Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.05 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.09 0.11 0 0 0 0 0 0
Cytoplasm 0.26 0.45 0.61 0.62 0.48 0.34 0.27 – 0.17 0.17 0.12 0.15 0.71 0.77 0.82 0.22 0.14 0.22 0.07 0.11 0.07 0.06 0.07 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.08 0.07 0.06 0 0.09 0 0.16 – 0 0.06 0 0 0.1 0.12 0.08 0.25 0.23 0.11 0.12 0.07 0 0.06 0.07 0.08
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Mitochondria 0.62 0.43 0.11 0.28 0.24 0.68 0.54 – 0.83 0.76 0.87 0.81 0.15 0.06 0.07 0.12 0.09 0 0.6 0.64 0.7 0.62 0.61 0.51
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0.05 0.13 0 0 – 0 0 0 0 0 0 0.05 0.34 0.32 0.06 0 0 0 0 0 0
SpindlePole 0.06 0 0.16 0.07 0.1 0.05 0.13 – 0.09 0.14 0.09 0.09 0 0 0 0.12 0.18 0.06 0 0 0 0.05 0 0.05
Vac/Vac Membrane 0.08 0.08 0 0 0.13 0 0.11 – 0.05 0.05 0 0.05 0.05 0.06 0 0.12 0.14 0.28 0 0.06 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 2 4 5 7 7 12 11
Bud 1 5 3 5 3 14 9 – 17 23 36 32 4 1 2 0 0 0 1 2 5 3 4 11
Bud Neck 1 0 3 2 2 1 2 – 2 2 0 4 0 0 0 0 0 0 3 4 2 4 8 7
Bud Site 0 2 0 0 0 5 1 – 2 4 10 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 6 0 1 1 1 – 1 3 0 0 1 2 1 2 2 2 1 0 0 1 1 2
Cytoplasm 59 83 127 118 112 111 108 – 61 64 57 81 185 158 221 7 3 4 19 26 19 12 23 23
Endoplasmic Reticulum 6 6 0 0 0 0 0 – 0 0 1 0 1 0 0 0 1 0 3 1 3 1 5 6
Endosome 17 13 12 7 22 6 62 – 3 21 9 8 27 25 22 8 5 2 34 15 9 12 23 21
Golgi 0 0 1 0 0 0 0 – 0 0 0 2 1 1 1 0 1 1 7 4 1 3 1 5
Mitochondria 140 79 23 53 56 223 211 – 305 285 425 423 39 13 18 4 2 0 179 152 199 124 213 144
Nucleus 2 1 0 0 1 8 9 – 8 12 5 13 1 0 0 0 0 1 0 0 0 0 1 1
Nuclear Periphery 3 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 0 0 1 1 2
Nucleolus 2 1 0 0 0 2 1 – 0 1 0 2 0 1 0 0 0 0 1 0 1 1 2 0
Peroxisomes 6 3 17 9 31 2 17 – 4 14 9 9 10 8 13 11 7 1 10 3 8 3 5 3
SpindlePole 14 4 33 14 23 17 52 – 33 51 46 46 4 3 2 4 4 1 13 5 7 9 14 13
Vac/Vac Membrane 17 14 7 6 31 13 42 – 17 19 14 25 12 13 10 4 3 5 6 13 4 8 18 16
Unique Cell Count 225 185 209 190 233 329 394 368 376 491 523 259 206 270 32 22 18 298 238 285 201 347 282
Labelled Cell Count 270 215 232 214 283 403 515 453 500 612 655 285 225 290 40 28 19 298 238 285 201 347 282


