Standard name | |
---|---|
Human Ortholog | |
Description | Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 24 | 27 | 5 | 2 | 7 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 21 | 41 | 16 | 18 | 16 | 13 | 37 | 89 | 22 | 15 | 29 | 26 |
Bud | 16 | 19 | 2 | 0 | 2 | 1 | 5 | 3 | 0 | 3 | 9 | 3 | 6 | 7 | 7 | 17 | 18 | 12 | 13 | 43 | 8 | 9 | 15 | 16 |
Bud Neck | 8 | 19 | 21 | 4 | 22 | 3 | 12 | 11 | 14 | 14 | 3 | 5 | 21 | 53 | 26 | 7 | 24 | 11 | 54 | 143 | 39 | 16 | 20 | 23 |
Bud Site | 2 | 8 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 6 | 2 | 11 | 29 | 15 | – | – | – | – | – | – |
Cell Periphery | 13 | 35 | 8 | 6 | 3 | 5 | 4 | 0 | 3 | 3 | 2 | 2 | 13 | 16 | 18 | 15 | 14 | 11 | 16 | 58 | 17 | 15 | 33 | 46 |
Cytoplasm | 217 | 450 | 201 | 115 | 230 | 224 | 320 | 323 | 282 | 222 | 249 | 239 | 331 | 386 | 451 | 110 | 109 | 119 | 117 | 197 | 72 | 239 | 317 | 338 |
Endoplasmic Reticulum | 1 | 8 | 2 | 0 | 0 | 1 | 0 | 2 | 6 | 6 | 0 | 0 | 2 | 1 | 0 | 11 | 17 | 16 | 7 | 10 | 2 | 2 | 4 | 4 |
Endosome | 1 | 7 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 3 | 5 | 18 | 3 | 12 | 15 | 4 | 14 | 3 | 4 | 6 | 3 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 1 | 4 |
Mitochondria | 8 | 5 | 0 | 0 | 0 | 22 | 43 | 10 | 27 | 23 | 109 | 61 | 1 | 1 | 0 | 6 | 8 | 7 | 12 | 31 | 32 | 14 | 18 | 15 |
Nucleus | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 7 | 3 | 10 | 14 | 8 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 5 | 15 | 5 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 3 | 13 | 7 | 30 | 25 | 1 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 17 | 5 | 3 | 5 | 5 | 6 | 2 | 4 | 3 | 5 | 11 | 46 | 65 | 75 | 63 | 155 | 123 | 1 | 7 | 2 | 1 | 12 | 9 |
Vac/Vac Membrane | 0 | 8 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 3 | 5 | 3 | 18 | 7 | 3 | 5 | 2 |
Unique Cell Count | 251 | 530 | 216 | 123 | 243 | 242 | 357 | 341 | 315 | 255 | 314 | 287 | 401 | 519 | 569 | 210 | 322 | 286 | 277 | 630 | 217 | 335 | 482 | 510 |
Labelled Cell Count | 296 | 613 | 248 | 132 | 271 | 266 | 392 | 358 | 340 | 278 | 381 | 326 | 453 | 593 | 631 | 286 | 466 | 385 | 277 | 630 | 217 | 335 | 482 | 510 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 5.1 | 5.5 | 5.1 | 5.2 | 4.3 | 4.1 | 4.5 | 3.7 | 3.8 | 3.4 | 3.6 | 5.8 | 6.0 | 5.7 | 6.7 | 6.9 | 6.5 | 5.3 | 5.5 | 5.8 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.0 | 1.4 | 1.2 | 1.3 | 1.1 | 1.3 | 0.7 | 0.7 | 0.9 | 1.0 | 1.5 | 1.6 | 1.5 | 1.3 | 1.3 | 1.3 | 1.4 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.08 | -0.36 | -0.42 | -0.29 | -0.58 | -0.53 | -0.71 | -0.62 | 0.07 | 0.12 | 0.06 | 0.3 | 0.34 | 0.24 | -0.05 | 0.0 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 2.8 | 0.4 | 2.9 | 1.6 | 1.3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 2.8 | 2.2 |
Bud Neck | -2.2 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | 0.2 | -2.7 | -2.7 | -0.9 | -2.7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 4.2 | 3.0 |
