Standard name
Human Ortholog
Description Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.84 0.87 0.92 0.84 0.93 0.86 0.84 0.89 0.75 0.82 0.71 0.7 0.93 0.95 0.97 0.74 0.64 0.68 0.69 0.77 0.84 0.69 0.79 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.07 0.16 0.18 0.34 0.31 0.18 0.44 0.3 0.51 0.48 0 0 0 0.09 0.05 0 0 0 0 0 0 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.07 0.07 0 0.06 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.15 0.05 0.16 0 0 0 0 0 0 0 0 0.07 0.07 0 0.18 0.32 0.29 0.15 0.06 0 0.15 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 5 3 2 3 8
Bud 0 1 0 1 9 15 13 6 25 19 37 38 0 0 0 0 0 0 2 5 0 4 3 13
Bud Neck 0 0 0 0 2 3 4 3 1 3 4 7 1 1 2 0 0 0 1 3 0 1 1 2
Bud Site 0 0 0 0 0 2 2 0 5 3 6 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 2 3 2 3 6 3 7 6 6 0 1 1 0 1 0 0 0 0 0 1 1
Cytoplasm 131 384 202 294 742 784 1010 833 637 815 668 860 225 595 630 87 75 62 140 378 201 250 349 378
Endoplasmic Reticulum 0 2 2 0 0 1 1 0 0 0 0 3 3 3 0 4 5 0 0 1 2 2 2 8
Endosome 3 5 0 6 0 1 2 4 1 0 2 1 1 0 3 3 4 2 2 9 2 1 4 14
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 2 1 0 2
Mitochondria 8 2 16 55 141 307 371 166 369 294 473 590 1 0 0 11 6 0 6 6 3 13 15 41
Nucleus 3 7 2 3 1 5 4 9 5 2 4 6 8 5 5 17 8 6 8 31 6 16 14 1
Nuclear Periphery 2 1 1 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 2 1 1 2 2 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 2 0 0 1 1 1 1 0 0 0 0 0 0 0 0 1 0 3
SpindlePole 0 0 0 1 3 4 10 20 11 23 21 28 0 0 0 1 1 0 2 7 4 3 5 21
Vac/Vac Membrane 18 68 11 55 12 3 2 3 13 5 5 2 18 47 22 21 37 26 30 29 7 53 37 22
Unique Cell Count 156 443 220 351 799 913 1201 933 848 994 935 1221 243 629 650 117 117 91 202 489 241 363 445 527
Labelled Cell Count 165 472 234 417 915 1127 1425 1052 1071 1173 1231 1551 259 652 664 145 137 96 202 489 241 363 445 527


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.7 4.2 4.1 3.4 3.1 2.8 3.2 2.8 2.7 2.6 2.6 5.9 5.6 5.4 5.8 6.2 7.0 6.4 6.1 5.8
Std Deviation (1e-4) 0.9 1.0 0.8 1.2 1.3 1.5 1.3 1.4 1.2 0.8 1.1 0.6 1.1 1.0 1.0 1.0 1.1 1.9 1.2 1.4 1.3
Intensity Change (Log2) – – – -0.02 -0.31 -0.42 -0.6 -0.38 -0.58 -0.63 -0.69 -0.71 0.49 0.42 0.37 0.48 0.56 0.73 0.62 0.55 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.8 0.5 -2.4 -3.0 -1.1 -5.4 -3.6 -6.3 -6.6 0.3 1.5 3.2 -4.4 -6.3 -5.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -3.9 0 0 0.7 -0.8 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 5.2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.9 0 0 0 0 0 0 0 0 1.1 1.3 -1.1 3.9 6.7 5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2726 1.6803 1.3504 1.0846 1.4316 1.445 1.6188 2.1293 2.0922 1.345 1.853 1.8934 -0.8814 0.3071 -0.1855 0.2681 -0.1915 0.1784
Actin 0.0346 0.0048 0.005 0.0075 0.0047 0.0063 0.0388 0.0008 0.0159 0.0017 0.0184 0.0016 0.0125 0.0003 0.0074 0.0198 0.0414 0.0024