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 5.2 4.7 5.5 3.9 4.7 – 4.0 4.2 3.9 4.2 4.9 4.9 5.2 6.0 5.9 6.0 4.2 4.4 4.9
Std Deviation (1e-4) 0.8 1.0 2.0 1.5 1.9 1.7 1.6 – 2.9 1.5 1.2 2.0 1.5 1.7 1.7 1.7 1.5 2.2 1.7 1.8 2.2
Intensity Change (Log2) – – – -0.14 0.08 -0.4 -0.15 – -0.38 -0.31 -0.43 -0.33 -0.09 -0.07 -0.01 0.21 0.19 0.2 -0.31 -0.23 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0005 -1.1613 -1.0295 -1.3185 -1.1917 -1.0015 0.2348 0.3007 0.4001 3.8915 0.2178 0.5599 2.3779 3.191 3.1665 2.8292 3.0288 3.2966
Actin 0.0291 0.0076 0.0062 0.0012 0.0447 0.0115 0.0479 0.0158 0.0071 0.0366 0.0544 0.0251 0.0139 0.019 0.0093 0.0183 0.0106 0.0122
Bud 0.0022 0.0029 0.0067 0.0044 0.0052 0.0046 0.0026 0.0025 0.0049 0.0044 0.0059 0.0021 0.0013 0.0038 0.0052 0.003 0.0008 0.0016
Bud Neck 0.0123 0.0014 0.0087 0.0016 0.0045 0.0263 0.0092 0.0013 0.0026 0.002 0.0064 0.0137 0.0017 0.0027 0.0107 0.0015 0.0076 0.0134
Bud Periphery 0.0053 0.0052 0.0099 0.0171 0.0136 0.0041 0.0021 0.0083 0.006 0.0117 0.0104 0.0046 0.0036 0.0087 0.0172 0.0108 0.0018 0.0047
Bud Site 0.0157 0.0271 0.0122 0.0028 0.0134 0.0016 0.008 0.0267 0.0112 0.0028 0.0068 0.0036 0.008 0.0337 0.0226 0.0028 0.0028 0.0026
Cell Periphery 0.0018 0.0009 0.0013 0.011 0.0008 0.0009 0.002 0.0021 0.0022 0.0008 0.0038 0.001 0.0044 0.002 0.0047 0.0244 0.0008 0.003
Cytoplasm 0.079 0.1355 0.1013 0.147 0.101 0.1266 0.1364 0.1452 0.1534 0.1583 0.1988 0.1989 0.1138 0.1779 0.1542 0.1496 0.1386 0.1935
Cytoplasmic Foci 0.1701 0.151 0.1393 0.1539 0.1411 0.1806 0.2112 0.1159 0.0949 0.0519 0.1322 0.1673 0.1532 0.128 0.1113 0.1022 0.2032 0.1309
Eisosomes 0.001 0.0006 0.0007 0.0003 0.0006 0.0005 0.0008 0.0014 0.0008 0.0008 0.0021 0.0002 0.0012 0.0007 0.0013 0.0008 0.0011 0.0007
Endoplasmic Reticulum 0.0066 0.0047 0.0036 0.0033 0.0042 0.0028 0.0073 0.0054 0.0079 0.0054 0.0071 0.0026 0.0056 0.0056 0.0062 0.0046 0.0038 0.0073
Endosome 0.1424 0.1544 0.1058 0.1781 0.1975 0.1321 0.1525 0.1543 0.1104 0.0738 0.1514 0.1136 0.1513 0.1348 0.1075 0.1 0.1728 0.1159
Golgi 0.0278 0.0192 0.0261 0.0203 0.028 0.0229 0.0493 0.0394 0.0379 0.0493 0.1246 0.0296 0.0468 0.0431 0.0426 0.0234 0.041 0.0344
Lipid Particles 0.0391 0.018 0.0202 0.0167 0.0172 0.0168 0.0471 0.0099 0.0212 0.02 0.0326 0.0217 0.0209 0.0091 0.0121 0.0043 0.0163 0.0152
Mitochondria 0.0817 0.0865 0.2179 0.1875 0.129 0.1672 0.0682 0.1024 0.1098 0.1246 0.0808 0.115 0.1813 0.1297 0.1974 0.1708 0.0819 0.1967
None 0.2865 0.3186 0.2456 0.18 0.2067 0.2101 0.1283 0.3271 0.3638 0.4271 0.1275 0.2536 0.2316 0.2625 0.2435 0.2873 0.2401 0.2244
Nuclear Periphery 0.0079 0.0027 0.0033 0.0051 0.0067 0.0077 0.0178 0.0013 0.0025 0.0028 0.0017 0.0014 0.0009 0.0007 0.0011 0.0011 0.0008 0.0011
Nucleolus 0.0053 0.002 0.0027 0.0012 0.002 0.0023 0.0018 0.0012 0.0027 0.0007 0.0007 0.0008 0.0009 0.001 0.0012 0.0007 0.0007 0.0008
Nucleus 0.012 0.0033 0.0042 0.0033 0.0047 0.0057 0.005 0.0017 0.0031 0.0013 0.001 0.0013 0.0013 0.0014 0.0021 0.0047 0.0014 0.0017
Peroxisomes 0.0479 0.0239 0.0564 0.0379 0.0369 0.0474 0.061 0.0179 0.0337 0.0079 0.0389 0.0277 0.0371 0.0191 0.0304 0.0612 0.0513 0.0233
Punctate Nuclear 0.0065 0.0041 0.0076 0.0033 0.0111 0.0125 0.0136 0.001 0.0038 0.0011 0.0029 0.0014 0.0012 0.0007 0.001 0.0016 0.0015 0.0011
Vacuole 0.0162 0.0231 0.015 0.0184 0.023 0.0113 0.0218 0.0152 0.0161 0.012 0.0067 0.0098 0.0155 0.0127 0.014 0.0209 0.0187 0.011
Vacuole Periphery 0.0036 0.0073 0.0052 0.0057 0.0081 0.0047 0.0061 0.0037 0.0042 0.0046 0.0033 0.0051 0.0043 0.0028 0.0043 0.0058 0.0023 0.0044

