Standard name
Human Ortholog
Description Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.09 0.07 0.07 0.08 0.09 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.98 0.97 0.96 0.92 0.87 0.92 0.87 0.78 0.8 1.0 0.99 0.99 0.97 0.98 0.96 0.96 0.97 0.91 0.96 0.98 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0.05 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.05 0.08 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 2 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 1 0 0 0 1 1 0 1 2 1 0 1 1 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 0 1 5 6 7 18 9 11 13 18 2 6 5 14 13 13 0 0 0 0 0 0
Cytoplasm 164 233 136 130 172 194 243 175 119 139 124 160 256 328 331 343 268 193 170 233 128 165 247 234
Endoplasmic Reticulum 0 3 0 0 2 4 7 11 6 9 8 10 1 2 1 12 7 7 0 0 2 0 0 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 0 0 0 1 0 0 0
Mitochondria 0 0 1 1 1 4 10 11 6 13 17 25 0 0 0 4 0 1 0 0 0 0 0 2
Nucleus 0 2 0 0 2 1 2 1 0 1 1 1 1 0 1 2 4 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 1 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 2 4 2 0 1 4 1 1 10 4 0 0 0 8 2 2 1 1 1 0 0 1
Unique Cell Count 167 236 139 133 178 203 263 202 130 160 160 200 257 331 333 355 273 200 178 240 141 172 252 253
Labelled Cell Count 170 240 140 136 184 210 273 222 141 179 178 223 260 337 339 389 294 216 178 240 141 172 252 253


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.6 21.1 16.3 14.9 16.5 15.1 13.5 14.8 14.9 13.5 13.2 13.9 21.9 21.2 20.9 17.7 23.9 25.2 18.8 18.4 18.9
Std Deviation (1e-4) 2.7 3.7 3.0 4.1 3.9 3.8 3.5 4.0 4.2 3.5 3.3 3.8 3.7 3.8 3.7 4.3 4.8 6.5 3.3 3.7 4.2
Intensity Change (Log2) – – – -0.13 0.02 -0.11 -0.27 -0.14 -0.14 -0.28 -0.31 -0.24 0.42 0.38 0.36 0.12 0.55 0.62 0.2 0.17 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3.6 0 3.1 3.4 3.6 0 0 0 0 0 3.1
Cytoplasm -0.1 -0.6 -1.1 -2.2 -3.6 -2.3 -3.5 -5.2 -4.9 1.7 1.1 1.5 -0.7 0.2 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.0 3.6 4.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 2.1 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.1385 22.2494 20.4479 19.2885 20.0951 21.2601 14.6245 20.8145 18.7628 15.6756 16.9326 18.5436 18.577 24.3249 22.3597 23.3549 22.6007 22.6282
Actin 0.0291 0.0029 0.0142 0.0283 0.0002 0.0001 0.0246 0.0004 0.0012 0.0336 0.0007 0.0002 0.0216 0.0003 0.0017 0.0001 0.0028 0.0004
Bud 0.0003 0.0001 0.0004 0.0004 0.0001 0.0002 0.0009 0.0001 0.0002 0.0013 0.0001 0 0.0015 0.0003 0.0003 0 0.0001 0.0001
Bud Neck 0.0009 0.0002 0.0028 0.0007 0.0009 0.0009 0.0007 0.0001 0.0003 0.0006 0.0006 0.0006 0.0022 0.0001 0.0002 0.0002 0.0003 0.0006
