Standard name
Human Ortholog
Description Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.7 0.74 0.88 0.91 0.87 0.86 0.93 0.9 0.91 0.93 0.92 0.88 0.91 0.97 0.59 0.54 0.58 0.07 0.11 0.09 0.14 0.17 0.15
Endoplasmic Reticulum 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0.12 0.07 0 0 0 0 0.13 0.09 0.09 0.16 0.13 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.17 0.08 0 0 0.06 0.07 0 0.1 0.11 0.14 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.17 0.17 0.1 0.1 0.07 0.12 0.07 0.05 0.06 0 0 0 0 0 0.47 0.49 0.46 0.76 0.68 0.73 0.66 0.64 0.64
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 2 0 0 1 1 0 0 0 0 0 0 1 0 0 4
Bud 0 3 0 0 2 2 6 10 10 12 8 5 2 3 2 0 0 0 0 2 1 1 3 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 3 4 2 2 0 1 1 4 6 0 1 0 0 0 0 0 0 0
Cytoplasm 137 89 90 145 251 246 380 534 341 409 252 370 169 298 422 178 125 76 18 18 12 22 38 40
Endoplasmic Reticulum 4 9 7 3 6 10 5 3 10 5 0 4 23 24 10 6 10 2 35 14 12 26 27 18
Endosome 7 0 0 0 0 1 0 4 0 0 0 0 0 0 2 8 7 0 2 5 2 1 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 2 0 0 0 2
Mitochondria 30 22 10 5 4 18 33 18 39 48 38 51 1 1 0 10 4 3 2 5 4 1 1 7
Nucleus 0 0 0 0 0 2 1 4 3 1 0 0 0 0 0 21 13 8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 2 1 1 1 1 1 3 0 0 1 0 0 0 1 0 0 1 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 2
Vac/Vac Membrane 83 22 21 17 28 21 52 40 20 29 8 15 2 7 4 143 113 61 210 111 105 109 141 177
Unique Cell Count 231 128 122 165 277 283 444 576 379 448 272 401 193 328 436 302 230 132 278 163 144 166 221 278
Labelled Cell Count 264 145 128 170 292 302 481 618 426 509 307 449 199 338 447 367 275 151 278 163 144 166 221 278


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.7 5.1 4.9 5.3 4.8 4.4 4.6 4.5 4.1 4.0 4.1 5.8 5.1 4.9 7.8 9.7 11.0 5.5 5.3 5.6
Std Deviation (1e-4) 0.7 0.9 1.1 0.9 1.1 1.1 1.1 1.0 1.1 1.0 0.9 1.1 1.0 1.0 1.1 1.5 2.0 2.2 1.2 1.2 1.7
Intensity Change (Log2) – – – -0.05 0.05 -0.07 -0.19 -0.13 -0.18 -0.32 -0.34 -0.32 0.19 0.01 -0.04 0.62 0.93 1.11 0.13 0.06 0.14