Cell Periphery | 0.5 | -1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | -0.4 | -0.4 | 1.6 | 0.4 | 0.1 |
Cytoplasm | 0.2 | 0.7 | -0.2 | -1.4 | 0.8 | -1.4 | -2.1 | -4.3 | -3.3 | -3.6 | -5.8 | -4.6 | -9.5 | -13.6 | -11.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.7 | 2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.3 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 4.5 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 4.3 | 4.5 | 7.8 | 11.4 | 10.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7343 | -0.6849 | -0.7205 | -0.8893 | -0.9441 | -0.7549 | -0.2166 | -0.6333 | -0.5029 | -0.3803 | 0.0643 | -0.3127 | -0.0177 | -0.2767 | -0.4782 | 0.6836 | -0.1683 | -0.3105 |
Actin | 0.0206 | 0.0017 | 0.0099 | 0.001 | 0.0063 | 0.0079 | 0.0249 | 0.0006 | 0.0028 | 0.009 | 0.0504 | 0.0127 | 0.0264 | 0.001 | 0.0139 | 0.0309 | 0.0085 | 0.0131 |
Bud | 0.0011 | 0.0012 | 0.0023 | 0.0004 | 0.0009 | 0.0005 | 0.0006 | 0.0005 | 0.0006 | 0.0366 | 0.0001 | 0.0036 | 0.0026 | 0.0004 | 0.0018 | 0.0018 | 0.0003 | 0.0023 |
Bud Neck | 0.0093 | 0.0014 | 0.0113 | 0.0147 | 0.0049 | 0.0103 | 0.0014 | 0.0007 | 0.007 | 0.0008 | 0.0002 | 0.0307 | 0.0037 | 0.0016 | 0.0588 | 0.0121 | 0.0017 | 0.0151 |
Bud Periphery | 0.0022 | 0.0014 | 0.0037 | 0.004 | 0.002 | 0.0023 | 0.001 | 0.0008 | 0.0015 | 0.0146 | 0.0003 | 0.0161 | 0.0053 | 0.0006 | 0.0038 | 0.0049 | 0.0006 | 0.0076 |
Bud Site | 0.0207 | 0.0165 | 0.0236 | 0.0016 | 0.0033 | 0.0002 | 0.0063 | 0.0184 | 0.0104 | 0.0028 | 0.0002 | 0.0011 | 0.0238 | 0.0241 | 0.0493 | 0.006 | 0.0021 | 0.0006 |
Cell Periphery | 0.0025 | 0.0008 | 0.0014 | 0.0034 | 0.0005 | 0.0002 | 0.0011 | 0.0023 | 0.0024 | 0.0006 | 0.0004 | 0.0012 | 0.0035 | 0.0025 | 0.0031 | 0.0009 | 0.0006 | 0.0011 |
Cytoplasm | 0.073 | 0.0509 | 0.0903 | 0.088 | 0.0989 | 0.0915 | 0.0623 | 0.0562 | 0.0665 | 0.0363 | 0.0505 | 0.0963 | 0.1892 | 0.1941 | 0.1881 | 0.13 | 0.1169 | 0.2531 |
Cytoplasmic Foci | 0.0286 | 0.0185 | 0.0117 | 0.0058 | 0.0113 | 0.0061 | 0.0341 | 0.0102 | 0.0063 | 0.0123 | 0.0207 | 0.0214 | 0.022 | 0.0083 | 0.0147 | 0.0125 | 0.0414 | 0.0128 |
Eisosomes | 0.0009 | 0.0007 | 0.0008 | 0.0004 | 0.0004 | 0.0004 | 0.0007 | 0.0006 | 0.0007 | 0.0003 | 0.0002 | 0.0005 | 0.0007 | 0.0004 | 0.0006 | 0.0004 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0033 | 0.0012 | 0.0017 | 0.0006 | 0.0014 | 0.0013 | 0.0047 | 0.001 | 0.0019 | 0.0007 | 0.0004 | 0.0013 | 0.0044 | 0.001 | 0.0016 | 0.0018 | 0.0017 | 0.0009 |
Endosome | 0.0068 | 0.0048 | 0.002 | 0.0004 | 0.0101 | 0.0039 | 0.0148 | 0.0002 | 0.0013 | 0.0115 | 0.0014 | 0.0047 | 0.0135 | 0.0028 | 0.0023 | 0.0209 | 0.0169 | 0.0014 |
Golgi | 0.0025 | 0.0004 | 0.0007 | 0.0001 | 0.0026 | 0.0016 | 0.0063 | 0.0001 | 0.0007 | 0.0191 | 0.0022 | 0.0023 | 0.0045 | 0.0004 | 0.001 | 0.0063 | 0.0058 | 0.0014 |
