Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0.05 0.1 0.11 0.18 0.11 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.21 0 – 0.13 0.07 0.09 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0
Nucleus 0.82 0.9 0.94 0.91 0.87 0.81 0.87 – 0.82 0.77 0.8 0.74 0.86 0.78 0.71 0.81 0.89 0.78 0.75 0.87 0.79 0.64 0.63 0.56
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.32 0.13 0.13 0.12 0.1 0.27 0.16 – 0.39 0.3 0.31 0.36 0.13 0.18 0.12 0.14 0.15 0.08 0.17 0.06 0.15 0.27 0.24 0.3
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 1 0 0 0 1 2 0
Bud 0 1 0 0 0 1 1 – 0 2 0 0 0 1 0 1 0 2 3 2 0 2 6 3
Bud Neck 0 0 0 1 0 1 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 2 2 1 0 – 1 8 6 5 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 12 3 5 10 7 9 – 1 7 4 14 21 48 68 12 3 6 0 1 1 1 1 2
Endoplasmic Reticulum 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 1 0 0 0 1 2 0
Endosome 1 4 0 1 0 0 2 – 0 0 0 0 0 1 6 0 1 3 7 5 1 1 4 5
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 1 1 5 0 1 1 0 2
Mitochondria 6 31 0 1 1 56 7 – 34 20 22 24 0 20 0 2 1 4 2 4 2 1 3 4
Nucleus 373 501 239 164 250 219 271 – 219 212 195 223 182 333 271 91 84 90 370 537 219 189 273 252
Nuclear Periphery 2 2 0 1 2 2 1 – 3 4 1 0 2 2 3 1 0 0 0 0 0 0 2 1
Nucleolus 144 71 33 22 28 74 51 – 104 83 76 108 28 76 46 16 14 9 84 38 42 81 104 138
Peroxisomes 1 2 0 0 0 0 0 – 0 0 0 0 0 1 7 0 0 1 0 0 0 0 0 0
SpindlePole 1 4 6 0 0 0 4 – 1 0 1 2 2 4 8 1 0 0 3 4 1 1 5 10
Vac/Vac Membrane 5 5 1 1 3 2 2 – 0 1 2 0 1 2 3 2 1 7 10 18 6 11 23 28
Unique Cell Count 455 558 255 181 286 271 311 266 274 243 301 211 426 382 112 94 115 492 617 279 297 433 454
Labelled Cell Count 542 633 282 199 297 363 348 364 337 307 377 237 489 412 128 105 125 492 617 279 297 433 454


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 7.5 8.1 7.8 8.4 5.6 7.2 – 6.4 6.7 6.7 6.7 9.1 7.7 9.3 11.0 11.6 11.8 7.8 8.7 9.0
Std Deviation (1e-4) 2.2 1.6 1.5 1.5 1.7 1.5 1.5 – 1.4 1.5 1.5 1.6 2.5 2.6 2.8 2.8 2.6 2.4 1.9 1.7 1.8
Intensity Change (Log2) – – – -0.04 0.05 -0.53 -0.17 – -0.35 -0.27 -0.26 -0.27 0.17 -0.08 0.2 0.44 0.51 0.54 -0.05 0.1 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.8 0 1.4 – 0 0 0 2.4 4.3 4.8 6.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.7 0 – 5.9 4.4 4.9 4.6 0 3.5 0 0 0 0
Nucleus -1.2 -2.5 -4.4 -2.6 – -4.0 -5.3 -4.5 -6.2 -2.7 -5.4 -7.1 -3.7 -1.4 -4.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.2 -1.2 4.1 1.2 – 6.8 4.8 4.9 6.2 0.1 1.7 -0.3 0.3 0.5 -1.4
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 -0.2 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1513 2.2593 1.7698 1.4441 1.3277 2.0219 3.935 3.9355 3.908 3.3634 3.9954 3.8006 7.4738 8.2516 7.3008 6.9649 6.8536 6.9288
