Standard name
Human Ortholog
Description Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.11 0.1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.09 0.17 0.07 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.79 0.62 0.67 0.52 0.71 0.73 0.62 0.62 0.54 0.49 0.52 0.86 0.91 0.83 0.94 0.92 0.91 0.47 0.49 0.43 0.25 0.35 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.14 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.12 0.14 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.14 0.21 0.46 0.41 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.08 0 0.08 0.12 0.07 0 0 0 0 0 0 0.12 0.11 0.07 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0.34 0.13 0.28 0.26 0.42 0.18 0.2 0.2 0.16 0.12 0.09 0.07 0.08 0.09 0.19 0 0.05 0.1 0.15 0.14 0.12 0.09 0.27 0.54
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 15 5 4 2 2 0 2 0 0 0 1 6 2 2 9 6 4 3 9 10 41 33 11
Bud 5 5 4 1 4 6 6 7 8 8 12 8 1 2 0 1 0 0 19 19 31 35 20 16
Bud Neck 0 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 23 28 24 61 36 10
Bud Site 3 4 9 5 5 9 5 6 4 3 3 6 2 0 0 3 4 2 – – – – – –
Cell Periphery 0 0 0 4 0 1 1 0 0 0 2 1 0 0 0 3 0 1 0 0 0 1 1 0
Cytoplasm 236 229 162 169 160 260 314 297 178 231 149 180 137 209 327 266 237 157 154 158 121 87 185 122
Endoplasmic Reticulum 2 4 1 5 0 1 0 2 0 0 1 2 4 3 2 9 13 3 0 0 3 14 18 11
Endosome 4 0 11 13 42 25 18 15 9 2 1 4 0 0 3 3 0 0 15 13 16 42 75 55
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 5 0 0 1 0 2
Mitochondria 1 13 3 4 2 13 34 67 59 197 125 143 0 0 0 5 9 2 2 3 1 10 6 3
Nucleus 5 8 9 5 6 14 18 38 9 33 36 25 6 7 4 0 2 5 40 35 20 4 1 4
Nuclear Periphery 0 2 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 2 0 1 2 4 4
Nucleolus 0 0 0 2 2 2 2 4 3 0 3 3 0 0 0 0 0 0 0 0 0 1 1 1
Peroxisomes 4 0 13 5 15 6 3 4 6 1 0 3 0 0 0 3 1 1 0 0 0 0 0 0
SpindlePole 2 3 10 5 8 5 5 7 6 4 1 4 3 1 1 3 4 1 9 6 14 10 3 2
Vac/Vac Membrane 104 39 72 66 129 66 87 94 47 52 27 25 12 21 75 5 14 18 48 45 33 33 142 297
Unique Cell Count 309 290 261 253 310 367 430 478 287 428 302 347 159 230 396 282 258 173 329 325 285 355 537 551
Labelled Cell Count 380 322 300 289 375 410 493 543 330 533 360 406 171 245 414 312 290 194 329 325 285 355 537 551


Bud Neck, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 5.6 6.5 6.5 6.6 5.3 5.0 4.7 4.5 4.0 4.2 4.2 6.4 6.8 6.9 5.6 6.1 7.7 8.8 8.8 9.0
Std Deviation (1e-4) 0.9 0.8 1.4 1.5 1.6 1.3 1.2 1.0 0.9 0.7 1.1 0.9 0.6 0.8 0.9 1.3 1.1 1.8 1.5 1.5 1.5
Intensity Change (Log2) – – – -0.01 0.01 -0.3 -0.38 -0.46 -0.54 -0.71 -0.64 -0.62 -0.03 0.05 0.08 -0.23 -0.11 0.24 0.43 0.43 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.9 0.3 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.0 -1.4 -0.7 -2.1 0 0 0 0 0 0 0 0 -1.9 -1.4 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 -2.5 2.3 3.0 0 0 -2.1 -3.0 -2.5 5.3 7.4 5.9 9.2 8.0 6.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0
