Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0.09 0.05 0.08 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.33 0.68 0.44 0.51 0.52 0.62 0.75 0.61 – 0.58 0.63 0.32 0.41 0.5 0.17 0.28 0.22 0.24 0.23 0.29 0.2 0.26 0.35
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.07 0.09 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0.1 0.08 0.17 0.15 0.1 0.06 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.15 0 0.24 0 0.26 0.24 0.13 0.41 – 0.47 0.42 0.22 0.06 0.05 0 0.06 0.15 0.07 0.06 0.09 0.1 0.2 0.12
Nucleus 0.13 0.19 0.11 0.21 0.19 0.12 0.07 0.06 0 – 0.05 0 0.26 0.26 0.22 0.38 0.28 0.24 0.18 0.25 0.12 0.07 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.09 0.23 0.1 0.12 0.06 0.05 – 0 0 0 0 0 0.05 0.07 0 0.17 0.11 0.11 0.13 0.13 0.1
Vac/Vac Membrane 0.27 0.36 0.06 0 0 0 0 0 0 – 0 0 0.21 0.23 0.16 0.28 0.25 0.33 0.17 0.21 0.21 0.32 0.21 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 1 2 1 2 2
Bud 1 0 1 1 2 2 5 1 4 – 14 4 2 0 2 0 0 1 2 4 1 3 6 9
Bud Neck 0 0 13 3 13 10 25 19 11 – 6 10 0 0 0 0 0 0 1 4 2 2 4 6
Bud Site 1 0 0 0 0 1 3 1 3 – 6 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 71 66 101 40 75 109 201 271 201 – 146 149 72 82 128 24 25 17 55 62 77 26 88 88
Endoplasmic Reticulum 2 3 2 1 0 0 2 0 1 – 0 0 0 0 1 7 6 7 2 0 3 1 7 5
Endosome 6 9 2 3 4 4 1 1 0 – 0 0 8 21 21 24 13 8 13 12 9 2 11 7
Golgi 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 3 1 2 1 0 1 0 2 1
Mitochondria 34 30 6 22 3 55 79 46 136 – 118 100 50 12 13 5 5 12 15 17 22 13 66 29
Nucleus 20 38 16 19 28 25 24 22 14 – 13 10 60 52 56 54 25 19 41 67 32 9 14 6
Nuclear Periphery 0 1 0 0 0 0 0 1 0 – 0 1 0 0 0 0 1 0 3 1 0 1 1 1
Nucleolus 0 1 0 0 0 0 0 0 0 – 1 0 7 3 2 2 3 1 6 6 3 2 2 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0
SpindlePole 1 1 5 8 34 20 39 23 15 – 11 9 6 2 1 7 6 1 38 31 30 17 42 26
Vac/Vac Membrane 41 72 9 2 5 9 6 1 3 – 4 2 48 45 41 40 22 26 38 56 56 41 71 57
Unique Cell Count 153 199 149 90 146 209 326 359 332 251 237 228 200 254 142 88 79 231 275 266 130 335 252
Labelled Cell Count 177 223 157 99 164 235 386 386 388 319 285 253 217 265 166 109 94 231 275 266 130 335 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.1 4.5 3.8 4.7 3.7 3.7 4.0 3.1 – 2.9 3.3 4.2 4.3 4.3 7.1 7.0 6.7 3.9 4.0 4.4
Std Deviation (1e-4) 0.5 0.7 0.9 0.5 1.2 1.1 0.9 1.2 0.8 – 0.8 0.8 1.0 1.0 1.2 1.9 1.8 2.1 1.7 1.5 1.9
Intensity Change (Log2) – – – -0.23 0.06 -0.29 -0.28 -0.15 -0.52 – -0.64 -0.44 -0.11 -0.07 -0.05 0.66 0.63 0.57 -0.19 -0.16 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.0 4.2 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9841 -0.9425 -0.7551 -1.0731 -0.9913 -0.8585 -0.0873 -0.2366 -0.2981 -0.2832 0.2546 -0.0379 -0.5321 -0.6151 -0.6356 -0.5258 -0.3284 -0.6503
Actin 0.0331 0.0006 0.0251 0.0076 0.0129 0.008 0.0361 0.0004 0.0161 0.0003 0.01 0.0053 0.0489 0.0005 0.004 0.0163 0.0671 0.0009
