Standard name | |
---|---|
Human Ortholog | |
Description | 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 1 | 2 | 1 | 4 | 6 | 2 | 3 | 5 | 9 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 1 | 0 | 1 | 2 | 9 | 4 | 5 | 17 | 12 | 10 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 13 | 5 | 10 | 17 | 5 | 16 | 15 | 11 | 14 | 11 | 20 | 2 | 5 | 4 | 27 | 24 | 27 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 174 | 418 | 214 | 202 | 300 | 259 | 253 | 273 | 230 | 259 | 212 | 223 | 186 | 198 | 186 | 326 | 284 | 291 | 170 | 403 | 212 | 150 | 245 | 323 |
Endoplasmic Reticulum | 1 | 8 | 2 | 3 | 2 | 7 | 7 | 9 | 8 | 0 | 8 | 5 | 3 | 0 | 1 | 13 | 10 | 6 | 4 | 6 | 10 | 13 | 13 | 15 |
Endosome | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 3 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | 1 | 0 | 1 | 19 | 71 | 143 | 119 | 158 | 188 | 199 | 140 | 1 | 0 | 0 | 3 | 2 | 10 | 0 | 1 | 2 | 3 | 6 | 9 |
Nucleus | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 3 | 2 | 2 | 4 | 1 | 3 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 1 | 9 | 3 | 23 | 11 | 21 | 21 | 10 | 21 | 8 | 23 | 0 | 1 | 1 | 38 | 21 | 10 | 1 | 8 | 2 | 6 | 5 | 7 |
Unique Cell Count | 178 | 430 | 225 | 212 | 339 | 313 | 366 | 384 | 348 | 420 | 370 | 356 | 193 | 200 | 189 | 368 | 312 | 311 | 181 | 429 | 237 | 181 | 279 | 364 |
Labelled Cell Count | 183 | 444 | 232 | 220 | 367 | 361 | 458 | 454 | 427 | 510 | 459 | 434 | 196 | 206 | 195 | 413 | 347 | 348 | 181 | 429 | 237 | 181 | 279 | 364 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.7 | 13.9 | 10.3 | 9.9 | 9.1 | 7.2 | 6.2 | 6.9 | 6.0 | 5.9 | 5.2 | 6.0 | 11.1 | 11.8 | 11.3 | 16.4 | 16.4 | 16.6 | 12.1 | 11.9 | 12.9 |
Std Deviation (1e-4) | 1.6 | 2.0 | 1.3 | 1.4 | 1.8 | 1.4 | 1.1 | 1.4 | 1.5 | 1.0 | 1.0 | 1.0 | 1.9 | 1.7 | 1.8 | 2.9 | 3.5 | 2.9 | 2.8 | 2.7 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.17 | -0.53 | -0.73 | -0.59 | -0.78 | -0.8 | -0.98 | -0.78 | 0.11 | 0.2 | 0.13 | 0.67 | 0.67 | 0.69 | 0.23 | 0.21 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.4 | 1.7 | 0 | 1.4 | 1.1 | 0.7 | 0.8 | 0.5 | 2.0 | 0 | 0 | 0 | 2.7 | 2.8 | 3.1 |
Cytoplasm | 0.1 | -2.7 | -4.3 | -7.5 | -7.1 | -8.1 | -9.2 | -9.9 | -8.8 | 0.6 | 2.3 | 1.9 | -2.7 | -1.8 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 1.8 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.6 | 7.7 | 10.8 | 9.3 | 11.9 | 11.9 | 13.5 | 10.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.7 | 1.4 | -0.3 | 0.9 | 0.8 | -0.7 | 0.6 | -1.3 | 1.3 | 0 | 0 | 0 | 2.8 | 1.4 | -0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.7823 | 11.7631 | 10.167 | 9.5273 | 8.8813 | 10.3138 | 7.7521 | 10.489 | 9.7113 | 8.9405 | 8.7738 | 9.297 | 7.9272 | 11.8307 | 10.3195 | 9.5262 | 9.5554 | 9.9038 |
