Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0.05 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.87 0.98 0.98 0.96 0.98 0.9 0.95 – 0.82 0.85 0.76 0.77 1.0 1.0 0.99 – 0.97 – 0.65 0.86 0.74 0.83 0.84 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.07 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.27 0 0 0 0 0.15 0 – 0.27 0.19 0.37 0.32 0 0 0 – 0 – 0.18 0.06 0.13 0.07 0.06 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 3 1 3 8
Bud 0 1 0 2 0 2 0 – 5 3 5 3 0 1 0 – 0 – 1 0 0 5 10 12
Bud Neck 0 2 12 2 10 2 9 – 10 10 2 5 0 0 0 – 1 – 0 1 0 1 2 3
Bud Site 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 3 1 3 0 0 1 – 0 0 0 3 0 2 1 – 3 – 0 1 2 1 2 5
Cytoplasm 77 204 208 188 197 181 320 – 202 200 154 169 392 572 561 – 110 – 63 178 151 252 393 382
Endoplasmic Reticulum 0 0 0 1 0 0 0 – 0 0 0 1 0 1 1 – 8 – 2 0 0 2 2 1
Endosome 0 0 1 0 0 0 2 – 0 1 1 0 0 0 0 – 2 – 2 2 3 1 4 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 2 1 1 2 1 2
Mitochondria 24 7 1 8 0 31 13 – 67 44 75 70 0 1 0 – 2 – 16 12 26 21 26 27
Nucleus 0 0 1 2 1 3 5 – 4 5 3 2 0 0 0 – 1 – 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 – 0 0 0 0 0 0 1 – 0 – 0 0 0 0 0 0
Nucleolus 2 0 0 0 0 2 1 – 1 0 1 0 0 0 1 – 0 – 0 0 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 1 2 3 0 6 – 5 5 4 5 0 0 1 – 0 – 2 0 2 2 8 6
Vac/Vac Membrane 1 0 0 0 0 0 5 – 0 2 0 1 0 0 1 – 0 – 2 2 0 2 2 8
Unique Cell Count 89 208 212 195 200 201 337 246 235 202 219 392 574 565 113 97 208 205 304 468 470
Labelled Cell Count 105 217 225 209 211 221 363 294 270 247 259 392 577 567 127 97 208 205 304 468 470


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.4 4.3 4.5 5.2 3.6 4.5 – 3.6 4.2 3.6 3.6 4.1 4.1 4.7 4.8 5.0 4.7 3.9 4.0 4.1
Std Deviation (1e-4) 0.8 0.7 0.8 1.9 2.3 1.7 2.3 – 1.6 2.5 1.3 1.2 0.9 1.1 1.4 1.6 1.5 1.7 1.8 1.7 1.8
Intensity Change (Log2) – – – 0.05 0.25 -0.28 0.05 – -0.26 -0.03 -0.27 -0.26 -0.07 -0.09 0.11 0.16 0.22 0.13 -0.16 -0.13 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.758 -1.1353 -0.9278 -1.5172 -1.4257 -0.9149 -0.7728 -0.1059 -0.225 -0.3863 -0.5286 -0.2594 1.5461 1.3796 1.6387 2.2241 1.8105 1.3857
Actin 0.0369 0.0001 0.0118 0.0008 0.0009 0.0053 0.0152 0.0009 0.0024 0.0008 0.0001 0.0003 0.0389 0.0002 0.0244 0.0334 0.0181 0.0067
Bud 0.0017 0.0001 0.0022 0.0008 0.0004 0.0007 0.0001 0.0004 0.0061 0.0001 0 0.0002 0.0011 0.0003 0.0008 0.0008 0.0003 0.0004
Bud Neck 0.0073 0.0001 0.0101 0.0001 0.0002 0.0003 0.0013 0.0002 0.0065 0.0002 0.0001 0.0004 0.0072 0.0002 0.0019 0.0014 0.0006 0.001
Bud Periphery 0.0042 0.0001 0.0059 0.0012 0.0008 0.0054 0.0001 0.0009 0.004 0.0001 0.0001 0.0004 0.0021 0.0001 0.0018 0.0015 0.0011 0.0014