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5835 6.7792 2.5152 6.9835 4.9162 3.7897 8.1397 8.6743 6.9428 7.6604
Translational Efficiency 1.6907 1.243 2.9655 1.0032 1.602 4.7209 1.1372 1.5545 1.7886 2.2856

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
200 648 879 1176 1724 2384 217 228 1924 3032 1096 1404

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 589.80 743.03 874.66 787.61 640.71 723.45 784.54 882.68 635.42 727.63 856.82 803.05
Standard Deviation 64.98 83.02 97.27 106.88 70.43 90.44 95.03 96.15 71.59 89.27 103.27 110.90
Intensity Change Log 2 — 0.333195 0.568496 0.417256 — 0.175222 0.292175 0.462219 — 0.253101 0.431231 0.440842

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002663 0.001346 0.001895 0.008342 0.000506 0.001193 0.003722 0.004320 0.000730 0.001225 0.002257 0.007688
Bud Neck 0.070564 0.052787 0.018260 0.025988 0.045340 0.072149 0.026592 0.017266 0.047962 0.068011 0.019909 0.024572
Bud Site 0.020542 0.045876 0.025498 0.076261 0.012635 0.038949 0.064938 0.060066 0.013457 0.040429 0.033307 0.073631
Cell Periphery 0.003003 0.000410 0.000174 0.000288 0.000186 0.000402 0.000248 0.000643 0.000479 0.000403 0.000189 0.000346
Cytoplasm 0.139337* 0.144339* 0.303194* 0.365147* 0.194934* 0.167815* 0.218700* 0.260951* 0.189155* 0.162797* 0.286465* 0.348226*
Cytoplasmic Foci 0.251275* 0.343092* 0.101902 0.125448 0.361004* 0.251364* 0.149229 0.166807 0.349597* 0.270968* 0.111272 0.132164
Eisosomes 0.001657 0.000355 0.000092 0.000211 0.000316 0.000321 0.000149 0.000353 0.000455 0.000328 0.000103 0.000234
Endoplasmic Reticulum 0.001303 0.001016 0.009083 0.003505 0.001767 0.001601 0.010862 0.006234 0.001718 0.001476 0.009435 0.003948
Endosome 0.027243 0.065348 0.017705 0.016258 0.047825 0.061036 0.029585 0.019286 0.045686 0.061958 0.020057 0.016749
Golgi 0.034444 0.051273 0.003060 0.006275 0.025066 0.036161 0.005296 0.002708 0.026041 0.039391 0.003503 0.005696
Lipid Particles 0.032959 0.008860 0.007163 0.004767 0.027760 0.018868 0.007280 0.006148 0.028300 0.016729 0.007186 0.004991
Mitochondria 0.247320* 0.107649* 0.005563 0.009861 0.107909* 0.168579* 0.021687 0.005742 0.122401* 0.155557* 0.008756 0.009192
Mitotic Spindle 0.001943 0.007917 0.033991 0.024627 0.003392 0.013312 0.020601 0.009528 0.003242 0.012159 0.031340 0.022175
None 0.001067 0.001883 0.002473 0.002310 0.001429 0.005688 0.001594 0.001046 0.001391 0.004875 0.002299 0.002105
Nuclear Periphery 0.000313 0.000180 0.003351 0.000808 0.000649 0.000458 0.001376 0.001134 0.000614 0.000399 0.002960 0.000861
Nuclear Periphery Foci 0.000157 0.000273 0.003450 0.001218 0.000272 0.000306 0.004372 0.002241 0.000260 0.000299 0.003632 0.001384
Nucleolus 0.001455 0.001795 0.003523 0.001144 0.001640 0.003450 0.003550 0.001044 0.001621 0.003096 0.003528 0.001128
Nucleus 0.063207 0.031919 0.314009* 0.113875 0.073504 0.050578 0.187212* 0.185851* 0.072434 0.046590 0.288904* 0.125563
Peroxisomes 0.078213 0.075928 0.014184 0.047208 0.038803 0.041291 0.038347 0.053867 0.042900 0.048693 0.018968 0.048289
Vacuole 0.016783 0.048494 0.129132 0.164680 0.048231 0.057862 0.200350* 0.193764 0.044962 0.055860 0.143232 0.169403
Vacuole Periphery 0.004550 0.009262 0.002300 0.001779 0.006831 0.008619 0.004310 0.001001 0.006594 0.008757 0.002698 0.001653