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.8 5.9 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1239 4.3338 4.676 4.6374 3.6659 4.4571 0.3742 0.6519 1.5371 1.24 1.2185 1.4895 2.2538 3.1196 2.619 2.6686 2.9348 2.8836
Actin 0.0002 0.0017 0.0043 0.0008 0.0001 0.0001 0.0091 0.0026 0.0048 0.0001 0.0001 0.0018 0.0204 0.0001 0.0183 0.0025 0.0004 0.0033
Bud 0.0001 0.0042 0.0112 0.0004 0.0005 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0004 0.0001 0.005 0.0006 0.0002 0.0007
Bud Neck 0.0002 0.0048 0.0012 0.0006 0.0023 0.0058 0.0009 0.0003 0.002 0.0006 0.0005 0.0009 0.0025 0.0003 0.0008 0.0004 0.0018 0.002
Bud Periphery 0.0003 0.0013 0.0044 0.0007 0.0011 0.0005 0.0004 0.0002 0.0004 0.0005 0.0002 0.0004 0.0008 0.0003 0.0013 0.0007 0.0003 0.001
Bud Site 0.0003 0.0111 0.0021 0.001 0.0029 0.0012 0.0007 0.0015 0.0033 0.0006 0.001 0.0006 0.0038 0.001 0.005 0.0007 0.0017 0.0028
Cell Periphery 0.0029 0.0025 0.0037 0.0008 0.005 0.0016 0.0098 0.0018 0.0021 0.0091 0.0019 0.0012 0.0018 0.0008 0.0015 0.0007 0.0007 0.0012
Cytoplasm 0.3255 0.4258 0.4547 0.2846 0.434 0.4141 0.2915 0.2741 0.2725 0.2825 0.3604 0.2854 0.3063 0.414 0.4123 0.3424 0.2947 0.3895
Cytoplasmic Foci 0.008 0.0195 0.0132 0.0319 0.0239 0.0184 0.0106 0.0217 0.0389 0.0118 0.008 0.0257 0.0254 0.019 0.0216 0.0302 0.0142 0.016
Eisosomes 0.0001 0.0002 0.0001 0.0001 0 0 0.0005 0.0014 0.0002 0 0 0 0.0003 0 0.0002 0.0002 0 0.0001
Endoplasmic Reticulum 0.1816 0.1291 0.1595 0.0813 0.0771 0.1357 0.1132 0.0937 0.1389 0.2737 0.0847 0.1958 0.2091 0.1702 0.1882 0.1003 0.2564 0.2226
Endosome 0.0263 0.074 0.0736 0.2578 0.1111 0.1084 0.0235 0.0543 0.1589 0.061 0.0745 0.1151 0.0622 0.0736 0.0925 0.1285 0.0799 0.0761
Golgi 0.0009 0.0017 0.0017 0.0069 0.0011 0.0013 0.0023 0.0012 0.0089 0.0016 0.0012 0.0033 0.0054 0.001 0.0041 0.0019 0.0012 0.0029
Lipid Particles 0.0219 0.0064 0.0088 0.006 0.0061 0.0099 0.0051 0.0139 0.0196 0.0096 0.0036 0.0318 0.0212 0.0074 0.0127 0.0083 0.0055 0.01
Mitochondria 0.0007 0.0013 0.0038 0.0149 0.0016 0.0016 0.0005 0.0006 0.0039 0.0007 0.0004 0.0009 0.002 0.0006 0.0032 0.0019 0.0005 0.0013
None 0.344 0.1523 0.1067 0.1636 0.0175 0.1088 0.3109 0.4162 0.182 0.0484 0.2842 0.1261 0.2143 0.2042 0.0825 0.293 0.1416 0.1101
Nuclear Periphery 0.0141 0.0196 0.0243 0.0146 0.0177 0.0166 0.0224 0.0163 0.0207 0.0353 0.0068 0.0143 0.0298 0.0167 0.0216 0.0146 0.0329 0.0212
Nucleolus 0.0002 0.001 0.0006 0.0006 0.001 0.0011 0.0004 0.0003 0.0005 0.0002 0.0002 0.0004 0.001 0.0003 0.0005 0.0006 0.001 0.0006
Nucleus 0.0043 0.0108 0.0066 0.0079 0.0131 0.0205 0.0049 0.0031 0.0048 0.0034 0.0033 0.0041 0.0132 0.0058 0.0049 0.0045 0.0356 0.0053
Peroxisomes 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0014 0.0009 0.0001 0.0001 0.0119 0.0049 0.0001 0.0018 0.0005 0.0002 0.0015
Punctate Nuclear 0.0006 0.0027 0.001 0.0014 0.0008 0.0012 0.0034 0.0013 0.004 0.0005 0.0003 0.0011 0.0043 0.001 0.0018 0.0049 0.0048 0.0016
Vacuole 0.0653 0.1243 0.1138 0.1144 0.2723 0.1442 0.1837 0.0919 0.1245 0.2549 0.1653 0.1711 0.0673 0.0793 0.1138 0.0568 0.1207 0.1238
Vacuole Periphery 0.0023 0.0053 0.0046 0.0092 0.0107 0.0086 0.0061 0.002 0.0081 0.0052 0.003 0.0077 0.0037 0.0041 0.0064 0.0058 0.0056 0.0065