Bud Periphery 0.0003 0 0.0004 0.0008 0 0.0001 0.0007 0 0.0001 0.0007 0 0 0.0009 0 0.0001 0 0 0
Bud Site 0.0004 0.0001 0.0018 0.0008 0 0 0.0053 0.0001 0.0002 0.0013 0.0001 0.0001 0.002 0.0002 0.0004 0 0.0002 0
Cell Periphery 0.0001 0 0.0001 0.0003 0 0 0.0005 0 0 0.0001 0 0 0.0002 0 0 0 0 0
Cytoplasm 0.8396 0.9761 0.9176 0.8601 0.9455 0.9208 0.7673 0.9932 0.9175 0.8496 0.9309 0.9569 0.8147 0.9907 0.9472 0.9868 0.9007 0.9568
Cytoplasmic Foci 0.0146 0.0005 0.0064 0.0031 0.0011 0.0004 0.0237 0.0005 0.0142 0.0288 0.024 0.005 0.0361 0.0006 0.0057 0.0003 0.0091 0.0012
Eisosomes 0.0002 0 0.0002 0.0002 0 0 0.0002 0 0 0.0001 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.008 0.0006 0.0017 0.003 0.0054 0.0027 0.0278 0.0005 0.0022 0.0034 0.0083 0.0013 0.0086 0.0002 0.0004 0.0003 0.0217 0.0009
Endosome 0.0224 0.0005 0.0084 0.0132 0.0011 0.0007 0.0565 0.0001 0.0128 0.0355 0.0084 0.0076 0.037 0.0001 0.0007 0.0001 0.0339 0.0008
Golgi 0.0049 0.0017 0.003 0.0071 0 0 0.0188 0 0.0039 0.0127 0.0007 0.0009 0.0061 0 0.0007 0 0.0025 0.0001
Lipid Particles 0.007 0.0005 0.007 0.0089 0 0 0.0127 0 0.0112 0.0073 0.0011 0.0087 0.0127 0 0.0014 0 0.0052 0.0001
Mitochondria 0.0026 0.0008 0.0078 0.0163 0.0002 0.0002 0.0092 0.0001 0.0008 0.0076 0.0002 0.0003 0.0029 0.0001 0.0147 0.0001 0.0007 0.0081
None 0.0034 0.0002 0.0014 0.0006 0.001 0.0002 0.0042 0.0005 0.0015 0.0012 0.0031 0.0006 0.0062 0.0003 0.0007 0.0002 0.0048 0.0005
Nuclear Periphery 0.0198 0.0018 0.0043 0.0067 0.0116 0.0096 0.0134 0.0006 0.0041 0.0019 0.0028 0.002 0.0083 0.0006 0.0018 0.0017 0.0081 0.0027
Nucleolus 0.0003 0 0.0002 0.0006 0 0 0.0004 0 0.0002 0.0001 0 0 0.0004 0 0 0 0.0001 0
Nucleus 0.033 0.0131 0.0157 0.0249 0.0297 0.0614 0.0118 0.0036 0.0231 0.0065 0.0166 0.014 0.0238 0.006 0.0139 0.0099 0.006 0.0268
Peroxisomes 0.0042 0.0003 0.0036 0.0034 0 0 0.0046 0 0.0014 0.0034 0.0006 0.0008 0.0043 0 0.0086 0 0.0002 0.0001
Punctate Nuclear 0.0051 0.0001 0.0016 0.0007 0.0008 0.0003 0.0055 0.0001 0.0036 0.0011 0.0012 0.0004 0.0066 0.0001 0.0012 0.0001 0.0021 0.0005
Vacuole 0.0022 0.0004 0.001 0.0137 0.0018 0.0017 0.0087 0.0001 0.0011 0.0022 0.0006 0.0004 0.0027 0.0001 0.0002 0.0001 0.0009 0.0003
Vacuole Periphery 0.0014 0.0001 0.0006 0.0062 0.0005 0.0005 0.0025 0 0.0006 0.001 0.0001 0.0001 0.0009 0 0.0001 0 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 100.3808 107.2605 79.0717 93.2833 95.5775 90.9197 115.3621 105.2508 110.1298 106.0678
Translational Efficiency 0.686 0.6759 0.8326 0.7871 0.6672 0.7437 0.7653 0.7355 0.6829 0.5955

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
226 1339 144 1611 1559 2003 96 139 1785 3342 240 1750

