Standard name
Human Ortholog
Description Component of the U5 snRNP complex; required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.94 0.91 0.85 0.89 0.87 0.76 0.77 0.71 0.63 0.64 0.67 0.89 0.96 0.89 0.82 0.76 0.86 0.72 0.73 0.67 0.77 0.75 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.05 0 0.14 0.3 0.25 0.38 0.4 0.45 0.31 0 0 0 0 0 0 0.07 0.06 0.1 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0 0.08 0.07 0.06 0.06 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.07 0.11 0.11 0.08 0.06 0.07 0.08 0 0.08 0 0.07 0.1 0.09 0.13 0.23 0.31 0.19 0 0.05 0.06 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 2 3 0 1 1
Bud 1 1 0 0 1 1 5 4 2 9 5 6 0 0 0 0 0 1 3 5 1 1 6 9
Bud Neck 0 0 12 3 8 3 5 6 3 5 9 5 2 1 3 2 2 3 0 3 1 0 2 5
Bud Site 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 2 1 0 1 2 1 1 0 8 5 12 0 1 3 0 0 2
Cytoplasm 154 303 238 117 218 236 261 197 157 151 175 190 163 175 211 393 325 387 135 244 174 95 180 163
Endoplasmic Reticulum 1 0 0 0 3 0 1 0 0 0 0 0 0 1 0 33 24 28 2 2 2 2 1 1
Endosome 0 0 1 1 0 1 0 1 0 0 0 0 1 0 1 1 1 0 0 5 2 0 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 1 0
Mitochondria 17 5 0 7 6 38 104 65 84 96 122 89 0 0 0 4 4 10 12 20 24 6 8 5
Nucleus 2 3 11 5 3 1 15 7 5 11 6 17 10 2 3 14 11 20 15 23 16 7 12 9
Nuclear Periphery 0 0 1 0 0 2 0 0 1 2 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 2 8 2 3 2 6 0 0 0 0 1 1 1 4 4 1 7 4
Vac/Vac Membrane 16 24 30 15 19 17 24 20 5 20 6 21 19 17 32 111 135 85 6 16 16 4 11 9
Unique Cell Count 173 324 262 138 245 272 344 255 220 239 273 284 183 182 238 479 429 452 188 335 259 125 240 220
Labelled Cell Count 191 337 295 149 258 300 418 310 262 297 327 336 196 197 251 568 509 548 188 335 259 125 240 220