Lipid Particles | 0.0066 | 0.0013 | 0.0012 | 0.0004 | 0.0302 | 0.0032 | 0.0158 | 0.0007 | 0.0076 | 0.0086 | 0.0019 | 0.0057 | 0.0056 | 0.0006 | 0.0009 | 0.008 | 0.0241 | 0.0041 |
Mitochondria | 0.0023 | 0.0005 | 0.0044 | 0.0003 | 0.0025 | 0.0035 | 0.0051 | 0.0002 | 0.0076 | 0.0115 | 0.0003 | 0.0018 | 0.0049 | 0.0003 | 0.0008 | 0.0391 | 0.002 | 0.0007 |
None | 0.802 | 0.8888 | 0.8238 | 0.8724 | 0.8142 | 0.859 | 0.7654 | 0.9039 | 0.8725 | 0.7999 | 0.8475 | 0.7793 | 0.6582 | 0.7543 | 0.6454 | 0.6609 | 0.7502 | 0.6775 |
Nuclear Periphery | 0.0027 | 0.0006 | 0.0005 | 0.0003 | 0.0007 | 0.0023 | 0.0174 | 0.0001 | 0.0003 | 0.0015 | 0.0002 | 0.0027 | 0.0101 | 0.0002 | 0.0015 | 0.0057 | 0.0064 | 0.0007 |
Nucleolus | 0.0012 | 0.001 | 0.0011 | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0.0009 | 0.0023 | 0.0001 | 0.0008 | 0.0011 | 0.0009 | 0.0008 | 0.0119 | 0.0004 | 0.0003 |
Nucleus | 0.0014 | 0.0029 | 0.0022 | 0.0011 | 0.0008 | 0.0009 | 0.0037 | 0.0004 | 0.001 | 0.0079 | 0.0001 | 0.0017 | 0.0044 | 0.002 | 0.0022 | 0.0202 | 0.0008 | 0.001 |
Peroxisomes | 0.005 | 0.0011 | 0.0037 | 0.0001 | 0.0048 | 0.0016 | 0.0243 | 0.0008 | 0.0049 | 0.0094 | 0.0175 | 0.0031 | 0.0087 | 0.0005 | 0.0048 | 0.0039 | 0.0082 | 0.0026 |
Punctate Nuclear | 0.0053 | 0.0029 | 0.0026 | 0.0036 | 0.0028 | 0.0021 | 0.007 | 0.0015 | 0.0022 | 0.0127 | 0.0049 | 0.0119 | 0.0044 | 0.0029 | 0.003 | 0.0144 | 0.0097 | 0.0027 |
Vacuole | 0.0018 | 0.0015 | 0.0008 | 0.0006 | 0.0009 | 0.0005 | 0.0015 | 0.0005 | 0.0009 | 0.0012 | 0.0002 | 0.0007 | 0.0022 | 0.0011 | 0.0013 | 0.0052 | 0.0007 | 0.0006 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0004 | 0.0009 | 0 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0023 | 0.0006 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.6824 | 6.6905 | 7.0924 | 8.357 | 6.9313 | 4.8087 | 8.9392 | 9.7838 | 9.8737 | 9.7771 |
Translational Efficiency | 0.8265 | 0.9305 | 1.0866 | 0.875 | 0.7036 | 1.1924 | 0.686 | 0.6479 | 0.7802 | 0.5496 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1737 | 1456 | 321 | 1308 | 1236 | 2685 | 199 | 1904 | 2973 | 4141 | 520 | 3212 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 667.04 | 670.45 | 848.19 | 787.65 | 625.44 | 706.58 | 905.66 | 818.06 | 649.75 | 693.88 | 870.18 | 805.68 |
Standard Deviation | 66.19 | 93.48 | 86.24 | 100.14 | 75.64 | 92.15 | 94.11 | 103.34 | 73.20 | 94.21 | 93.60 | 103.14 |
Intensity Change Log 2 | — | 0.007356 | 0.346614 | 0.239781 | — | 0.175981 | 0.534098 | 0.387335 | — | 0.091418 | 0.440383 | 0.313069 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000468 | 0.001235 | 0.000438 | 0.005769 | 0.000363 | 0.001756 | 0.000694 | 0.006076 | 0.000424 | 0.001573 | 0.000536 | 0.005951 |
Bud Neck | 0.113058 | 0.044594 | 0.028370 | 0.074305 | 0.032873 | 0.087358 | 0.005688 | 0.148053 | 0.079722 | 0.072322 | 0.019690 | 0.118021 |