Bud 0.0055 0.0154 0.017 0.0094 0.0196 0.0054 0.0014 0.0007 0.0004 0.001 0.0005 0.0004 0.0002 0.0002 0.0004 0.0004 0.0006 0.0002
Bud Neck 0.0058 0.0008 0.0024 0.0009 0.0073 0.0045 0.0026 0.0005 0.0011 0.0006 0.0012 0.0014 0.0032 0.0087 0.0006 0.0011 0.0021 0.0027
Bud Periphery 0.0044 0.0041 0.0038 0.0129 0.0046 0.0041 0.002 0.0013 0.0003 0.0024 0.0006 0.0004 0.0002 0.0004 0.0006 0.0003 0.0005 0.0001
Bud Site 0.0109 0.0279 0.019 0.008 0.0351 0.0031 0.0127 0.0056 0.0091 0.0007 0.0082 0.0004 0.0066 0.0024 0.0014 0.0013 0.0023 0.0002
Cell Periphery 0.0007 0.0005 0.0008 0.0001 0.0006 0.0003 0.0004 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0 0.0001 0
Cytoplasm 0.2426 0.4242 0.4183 0.4515 0.2754 0.5113 0.4169 0.6149 0.5822 0.5421 0.4411 0.5977 0.5127 0.6183 0.5853 0.4753 0.5576 0.7186
Cytoplasmic Foci 0.0645 0.0253 0.0281 0.0562 0.039 0.0514 0.0258 0.0081 0.0162 0.0235 0.0268 0.014 0.0192 0.0096 0.01 0.0257 0.0209 0.0064
Eisosomes 0.001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0004 0
Endoplasmic Reticulum 0.0059 0.0031 0.0029 0.0015 0.004 0.0043 0.0074 0.003 0.0053 0.0056 0.0033 0.0044 0.0064 0.0037 0.0047 0.0072 0.0038 0.004
Endosome 0.0508 0.0075 0.0203 0.0099 0.0225 0.0157 0.0244 0.0048 0.012 0.0114 0.0236 0.01 0.0124 0.0026 0.0145 0.0154 0.0135 0.0102
Golgi 0.0177 0.0024 0.01 0.0096 0.0071 0.0061 0.0069 0.001 0.004 0.0039 0.0083 0.0024 0.0033 0.0002 0.0033 0.0045 0.0062 0.0007
Lipid Particles 0.0297 0.0069 0.0104 0.0067 0.0896 0.0078 0.01 0.0013 0.0018 0.0125 0.0102 0.0021 0.0094 0.0005 0.007 0.0064 0.0227 0.0119
Mitochondria 0.0192 0.0065 0.0179 0.0176 0.0104 0.0228 0.007 0.0014 0.0054 0.0072 0.0043 0.0079 0.0011 0.0002 0.0023 0.002 0.0029 0.0008
None 0.3954 0.3463 0.3405 0.2218 0.3283 0.2357 0.3352 0.288 0.2747 0.2861 0.3969 0.2819 0.3812 0.2995 0.296 0.2478 0.1912 0.0962
Nuclear Periphery 0.0159 0.005 0.0068 0.004 0.0051 0.0105 0.0116 0.0022 0.0057 0.0082 0.0045 0.0042 0.0044 0.0033 0.0053 0.0158 0.0062 0.0078
Nucleolus 0.0026 0.0027 0.0035 0.0039 0.002 0.0035 0.0053 0.0023 0.0005 0.0005 0.0005 0.0003 0.0006 0.0003 0.0003 0.0006 0.0009 0.0006
Nucleus 0.0225 0.0743 0.0524 0.0679 0.0186 0.0533 0.0504 0.0419 0.0541 0.0716 0.013 0.0525 0.0119 0.0435 0.0467 0.085 0.1034 0.1247
Peroxisomes 0.0366 0.0066 0.0126 0.0583 0.0367 0.009 0.0224 0.0005 0.0027 0.0109 0.0254 0.0096 0.007 0.0006 0.0073 0.0733 0.0086 0.0008
Punctate Nuclear 0.0216 0.0333 0.024 0.0499 0.0872 0.0414 0.0092 0.0166 0.0055 0.0068 0.008 0.0068 0.0047 0.0037 0.0035 0.0156 0.0127 0.0092
Vacuole 0.0096 0.0021 0.003 0.0016 0.0016 0.0023 0.0074 0.0041 0.0022 0.0022 0.0043 0.0016 0.0022 0.0016 0.0024 0.002 0.0014 0.002
Vacuole Periphery 0.0026 0.0003 0.0011 0.0006 0.0005 0.0012 0.002 0.0005 0.0006 0.0009 0.0005 0.0003 0.0003 0.0001 0.0009 0.0006 0.0005 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.6794 28.2914 24.9652 23.5533 21.071 21.372 33.6307 29.4819 29.1444 24.1016