Actin | 0.012 | 0.0009 | 0.011 | 0.0228 | 0.0127 | 0.0012 | 0.0062 | 0.0015 | 0.0002 | 0.0002 | 0.0034 | 0.0013 | 0.0332 | 0.0003 | 0.0254 | 0.0074 | 0.0004 | 0.0029 |
Bud | 0.0005 | 0.0003 | 0.0007 | 0.002 | 0.0003 | 0.0004 | 0.0009 | 0.0024 | 0.0004 | 0.0006 | 0.0004 | 0.0016 | 0.0011 | 0.0002 | 0.0007 | 0.0006 | 0.0004 | 0.0003 |
Bud Neck | 0.0006 | 0.0003 | 0.0007 | 0.0008 | 0.0013 | 0.0011 | 0.0013 | 0.0003 | 0.0004 | 0.0004 | 0.0008 | 0.0025 | 0.0019 | 0.0002 | 0.002 | 0.0009 | 0.0007 | 0.0012 |
Bud Periphery | 0.0005 | 0.0001 | 0.0005 | 0.002 | 0.0002 | 0.0003 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0005 | 0.0011 | 0.0016 | 0 | 0.0006 | 0.0003 | 0.0001 | 0.0003 |
Bud Site | 0.0009 | 0.001 | 0.0021 | 0.0029 | 0.0003 | 0.0003 | 0.0009 | 0.0102 | 0.0004 | 0.0007 | 0.0004 | 0.0022 | 0.0051 | 0.0006 | 0.0027 | 0.0012 | 0.0002 | 0.0002 |
Cell Periphery | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0011 | 0.0002 | 0.0005 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.8375 | 0.967 | 0.8864 | 0.779 | 0.8301 | 0.8996 | 0.8784 | 0.9652 | 0.9439 | 0.858 | 0.8804 | 0.9088 | 0.7167 | 0.9786 | 0.8922 | 0.8781 | 0.8584 | 0.8795 |
Cytoplasmic Foci | 0.0244 | 0.0069 | 0.0139 | 0.0199 | 0.0181 | 0.0145 | 0.0097 | 0.0038 | 0.012 | 0.0133 | 0.0065 | 0.0095 | 0.0311 | 0.0038 | 0.0155 | 0.0153 | 0.0161 | 0.0141 |
Eisosomes | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0225 | 0.0044 | 0.008 | 0.0151 | 0.015 | 0.016 | 0.021 | 0.0032 | 0.0057 | 0.0037 | 0.012 | 0.029 | 0.0397 | 0.0046 | 0.0084 | 0.0085 | 0.0151 | 0.0217 |
Endosome | 0.0457 | 0.0105 | 0.0362 | 0.0775 | 0.047 | 0.0331 | 0.0294 | 0.0048 | 0.0202 | 0.0453 | 0.0287 | 0.0226 | 0.0594 | 0.0046 | 0.0246 | 0.0253 | 0.0718 | 0.0396 |
Golgi | 0.0069 | 0.0016 | 0.0044 | 0.0047 | 0.0032 | 0.0064 | 0.0028 | 0.0004 | 0.0009 | 0.0008 | 0.0018 | 0.0023 | 0.0076 | 0.0008 | 0.0047 | 0.0032 | 0.004 | 0.0138 |
Lipid Particles | 0.0028 | 0 | 0.0018 | 0.0052 | 0.0029 | 0.002 | 0.0017 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0081 | 0.0001 | 0.0019 | 0.0043 | 0.0004 | 0.002 |
Mitochondria | 0.0077 | 0.0004 | 0.0103 | 0.0053 | 0.0438 | 0.0101 | 0.0022 | 0.0002 | 0.0004 | 0.0002 | 0.005 | 0.0029 | 0.0145 | 0.0002 | 0.0043 | 0.0008 | 0.0019 | 0.0109 |
None | 0.0088 | 0.0007 | 0.003 | 0.003 | 0.0016 | 0.0009 | 0.0065 | 0.0008 | 0.0011 | 0.0011 | 0.0014 | 0.0008 | 0.0026 | 0.0005 | 0.0008 | 0.0016 | 0.0007 | 0.0005 |