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1313.25 1596.87 2131.89 2194.69 1514.96 1519.80 2346.96 2372.49 1489.42 1550.68 2217.92 2208.81
Standard Deviation 175.73 273.56 306.88 354.94 233.25 246.66 302.61 419.99 236.49 260.53 322.86 363.73
Intensity Change Log 2 — 0.282105 0.698991 0.740876 — 0.004602 0.631514 0.647122 — 0.140116 0.663239 0.691414

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000307 0.000369 0.000661 0.000458 0.000116 0.000172 0.001023 0.000312 0.000141 0.000251 0.000806 0.000446
Bud Neck 0.000603 0.000337 0.006474 0.001607 0.000135 0.000312 0.005759 0.003847 0.000194 0.000322 0.006188 0.001785
Bud Site 0.009712 0.002364 0.013707 0.004128 0.001330 0.002406 0.008105 0.020681 0.002391 0.002389 0.011466 0.005443
Cell Periphery 0.000195 0.000111 0.000783 0.000155 0.000058 0.000029 0.000758 0.001605 0.000075 0.000062 0.000773 0.000270
Cytoplasm 0.871628* 0.865898* 0.692268* 0.777482* 0.935307* 0.865286* 0.672074* 0.773376* 0.927244* 0.865531* 0.684191* 0.777156*
Cytoplasmic Foci 0.056597 0.008716 0.051941 0.006956 0.006411 0.009289 0.030072 0.027615 0.012765 0.009060 0.043193 0.008597
Eisosomes 0.000007 0.000002 0.000005 0.000002 0.000001 0.000002 0.000007 0.000006 0.000001 0.000002 0.000006 0.000002
Endoplasmic Reticulum 0.001082 0.000174 0.000497 0.000568 0.000320 0.000238 0.000989 0.003617 0.000417 0.000212 0.000694 0.000810
Endosome 0.003530 0.000508 0.005712 0.002360 0.000182 0.000724 0.006321 0.003223 0.000606 0.000637 0.005955 0.002428
Golgi 0.003296 0.000776 0.003498 0.002425 0.000783 0.000874 0.003495 0.001080 0.001101 0.000835 0.003497 0.002319
Lipid Particles 0.000734 0.000273 0.006714 0.001552 0.000163 0.000205 0.010223 0.012942 0.000235 0.000232 0.008117 0.002457
Mitochondria 0.004199 0.000090 0.000080 0.000134 0.000060 0.000168 0.000117 0.000123 0.000584 0.000137 0.000095 0.000133
Mitotic Spindle 0.000864 0.001335 0.009995 0.021997 0.000742 0.004872 0.005260 0.002413 0.000757 0.003455 0.008101 0.020442
None 0.001066 0.016384 0.002540 0.007499 0.006893 0.007809 0.002194 0.004023 0.006155 0.011245 0.002402 0.007223
Nuclear Periphery 0.000051 0.000048 0.000465 0.000222 0.000026 0.000081 0.001087 0.001053 0.000029 0.000068 0.000714 0.000288
Nuclear Periphery Foci 0.010002 0.003489 0.013193 0.004693 0.001540 0.001541 0.028543 0.008232 0.002611 0.002322 0.019333 0.004974
Nucleolus 0.000213 0.000094 0.000154 0.000161 0.000032 0.000185 0.000157 0.000097 0.000055 0.000149 0.000155 0.000156
Nucleus 0.017670 0.091594 0.076395 0.144902* 0.042798 0.093288 0.113230 0.025168 0.039616 0.092610 0.091129 0.135391*