Bud 0.0008 0.0015 0.0008 0.0013 0.0007 0.0002 0.0019 0.0002 0.0003 0 0.0003 0.0011 0.0014 0.0003 0.0002 0.0002 0.0042 0.0001
Bud Neck 0.0086 0.0007 0.0018 0.0015 0.0026 0.0056 0.0027 0.0018 0.0013 0.0006 0.0015 0.0157 0.0157 0.0012 0.0034 0.0006 0.0017 0.0057
Bud Periphery 0.0013 0.0004 0.001 0.0033 0.0006 0.0004 0.0039 0.0001 0.0003 0 0.0005 0.0022 0.0018 0.0003 0.0003 0.0002 0.0074 0.0002
Bud Site 0.0067 0.0028 0.0043 0.0024 0.0132 0.0019 0.0099 0.0024 0.0011 0.0001 0.0012 0.0017 0.0197 0.0028 0.0121 0.0004 0.0194 0.0003
Cell Periphery 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002 0.0008 0.0001 0.0001 0 0.0002 0.0001 0.0008 0.0002 0.0005 0 0.0007 0.0001
Cytoplasm 0.0814 0.0961 0.0667 0.0123 0.0678 0.0618 0.0822 0.0718 0.0463 0.0306 0.0569 0.056 0.0815 0.1002 0.0665 0.089 0.1209 0.0896
Cytoplasmic Foci 0.0498 0.0139 0.014 0.0139 0.0146 0.0174 0.0408 0.0068 0.0111 0.0006 0.0434 0.0155 0.0578 0.0193 0.0146 0.0115 0.0127 0.0049
Eisosomes 0.0008 0.0001 0.0003 0.0001 0.0001 0.0002 0.0003 0 0.0001 0 0.0002 0.0001 0.0007 0.0001 0.0003 0.0001 0.0008 0
Endoplasmic Reticulum 0.0119 0.0057 0.0073 0.0021 0.0077 0.005 0.0123 0.005 0.0026 0.0011 0.0084 0.0018 0.0052 0.0083 0.0075 0.004 0.0052 0.0035
Endosome 0.04 0.014 0.0128 0.0288 0.0259 0.0185 0.054 0.0059 0.0163 0.0009 0.0413 0.0107 0.0231 0.0059 0.0176 0.0043 0.035 0.0071
Golgi 0.0094 0.0004 0.0039 0.0037 0.0083 0.0045 0.0174 0.0004 0.0117 0 0.0229 0.0038 0.0063 0.0003 0.0046 0.002 0.0259 0.0013
Lipid Particles 0.0418 0.0015 0.0063 0.0092 0.0029 0.0112 0.0258 0.0012 0.0122 0.0001 0.0719 0.0033 0.0218 0.0014 0.0147 0.0023 0.0152 0.0129
Mitochondria 0.0097 0.0008 0.0068 0.0076 0.0033 0.0138 0.0156 0.0006 0.0106 0.0003 0.0499 0.0049 0.0023 0.0006 0.0028 0.0014 0.0944 0.0009
None 0.2063 0.1592 0.1718 0.0129 0.0382 0.1192 0.1182 0.1269 0.1179 0.1165 0.0408 0.0563 0.1721 0.2675 0.2078 0.121 0.076 0.1285
Nuclear Periphery 0.0906 0.044 0.0503 0.0192 0.0722 0.0456 0.069 0.0477 0.0584 0.0641 0.0318 0.0232 0.0303 0.0463 0.0444 0.1304 0.0142 0.0248
Nucleolus 0.0111 0.0163 0.0133 0.0159 0.0266 0.0296 0.0155 0.0368 0.0187 0.0085 0.0173 0.0317 0.0178 0.0287 0.0147 0.0056 0.0514 0.0326
Nucleus 0.2377 0.5158 0.4734 0.6477 0.5937 0.4861 0.3724 0.5666 0.5331 0.7493 0.4931 0.6409 0.2833 0.3976 0.445 0.4946 0.3372 0.5998
Peroxisomes 0.0202 0.0008 0.003 0.0027 0.001 0.0091 0.017 0.0017 0.0055 0 0.0279 0.0018 0.0332 0.0004 0.0077 0.0379 0.0414 0.001
Punctate Nuclear 0.1247 0.1215 0.1334 0.2017 0.1026 0.1578 0.0827 0.1205 0.1302 0.0259 0.075 0.1214 0.1692 0.1133 0.1275 0.0764 0.0605 0.0836
Vacuole 0.01 0.003 0.0022 0.0036 0.0027 0.0022 0.0161 0.0024 0.0042 0.0003 0.0032 0.0019 0.0061 0.0041 0.0027 0.0007 0.0064 0.0016
