 0.0018 0.0129 0.0026
Endosome 0.0019 0.0015 0.0034 0.0136 0.0058 0.0032 0.0833 0.0161 0.0182 0.0065 0.06 0.0095 0.0236 0.0021 0.0069 0.0038 0.055 0.0122
Golgi 0.0002 0.0004 0.0006 0.0011 0.0003 0.0003 0.0137 0.0062 0.007 0.006 0.0278 0.0052 0.0066 0.0002 0.0016 0.0025 0.0023 0.0007
Lipid Particles 0.0004 0.0002 0.0003 0.0012 0.0003 0.0002 0.0189 0.0054 0.0019 0.0023 0.0167 0.0049 0.0149 0.0002 0.0026 0.0025 0.0023 0.0002
Mitochondria 0.0001 0.0002 0.0003 0.0016 0.0002 0.0002 0.0055 0.004 0.0125 0.003 0.0197 0.0206 0.0026 0.0009 0.0094 0.0243 0.002 0.0009
None 0.1949 0.156 0.2281 0.3051 0.1989 0.1882 0.0723 0.0348 0.044 0.0102 0.0244 0.0189 0.1359 0.0545 0.0686 0.0786 0.0229 0.0188
Nuclear Periphery 0.0006 0.0007 0.001 0.0019 0.0006 0.0005 0.0295 0.0009 0.0012 0.0007 0.0692 0.0007 0.0085 0.0007 0.0028 0.0019 0.0452 0.0044
Nucleolus 0 0.0001 0.0001 0.0004 0 0.0001 0.0005 0.0021 0.0002 0.0001 0.0039 0.0001 0.0019 0.0005 0.0016 0.0019 0.0006 0.001
Nucleus 0.0008 0.0014 0.0013 0.0024 0.0006 0.0009 0.0049 0.0014 0.0015 0.001 0.0519 0.0016 0.0051 0.0016 0.0033 0.006 0.01 0.0025
Peroxisomes 0 0.0002 0.0001 0.0013 0.0001 0.0001 0.0106 0.008 0.0076 0.0021 0.002 0.0058 0.0121 0.0003 0.0009 0.0066 0.0003 0.0001
Punctate Nuclear 0.0001 0.0006 0.001 0.0057 0.0002 0.0009 0.0045 0.0016 0.0013 0.0004 0.0365 0.0004 0.0067 0.0012 0.0033 0.0024 0.0043 0.0007
Vacuole 0.0016 0.0035 0.0017 0.0023 0.0012 0.0008 0.0095 0.0023 0.0025 0.001 0.0067 0.001 0.0047 0.0011 0.0016 0.0034 0.005 0.0033
Vacuole Periphery 0.0001 0.0003 0.0002 0.0006 0.0001 0.0001 0.005 0.0004 0.0009 0.0003 0.0063 0.0004 0.0011 0.0002 0.0005 0.0014 0.0025 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.5063 24.3259 18.0509 15.8515 15.3646 24.565 15.0749 15.2618 12.7185 21.0942
Translational Efficiency 0.9281 0.5079 0.6979 0.6642 0.877 0.543 1.0318 0.8517 0.9648 0.7913

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
629 1081 1922 964 1276 1625 221 708 1905 2706 2143 1672

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 763.49 904.93 922.00 947.99 758.54 851.69 1058.68 945.86 760.17 872.96 936.10 947.09
Standard Deviation 102.13 141.14 125.83 138.85 113.13 105.50 125.28 133.20 109.64 123.78 132.46 136.49
Intensity Change Log 2 — 0.245197 0.272157 0.312263 — 0.167103 0.480969 0.318401 — 0.206805 0.379999 0.315325

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000932 0.000728 0.000207 0.001549 0.000305 0.000654 0.000691 0.001342 0.000512 0.000683 0.000257 0.001461
Bud Neck 0.013568 0.013428 0.002668 0.004619 0.013728 0.016832 0.002284 0.006202 0.013675 0.015472 0.002629 0.005289
Bud Site 0.023198 0.043909 0.009932 0.033828 0.017043 0.029516 0.007648 0.042852 0.019076 0.035265 0.009697 0.037649
Cell Periphery 0.000157 0.000088 0.000075 0.000229 0.000150 0.000089 0.000257 0.000209 0.000152 0.000089 0.000094 0.000220
Cytoplasm 0.447249* 0.529631* 0.705654* 0.521439* 0.524298* 0.525540* 0.431804* 0.540861* 0.498858* 0.527174* 0.677413* 0.529663*
Cytoplasmic Foci 0.305386* 0.206604* 0.025924 0.037746 0.224017* 0.193592* 0.032492 0.042635 0.250884* 0.198790* 0.026601 0.039816
Eisosomes 0.000117 0.000045 0.000017 0.000061 0.000100 0.000064 0.000098 0.000055 0.000106 0.000057 0.000025 0.000058
Endoplasmic Reticulum 0.001684 0.000971 0.001372 0.004004 0.002506 0.000670 0.006037 0.003028 0.002234 0.000790 0.001853 0.003591
Endosome 0.039072 0.035868 0.002188 0.007393 0.029936 0.032460 0.009648 0.011605 0.032953 0.033821 0.002958 0.009177
Golgi 0.007220 0.006571 0.000643 0.005064 0.004762 0.007061 0.000217 0.003769 0.005574 0.006865 0.000599 0.004515
Lipid Particles 0.005340 0.001591 0.000748 0.002644 0.005853 0.001993 0.003438 0.001381 0.005683 0.001832 0.001025 0.002109
Mitochondria 0.003549 0.003382 0.000366 0.001284 0.003334 0.003468 0.000271 0.004510 0.003405 0.003434 0.000357 0.002650
Mitotic Spindle 0.001644 0.006730 0.007849 0.013560 0.001984 0.010204 0.001200 0.021830 0.001872 0.008816 0.007163 0.017062
None 0.000522 0.000464 0.002420 0.002286 0.001465 0.000394 0.004357 0.001672 0.001154 0.000422 0.002619 0.002026
Nuclear Periphery 0.000277 0.000123 0.000601 0.001180 0.000314 0.000173 0.001464 0.000524 0.000302 0.000153 0.000690 0.000902
Nuclear Periphery Foci 0.000901 0.001085 0.001742 0.004715 0.001004 0.000445 0.007761 0.002942 0.000970 0.000701 0.002363 0.003964
Nucleolus 0.000346 0.000420 0.000149 0.000190 0.001125 0.000429 0.000420 0.000203 0.000868 0.000425 0.000177 0.000196
Nucleus 0.030672 0.020267 0.147331* 0.118530 0.053038 0.024426 0.193219* 0.080825 0.045653 0.022765 0.152063* 0.102564
Peroxisomes 0.007746 0.010707 0.000515 0.002633 0.003513 0.007447 0.004591 0.003312 0.004911 0.008750 0.000935 0.002921
Vacuole 0.107425 0.114297 0.089405 0.235660* 0.109106 0.141835 0.291833* 0.226600* 0.108551 0.130834 0.110280 0.231824*
Vacuole Periphery 0.002994 0.003092 0.000195 0.001387 0.002420 0.002708 0.000269 0.003644 0.002609 0.002861 0.000203 0.002342