Endosome 0.5 3.8 1.4 0 0 0 0 0 0 0 -3.1 -3.0 -2.3 -3.3 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.9 3.8 0 0 0 0 0 0 0 0 0 1.8 0
Nucleus -1.0 -1.1 0.2 0.5 2.4 -0.2 2.3 3.7 2.0 0.2 -0.3 -2.2 0 -2.1 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -1.9 -0.1 -2.4 -3.6 0 0 0 0 0 0 -3.4 -4.5 -2.7 -3.2 -2.5
SpindlePole -1.2 -0.9 -2.0 -2.3 0 0 0 0 0 0 -2.5 0 -2.1 -1.6 0
Vacuole -0.4 3.5 -2.9 -2.2 -2.5 -3.2 -5.1 -5.8 -6.8 -5.0 -5.2 -2.6 -8.6 -6.8 -4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0103 0.0118 0.0127 0.0107 0.0011 0.0127 0.0106 0.0032 0.0027 0.0043 0.001 0.0034
Bud – – – – – – 0.0048 0.0028 0.0037 0.0007 0.0003 0.0006 0.0065 0.0049 0.0009 0.0047 0.0044 0.0008
Bud Neck – – – – – – 0.0669 0.0172 0.0127 0.0152 0.0374 0.4031 0.0256 0.0237 0.0208 0.006 0.0123 0.4682
Bud Periphery – – – – – – 0.0041 0.001 0.0024 0.0004 0.0002 0.0006 0.0049 0.0029 0.0006 0.005 0.003 0.0007
Bud Site – – – – – – 0.0727 0.1423 0.031 0.0101 0.003 0.0022 0.097 0.1548 0.0331 0.012 0.012 0.0046
Cell Periphery – – – – – – 0.0008 0.0004 0.0004 0.0004 0.0001 0.0003 0.0008 0.0006 0.0002 0.0001 0.0001 0.0002
Cytoplasm – – – – – – 0.6509 0.5553 0.6108 0.3418 0.4554 0.4243 0.6703 0.5693 0.6042 0.523 0.4995 0.3951
Cytoplasmic Foci – – – – – – 0.0299 0.0084 0.0209 0.0156 0.0064 0.0172 0.0153 0.0093 0.0059 0.0129 0.0083 0.0206
Eisosomes – – – – – – 0.0001 0 0.0001 0.0002 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum – – – – – – 0.0131 0.0043 0.0067 0.0025 0.004 0.0039 0.0074 0.0049 0.0048 0.0035 0.0045 0.0025
Endosome – – – – – – 0.0224 0.0023 0.0087 0.0052 0.0029 0.0025 0.0035 0.0057 0.0023 0.0121 0.0045 0.0032
Golgi – – – – – – 0.0058 0.0005 0.0037 0.0019 0.0008 0.0023 0.001 0.0005 0.0003 0.0004 0.0003 0.0007
Lipid Particles – – – – – – 0.0054 0.0002 0.0038 0.0043 0.0011 0.0023 0.0005 0.0003 0.0001 0.0002 0.0002 0.0003
Mitochondria – – – – – – 0.005 0.0003 0.009 0.0007 0.0005 0.0021 0.0006 0.0003 0.0004 0.0006 0.0005 0.0002
None – – – – – – 0.047 0.0062 0.0317 0.021 0.0583 0.0373 0.0539 0.0201 0.0393 0.04 0.0592 0.018
Nuclear Periphery – – – – – – 0.0065 0.0074 0.012 0.0101 0.009 0.0024 0.0066 0.0045 0.008 0.009 0.0109 0.0021
Nucleolus – – – – – – 0.0005 0.0007 0.0009 0.0028 0.001 0.0013 0.0006 0.0013 0.0009 0.0014 0.0012 0.0005
Nucleus – – – – – – 0.0292 0.2253 0.1972 0.5196 0.4068 0.0686 0.0749 0.1786 0.2579 0.3317 0.3364 0.0557
Peroxisomes – – – – – – 0.0033 0.0009 0.0068 0.0035 0.0025 0.0046 0.0034 0.0008 0.0005 0.0028 0.0003 0.0019
Punctate Nuclear – – – – – – 0.0147 0.0105 0.0224 0.0314 0.0076 0.0106 0.0133 0.0129 0.0154 0.024 0.0389 0.0206
Vacuole – – – – – – 0.0054 0.0022 0.002 0.0017 0.0014 0.0012 0.0032 0.0013 0.0014 0.0053 0.0023 0.0008