Nuclear Periphery | 0.0082 | 0.0006 | 0.0033 | 0.01 | 0.0026 | 0.0011 | 0.0081 | 0.0006 | 0.0011 | 0.0023 | 0.0315 | 0.0055 | 0.0154 | 0.0006 | 0.0015 | 0.0048 | 0.0053 | 0.0036 |
Nucleolus | 0.0005 | 0 | 0.0005 | 0.0005 | 0 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0066 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Nucleus | 0.0051 | 0.001 | 0.0025 | 0.0092 | 0.0018 | 0.0014 | 0.009 | 0.001 | 0.0012 | 0.0034 | 0.0095 | 0.0029 | 0.0199 | 0.0009 | 0.0011 | 0.0026 | 0.0031 | 0.0013 |
Peroxisomes | 0.0016 | 0.0001 | 0.0017 | 0.0049 | 0.0046 | 0.0016 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0076 | 0 | 0.0021 | 0.0025 | 0.0002 | 0.0012 |
Punctate Nuclear | 0.003 | 0.0001 | 0.0031 | 0.0019 | 0.0005 | 0.0002 | 0.0042 | 0.0001 | 0.0001 | 0.0003 | 0.0014 | 0.0002 | 0.0033 | 0.0001 | 0.0013 | 0.0102 | 0.0005 | 0.0002 |
Vacuole | 0.0085 | 0.0032 | 0.0078 | 0.0276 | 0.0116 | 0.0078 | 0.0131 | 0.004 | 0.0103 | 0.0638 | 0.0088 | 0.005 | 0.0185 | 0.0036 | 0.008 | 0.0291 | 0.0164 | 0.0044 |
Vacuole Periphery | 0.0017 | 0.0005 | 0.0016 | 0.0053 | 0.0023 | 0.0019 | 0.0023 | 0.0005 | 0.0013 | 0.0057 | 0.0058 | 0.0013 | 0.0046 | 0.0003 | 0.0014 | 0.0027 | 0.0042 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.1773 | 43.2357 | 51.4816 | 42.7446 | 43.0204 | 42.7178 | 33.8033 | 31.9066 | 34.7427 | 45.0882 |
Translational Efficiency | 1.6573 | 1.699 | 1.4016 | 1.4218 | 1.4144 | 1.528 | 2.0102 | 1.6952 | 1.5171 | 1.4382 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2165 | 672 | 1500 | 93 | 1911 | 1923 | 420 | 1299 | 4076 | 2595 | 1920 | 1392 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1085.36 | 1129.40 | 1223.04 | 1480.95 | 1045.90 | 1132.01 | 1367.64 | 1406.96 | 1066.86 | 1131.33 | 1254.67 | 1411.90 |
Standard Deviation | 151.56 | 214.30 | 208.25 | 208.24 | 152.20 | 174.96 | 204.45 | 235.17 | 153.13 | 185.95 | 215.87 | 234.20 |
Intensity Change Log 2 | — | 0.057383 | 0.172298 | 0.448349 | — | 0.114142 | 0.386944 | 0.427836 | — | 0.085516 | 0.281624 | 0.438319 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000138 | 0.000280 | 0.000085 | 0.001498 | 0.000183 | 0.000246 | 0.000428 | 0.001451 | 0.000159 | 0.000255 | 0.000160 | 0.001455 |
Bud Neck | 0.000779 | 0.000588 | 0.001999 | 0.003689 | 0.000663 | 0.000454 | 0.002636 | 0.001817 | 0.000724 | 0.000488 | 0.002138 | 0.001942 |
Bud Site | 0.015593 | 0.026814 | 0.007765 | 0.047817 | 0.016766 | 0.016033 | 0.016301 | 0.030275 | 0.016143 | 0.018825 | 0.009632 | 0.031447 |
Cell Periphery | 0.000125 | 0.000202 | 0.000200 | 0.000727 | 0.000150 | 0.000107 | 0.000203 | 0.000313 | 0.000137 | 0.000131 | 0.000200 | 0.000341 |
Cytoplasm | 0.694225* | 0.808516* | 0.656539* | 0.658472* | 0.732086* | 0.827475* | 0.540034* | 0.827676* | 0.711976* | 0.822566* | 0.631054* | 0.816371* |