Bud Site 0.0064 0.0016 0.0063 0.0008 0.0006 0.0001 0.0004 0.0006 0.0077 0.0001 0.0001 0.0001 0.0069 0.0051 0.0044 0.002 0.0007 0.0002
Cell Periphery 0.0006 0 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0.0007 0.0001 0.0002 0.0001 0.0003 0.0013
Cytoplasm 0.0091 0.0181 0.0167 0.0501 0.0129 0.0265 0.0449 0.0855 0.0745 0.076 0.0517 0.1052 0.0274 0.0286 0.0137 0.0163 0.0108 0.0318
Cytoplasmic Foci 0.0201 0.0024 0.0104 0.0365 0.0148 0.0049 0.0038 0.0042 0.0157 0.0057 0.0261 0.0113 0.0158 0.0089 0.0113 0.021 0.007 0.0038
Eisosomes 0.001 0.0001 0.0003 0.0012 0.0001 0.0001 0.0004 0.0001 0.0001 0.0009 0.0001 0.0001 0.0008 0.0001 0.0005 0.0003 0.0007 0.0002
Endoplasmic Reticulum 0.0027 0.0001 0.0016 0.0003 0.0011 0.0004 0.0007 0.0005 0.0007 0.0004 0.0002 0.0004 0.0021 0.0002 0.0004 0.0016 0.0052 0.0002
Endosome 0.0124 0.0001 0.012 0.0019 0.0095 0.0022 0.0015 0.0005 0.0101 0.0007 0.0324 0.0042 0.0113 0.0005 0.0011 0.0031 0.0286 0.001
Golgi 0.0045 0 0.0034 0.0006 0.0017 0.0011 0.0008 0.0001 0.0008 0.0001 0.0019 0.0001 0.0033 0.0004 0.0014 0.003 0.0099 0.0006
Lipid Particles 0.0129 0.0001 0.0048 0.0015 0.0181 0.0017 0.0008 0.0001 0.0016 0.0003 0.0017 0.0002 0.0135 0.0049 0.0024 0.0045 0.0195 0.0016
Mitochondria 0.0046 0.0001 0.0122 0.001 0.0021 0.0006 0.0004 0.0005 0.0013 0.0004 0.0091 0.0002 0.0033 0.0028 0.0098 0.0011 0.0082 0.0006
None 0.836 0.9751 0.8856 0.883 0.8889 0.9406 0.9265 0.9014 0.8519 0.9093 0.8719 0.8713 0.8365 0.9394 0.9131 0.8708 0.7757 0.9468
Nuclear Periphery 0.0109 0.0001 0.0029 0.0004 0.0139 0.0041 0.0004 0.0006 0.0007 0.0004 0.0001 0.0002 0.0083 0.0001 0.0004 0.0016 0.043 0.0003
Nucleolus 0.0015 0.0003 0.0006 0.0004 0.0011 0.0003 0.0001 0.0001 0.0011 0.0005 0.0001 0.0002 0.001 0.0004 0.0003 0.0003 0.001 0.0001
Nucleus 0.0115 0.0004 0.0015 0.0006 0.0059 0.001 0.0006 0.0009 0.0056 0.0009 0.0002 0.0009 0.003 0.0006 0.0002 0.0007 0.0053 0.0002
Peroxisomes 0.0069 0 0.0032 0.0131 0.0162 0.0005 0.001 0.0003 0.0022 0.0002 0.0006 0.0002 0.0108 0.0046 0.0087 0.0218 0.0484 0.001
Punctate Nuclear 0.0057 0.0008 0.0067 0.0048 0.0037 0.0037 0.0005 0.0017 0.0042 0.0023 0.0002 0.0027 0.0031 0.0022 0.0027 0.0141 0.0098 0.0007
Vacuole 0.002 0.0001 0.0009 0.0006 0.0041 0.0002 0.0004 0.0003 0.0024 0.0003 0.0023 0.0014 0.0018 0.0003 0.0002 0.0005 0.0021 0.0001
Vacuole Periphery 0.0013 0 0.0005 0.0001 0.003 0.0002 0.0001 0.0001 0.0003 0.0001 0.0009 0.0001 0.001 0 0.0001 0.0002 0.0034 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4641 25.86 27.4132 25.2469 17.4138 22.7167 30.8162 25.841 33.8865 32.4072
Translational Efficiency 2.7739 1.5928 1.4639 2.0206 2.3233 2.4973 1.8674 2.7657 1.7273 1.6695

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