Actin 0.0062 0 0.0017 0.0013 0.0001 0 0.0293 0 0.0123 0.0041 0.0217 0.0004 0.001 0 0 0 0.0002 0
Bud 0.001 0.0002 0.0001 0.0002 0.0001 0 0.0005 0 0.0002 0.0004 0.0016 0.0002 0 0 0 0 0.0001 0
Bud Neck 0.0019 0.0001 0.0003 0.0001 0.0005 0.0011 0.0017 0.0001 0.0006 0.0004 0.0025 0.0009 0.0001 0.0001 0.0001 0 0.0009 0.0003
Bud Periphery 0.0034 0.0002 0.0007 0.0003 0.0002 0.0001 0.0014 0 0.0002 0.0011 0.0049 0.0012 0 0 0 0 0.0001 0
Bud Site 0.0041 0.0002 0.0001 0.0011 0.0002 0.0001 0.0018 0 0.0034 0.0008 0.0027 0.0002 0.0001 0 0 0 0.0001 0
Cell Periphery 0.0004 0 0.0004 0 0.0001 0 0.0003 0 0.0001 0.0003 0.0005 0.0001 0 0 0 0 0 0
Cytoplasm 0.0015 0 0.0002 0.0003 0.0001 0 0.0067 0.0001 0.0006 0.0007 0.0015 0.0001 0.0019 0.0001 0 0 0.0001 0
Cytoplasmic Foci 0.0035 0 0.0001 0.0087 0 0 0.01 0 0.0027 0.0008 0.0026 0.0004 0.0004 0 0 0 0 0
Eisosomes 0.0002 0 0.0002 0 0 0 0.0002 0 0.0001 0.0001 0.0004 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0 0.0003 0.0002 0 0 0.0073 0 0.0007 0.0039 0.0033 0.0001 0.0016 0 0 0 0 0
Endosome 0.0104 0 0.0009 0.0153 0.0001 0.0001 0.02 0 0.0032 0.0087 0.0102 0.0018 0.006 0 0 0 0.0001 0
Golgi 0.0039 0 0.0003 0.005 0 0 0.0033 0 0.0021 0.0024 0.0108 0.0009 0.0002 0 0 0 0 0
Lipid Particles 0.0089 0 0.0007 0.0224 0 0 0.0119 0 0.0056 0.0037 0.032 0.0097 0.0002 0 0 0 0 0
Mitochondria 0.0168 0.0002 0.0079 0.0076 0.0006 0.0004 0.0023 0.0001 0.0048 0.03 0.0495 0.007 0.0005 0.0001 0.0001 0.0001 0.0006 0.0001
None 0.0032 0 0.003 0.0008 0.0001 0 0.0026 0 0.0008 0.0004 0.0007 0.0001 0.0011 0 0 0 0.0001 0
Nuclear Periphery 0.015 0.0004 0.0052 0.0019 0.0012 0.001 0.0138 0.0007 0.0027 0.0129 0.0251 0.001 0.0131 0.0007 0.0003 0.0003 0.0023 0.0001
Nucleolus 0.0412 0.0272 0.03 0.0143 0.1756 0.108 0.05 0.0189 0.0199 0.0127 0.2249 0.0688 0.0246 0.0186 0.0199 0.0136 0.1266 0.0602
Nucleus 0.8544 0.9668 0.9452 0.902 0.8186 0.8883 0.8244 0.9793 0.9317 0.9065 0.5659 0.9048 0.9469 0.9799 0.9793 0.9858 0.8607 0.9376
Peroxisomes 0.0036 0 0.0002 0.0117 0 0 0.001 0 0.0049 0.0004 0.0033 0.0005 0 0 0 0 0 0
Punctate Nuclear 0.0118 0.0042 0.0009 0.0057 0.0023 0.0005 0.0085 0.0008 0.0029 0.0055 0.0303 0.0007 0.001 0.0003 0.0003 0.0002 0.0078 0.0015
Vacuole 0.0045 0.0003 0.0009 0.0005 0.0002 0.0001 0.0019 0 0.0004 0.0023 0.003 0.0006 0.0005 0 0 0 0.0001 0
Vacuole Periphery 0.003 0.0001 0.0009 0.0006 0.0001 0.0001 0.0009 0 0.0002 0.0018 0.0026 0.0005 0.0006 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.2708 38.6814 36.4311 40.2206 33.4457 28.2768 42.8714 37.5651 38.9524 42.1167