Vacuole Periphery – – – – – – 0.0013 0.0001 0.0004 0.0002 0.0002 0.0001 0.0003 0.0002 0.0002 0.0008 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.3 20.2842 19.2522 31.5266 34.2088 22.6782 24.0865 18.1883 23.1052 38.0984
Translational Efficiency 1.1633 1.0178 1.0577 0.6403 0.9249 1.6601 0.8567 0.8439 0.8277 0.9583

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
668 1207 668 1413 1844 1478 1688 1533 2512 2685 2356 2946

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 918.85 836.74 1030.74 1048.13 771.58 856.15 945.97 1022.78 810.74 847.42 970.00 1034.94
Standard Deviation 125.30 126.55 115.34 146.94 128.23 107.85 156.93 140.71 143.11 117.03 151.25 144.29
Intensity Change Log 2 — -0.135050 0.165779 0.189916 — 0.150048 0.293979 0.406608 — 0.002098 0.225711 0.292875

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002719 0.000528 0.000931 0.004955 0.000343 0.000715 0.001113 0.002663 0.000975 0.000631 0.001062 0.003762
Bud Neck 0.100443 0.072866 0.068190 0.089247 0.097490 0.096425 0.145842 0.107610 0.098275 0.085834 0.123825 0.098803
Bud Site 0.180354* 0.081938 0.079273 0.219742* 0.059409 0.073875 0.203278* 0.226092* 0.091571 0.077500 0.168119* 0.223046*
Cell Periphery 0.000737 0.000236 0.000229 0.000352 0.000312 0.000475 0.000275 0.000339 0.000425 0.000367 0.000262 0.000346
Cytoplasm 0.298036* 0.429265* 0.233781* 0.349428* 0.419103* 0.388772* 0.292038* 0.347178* 0.386909* 0.406975* 0.275520* 0.348257*
Cytoplasmic Foci 0.027831 0.052819 0.005589 0.013909 0.053822 0.097867 0.005013 0.013288 0.046911 0.077617 0.005176 0.013586
Eisosomes 0.000135 0.000059 0.000050 0.000070 0.000069 0.000123 0.000057 0.000078 0.000087 0.000094 0.000055 0.000074
Endoplasmic Reticulum 0.003771 0.001943 0.006968 0.002290 0.000883 0.001229 0.002391 0.001870 0.001651 0.001550 0.003689 0.002072
Endosome 0.006533 0.008209 0.002731 0.007893 0.003931 0.010762 0.002539 0.008933 0.004623 0.009615 0.002593 0.008434
Golgi 0.004485 0.001989 0.000145 0.007523 0.000894 0.002816 0.000464 0.009293 0.001849 0.002444 0.000373 0.008444
Lipid Particles 0.002345 0.002716 0.001282 0.000909 0.001459 0.004080 0.000612 0.000809 0.001694 0.003467 0.000802 0.000857
Mitochondria 0.003661 0.002141 0.000653 0.003082 0.002734 0.004902 0.000864 0.006613 0.002980 0.003661 0.000804 0.004919
Mitotic Spindle 0.012550 0.004724 0.002605 0.040071 0.001953 0.005236 0.008399 0.039252 0.004771 0.005006 0.006756 0.039645
None 0.002320 0.008778 0.006587 0.004099 0.008060 0.003037 0.004525 0.005159 0.006533 0.005618 0.005110 0.004651
Nuclear Periphery 0.000579 0.000442 0.001565 0.000783 0.000376 0.000472 0.000686 0.000626 0.000430 0.000459 0.000935 0.000701
Nuclear Periphery Foci 0.001359 0.002438 0.002615 0.001731 0.000676 0.000943 0.001280 0.001143 0.000857 0.001615 0.001659 0.001425
Nucleolus 0.000644 0.001601 0.000586 0.000303 0.002173 0.001347 0.000670 0.000993 0.001767 0.001461 0.000646 0.000662