Cytoplasmic Foci | 0.168183 | 0.100776 | 0.021569 | 0.098388 | 0.177420 | 0.105814 | 0.084708 | 0.037813 | 0.172514 | 0.104510 | 0.035381 | 0.041860 |
Eisosomes | 0.000010 | 0.000006 | 0.000007 | 0.000014 | 0.000009 | 0.000005 | 0.000025 | 0.000007 | 0.000010 | 0.000005 | 0.000011 | 0.000008 |
Endoplasmic Reticulum | 0.004110 | 0.002144 | 0.002270 | 0.001408 | 0.004172 | 0.001653 | 0.003451 | 0.000174 | 0.004139 | 0.001780 | 0.002528 | 0.000257 |
Endosome | 0.011627 | 0.007816 | 0.005878 | 0.019280 | 0.009101 | 0.005505 | 0.018673 | 0.006824 | 0.010443 | 0.006104 | 0.008677 | 0.007656 |
Golgi | 0.015232 | 0.009725 | 0.001516 | 0.009259 | 0.014060 | 0.012088 | 0.001448 | 0.008745 | 0.014682 | 0.011476 | 0.001501 | 0.008780 |
Lipid Particles | 0.000961 | 0.000978 | 0.001210 | 0.005727 | 0.001002 | 0.000815 | 0.003278 | 0.003254 | 0.000981 | 0.000857 | 0.001663 | 0.003419 |
Mitochondria | 0.001206 | 0.001381 | 0.000286 | 0.000269 | 0.000990 | 0.000585 | 0.000040 | 0.000899 | 0.001104 | 0.000791 | 0.000232 | 0.000856 |
Mitotic Spindle | 0.001198 | 0.000599 | 0.000165 | 0.002249 | 0.001746 | 0.002266 | 0.001172 | 0.008478 | 0.001455 | 0.001834 | 0.000385 | 0.008062 |
None | 0.000847 | 0.002208 | 0.001496 | 0.000034 | 0.000426 | 0.000441 | 0.000214 | 0.000123 | 0.000649 | 0.000899 | 0.001215 | 0.000117 |
Nuclear Periphery | 0.000033 | 0.000078 | 0.000182 | 0.000072 | 0.000021 | 0.000014 | 0.000405 | 0.000034 | 0.000028 | 0.000030 | 0.000231 | 0.000037 |
Nuclear Periphery Foci | 0.000208 | 0.001102 | 0.000393 | 0.004048 | 0.000264 | 0.000505 | 0.005102 | 0.001226 | 0.000235 | 0.000660 | 0.001423 | 0.001414 |
Nucleolus | 0.000067 | 0.000096 | 0.000268 | 0.000052 | 0.000050 | 0.000110 | 0.000294 | 0.000100 | 0.000059 | 0.000106 | 0.000274 | 0.000097 |
Nucleus | 0.003150 | 0.002887 | 0.026928 | 0.003898 | 0.002610 | 0.002672 | 0.027072 | 0.003171 | 0.002897 | 0.002728 | 0.026959 | 0.003220 |
Peroxisomes | 0.000419 | 0.000648 | 0.000371 | 0.001684 | 0.000346 | 0.000501 | 0.000813 | 0.001817 | 0.000385 | 0.000539 | 0.000468 | 0.001808 |
Vacuole | 0.061335 | 0.025807 | 0.267922* | 0.135075 | 0.024731 | 0.017559 | 0.291525* | 0.061833 | 0.044174 | 0.019695 | 0.273085* | 0.066727 |
Vacuole Periphery | 0.020553 | 0.007351 | 0.002951 | 0.006340 | 0.013201 | 0.005153 | 0.002177 | 0.003969 | 0.017106 | 0.005722 | 0.002782 | 0.004127 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.15 | 4.24 | -3.69 | -3.26 | -3.84 | -1.40 | -4.86 | -2.75 | -2.59 | -2.08 | -2.89 | -0.55 | -3.02 | -2.76 | -2.99 |