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.95 0.44 -2.14 -4.62 -3.95 -2.91 -5.15 -2.93 -2.09 0.54 -2.06 -5.85 -5.52 -4.97 -3.72
Bud Neck 1.72 5.38 4.92 6.93 -2.10 -7.34 4.96 9.79 16.32 2.79 -6.05 10.37 9.93 17.14 -0.91
Bud Site -4.23 -0.53 -6.43 -2.54 -7.75 -11.40 -6.21 -3.42 -0.25 1.68 -12.77 -6.06 -11.49 -4.10 -5.96
Cell Periphery 3.11 3.41 3.33 1.93 -3.62 -5.54 -2.01 -1.21 -0.20 -0.92 0.78 3.20 2.10 2.58 -2.19
Cytoplasm -0.41 -9.68 -13.82 -19.04 -5.73 3.85 -1.69 -3.69 -5.36 -1.73 4.11 -9.84 -16.60 -21.05 -6.19
Cytoplasmic Foci -5.28 10.16 9.38 23.91 -2.15 15.52 19.09 19.25 10.50 0.53 11.96 36.73 36.82 28.46 -1.45
Eisosomes 4.13 4.96 4.70 4.82 -4.87 -0.20 6.86 0.55 0.62 -1.42 3.02 8.91 6.14 5.31 -4.39
Endoplasmic Reticulum 0.79 -13.54 -5.55 -8.00 10.15 0.43 -5.89 -4.32 -4.53 2.56 0.74 -14.16 -6.09 -7.45 10.04
Endosome -7.25 2.70 3.79 12.82 2.31 -4.42 5.79 8.88 12.55 2.61 -6.05 11.65 14.81 23.48 3.46
Golgi -2.48 6.05 5.63 10.86 -1.94 -5.23 10.63 15.55 20.84 1.87 -6.44 15.56 14.03 20.76 -1.32
Lipid Particles 5.47 5.94 6.63 3.94 4.03 4.57 10.25 13.63 10.64 0.91 6.66 13.55 15.90 12.34 4.07
Mitochondria 6.27 11.54 11.42 12.99 -2.09 -8.85 13.15 20.97 31.62* 2.69 -5.15 22.95 23.36 34.07 0.46
Mitotic Spindle -3.16 -8.10 -7.40 -4.86 2.15 -6.63 -3.67 -1.87 1.05 1.80 -7.12 -8.63 -7.49 -3.45 2.41
None -0.76 -3.42 -2.46 -0.18 0.37 -5.04 -0.10 -0.80 3.34 -0.77 -4.88 -2.22 -1.78 3.75 0.02
Nuclear Periphery 1.78 -13.73 -8.17 -12.13 10.56 1.62 -2.91 -4.97 -5.95 -0.75 2.15 -12.44 -5.82 -8.66 9.91
Nuclear Periphery Foci -1.97 -11.71 -9.49 -8.41 7.31 -0.88 -3.19 -6.11 -5.98 0.98 -1.12 -10.63 -10.76 -10.09 6.27
Nucleolus -0.37 -2.77 0.44 1.11 4.25 -4.92 -1.82 1.72 5.63 2.31 -4.67 -4.06 1.65 5.58 4.79
Nucleus 3.10 -18.90 -6.10 -15.64 17.52 5.62 -7.49 -7.63 -9.28 0.08 6.96 -24.99 -11.50 -17.28 15.91
Peroxisomes 0.24 7.17 4.49 6.75 -6.34 -0.98 0.36 0.33 0.70 0.03 -2.17 9.31 2.00 3.80 -5.35
Vacuole -7.99 -21.95 -28.88 -23.20 -7.69 -3.17 -12.06 -13.13 -12.58 -2.21 -4.13 -21.06 -28.27 -26.63 -7.42
Vacuole Periphery -3.21 2.83 3.82 6.42 1.73 -2.80 4.11 12.50 17.04 4.40 -3.56 8.01 11.70 16.29 3.17
Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (44%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Iai11

Iai11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Iai11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available