Standard name | |
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Human Ortholog | |
Description | Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 4 | 8 | 2 | 4 | 2 |
Bud | 3 | 3 | 6 | 4 | 5 | 11 | 11 | 20 | 14 | 9 | 4 | 3 | 0 | 0 | 0 | 18 | 6 | 10 | 2 | 2 | 2 | 0 | 10 | 8 |
Bud Neck | 2 | 1 | 8 | 1 | 10 | 6 | 5 | 7 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 2 | 1 | 0 | 2 | 5 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 8 | 4 | 2 | 1 | 2 | 0 | 0 | 0 | 4 | 10 | 5 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 6 | 1 | 4 | 0 | 1 | 0 | 26 | 12 | 22 | 0 | 2 | 4 | 0 | 4 | 2 |
Cytoplasm | 152 | 277 | 226 | 172 | 207 | 159 | 237 | 123 | 86 | 107 | 49 | 39 | 266 | 362 | 421 | 152 | 63 | 35 | 119 | 212 | 122 | 135 | 227 | 199 |
Endoplasmic Reticulum | 0 | 2 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 1 | 2 | 1 | 33 | 22 | 14 | 6 | 0 | 1 | 1 | 0 | 1 |
Endosome | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 133 | 91 | 62 | 7 | 5 | 4 | 2 | 1 | 3 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 14 | 9 | 14 | 1 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 26 | 12 | 15 | 24 | 51 | 112 | 152 | 154 | 134 | 153 | 112 | 102 | 0 | 4 | 2 | 100 | 70 | 41 | 41 | 58 | 59 | 22 | 34 | 25 |
Nucleus | 0 | 1 | 0 | 3 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 1 | 15 | 17 | 6 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 102 | 59 | 62 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 10 | 7 | 18 | 24 | 10 | 8 | 9 | 4 | 0 | 1 | 1 | 150 | 119 | 79 | 6 | 5 | 4 | 3 | 12 | 1 |
Vac/Vac Membrane | 0 | 4 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 2 | 0 | 6 | 1 | 0 | 2 | 76 | 59 | 58 | 13 | 10 | 5 | 2 | 4 | 13 |
Unique Cell Count | 167 | 295 | 240 | 183 | 258 | 252 | 359 | 268 | 204 | 248 | 154 | 137 | 268 | 370 | 429 | 622 | 407 | 307 | 212 | 315 | 226 | 178 | 313 | 271 |
Labelled Cell Count | 188 | 306 | 261 | 206 | 290 | 301 | 432 | 346 | 256 | 295 | 186 | 163 | 270 | 375 | 432 | 831 | 540 | 412 | 212 | 315 | 226 | 178 | 313 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 3.6 | 2.8 | 2.7 | 3.1 | 2.6 | 2.4 | 2.8 | 2.8 | 2.5 | 2.6 | 2.9 | 4.0 | 3.8 | 3.8 | 5.3 | 6.2 | 6.2 | 3.5 | 3.8 | 4.2 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.5 | 1.0 | 1.2 | 0.7 | 1.1 | 1.1 | 1.2 | 0.9 | 0.9 | 1.4 | 1.6 | 1.3 | 1.2 | 1.4 | 1.4 | 1.5 | 1.0 | 1.4 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.15 | -0.15 | -0.25 | -0.02 | -0.03 | -0.19 | -0.13 | 0.04 | 0.5 | 0.42 | 0.43 | 0.89 | 1.13 | 1.13 | 0.31 | 0.41 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.7 | 6.2 | 7.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.1495 | 0.0066 | 0.0012 | 0.0026 | 0.0006 | 0.0435 | 0.0427 | 0.0142 | 0.011 | 0.0094 | 0.0076 | 0.0061 | 0 | 0.0057 | 0.0002 | 0.0094 | 0.0005 | 0.0002 |
Bud | 0.0012 | 0.0004 | 0.0004 | 0.0159 | 0.0071 | 0.0006 | 0.0005 | 0.0005 | 0.0011 | 0.0143 | 0.0005 | 0.0019 | 0 | 0.0007 | 0 | 0.0009 | 0.0001 | 0 |
Bud Neck | 0.008 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0027 | 0.0006 | 0.0005 | 0.0038 | 0.0022 | 0.0002 | 0.0005 | 0 | 0.0004 | 0.0002 | 0.0009 | 0 | 0.0001 |
Bud Periphery | 0.0038 | 0.0008 | 0.0035 | 0.0559 | 0.0252 | 0.0024 | 0.0015 | 0.0006 | 0.0017 | 0.0054 | 0.0026 | 0.0023 | 0 | 0.0013 | 0 | 0.0022 | 0.0002 | 0 |