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.6845 55.1568 38.271 42.7985 31.168 49.6537 56.1312 54.2607 51.2888 47.9695
Translational Efficiency 0.6261 0.6075 0.7651 0.6104 0.7074 0.5583 0.6321 0.551 0.5121 0.4523

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
877 1019 959 1323 342 1168 2056 1279 1219 2187 3015 2602

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 669.26 803.16 858.72 836.59 777.77 775.04 810.71 866.78 699.70 788.14 825.98 851.43
Standard Deviation 77.01 123.42 105.68 111.00 94.78 99.08 108.65 131.84 95.72 111.97 110.01 122.63
Intensity Change Log 2 — 0.263121 0.359621 0.321954 — -0.005073 0.059842 0.156322 — 0.125185 0.206261 0.235297

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000025 0.000312 0.000296 0.000606 0.000101 0.000610 0.000760 0.001726 0.000046 0.000471 0.000612 0.001157
Bud Neck 0.007383 0.014791 0.003560 0.007755 0.004960 0.014500 0.005926 0.015871 0.006703 0.014635 0.005173 0.011745
Bud Site 0.002137 0.013585 0.004114 0.023470 0.002707 0.011497 0.009811 0.140931 0.002297 0.012470 0.007999 0.081207
Cell Periphery 0.000459 0.000249 0.000221 0.000277 0.000317 0.000237 0.000369 0.004616 0.000419 0.000243 0.000322 0.002410
Cytoplasm 0.466654 0.204599 0.485456 0.462178 0.344246 0.167538 0.439633 0.451284 0.432312 0.184806 0.454208 0.456823
Cytoplasmic Foci 0.133988 0.142586 0.036287 0.043018 0.176787 0.118250 0.054153 0.038251 0.145996 0.129589 0.048470 0.040675
Eisosomes 0.000219 0.000136 0.000058 0.000078 0.000164 0.000124 0.000163 0.000084 0.000204 0.000130 0.000129 0.000081
Endoplasmic Reticulum 0.013643 0.039440 0.018004 0.014131 0.061775 0.041387 0.085774 0.006822 0.027147 0.040480 0.064218 0.010538
Endosome 0.016714 0.068343 0.007990 0.010027 0.034459 0.068507 0.009806 0.010634 0.021693 0.068431 0.009228 0.010325
Golgi 0.000884 0.004617 0.000143 0.001656 0.000920 0.005144 0.000636 0.002131 0.000894 0.004898 0.000479 0.001889
Lipid Particles 0.017563 0.017185 0.005173 0.005994 0.036779 0.019280 0.018630 0.004242 0.022954 0.018304 0.014350 0.005133
Mitochondria 0.000464 0.003200 0.000298 0.002548 0.000570 0.006013 0.000857 0.008083 0.000494 0.004702 0.000679 0.005269
Mitotic Spindle 0.000099 0.003920 0.002254 0.028452 0.000105 0.003691 0.001434 0.044349 0.000101 0.003798 0.001695 0.036266
None 0.005858 0.000871 0.002491 0.004025 0.003181 0.000903 0.002451 0.010028 0.005107 0.000888 0.002464 0.006976
Nuclear Periphery 0.002915 0.001287 0.006177 0.002766 0.006351 0.001556 0.014494 0.002336 0.003879 0.001431 0.011848 0.002555
Nuclear Periphery Foci 0.001636 0.002356 0.022342 0.011255 0.012606 0.001386 0.019818 0.005827 0.004714 0.001838 0.020621 0.008587
Nucleolus 0.001130 0.001062 0.000560 0.000734 0.001401 0.000966 0.000618 0.001247 0.001206 0.001011 0.000600 0.000986
Nucleus 0.161001 0.026226 0.278388 0.130875 0.096654 0.028322 0.190459 0.103220 0.142948 0.027345 0.218427 0.117281
Peroxisomes 0.001483 0.004541 0.000443 0.001381 0.001401 0.004866 0.000878 0.001203 0.001460 0.004715 0.000739 0.001293
Vacuole 0.164925 0.443569 0.125496 0.246925 0.212627 0.495834 0.142954 0.141538 0.178308 0.471482 0.137401 0.195122
Vacuole Periphery 0.000818 0.007125 0.000249 0.001849 0.001889 0.009389 0.000376 0.005578 0.001118 0.008334 0.000336 0.003682