251 1701 862 102 1161 2220 169 184 1412 3921 1031 286

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.38 665.76 832.33 846.85 649.70 719.12 832.19 889.95 650.00 695.97 832.31 874.58
Standard Deviation 73.34 99.56 91.95 86.60 70.58 84.07 107.60 112.09 71.08 94.87 94.70 105.75
Intensity Change Log 2 — 0.031503 0.353656 0.378607 — 0.146459 0.357139 0.453951 — 0.090051 0.355397 0.416722

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000339 0.000426 0.000146 0.000117 0.000123 0.000406 0.000367 0.000129 0.000161 0.000415 0.000183 0.000125
Bud Neck 0.039477 0.037760 0.002549 0.001324 0.008053 0.053943 0.000999 0.002875 0.013639 0.046922 0.002295 0.002322
Bud Site 0.004427 0.018108 0.001759 0.001739 0.002576 0.020332 0.001288 0.008813 0.002905 0.019367 0.001682 0.006290
Cell Periphery 0.000277 0.000154 0.000118 0.000164 0.000182 0.000406 0.000159 0.000455 0.000199 0.000296 0.000125 0.000351
Cytoplasm 0.311927* 0.370999* 0.327532* 0.345794* 0.515202* 0.404114* 0.345043* 0.318788* 0.479067* 0.389748* 0.330403* 0.328419*
Cytoplasmic Foci 0.381656* 0.304165* 0.010798 0.052041 0.219597* 0.200821* 0.020073 0.078022 0.248405* 0.245653* 0.012319 0.068756
Eisosomes 0.000597 0.000188 0.000043 0.000117 0.000149 0.000243 0.000072 0.000194 0.000228 0.000219 0.000048 0.000166
Endoplasmic Reticulum 0.000324 0.000673 0.002541 0.001858 0.000411 0.001203 0.004628 0.001254 0.000395 0.000973 0.002883 0.001470
Endosome 0.021127 0.032872 0.002326 0.008842 0.006964 0.019597 0.002923 0.004563 0.009481 0.025356 0.002424 0.006089
Golgi 0.004088 0.009355 0.000066 0.004194 0.001596 0.004127 0.000077 0.000161 0.002039 0.006395 0.000068 0.001600
Lipid Particles 0.020788 0.010147 0.001672 0.012681 0.008667 0.010395 0.002798 0.003401 0.010822 0.010287 0.001856 0.006710
Mitochondria 0.014510 0.024446 0.000491 0.004629 0.003569 0.015585 0.000306 0.000370 0.005514 0.019429 0.000461 0.001889
Mitotic Spindle 0.002506 0.003367 0.001664 0.005860 0.000999 0.007048 0.007772 0.001454 0.001267 0.005451 0.002665 0.003025
None 0.002887 0.003927 0.004268 0.005836 0.004552 0.006069 0.008241 0.004347 0.004256 0.005140 0.004919 0.004878
Nuclear Periphery 0.000309 0.000432 0.001629 0.001285 0.000413 0.000681 0.001322 0.000890 0.000395 0.000573 0.001579 0.001031
Nuclear Periphery Foci 0.000160 0.000420 0.001107 0.001532 0.000307 0.000304 0.002384 0.000626 0.000281 0.000355 0.001316 0.000949
Nucleolus 0.001481 0.002384 0.000771 0.000585 0.001141 0.002100 0.000794 0.000924 0.001202 0.002223 0.000775 0.000803
Nucleus 0.115653 0.089360 0.599003* 0.323699* 0.192795* 0.172279* 0.528522* 0.453310* 0.179082* 0.136307* 0.587450* 0.407085*