Bud Site | 0.021727 | 0.028140 | 0.020069 | 0.164897* | 0.009385 | 0.051227 | 0.021901 | 0.193024* | 0.016596 | 0.043110 | 0.020770 | 0.181570* |
Cell Periphery | 0.002077 | 0.000301 | 0.000414 | 0.001032 | 0.000400 | 0.000407 | 0.000833 | 0.001406 | 0.001380 | 0.000370 | 0.000574 | 0.001253 |
Cytoplasm | 0.520382* | 0.431543* | 0.242376* | 0.365917* | 0.502413* | 0.432442* | 0.266676* | 0.352313* | 0.512912* | 0.432126* | 0.251676* | 0.357853* |
Cytoplasmic Foci | 0.118910 | 0.250269* | 0.056170 | 0.052156 | 0.220015* | 0.178652 | 0.044462 | 0.027640 | 0.160944 | 0.203833* | 0.051689 | 0.037623 |
Eisosomes | 0.003036 | 0.000484 | 0.000172 | 0.000253 | 0.000599 | 0.000701 | 0.000281 | 0.000288 | 0.002023 | 0.000625 | 0.000213 | 0.000274 |
Endoplasmic Reticulum | 0.000692 | 0.001128 | 0.003979 | 0.002486 | 0.000753 | 0.000922 | 0.006097 | 0.001225 | 0.000717 | 0.000995 | 0.004790 | 0.001739 |
Endosome | 0.006634 | 0.023974 | 0.009502 | 0.005090 | 0.011100 | 0.020264 | 0.006676 | 0.005143 | 0.008491 | 0.021568 | 0.008421 | 0.005121 |
Golgi | 0.002598 | 0.006174 | 0.000300 | 0.000720 | 0.002395 | 0.007458 | 0.000153 | 0.001609 | 0.002514 | 0.007006 | 0.000244 | 0.001247 |
Lipid Particles | 0.007778 | 0.010572 | 0.005350 | 0.001716 | 0.010716 | 0.006016 | 0.005582 | 0.001149 | 0.009000 | 0.007618 | 0.005439 | 0.001380 |
Mitochondria | 0.002408 | 0.006118 | 0.000424 | 0.001095 | 0.002075 | 0.016943 | 0.000223 | 0.004085 | 0.002270 | 0.013137 | 0.000347 | 0.002867 |
Mitotic Spindle | 0.000542 | 0.001458 | 0.001459 | 0.012301 | 0.001039 | 0.010937 | 0.000573 | 0.035860 | 0.000749 | 0.007604 | 0.001120 | 0.026266 |
None | 0.005243 | 0.003624 | 0.003019 | 0.001606 | 0.004974 | 0.002111 | 0.001301 | 0.000984 | 0.005131 | 0.002643 | 0.002362 | 0.001237 |
Nuclear Periphery | 0.000132 | 0.000279 | 0.001967 | 0.000401 | 0.000439 | 0.000202 | 0.001413 | 0.000321 | 0.000260 | 0.000229 | 0.001755 | 0.000353 |
Nuclear Periphery Foci | 0.000329 | 0.000847 | 0.001684 | 0.001509 | 0.000539 | 0.000214 | 0.003809 | 0.000486 | 0.000416 | 0.000437 | 0.002497 | 0.000902 |
Nucleolus | 0.000808 | 0.001219 | 0.001572 | 0.000259 | 0.000902 | 0.000935 | 0.000508 | 0.000394 | 0.000847 | 0.001035 | 0.001165 | 0.000339 |
Nucleus | 0.143455* | 0.093642 | 0.500720* | 0.140188* | 0.146252* | 0.079179 | 0.382153* | 0.089139 | 0.144618* | 0.084265 | 0.455345* | 0.109927 |
Peroxisomes | 0.003748 | 0.016735 | 0.006032 | 0.018365 | 0.005057 | 0.017382 | 0.001546 | 0.011626 | 0.004292 | 0.017155 | 0.004315 | 0.014370 |
Vacuole | 0.045529 | 0.075399 | 0.115433 | 0.149277 | 0.046903 | 0.082360 | 0.249252* | 0.117921 | 0.046101 | 0.079912 | 0.166645 | 0.130690 |
Vacuole Periphery | 0.000443 | 0.002265 | 0.000552 | 0.000657 | 0.000810 | 0.002534 | 0.000179 | 0.001259 | 0.000596 | 0.002440 | 0.000409 | 0.001014 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.94 | -1.02 | -8.51 | -6.84 | -8.25 | -6.83 | -3.52 | -7.15 | -4.94 | -6.57 | -8.06 | -2.87 | -10.28 | -7.48 | -9.76 |