Vacuole Periphery 0.0036 0.0007 0.0013 0.0024 0.0021 0.0016 0.0054 0.0007 0.0018 0.0007 0.0023 0.0006 0.0012 0.0007 0.0008 0.0013 0.0025 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4494 12.9957 5.4646 18.8075 5.6546 7.2648 13.797 14.0419 10.1845 16.8748
Translational Efficiency 0.5661 0.4277 1.0942 0.2594 0.747 0.8988 0.5478 0.417 0.7595 0.4339

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1433 156 251 322 1942 1728 1533 63 3375 1884 1784 385

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 692.20 826.13 996.91 936.92 646.30 765.65 880.40 1012.76 665.79 770.66 896.79 949.33
Standard Deviation 76.55 108.88 111.49 138.15 80.30 93.24 109.96 151.25 81.93 96.09 117.39 143.15
Intensity Change Log 2 — 0.255180 0.526274 0.436737 — 0.244481 0.445955 0.648016 — 0.250024 0.488050 0.542620

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000070 0.000683 0.003156 0.001444 0.000061 0.000415 0.000600 0.000918 0.000065 0.000437 0.000959 0.001358
Bud Neck 0.004836 0.010577 0.011634 0.027097 0.005845 0.015475 0.007945 0.090645 0.005417 0.015069 0.008464 0.037496
Bud Site 0.003825 0.004884 0.009151 0.019955 0.000977 0.006544 0.002006 0.013580 0.002186 0.006407 0.003011 0.018912
Cell Periphery 0.000100 0.000152 0.000209 0.000448 0.000127 0.000240 0.000126 0.000075 0.000115 0.000232 0.000138 0.000387
Cytoplasm 0.302380* 0.180441* 0.020699 0.026512 0.340288* 0.262472* 0.057829 0.007865 0.324192* 0.255680* 0.052605 0.023461
Cytoplasmic Foci 0.043726 0.120658 0.001070 0.011851 0.087714 0.148893 0.001163 0.000126 0.069037 0.146555 0.001150 0.009933
Eisosomes 0.000040 0.000123 0.000104 0.000034 0.000063 0.000102 0.000027 0.000044 0.000053 0.000104 0.000037 0.000035
Endoplasmic Reticulum 0.000652 0.004290 0.021779 0.005060 0.001006 0.002730 0.004940 0.004894 0.000856 0.002860 0.007309 0.005033
Endosome 0.001501 0.016776 0.002425 0.019651 0.002983 0.011559 0.000877 0.000824 0.002354 0.011991 0.001095 0.016570
Golgi 0.000555 0.002090 0.000357 0.046654 0.000809 0.004846 0.001097 0.000221 0.000701 0.004618 0.000993 0.039056
Lipid Particles 0.003830 0.013837 0.002892 0.001481 0.010695 0.026438 0.000554 0.000249 0.007780 0.025395 0.000883 0.001279
Mitochondria 0.002516 0.009699 0.001900 0.076407 0.004330 0.021463 0.001515 0.005074 0.003560 0.020489 0.001569 0.064734
Mitotic Spindle 0.003644 0.016674 0.018207 0.317212* 0.002642 0.024634 0.008360 0.219823* 0.003068 0.023975 0.009745 0.301276*
None 0.012964 0.014633 0.005938 0.020648 0.015716 0.007100 0.010681 0.008889 0.014547 0.007724 0.010013 0.018724
Nuclear Periphery 0.001603 0.004620 0.002955 0.003805 0.002507 0.005235 0.002510 0.001420 0.002123 0.005184 0.002573 0.003415
Nuclear Periphery Foci 0.001069 0.004944 0.024606 0.004653 0.001725 0.003267 0.004077 0.001215 0.001446 0.003406 0.006965 0.004091
Nucleolus 0.006851 0.011181 0.015627 0.009570 0.007262 0.011364 0.008413 0.012233 0.007088 0.011349 0.009428 0.010006