Translational Efficiency 1.698 1.773 1.5849 1.2335 1.7238 2.496 1.329 1.934 1.6134 1.6313

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
238 121 2506 1 1893 45 230 1555 2131 166 2736 1556

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 804.23 964.36 1038.83 298.07 830.85 951.06 1057.00 1139.72 827.88 960.75 1040.36 1139.18
Standard Deviation 132.50 161.88 170.39 — 134.75 132.70 130.28 174.03 134.76 154.63 167.47 175.28
Intensity Change Log 2 — 0.261964 0.369280 -1.431957 — 0.194948 0.347315 0.456019 — 0.228300 0.358160 -0.185508

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000533 0.001566 0.000501 0.000017 0.000525 0.001135 0.001484 0.000803 0.000526 0.001449 0.000583 0.000802
Bud Neck 0.003037 0.014104 0.022641 0.000001 0.006092 0.007062 0.009701 0.029280 0.005751 0.012195 0.021554 0.029262
Bud Site 0.002066 0.001112 0.000857 0.000006 0.001429 0.002124 0.001633 0.004561 0.001500 0.001387 0.000922 0.004558
Cell Periphery 0.000078 0.000046 0.000035 0.000124 0.000183 0.000069 0.000059 0.000129 0.000171 0.000052 0.000037 0.000129
Cytoplasm 0.000616 0.000179 0.000309 0.000005 0.000881 0.021823 0.000926 0.004613 0.000851 0.006047 0.000361 0.004610
Cytoplasmic Foci 0.000367 0.002378 0.000006 0.000002 0.000264 0.000906 0.000066 0.001689 0.000276 0.001979 0.000011 0.001688
Eisosomes 0.000037 0.000042 0.000045 0.000174 0.000059 0.000048 0.000060 0.000034 0.000057 0.000043 0.000046 0.000034
Endoplasmic Reticulum 0.002239 0.003430 0.001429 0.000000 0.002198 0.005167 0.008054 0.003863 0.002202 0.003901 0.001986 0.003860
Endosome 0.000430 0.000229 0.000044 0.000000 0.000255 0.000593 0.000277 0.002185 0.000274 0.000327 0.000063 0.002183
Golgi 0.000119 0.001140 0.000059 0.000001 0.000190 0.000301 0.000116 0.001652 0.000182 0.000912 0.000064 0.001651
Lipid Particles 0.000365 0.000725 0.000011 0.000002 0.000258 0.001623 0.000318 0.000304 0.000270 0.000968 0.000037 0.000304
Mitochondria 0.000923 0.001699 0.001037 0.000006 0.000952 0.002556 0.001229 0.002182 0.000948 0.001931 0.001053 0.002180
Mitotic Spindle 0.000090 0.003771 0.000470 0.000043 0.002022 0.014843 0.000432 0.015775 0.001807 0.006772 0.000467 0.015765
None 0.003060 0.001147 0.000971 0.999530* 0.004333 0.003861 0.001327 0.003023 0.004190 0.001882 0.001001 0.003663
Nuclear Periphery 0.000095 0.000154 0.000140 0.000000 0.000161 0.004903 0.000443 0.001327 0.000153 0.001441 0.000165 0.001327
Nuclear Periphery Foci 0.000483 0.000090 0.000022 0.000000 0.000363 0.006597 0.000177 0.001115 0.000376 0.001854 0.000035 0.001115
Nucleolus 0.086172 0.106515 0.031405 0.000000 0.100560 0.052436 0.027344 0.008264 0.098953 0.091855 0.031064 0.008258