Translational Efficiency 2.1917 2.0781 1.5707 1.5465 1.7228 1.6556 1.6775 1.6352 1.305 1.5344

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1751 1549 319 1150 1799 1252 1970 1520 3550 2801 2289 2670

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 746.14 773.48 988.97 1050.25 711.42 756.26 949.85 1081.22 728.55 765.78 955.30 1067.88
Standard Deviation 87.64 111.18 124.35 149.96 90.56 708.79 141.67 154.06 90.81 481.11 140.04 153.08
Intensity Change Log 2 — 0.051918 0.406480 0.493215 — 0.088181 0.416998 0.603887 — 0.069731 0.411624 0.548293

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000393 0.000296 0.001438 0.003007 0.000092 0.000415 0.000263 0.001459 0.000240 0.000349 0.000427 0.002126
Bud Neck 0.008881 0.010047 0.005556 0.018741 0.004846 0.018595 0.009183 0.018369 0.006837 0.013868 0.008678 0.018529
Bud Site 0.008589 0.005843 0.010168 0.090668 0.002668 0.009183 0.005034 0.053827 0.005589 0.007336 0.005750 0.069695
Cell Periphery 0.000226 0.000140 0.000323 0.000250 0.000120 0.000212 0.000105 0.000197 0.000172 0.000172 0.000135 0.000220
Cytoplasm 0.494357* 0.468109* 0.202189* 0.110059* 0.500258* 0.418106* 0.287084* 0.189234* 0.497347* 0.445759* 0.275253* 0.155132*
Cytoplasmic Foci 0.093117 0.092804 0.008317 0.042404 0.110893 0.163754 0.001795 0.032226 0.102125 0.124518 0.002704 0.036610
Eisosomes 0.000063 0.000060 0.000078 0.000034 0.000059 0.000113 0.000011 0.000029 0.000061 0.000084 0.000020 0.000031
Endoplasmic Reticulum 0.001049 0.001988 0.013104 0.003606 0.000930 0.001233 0.002426 0.003594 0.000989 0.001651 0.003914 0.003600
Endosome 0.003500 0.005941 0.004442 0.020590 0.004152 0.011417 0.000810 0.015625 0.003830 0.008389 0.001316 0.017763
Golgi 0.000888 0.001402 0.000203 0.011237 0.000864 0.003225 0.000079 0.006843 0.000876 0.002217 0.000097 0.008736
Lipid Particles 0.004204 0.003794 0.002828 0.001925 0.004471 0.010030 0.000289 0.001604 0.004339 0.006582 0.000643 0.001742
Mitochondria 0.003889 0.002977 0.003734 0.010176 0.003895 0.010819 0.001056 0.006542 0.003892 0.006482 0.001429 0.008107
Mitotic Spindle 0.002378 0.003514 0.003599 0.427734* 0.002166 0.003972 0.021427 0.354404* 0.002270 0.003719 0.018943 0.385988*
None 0.004571 0.007260 0.003462 0.005076 0.007201 0.006351 0.009167 0.014208 0.005904 0.006854 0.008372 0.010275
Nuclear Periphery 0.001111 0.000870 0.002298 0.002298 0.000635 0.000923 0.001414 0.001557 0.000870 0.000894 0.001537 0.001876
Nuclear Periphery Foci 0.000369 0.001208 0.005873 0.006301 0.000647 0.000734 0.000658 0.006626 0.000510 0.000996 0.001385 0.006486
Nucleolus 0.001171 0.002999 0.000694 0.004668 0.001381 0.003092 0.000520 0.005203 0.001277 0.003041 0.000544 0.004973
Nucleus 0.341099* 0.346447* 0.638546* 0.158685* 0.331087* 0.281612* 0.642971* 0.232582* 0.336025* 0.317467* 0.642354* 0.200754*