Peroxisomes 0.044205 0.020395 0.000298 0.001423 0.004098 0.011072 0.000367 0.000963 0.011228 0.015117 0.000309 0.001127
Vacuole 0.032151 0.067293 0.041090 0.226058* 0.027842 0.067089 0.071728 0.118052 0.028608 0.067178 0.046112 0.156571
Vacuole Periphery 0.001109 0.003127 0.000130 0.000222 0.000765 0.002186 0.000138 0.000408 0.000826 0.002595 0.000131 0.000342

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.94 1.55 1.68 3.91 0.50 -3.08 -3.59 -1.76 1.71 2.35 -3.78 -1.84 -1.22 3.14 0.60
Bud Neck 0.28 6.07 6.29 18.23 1.76 -17.48 7.92 4.75 19.39 -1.19 -15.87 7.97 8.16 25.50 0.35
Bud Site -8.03 2.35 2.07 10.20 -0.48 -10.66 0.96 -1.18 3.66 -1.33 -13.88 1.27 -1.01 5.76 -1.32
Cell Periphery 2.52 3.06 2.66 0.38 -1.06 -4.27 0.70 -1.63 0.96 -1.91 -2.79 2.96 -1.01 0.68 -2.36
Cytoplasm -3.47 1.04 1.47 3.95 0.92 11.85 10.68 12.93 7.28 0.61 10.88 17.40 13.15 8.01 1.28
Cytoplasmic Foci 4.93 25.32 21.91 32.93 -4.07 2.67 29.57 18.47 18.16 -5.22 0.49 40.90 26.76 32.97 -7.69
Eisosomes 2.17 2.87 2.48 4.17 -6.89 -4.77 4.81 -1.65 3.21 -4.94 0.27 4.88 2.06 4.38 -10.23
Endoplasmic Reticulum -4.82 -14.40 -5.44 -4.81 0.64 -3.90 -7.46 -6.17 -3.80 4.16 -4.78 -15.70 -8.16 -6.07 2.91
Endosome -3.33 5.87 4.48 13.95 -3.39 -10.11 5.20 4.20 12.49 -1.44 -13.42 9.17 5.32 19.17 -4.37
Golgi -5.67 6.80 1.57 4.66 -1.00 -4.65 3.75 3.65 10.80 -1.01 -8.56 5.69 2.19 8.81 -1.07
Lipid Particles 2.79 5.00 2.83 1.05 -1.29 -1.53 5.75 6.08 9.24 -0.24 0.49 8.69 4.09 4.39 -1.75
Mitochondria -2.38 3.98 3.27 8.71 -0.82 -7.29 3.08 3.08 11.87 0.12 -8.91 4.71 3.82 14.55 -0.69
Mitotic Spindle -0.33 0.12 -0.94 -0.87 -1.16 -4.55 -1.40 -1.39 1.94 1.06 -4.42 -1.69 -1.89 0.70 -0.24
None -0.68 -0.47 -0.09 0.89 0.61 -1.32 -1.06 -0.53 0.52 0.40 -1.11 0.10 -0.24 0.75 -0.39
Nuclear Periphery -1.98 -20.18 -9.18 -8.38 0.68 -1.83 -5.93 -7.48 -5.11 1.50 -1.88 -17.03 -10.51 -8.67 2.04
Nuclear Periphery Foci -3.46 -7.54 -6.77 -5.48 -1.24 0.06 -5.97 -5.13 -4.95 3.36 -1.25 -8.80 -7.69 -7.02 0.70
Nucleolus -2.28 2.36 3.43 6.26 2.22 -3.81 0.24 2.07 5.61 0.72 -4.77 2.45 3.72 8.12 0.98
Nucleus 2.90 -37.52 -6.65 -7.80 9.84 3.10 -13.50 -10.78 -11.92 3.08 7.91 -39.77 -11.59 -14.41 11.25
Peroxisomes 3.24 6.04 5.98 15.88 -0.97 -6.98 5.55 4.84 13.34 -1.11 -2.42 7.47 7.17 20.69 -1.61
Vacuole -8.38 -6.07 -10.72 -9.54 -9.52 -14.94 -7.77 -10.64 -8.46 -3.27 -18.94 -10.84 -14.81 -12.38 -11.25
Vacuole Periphery -6.83 5.60 5.36 12.39 -1.58 -5.84 3.58 2.45 9.57 -1.35 -8.70 4.72 3.69 15.06 -1.58
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
Localization
Cell Percentages cytoplasm (17%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mrp21

Mrp21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrp21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available