Bud Site | 0.0133 | 0.0006 | 0.0007 | 0.0078 | 0.0003 | 0.0003 | 0.0018 | 0.0106 | 0.0092 | 0.0332 | 0.0004 | 0.0013 | 0 | 0.0125 | 0.0003 | 0.0007 | 0 | 0 |
Cell Periphery | 0.014 | 0.0014 | 0.0025 | 0.0021 | 0.0006 | 0.0005 | 0.0006 | 0.0006 | 0.0007 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0013 | 0.0002 | 0.0008 | 0.0001 | 0.0001 |
Cytoplasm | 0.1795 | 0.1943 | 0.1561 | 0.3252 | 0.1168 | 0.1363 | 0.0209 | 0.0631 | 0.0602 | 0.0498 | 0.0408 | 0.0769 | 0.0121 | 0.1361 | 0.0424 | 0.0536 | 0.042 | 0.0786 |
Cytoplasmic Foci | 0.0836 | 0.0115 | 0.007 | 0.0179 | 0.0182 | 0.0439 | 0.0687 | 0.0192 | 0.0427 | 0.0407 | 0.06 | 0.0368 | 0.0008 | 0.0111 | 0.0077 | 0.0182 | 0.0061 | 0.0324 |
Eisosomes | 0.0119 | 0.0012 | 0.0014 | 0.0002 | 0.0005 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0773 | 0.0033 | 0.0026 | 0.0056 | 0.0102 | 0.0117 | 0.0018 | 0.0017 | 0.0015 | 0.0023 | 0.0011 | 0.001 | 0.0001 | 0.0012 | 0.0012 | 0.0012 | 0.0014 | 0.0018 |
Endosome | 0.0498 | 0.007 | 0.0019 | 0.0182 | 0.015 | 0.0155 | 0.0484 | 0.0044 | 0.0362 | 0.0126 | 0.0124 | 0.0097 | 0 | 0.0034 | 0.0024 | 0.0387 | 0.0193 | 0.0154 |
Golgi | 0.0346 | 0.0075 | 0.0006 | 0.0051 | 0.0038 | 0.0051 | 0.0153 | 0.005 | 0.0226 | 0.0073 | 0.0039 | 0.0045 | 0 | 0.0079 | 0.0004 | 0.0353 | 0.0073 | 0.0008 |
Lipid Particles | 0.0665 | 0.0016 | 0.001 | 0.0005 | 0.0028 | 0.0041 | 0.0276 | 0.0019 | 0.0065 | 0.0152 | 0.018 | 0.0036 | 0 | 0.0003 | 0.0011 | 0.014 | 0.0035 | 0.008 |
Mitochondria | 0.0057 | 0.0037 | 0.002 | 0.123 | 0.0917 | 0.0536 | 0.0119 | 0.003 | 0.0092 | 0.0068 | 0.0028 | 0.002 | 0 | 0.003 | 0.0002 | 0.0835 | 0.1571 | 0.0004 |
None | 0.2698 | 0.7574 | 0.8157 | 0.4013 | 0.6951 | 0.648 | 0.6696 | 0.8645 | 0.7756 | 0.7596 | 0.7832 | 0.8423 | 0.9865 | 0.8132 | 0.9416 | 0.7196 | 0.7575 | 0.8589 |
Nuclear Periphery | 0.0064 | 0.0003 | 0.0003 | 0.0018 | 0.0006 | 0.0012 | 0.005 | 0.0004 | 0.0006 | 0.0027 | 0.0007 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0004 |
Nucleolus | 0.0017 | 0.0002 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0013 | 0.0005 | 0.0004 | 0.004 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 |
Nucleus | 0.0026 | 0.0003 | 0.0005 | 0.0043 | 0.0006 | 0.0005 | 0.0019 | 0.0005 | 0.0007 | 0.0063 | 0.0003 | 0.0008 | 0 | 0.0002 | 0.0002 | 0.0009 | 0.0001 | 0.0001 |
Peroxisomes | 0.0067 | 0.0005 | 0.0005 | 0.0013 | 0.0014 | 0.0239 | 0.0432 | 0.0047 | 0.0105 | 0.0164 | 0.0616 | 0.0075 | 0 | 0.0003 | 0.0001 | 0.0045 | 0.0007 | 0.0007 |
Punctate Nuclear | 0.0062 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0027 | 0.0192 | 0.0018 | 0.001 | 0.0071 | 0.0019 | 0.0014 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.001 |
Vacuole | 0.0065 | 0.0008 | 0.0009 | 0.0067 | 0.005 | 0.0014 | 0.0124 | 0.0015 | 0.0036 | 0.0026 | 0.0006 | 0.0006 | 0 | 0.0004 | 0.0006 | 0.011 | 0.0013 | 0.0006 |
Vacuole Periphery | 0.0014 | 0.0002 | 0.0001 | 0.0033 | 0.0039 | 0.0012 | 0.0045 | 0.0002 | 0.0008 | 0.0009 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0032 | 0.002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.7502 | 6.0663 | 10.8034 | 7.9561 | 9.2383 | 8.1387 | 10.7338 | 12.0053 | 10.8046 | 10.9316 |
Translational Efficiency | 1.0373 | 0.8096 | 0.3896 | 0.5288 | 0.3655 | 0.7603 | 0.6316 | 0.5896 | 0.5608 | 0.4814 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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