Nucleus 0.213920* 0.265928* 0.519178* 0.148302* 0.307101* 0.222686* 0.284228* 0.103662 0.282322* 0.242125* 0.350844* 0.125073
Peroxisomes 0.005799 0.009728 0.001316 0.004168 0.002664 0.008926 0.003475 0.004217 0.003497 0.009287 0.002863 0.004194
Vacuole 0.131062 0.051164 0.065536 0.097178 0.036253 0.074588 0.042069 0.114603 0.061465 0.064058 0.048723 0.106245
Vacuole Periphery 0.000714 0.000486 0.000191 0.003965 0.000296 0.000726 0.000183 0.005581 0.000407 0.000618 0.000185 0.004806

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.91 1.86 -0.26 -2.74 -1.87 -2.27 -5.13 -5.23 -3.26 -1.87 0.92 -1.79 -3.24 -3.88 -2.44
Bud Neck -0.95 1.83 4.36 6.32 2.37 0.34 0.10 8.58 7.77 9.08 0.76 0.94 10.39 10.10 10.10
Bud Site 3.37 5.86 3.78 0.46 -3.12 -2.17 -10.29 -4.57 -2.37 5.52 -0.17 -5.89 -1.52 -1.38 4.61
Cell Periphery 1.82 1.77 1.75 -0.37 -0.10 -1.83 1.76 2.48 3.01 1.33 -0.08 2.46 2.77 2.78 0.96
Cytoplasm -10.76 5.54 -4.11 8.47 -11.88 2.84 14.79 7.76 4.63 -6.63 -2.88 15.68 5.86 9.14 -10.91
Cytoplasmic Foci -9.38 7.96 6.27 15.85 -4.18 -10.37 21.86 19.72 24.40 -5.47 -11.01 22.95 20.62 28.64 -6.81
Eisosomes 7.66 7.72 6.39 -0.94 -1.14 -4.53 2.15 -0.62 4.24 -2.71 -1.57 6.23 3.32 3.62 -2.57
Endoplasmic Reticulum 3.41 -5.75 2.04 -3.92 11.07 -3.08 -14.41 -11.62 -8.22 5.93 0.75 -13.02 -4.83 -8.37 11.16
Endosome -3.19 2.25 2.78 5.84 1.21 -7.86 4.41 0.82 7.81 -2.49 -8.35 4.91 2.48 9.81 -1.68
Golgi 0.50 2.27 0.52 0.06 -2.96 -2.64 1.73 -1.88 0.01 -2.66 -1.93 2.84 -1.50 0.08 -3.71
Lipid Particles -0.70 1.08 1.61 1.43 1.20 -5.08 3.72 1.33 5.49 -3.60 -3.93 3.31 1.65 4.50 -3.12
Mitochondria 0.09 1.27 0.69 0.77 -2.63 -1.43 2.15 0.05 1.61 -2.92 -0.97 2.49 0.68 1.85 -3.50
Mitotic Spindle 1.45 2.06 -3.76 -6.11 -7.00 -2.23 -4.26 -7.27 -5.66 -4.26 -0.98 -2.64 -8.90 -8.28 -6.84
None -4.20 -3.32 -6.43 2.38 -0.14 4.71 3.63 2.53 -4.50 -2.37 1.14 2.65 1.79 0.36 -1.95
Nuclear Periphery 8.72 -10.50 -9.71 -20.11 1.38 -2.89 -15.74 -16.61 -14.97 -4.59 2.81 -18.87 -22.10 -24.42 -4.65
Nuclear Periphery Foci -2.85 -5.38 -1.75 1.97 4.17 -2.07 -5.37 -5.46 -1.73 2.02 -4.14 -7.63 -5.88 1.13 3.17
Nucleolus -3.45 -1.14 1.65 4.27 3.36 1.70 3.45 3.50 3.65 0.49 0.88 3.31 3.78 5.67 2.22
Nucleus -1.43 -19.46 -0.65 1.09 22.72 9.32 -6.99 10.61 0.86 16.97 6.39 -15.69 7.89 1.16 24.35
Peroxisomes -3.54 3.25 2.52 6.16 -1.15 -5.82 1.05 2.27 7.00 0.98 -7.03 2.68 3.50 9.30 0.71
Vacuole 15.93 10.71 -0.83 -24.44 -15.29 -10.67 -11.63 -30.52 -23.32 -21.70 4.88 -0.15 -26.33 -33.58 -27.43
Vacuole Periphery -0.13 1.74 -1.38 -1.38 -2.02 -4.21 3.39 -2.67 -1.84 -2.92 -3.65 3.47 -3.10 -2.27 -3.58
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (28%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Skt5

Skt5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Skt5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available