Bud Neck | 3.12 | -5.10 | -4.57 | -4.88 | -2.36 | 3.89 | -10.71 | -11.06 | -13.57 | 4.03 | 6.25 | -7.64 | -11.89 | -14.17 | 0.59 |
Bud Site | -5.20 | 8.71 | -3.10 | -1.89 | -3.92 | 0.56 | -0.44 | -5.14 | -5.42 | -4.32 | -2.53 | 7.84 | -6.31 | -4.83 | -9.20 |
Cell Periphery | -4.45 | -1.86 | -2.01 | -1.72 | -1.73 | 3.94 | -2.71 | -6.06 | -8.19 | -2.92 | 0.66 | -2.03 | -6.46 | -6.58 | -2.88 |
Cytoplasm | -11.09 | 3.58 | 1.30 | 5.27 | -0.10 | -12.67 | 11.97 | -8.84 | 2.33 | -16.48 | -18.72 | 9.08 | -11.37 | 3.12 | -16.39 |
Cytoplasmic Foci | 10.66 | 37.19 | 6.58 | 0.40 | -7.20 | 13.22 | 15.61 | 29.15 | 16.53 | 9.64 | 17.01 | 43.50 | 36.69 | 17.24 | -3.15 |
Eisosomes | 4.34 | 2.50 | -1.34 | -2.83 | -2.20 | 5.38 | -6.22 | 2.02 | -2.76 | 7.04 | 7.12 | -1.54 | 2.13 | -3.32 | 3.21 |
Endoplasmic Reticulum | 2.65 | 3.03 | 4.38 | 1.35 | 1.95 | 4.34 | 1.58 | 9.70 | 3.67 | 4.93 | 4.96 | 3.92 | 11.39 | 4.67 | 7.79 |
Endosome | 4.14 | 8.89 | -1.18 | -1.90 | -2.29 | 5.91 | -5.75 | 3.62 | -0.56 | 7.33 | 8.67 | 3.62 | 4.35 | -0.77 | 1.83 |
Golgi | 5.07 | 20.27 | 2.13 | 0.43 | -2.13 | 2.25 | 26.17 | 4.92 | 3.27 | -3.99 | 4.40 | 31.00 | 5.60 | 3.09 | -4.22 |
Lipid Particles | -0.15 | -1.92 | -4.96 | -4.87 | -4.65 | 1.06 | -7.79 | -6.61 | -6.58 | -0.73 | 0.88 | -5.64 | -7.41 | -7.33 | -5.35 |
Mitochondria | -0.21 | 4.49 | 3.48 | 1.37 | 0.25 | 2.12 | 6.00 | 1.10 | -0.50 | -3.16 | 1.21 | 6.58 | 1.84 | 0.40 | -2.23 |
Mitotic Spindle | 1.21 | 2.14 | -0.68 | -1.25 | -1.64 | -0.55 | 0.41 | -3.40 | -3.18 | -3.81 | -0.61 | 2.39 | -3.80 | -3.53 | -4.65 |
None | -1.15 | -0.86 | 1.74 | 2.00 | 2.62 | -0.20 | 2.76 | 4.91 | 6.41 | 2.22 | -0.66 | -1.10 | 2.14 | 2.76 | 2.53 |
Nuclear Periphery | -0.82 | -5.67 | -1.63 | 0.14 | 3.32 | 2.20 | -4.90 | -3.78 | -5.60 | 4.61 | -0.18 | -7.63 | -2.90 | -0.91 | 6.95 |
Nuclear Periphery Foci | -3.34 | -2.86 | -5.35 | -3.72 | -5.05 | -1.04 | -10.31 | -4.13 | -2.46 | 7.03 | -2.36 | -9.71 | -5.14 | -2.77 | -0.28 |
Nucleolus | -1.02 | -7.04 | 1.55 | 1.44 | 7.24 | -0.99 | -4.92 | -5.87 | 0.20 | 3.86 | -1.04 | -8.61 | -5.51 | 0.25 | 6.93 |
Nucleus | 0.69 | -12.62 | -1.83 | -1.99 | 11.72 | -0.43 | -7.01 | -6.30 | -5.09 | 6.56 | 0.86 | -14.51 | -5.40 | -5.26 | 13.73 |
Peroxisomes | -1.72 | 1.05 | -4.76 | -3.37 | -4.89 | -2.72 | -5.47 | -6.73 | -5.75 | -4.18 | -2.83 | -2.22 | -6.94 | -5.89 | -6.41 |
Vacuole | 9.48 | -22.88 | -4.73 | -6.76 | 6.52 | 4.38 | -20.18 | -15.81 | -18.12 | 15.21 | 13.29 | -30.48 | -11.43 | -18.68 | 22.77 |
Vacuole Periphery | 7.94 | 14.89 | 5.26 | 0.58 | -1.08 | 7.06 | 10.15 | 8.01 | 2.43 | -1.53 | 12.46 | 17.76 | 13.30 | 2.98 | -0.92 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|