Nucleus 0.895803* 0.859292* 0.939409* 0.000001 0.877782* 0.864349* 0.941780* 0.911944* 0.879795* 0.860663* 0.939609* 0.911358*
Peroxisomes 0.001369 0.001829 0.000284 0.000089 0.000896 0.001656 0.000979 0.000270 0.000949 0.001782 0.000342 0.000270
Vacuole 0.001398 0.000310 0.000203 0.000000 0.000338 0.004901 0.003447 0.004486 0.000456 0.001554 0.000475 0.004484
Vacuole Periphery 0.000721 0.000244 0.000122 0.000000 0.000261 0.003048 0.000149 0.002500 0.000312 0.001004 0.000124 0.002498

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN -2.17 -8.52 -5.76 1.18 5.53 -2.78 -2.69 -6.15 1.48 -5.16
Bud Neck NaN NaN NaN NaN NaN -0.55 -6.53 -17.06 -9.13 -10.98 -4.02 -21.65 -17.33 -8.95 -5.83
Bud Site NaN NaN NaN NaN NaN -1.17 -2.10 -4.97 -2.19 -3.47 -0.13 0.66 -4.87 -5.35 -6.70
Cell Periphery NaN NaN NaN NaN NaN 2.22 2.67 1.33 -1.99 -4.51 3.07 3.71 1.16 -4.94 -8.60
Cytoplasm NaN NaN NaN NaN NaN -1.23 -1.87 -5.94 0.95 -4.65 -1.11 1.80 -6.00 0.09 -6.58
Cytoplasmic Foci NaN NaN NaN NaN NaN -1.61 2.15 -2.30 -0.06 -3.11 -0.99 3.07 -2.28 0.56 -3.29
Eisosomes NaN NaN NaN NaN NaN 0.11 -1.15 5.91 1.99 6.25 2.13 2.73 5.69 1.94 5.62
Endoplasmic Reticulum NaN NaN NaN NaN NaN -3.18 -9.75 -10.40 1.25 6.25 -3.93 -0.31 -10.48 -0.97 -10.59
Endosome NaN NaN NaN NaN NaN -2.31 -1.95 -3.39 -1.52 -2.92 -0.78 2.99 -3.33 -2.91 -4.02
Golgi NaN NaN NaN NaN NaN -1.01 0.75 -1.48 -1.16 -1.61 -0.99 1.71 -1.50 -0.03 -1.74
Lipid Particles NaN NaN NaN NaN NaN -1.17 -0.50 0.15 1.19 1.02 -1.25 1.61 0.22 1.32 -2.09
Mitochondria NaN NaN NaN NaN NaN -1.39 -2.09 -5.96 -0.10 -5.22 -2.28 -1.27 -6.44 -1.64 -9.09
Mitotic Spindle NaN NaN NaN NaN NaN -1.43 0.83 -4.78 0.80 -5.32 -1.58 1.37 -4.94 -0.51 -5.68
None NaN NaN NaN NaN NaN 0.32 3.89 1.72 0.33 -4.13 2.86 5.02 0.53 -2.06 -3.66
Nuclear Periphery NaN NaN NaN NaN NaN -1.16 -7.13 -7.80 0.82 -4.08 -1.17 -2.91 -7.86 -0.09 -7.47
Nuclear Periphery Foci NaN NaN NaN NaN NaN -1.29 0.79 -1.20 1.22 -1.90 -1.10 2.62 -1.17 0.85 -2.89
Nucleolus NaN NaN NaN NaN NaN 6.01 16.96 31.06* 4.95 3.71 0.84 21.19 32.80* 8.86 16.87
Nucleus NaN NaN NaN NaN NaN 0.75 -10.11 -6.11 -1.75 4.10 1.63 -14.56 -5.74 -3.72 4.93
Peroxisomes NaN NaN NaN NaN NaN -0.95 0.52 3.98 1.47 4.37 -2.02 3.82 4.74 3.71 4.06
Vacuole NaN NaN NaN NaN NaN -2.86 -5.17 -7.44 0.17 0.33 -2.35 -1.57 -7.17 -4.19 -6.86
Vacuole Periphery NaN NaN NaN NaN NaN -1.33 1.36 -2.49 0.67 -2.72 -1.19 2.08 -2.37 -0.75 -2.80
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rox3

Rox3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rox3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available