Peroxisomes 0.000334 0.000086 0.000150 0.000126 0.000019 0.000315 0.000101 0.000233 0.000059 0.000223 0.000131 0.000134
Vacuole 0.017188 0.007039 0.113635 0.020963 0.002727 0.011232 0.109539 0.101109 0.004558 0.009552 0.111997 0.027329
Vacuole Periphery 0.000721 0.000311 0.001133 0.001610 0.000358 0.000971 0.000944 0.009244 0.000404 0.000707 0.001057 0.002216

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.51 -3.64 -0.74 0.14 2.75 -1.84 -4.76 -4.26 -3.17 2.86 -1.77 -7.02 -3.45 -1.11 3.91
Bud Neck 1.73 -7.75 -7.61 -9.54 5.98 -8.79 -7.30 -8.17 -7.69 2.79 -8.05 -10.97 -12.49 -11.24 7.70
Bud Site 4.50 -1.79 3.34 -3.95 5.28 -2.58 -6.63 -6.65 -6.15 -2.60 -0.30 -8.36 -7.16 -6.63 5.62
Cell Periphery 1.79 -6.64 -0.80 -3.21 6.64 6.31 -7.57 -3.93 -4.03 -0.86 0.80 -11.16 -8.08 -7.92 7.49
Cytoplasm 1.05 10.04 11.69 21.86 -4.29 22.87 14.92 11.95 7.79 -3.73 22.64 19.92 37.90 23.78 -7.11
Cytoplasmic Foci 8.29 0.40 8.21 -0.61 7.36 -4.08 -6.58 -7.59 -6.70 -0.87 2.34 -8.36 -0.36 -3.27 8.35
Eisosomes 4.96 -0.11 4.08 -2.60 4.67 -6.76 -8.03 -6.73 -4.96 1.92 -3.85 -9.98 -9.37 -4.46 6.84
Endoplasmic Reticulum 2.89 1.78 2.84 0.13 2.14 1.14 -2.27 -0.63 -0.74 -0.17 2.69 -1.29 1.57 -0.14 2.53
Endosome 2.14 -1.08 1.96 -0.78 4.97 -3.25 -3.50 -2.14 -1.09 2.49 -0.07 -5.88 -0.70 -0.73 5.52
Golgi 1.60 0.17 1.59 -0.05 3.96 0.26 -4.32 0.51 0.15 4.18 1.40 -4.19 1.04 -0.11 4.82
Lipid Particles 2.22 -6.55 -4.70 -6.11 4.56 -1.94 -6.25 -4.77 -4.74 -0.76 -1.22 -9.38 -7.82 -7.71 5.36
Mitochondria 0.99 0.99 0.99 -0.88 -0.73 -2.75 -3.31 0.84 2.78 2.96 0.87 0.90 0.92 1.03 0.47
Mitotic Spindle -1.05 -3.94 -4.26 -3.91 0.59 -3.85 -4.15 -1.51 1.00 0.83 -4.07 -4.98 -4.51 -2.41 0.01
None -12.58 -2.58 -13.98 8.37 -8.72 -0.23 5.91 3.53 5.03 -1.62 -4.87 5.62 0.84 8.81 -10.55
Nuclear Periphery -0.49 -6.56 -11.80 -13.97 4.08 -13.05 -5.97 -1.98 -1.52 2.55 -12.59 -8.86 -12.54 -9.44 5.79
Nuclear Periphery Foci 4.04 -1.48 4.48 1.37 5.36 -1.06 -5.47 -4.75 -4.57 3.60 -0.17 -7.63 -2.19 -2.22 6.94
Nucleolus 0.65 0.11 0.20 -2.75 0.61 -2.55 -8.38 -5.88 1.78 5.04 -2.41 -5.90 -4.30 0.00 1.04
Nucleus -16.79 -7.87 -26.50 -14.11 -7.67 -20.71 -7.44 0.50 10.43 7.01 -25.71 -9.28 -28.90 -13.07 -5.89
Peroxisomes 1.53 1.00 1.69 0.59 4.03 -2.40 -6.84 -4.63 1.29 -0.06 -2.15 -4.41 -0.71 1.95 4.22
Vacuole 3.08 -7.53 -13.48 -20.53 4.39 -14.62 -9.51 -10.56 -9.44 1.10 -12.74 -12.44 -24.49 -20.20 6.63
Vacuole Periphery 1.18 -0.40 0.66 -0.61 1.36 -1.04 -1.02 -0.89 -0.66 -0.80 -0.70 -2.02 -0.64 0.01 0.54
Standard name
Human Ortholog
Description Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ptc3

Ptc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available