Nucleus 0.602672* 0.531277* 0.834686* 0.366698* 0.501478* 0.407363* 0.882616* 0.625769* 0.544445* 0.417623* 0.875872* 0.409091*
Peroxisomes 0.002210 0.008746 0.001233 0.000942 0.003338 0.015612 0.000195 0.000402 0.002859 0.015043 0.000341 0.000854
Vacuole 0.004716 0.042656 0.020908 0.024808 0.010073 0.022139 0.004339 0.005112 0.007798 0.023838 0.006670 0.021585
Vacuole Periphery 0.000240 0.001059 0.000465 0.015070 0.000362 0.002109 0.000130 0.000621 0.000310 0.002022 0.000178 0.012706

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.40 -12.59 -12.86 -7.06 5.05 -2.95 -21.05 -5.41 -4.42 -2.58 -3.38 -21.58 -13.99 -10.34 -4.43
Bud Neck -2.24 -8.18 -7.57 -5.07 -2.35 -6.96 -7.37 -2.68 -1.98 -1.98 -7.82 -9.75 -7.49 -4.65 -4.34
Bud Site -0.76 -3.35 -4.03 -3.61 -0.43 -6.29 -9.12 -3.15 -1.61 -2.20 -4.69 -5.42 -5.06 -3.53 -3.69
Cell Periphery -2.81 -7.85 -2.36 -0.88 1.52 -1.53 -0.20 0.05 1.52 0.20 -1.79 -3.37 -2.01 0.54 -1.05
Cytoplasm 7.47 38.65 37.06 8.81 -2.48 9.17 45.01 45.98 36.01 6.20 9.44 55.94 57.60 37.80 4.33
Cytoplasmic Foci -4.84 16.47 13.03 8.29 -1.25 -10.56 27.33 27.57 33.67 3.04 -15.38 31.51 25.16 31.87 -1.33
Eisosomes -5.90 -8.91 -4.12 3.89 6.08 -9.14 5.37 0.01 2.84 -1.34 -13.45 -1.80 -2.07 6.30 -0.99
Endoplasmic Reticulum -5.12 -14.40 -11.84 -5.20 7.02 -8.05 -22.25 -3.80 -3.09 -0.97 -9.99 -24.77 -12.12 -9.50 -1.03
Endosome -3.90 -5.12 -2.58 2.94 -0.54 -10.15 6.46 6.19 12.91 1.23 -11.73 4.15 -1.53 6.66 -2.50
Golgi -1.87 -1.63 -2.28 -1.85 -2.17 -5.19 0.35 2.35 6.15 0.82 -5.59 0.01 -2.20 -0.83 -2.18
Lipid Particles -3.66 0.33 7.16 5.04 5.48 -9.77 13.24 12.62 17.75 0.15 -12.38 14.14 14.07 18.24 1.35
Mitochondria -1.15 0.00 -2.68 -1.13 -2.70 -9.35 3.56 0.55 9.47 -2.43 -9.86 2.75 -2.49 1.19 -2.80
Mitotic Spindle -1.31 -5.45 -11.32 -10.28 -7.69 -7.44 -9.06 -4.22 -3.68 -3.38 -7.55 -10.46 -12.11 -10.85 -9.88
None 0.02 7.33 -0.78 -0.42 -4.07 6.63 4.54 1.47 -1.16 -0.54 6.93 5.92 -0.19 -3.04 -2.75
Nuclear Periphery -3.60 -6.01 -7.28 -2.24 -3.26 -5.45 -5.81 -1.83 2.25 1.78 -6.61 -7.76 -7.15 -2.16 -3.04
Nuclear Periphery Foci -2.20 -5.81 -2.44 0.28 5.17 -1.78 -4.99 0.94 2.80 5.54 -3.12 -7.76 -2.09 -0.71 4.56
Nucleolus -1.23 -5.79 -3.78 -1.98 1.73 -2.48 -7.72 -0.42 0.67 4.63 -2.44 -8.94 -4.06 -3.12 1.33
Nucleus 1.81 -5.44 1.65 -0.43 5.34 9.50 -35.09 -4.44 -6.54 2.76 14.16 -33.08 -2.31 -8.57 11.35
Peroxisomes -3.21 -0.23 2.94 4.01 3.44 -10.76 10.22 9.39 14.15 -0.52 -11.73 9.88 6.35 13.85 -0.53
Vacuole -5.24 -7.50 -5.66 1.04 -0.59 -8.70 0.62 -1.23 2.91 -1.43 -11.51 -4.01 -5.30 -1.34 -4.24
Vacuole Periphery -5.07 -4.96 -2.62 -1.81 -2.20 -7.47 5.64 0.62 7.23 -1.34 -8.00 2.01 -2.54 -0.52 -2.61
Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
Localization
Cell Percentages nucleus (50%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cdc27

Cdc27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available