Peroxisomes 0.001484 0.004217 0.000652 0.011019 0.002758 0.011566 0.000365 0.011003 0.002130 0.007502 0.000405 0.011010
Vacuole 0.028124 0.039218 0.092221 0.054909 0.020402 0.043196 0.015244 0.039206 0.024211 0.040996 0.025972 0.045969
Vacuole Periphery 0.000537 0.000865 0.000277 0.016612 0.000474 0.001453 0.000099 0.005660 0.000505 0.001128 0.000124 0.010377

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.34 -4.92 -3.85 -6.05 0.91 -3.11 -9.71 -7.89 -4.28 -5.06 -0.94 -3.30 -6.64 -7.63 -5.59
Bud Neck -0.96 2.12 -12.15 -10.51 -13.31 -6.99 -7.04 -15.00 -4.64 -8.21 -6.15 -5.33 -18.98 -10.65 -11.63
Bud Site 1.68 -0.30 -11.94 -13.35 -10.62 -4.92 -4.68 -16.05 -12.71 -14.18 -1.78 -1.65 -19.20 -18.79 -18.60
Cell Periphery 2.87 -2.93 1.15 -3.98 4.95 -7.13 0.10 -5.88 1.90 -5.64 0.13 1.24 -0.77 -1.57 -3.33
Cytoplasm 3.82 27.34 53.41 43.34 6.93 8.48 32.83 43.90 28.36 13.11 8.47 42.94 66.89 49.36 18.45
Cytoplasmic Foci -0.12 24.67 18.19 16.86 -5.68 -8.26 30.06 21.89 25.52 -11.37 -5.62 39.97 28.39 29.64 -13.51
Eisosomes 0.13 -3.87 2.29 3.10 6.05 -10.36 16.80 2.66 12.13 -15.28 -6.58 10.90 3.31 11.12 -10.97
Endoplasmic Reticulum -6.65 -12.53 -11.84 -8.31 8.98 -2.02 -10.15 -12.95 -12.46 -3.80 -6.28 -15.49 -17.66 -14.18 1.24
Endosome -4.24 -2.54 -5.12 -1.89 -3.41 -6.39 7.46 -3.87 3.28 -8.66 -7.54 7.71 -6.36 1.22 -10.78
Golgi -1.12 1.84 -2.85 -2.36 -4.34 -3.39 3.60 -2.26 1.30 -3.92 -3.42 3.56 -3.57 -0.59 -5.91
Lipid Particles 0.79 2.15 4.98 5.18 3.40 -4.77 11.40 7.36 7.33 -11.05 -3.71 12.18 8.54 8.68 -9.90
Mitochondria 1.16 0.98 0.24 -1.22 -0.90 -3.36 2.75 1.16 4.35 -2.27 -2.39 3.82 1.13 3.63 -3.63
Mitotic Spindle -1.06 -1.24 -31.61* -31.49* -30.39* -1.31 -9.72 -32.33* -32.12* -27.26* -1.67 -9.83 -45.09 -44.86* -38.22*
None -3.03 2.33 -5.29 -2.22 -8.13 0.91 -2.86 -4.93 -5.35 -3.46 -1.16 -4.86 -7.20 -5.83 -3.92
Nuclear Periphery 0.88 -6.77 -10.88 -16.27 -4.87 -2.43 -19.44 -20.72 -14.71 -7.86 -0.43 -12.22 -19.48 -22.29 -10.45
Nuclear Periphery Foci -5.58 -6.01 -9.53 -8.78 -3.81 0.35 -2.65 -8.48 -8.65 -7.14 -2.63 -4.73 -12.30 -11.95 -9.15
Nucleolus -3.06 2.85 -9.99 -8.46 -10.28 -3.18 -2.64 -11.32 -9.28 -9.44 -4.27 -2.93 -15.04 -12.45 -12.70
Nucleus -0.84 -17.77 7.02 7.25 20.52 4.98 -36.71 4.50 -0.12 35.69 2.47 -43.74 8.05 5.48 44.09
Peroxisomes -6.31 5.24 -5.73 -1.50 -7.13 -6.41 4.14 -2.50 4.52 -6.62 -7.98 5.35 -5.34 2.83 -9.48
Vacuole -6.24 -10.88 -11.77 -7.78 4.49 -9.37 -4.31 -14.73 -7.16 -11.97 -11.06 -8.60 -18.61 -10.62 -10.42
Vacuole Periphery -1.44 2.61 -2.71 -2.36 -2.98 -3.99 6.41 -1.93 0.38 -2.90 -3.79 6.88 -3.29 -1.97 -4.10
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (51%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ast1

Ast1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ast1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available