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.9 5.5 4.7 5.1 4.2 4.0 4.3 3.2 3.9 3.4 3.9 5.7 6.3 6.2 8.0 7.7 7.2 5.9 6.2 6.5
Std Deviation (1e-4) 1.5 1.9 1.6 1.4 2.0 1.7 1.3 1.5 1.0 1.2 1.0 1.0 1.6 1.9 1.9 3.1 3.2 3.3 2.2 2.2 2.2
Intensity Change (Log2) – – – -0.23 -0.12 -0.39 -0.45 -0.34 -0.77 -0.48 -0.69 -0.5 0.05 0.19 0.16 0.54 0.49 0.39 0.1 0.17 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.9 6.0 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8611 2.4758 2.4791 2.2258 1.8906 2.5298 1.6101 2.6137 2.0803 1.8372 1.8102 2.0871 4.8786 3.1236 1.9034 2.0311 2.0784 2.268
Actin 0.0127 0.0015 0.0015 0.0004 0.0037 0.0039 0.0009 0.0005 0.001 0.0003 0.0211 0.0015 0.0247 0.0025 0.0029 0.0539 0.0081 0.003
Bud 0.0014 0.0028 0.0006 0.0003 0.0006 0.0005 0.0002 0.0004 0.0003 0.0001 0.0001 0.0008 0.0013 0.0004 0.0005 0.0023 0.0501 0.0018
Bud Neck 0.0028 0.0013 0.0019 0.0012 0.0037 0.0046 0.0016 0.0015 0.001 0.0009 0.001 0.0042 0.0042 0.0006 0.0032 0.0029 0.0014 0.003
Bud Periphery 0.0024 0.003 0.001 0.0002 0.0006 0.0007 0.0001 0.0002 0.0002 0 0.0001 0.0003 0.0027 0.0006 0.0005 0.0022 0.022 0.0016
Bud Site 0.0053 0.01 0.0013 0.0004 0.0077 0.0004 0.0013 0.0038 0.0014 0.0002 0.0002 0.0003 0.0098 0.0042 0.0049 0.0038 0.0056 0.0003
Cell Periphery 0.0003 0.0002 0.0001 0 0.0002 0 0 0 0 0 0 0 0.0087 0.0076 0.0002 0.0001 0.0014 0.0017
Cytoplasm 0.2883 0.4113 0.3358 0.3346 0.2762 0.319 0.3098 0.393 0.3618 0.4802 0.3591 0.2671 0.4043 0.6412 0.5387 0.4745 0.4982 0.4405
Cytoplasmic Foci 0.0128 0.0071 0.0115 0.0083 0.0251 0.0097 0.0062 0.0027 0.0062 0.0041 0.0068 0.0048 0.011 0.0032 0.006 0.0218 0.0236 0.0052
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0 0 0 0 0.0009 0 0.0003 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0045 0.0036 0.0024 0.0021 0.0038 0.0022 0.0035 0.0033 0.0022 0.003 0.0016 0.0019 0.0141 0.0034 0.0037 0.0062 0.007 0.003
Endosome 0.012 0.0044 0.0058 0.0042 0.0243 0.0055 0.0048 0.0034 0.0026 0.0044 0.0052 0.0038 0.0286 0.002 0.0089 0.0446 0.0574 0.0054
Golgi 0.0026 0.0003 0.001 0.0001 0.0034 0.0006 0.0002 0.0001 0.0001 0.0001 0.0013 0.0003 0.0094 0.0003 0.0066 0.0555 0.0105 0.0035
Lipid Particles 0.0054 0.0002 0.0031 0.0002 0.0024 0.0009 0.0006 0.0002 0.0003 0 0.0037 0.0017 0.0111 0.0002 0.0013 0.0061 0.0282 0.0003
Mitochondria 0.0042 0.001 0.0048 0.001 0.0024 0.0021 0.0006 0.0005 0.0006 0.0006 0.0041 0.0005 0.0113 0.0003 0.01 0.0099 0.0135 0.0019
None 0.3676 0.2646 0.2972 0.1771 0.3756 0.2297 0.07 0.0525 0.06 0.0174 0.1385 0.0525 0.0622 0.0468 0.0696 0.0034 0.0067 0.0501
Nuclear Periphery 0.0164 0.0098 0.0082 0.0092 0.0139 0.0099 0.012 0.0144 0.0114 0.0134 0.0088 0.0107 0.0237 0.0097 0.0128 0.0099 0.0196 0.0129
Nucleolus 0.0016 0.0018 0.0029 0.0018 0.0013 0.0021 0.0016 0.0019 0.0017 0.0006 0.0011 0.0019 0.0009 0.001 0.0006 0.0006 0.0008 0.001
Nucleus 0.2325 0.2565 0.2946 0.4034 0.2171 0.3672 0.5654 0.4914 0.5165 0.4582 0.4114 0.5927 0.3399 0.2603 0.3178 0.2029 0.1979 0.4304
Peroxisomes 0.009 0.0015 0.0025 0.0004 0.002 0.0033 0.0003 0.0002 0.0008 0.0001 0.0085 0.0033 0.008 0.0004 0.001 0.0203 0.0173 0.0001
Punctate Nuclear 0.0136 0.0161 0.0209 0.0534 0.0324 0.0358 0.0186 0.0281 0.0306 0.015 0.0244 0.0504 0.0065 0.0077 0.0035 0.0738 0.0072 0.0256
Vacuole 0.0035 0.0027 0.0021 0.0012 0.0029 0.0014 0.002 0.0018 0.0011 0.0011 0.0014 0.001 0.0152 0.0072 0.0048 0.0035 0.0158 0.0071
Vacuole Periphery 0.001 0.0004 0.0004 0.0003 0.0007 0.0004 0.0004 0.0003 0.0002 0.0003 0.0004 0.0002 0.0019 0.0003 0.0023 0.0017 0.0076 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1989 7.3886 8.224 10.9063 13.3952 13.4235 9.8052 13.5508 11.0213 11.9742
Translational Efficiency 0.9603 0.8087 0.8631 0.6644 0.3919 0.486 0.7087 0.5103 0.6473 0.5832

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the U5 snRNP complex; required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aar2

Aar2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aar2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available