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.44 1.46 0.03 -0.99 -3.74 -2.32 -3.13 -2.79 -1.01 -1.23 -0.93 1.38 -1.88 -1.42 -4.66
Bud Neck 0.18 6.76 6.02 8.79 -2.24 -1.76 9.76 7.92 9.97 -1.63 -1.27 11.65 10.22 13.30 -2.45
Bud Site -5.85 6.15 1.48 6.94 -3.78 -4.91 4.90 -1.03 2.54 -4.34 -7.75 6.54 -0.08 6.46 -5.30
Cell Periphery 3.32 3.95 -0.23 -5.27 -6.40 5.88 -3.93 0.58 -2.98 3.60 6.55 6.25 -0.03 -5.94 -5.66
Cytoplasm -5.74 -15.74 1.37 8.02 20.53 -0.12 9.89 5.13 5.33 -5.79 -3.32 -13.25 6.25 9.79 19.39
Cytoplasmic Foci 9.56 32.02 31.69 30.10 -0.42 4.52 32.42 32.62 31.12 -3.15 8.98 46.37 45.18 43.74 -2.62
Eisosomes 7.95 11.17 6.77 -2.78 -12.53 5.92 0.61 8.30 2.43 6.52 9.82 17.09 10.66 0.60 -11.31
Endoplasmic Reticulum 2.24 0.22 -4.18 -6.70 -5.59 3.78 -5.54 0.89 -3.68 6.98 4.22 0.28 -1.25 -8.03 -2.44
Endosome 0.97 11.15 10.50 13.50 -3.74 -0.76 11.28 12.09 14.77 2.09 -0.09 18.11 16.56 20.67 -3.29
Golgi 0.69 6.28 4.10 4.44 -1.21 -2.16 6.73 3.59 5.58 -1.21 -1.48 8.14 5.02 6.80 -1.45
Lipid Particles 4.59 5.68 3.91 -1.77 -5.57 5.95 4.43 7.01 1.04 5.53 7.57 9.56 7.64 -0.53 -5.37
Mitochondria 0.20 3.15 2.81 3.11 -2.03 -0.07 3.72 1.69 1.97 -1.29 0.06 4.73 3.30 3.88 -1.66
Mitotic Spindle -2.76 -3.74 -4.15 -1.57 -1.09 -4.34 -0.07 -3.29 -0.27 -3.30 -5.15 -4.24 -5.33 -0.90 -1.77
None 0.68 -12.30 -7.59 -8.40 -0.59 3.58 -1.93 -1.15 -7.21 1.32 3.62 -4.97 -4.01 -11.02 0.58
Nuclear Periphery 2.45 -8.62 -8.34 -10.32 -4.35 2.52 -12.61 -8.81 -12.38 8.99 3.59 -14.18 -10.59 -13.02 -2.23
Nuclear Periphery Foci -1.22 -6.36 -9.25 -8.12 -6.20 5.76 -10.29 -7.93 -10.93 7.24 2.76 -10.57 -11.81 -13.13 -4.01
Nucleolus -1.19 4.94 3.78 4.06 -2.11 2.75 3.33 3.87 4.94 2.24 2.58 4.13 4.08 6.21 -0.55
Nucleus 3.71 -30.97 -20.18 -23.26 4.94 10.66 -11.50 -8.73 -16.22 8.08 11.29 -30.80 -19.61 -27.93 10.52
Peroxisomes -1.86 7.17 6.16 9.02 -1.51 -5.62 1.27 5.18 8.60 0.32 -5.47 10.44 8.09 12.42 -1.78
Vacuole -1.44 -3.88 -22.98 -23.23 -21.77 -6.51 -14.66 -22.19 -18.55 1.22 -5.98 -9.45 -32.48 -28.97 -23.74
Vacuole Periphery -0.04 5.45 4.50 7.84 -1.73 -0.73 8.83 2.57 3.14 -1.96 -0.66 10.18 5.70 7.47 -2.64
ORF
Human Ortholog
Description 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YBL055C

YBL055C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybl055c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available