Bud Neck | 11.30 | 12.05 | 8.87 | -2.43 | -5.02 | -12.69 | 7.73 | -14.61 | -5.01 | -21.90 | 1.92 | 13.24 | -2.26 | -4.82 | -16.32 |
Bud Site | -2.28 | 0.67 | -16.92 | -15.67 | -15.16 | -16.38 | -1.57 | -27.47* | -18.70 | -17.28 | -13.37 | -0.67 | -31.21 | -23.92 | -24.36 |
Cell Periphery | 15.17 | 13.89 | 9.17 | -7.62 | -5.71 | -0.04 | -3.35 | -4.30 | -4.20 | -2.37 | 13.49 | 11.02 | 3.19 | -6.13 | -4.58 |
Cytoplasm | 9.98 | 22.54 | 19.73 | 9.39 | -8.00 | 8.05 | 18.44 | 15.90 | 9.47 | -8.80 | 13.31 | 29.03 | 25.47 | 13.32 | -12.11 |
Cytoplasmic Foci | -20.94 | 10.59 | 15.85 | 33.01 | 0.64 | 6.40 | 25.58 | 33.78 | 36.36 | 1.46 | -9.87 | 23.59 | 35.56 | 47.82 | 2.33 |
Eisosomes | 14.05 | 17.07 | 16.75 | 3.11 | -2.72 | -1.40 | 6.99 | 7.40 | 7.48 | 0.57 | 12.75 | 17.70 | 17.38 | 7.95 | -1.75 |
Endoplasmic Reticulum | -3.58 | -7.92 | -12.72 | -10.68 | 3.44 | -1.05 | -10.02 | -4.24 | -3.64 | 8.98 | -2.72 | -12.01 | -12.21 | -10.07 | 8.55 |
Endosome | -11.17 | -2.18 | 3.23 | 12.73 | 3.78 | -6.92 | 2.17 | 6.05 | 16.02 | 2.23 | -14.23 | -0.43 | 6.58 | 20.52 | 4.34 |
Golgi | -6.65 | 10.36 | 9.52 | 11.20 | -1.63 | -9.64 | 9.09 | 3.31 | 11.67 | -3.74 | -11.78 | 13.65 | 7.05 | 15.83 | -3.73 |
Lipid Particles | -2.63 | 1.76 | 7.29 | 10.97 | 6.93 | 4.86 | 4.90 | 10.13 | 12.53 | 8.51 | 2.05 | 4.36 | 12.34 | 16.31 | 10.58 |
Mitochondria | -5.32 | 4.95 | 3.59 | 8.95 | -3.12 | -11.71 | 4.31 | -1.29 | 10.18 | -4.38 | -12.68 | 6.52 | 0.18 | 12.23 | -4.68 |
Mitotic Spindle | -1.70 | -1.91 | -5.93 | -5.49 | -5.07 | -6.99 | -0.41 | -10.84 | -7.08 | -10.57 | -7.41 | -1.78 | -12.37 | -8.35 | -11.62 |
None | 1.63 | 2.63 | 4.74 | 3.42 | 3.33 | 2.44 | 3.52 | 3.34 | 3.94 | -1.48 | 3.48 | 4.20 | 5.80 | 5.00 | 3.17 |
Nuclear Periphery | -5.38 | -16.66 | -16.49 | -9.20 | 12.17 | 1.89 | -7.59 | -1.11 | -10.48 | 9.97 | 0.49 | -17.23 | -6.31 | -14.21 | 15.41 |
Nuclear Periphery Foci | -2.04 | -4.54 | -6.01 | -2.99 | 1.13 | 5.10 | -2.25 | -0.52 | -6.43 | 2.23 | -0.20 | -3.38 | -6.14 | -4.85 | 2.73 |
Nucleolus | -1.92 | -1.64 | 2.20 | 7.24 | 2.09 | -0.34 | 0.43 | 2.94 | 3.21 | 0.61 | -1.46 | -1.50 | 2.90 | 6.32 | 2.11 |
Nucleus | 10.57 | -20.19 | -2.70 | -10.69 | 18.73 | 13.78 | -10.02 | 6.81 | -6.57 | 12.04 | 18.43 | -21.64 | 3.75 | -12.33 | 22.60 |
Peroxisomes | -8.79 | -1.50 | -5.33 | 1.14 | -2.11 | -10.95 | 4.59 | -3.97 | 4.32 | -6.65 | -14.90 | -0.60 | -6.87 | 3.87 | -3.59 |
Vacuole | -9.67 | -9.93 | -25.41 | -19.97 | -6.47 | -11.77 | -14.96 | -23.25 | -16.10 | 8.42 | -15.77 | -16.42 | -34.38 | -25.13 | 2.47 |
Vacuole Periphery | -5.79 | -0.19 | 0.01 | 5.01 | 0.15 | -5.08 | 2.77 | -0.21 | 4.60 | -2.76 | -8.37 | 1.44 | -0.53 | 6.68 | -1.61 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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