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.80 -13.81 -3.40 -1.22 -1.14 -2.79 -3.62 -5.53 -2.75 -2.30 -4.21 -4.69 -6.60 -2.85 -1.86
Bud Neck -4.58 3.84 -0.61 4.22 -4.85 -5.96 -0.86 -6.35 -0.24 -7.39 -7.14 1.74 -5.01 2.92 -7.95
Bud Site -6.69 -2.43 -7.13 -1.65 -6.02 -6.18 -4.38 -20.91 -19.02 -19.32 -9.02 -5.04 -20.89 -17.11 -18.59
Cell Periphery 4.98 6.21 6.30 1.81 1.25 2.80 -1.00 -8.31 -8.51 -8.23 5.79 3.61 -7.28 -8.21 -7.88
Cytoplasm 24.95 1.05 6.14 -18.29 4.83 12.85 -6.27 -6.39 -27.50 -0.79 30.22 -0.74 1.59 -32.35 2.70
Cytoplasmic Foci -1.10 20.17 20.60 20.72 0.22 7.21 16.64 19.27 18.30 7.29 3.52 23.88 27.49 27.55 6.99
Eisosomes 5.25 14.18 13.65 5.12 -1.81 2.95 0.61 7.69 3.75 6.70 6.67 7.19 14.95 6.32 6.80
Endoplasmic Reticulum -6.37 -1.35 2.26 8.47 4.64 2.17 -1.81 6.53 9.63 17.16 -3.47 -7.81 6.80 12.77 17.30
Endosome -16.96 7.42 7.85 21.20 0.86 -10.37 11.41 11.55 23.36 1.58 -21.97 11.90 12.56 31.62 2.31
Golgi -5.19 2.58 0.27 5.45 -2.56 -5.10 2.37 -1.99 4.12 -3.03 -6.94 2.06 -0.90 6.56 -3.43
Lipid Particles 0.34 8.91 9.30 8.98 1.30 4.50 5.00 8.98 12.31 13.79 2.81 5.84 12.92 15.05 13.43
Mitochondria -4.87 4.13 -1.57 2.08 -1.83 -5.04 -1.33 -3.72 0.20 -3.52 -6.65 -1.45 -4.06 1.21 -3.80
Mitotic Spindle -2.73 -2.89 -7.30 -5.33 -5.89 -3.00 -4.34 -10.60 -9.07 -10.11 -4.05 -4.90 -12.79 -10.34 -11.73
None 3.52 2.57 1.84 -3.47 -1.55 1.49 0.61 -2.97 -7.23 -6.46 3.81 2.61 -0.31 -7.77 -5.90
Nuclear Periphery 3.24 -4.27 -0.90 -8.66 4.62 4.39 -5.25 3.29 -1.94 10.20 5.06 -8.85 1.74 -5.43 11.09
Nuclear Periphery Foci -1.54 -10.84 -6.75 -5.63 7.28 6.97 -3.18 4.53 -4.21 10.00 5.29 -13.38 -1.76 -7.09 12.01
Nucleolus 0.51 3.48 2.21 1.72 -1.44 1.42 2.83 0.56 -0.78 -2.24 1.31 4.40 1.36 0.25 -2.58
Nucleus 23.43 -12.60 2.20 -23.69 14.98 8.71 -10.71 -1.84 -18.87 13.30 24.31 -12.72 2.49 -30.04 18.08
Peroxisomes -3.98 2.31 1.26 5.56 -1.66 -4.73 4.04 3.11 5.42 -0.25 -5.59 2.25 1.69 7.67 -1.03
Vacuole -24.05 0.70 -16.15 8.74 -18.08 -20.05 4.15 1.64 26.77 -3.29 -33.43 2.34 -10.65 24.36 -15.76
Vacuole Periphery -13.02 9.93 -0.49 11.27 -2.76 -9.69 6.01 -2.30 4.78 -4.16 -15.70 8.61 -2.89 9.26 -4.72
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant
Localization
Cell Percentages ER (20%), vacuole (17%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Alg3

Alg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available