Standard name | |
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Human Ortholog | |
Description | Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | 5 | 5 | 4 | 7 | 11 | 6 | 14 | 12 | 15 | 9 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 3 | 3 | 8 |
Bud Neck | 0 | 0 | 3 | 5 | 5 | 4 | 5 | 6 | 2 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 5 |
Bud Site | 2 | 0 | 2 | 6 | 4 | 9 | 15 | 6 | 17 | 13 | 13 | 16 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 7 | 12 | 15 | 15 | 17 | 14 | 20 | 10 | 19 | 21 | 14 | 11 | 37 | 45 | 8 | 9 | 11 | 0 | 0 | 1 | 0 | 2 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 6 | 0 | 0 | 0 | 0 | 3 | 3 |
Endosome | 6 | 10 | 13 | 16 | 14 | 11 | 12 | 10 | 7 | 3 | 0 | 23 | 4 | 17 | 28 | 13 | 8 | 12 | 8 | 17 | 40 | 30 | 60 | 75 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Mitochondria | 8 | 37 | 8 | 35 | 25 | 93 | 126 | 69 | 222 | 226 | 203 | 151 | 35 | 10 | 9 | 10 | 3 | 10 | 1 | 3 | 6 | 2 | 12 | 7 |
Nucleus | 104 | 125 | 269 | 216 | 196 | 231 | 280 | 263 | 258 | 207 | 159 | 336 | 178 | 177 | 182 | 108 | 35 | 61 | 83 | 100 | 180 | 21 | 35 | 48 |
Nuclear Periphery | 3 | 1 | 6 | 7 | 4 | 12 | 5 | 3 | 7 | 7 | 3 | 2 | 2 | 5 | 7 | 7 | 6 | 9 | 4 | 2 | 9 | 2 | 6 | 10 |
Nucleolus | 44 | 56 | 56 | 96 | 93 | 86 | 97 | 90 | 106 | 69 | 67 | 121 | 60 | 96 | 141 | 77 | 32 | 46 | 37 | 60 | 106 | 42 | 36 | 36 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
SpindlePole | 9 | 12 | 68 | 78 | 85 | 51 | 55 | 67 | 29 | 26 | 9 | 51 | 4 | 8 | 13 | 20 | 5 | 14 | 21 | 23 | 69 | 47 | 60 | 68 |
Vac/Vac Membrane | 3 | 4 | 6 | 1 | 12 | 12 | 11 | 12 | 16 | 9 | 7 | 29 | 2 | 4 | 9 | 14 | 5 | 5 | 4 | 11 | 31 | 18 | 37 | 45 |
Unique Cell Count | 150 | 207 | 394 | 372 | 372 | 413 | 470 | 459 | 481 | 410 | 362 | 591 | 259 | 321 | 376 | 214 | 85 | 131 | 168 | 226 | 466 | 174 | 272 | 339 |
Labelled Cell Count | 184 | 252 | 448 | 481 | 459 | 533 | 632 | 553 | 688 | 595 | 502 | 753 | 299 | 357 | 438 | 258 | 106 | 174 | 168 | 226 | 466 | 174 | 272 | 339 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 5.5 | 6.1 | 5.6 | 6.0 | 5.0 | 5.1 | 5.2 | 4.5 | 4.3 | 4.0 | 5.0 | 5.4 | 5.8 | 5.6 | 8.5 | 9.2 | 8.3 | 5.5 | 5.4 | 5.3 |
Std Deviation (1e-4) | 1.1 | 0.9 | 0.9 | 1.1 | 1.2 | 1.0 | 1.1 | 1.0 | 1.1 | 1.0 | 0.9 | 1.0 | 1.0 | 1.6 | 1.3 | 1.8 | 2.3 | 2.0 | 1.6 | 1.7 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.03 | -0.31 | -0.26 | -0.23 | -0.44 | -0.52 | -0.62 | -0.3 | -0.19 | -0.08 | -0.14 | 0.48 | 0.58 | 0.43 | -0.17 | -0.2 | -0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0.5 | 1.2 | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 2.0 | 2.8 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 0.7 | 0.8 | -0.1 | 1.0 | -0.9 | 1.2 | 1.9 | -0.6 | 0.8 | 4.5 | 4.7 | 0.5 | 0 | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.7 | 0.3 | -0.5 | -0.7 | -1.0 | 0 | 0 | 0 | 0.5 | -1.4 | 1.3 | 2.6 | 1.6 | 0 | 2.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.4 | 3.2 | 8.8 | 10.0 | 6.6 | 0 | 0 | 0 | 9.8 | 5.8 | 0.9 | 0.3 | 1.8 | 0 | 0 |
Nucleus | -2.9 | -4.4 | -3.6 | -2.6 | -3.3 | -4.4 | -5.1 | -6.7 | -3.6 | 0.1 | -3.6 | -5.6 | -4.3 | -4.7 | -4.5 |
Nuclear Periphery | 0.4 | 0 | 1.3 | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 0 | 0 | 0 |
Nucleolus | 4.0 | 3.8 | 2.5 | 2.5 | 2.1 | 0 | 0 | 0 | 2.5 | 2.9 | 5.1 | 7.4 | 6.2 | 5.1 | 5.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.3 | 1.9 | -2.0 | -2.3 | -1.1 | 0 | 0 | 0 | -4.1 | -6.3 | -6.4 | -6.2 | -2.6 | -2.6 | -1.8 |
Vacuole | 0 | 1.6 | 1.3 | 0.9 | 1.1 | 1.7 | 0.7 | 0.4 | 2.8 | 0 | 0 | 0.9 | 3.3 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7762 | 0.6399 | 0.6372 | 1.0216 | 0.3117 | 0.6334 | 1.5838 | 1.1754 | 1.315 | 1.6791 | 1.3164 | 1.3053 | 1.3387 | 1.0008 | 1.0546 | 1.7847 | 0.876 | 0.8336 |
Actin | 0.0026 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0216 | 0.0001 | 0.0119 | 0.0461 | 0.0266 | 0.0009 | 0.0112 | 0.0073 | 0.0011 | 0.0462 | 0.0087 | 0.008 |
Bud | 0 | 0.0003 | 0.0017 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 |
Bud Neck | 0.0011 | 0.0006 | 0.0006 | 0.0007 | 0.0038 | 0.0018 | 0.0052 | 0.0007 | 0.002 | 0.0098 | 0.0051 | 0.0021 | 0.0035 | 0.0006 | 0.0016 | 0.0045 | 0.002 | 0.0016 |
Bud Periphery | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0003 | 0.0005 | 0.002 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0011 | 0.0001 | 0.0002 |
Bud Site | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0049 | 0.0005 | 0.0018 | 0.0032 | 0.0008 | 0.0003 | 0.0018 | 0.0012 | 0.0008 | 0.0019 | 0.0003 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.0035 | 0.0042 | 0.0016 | 0.0002 | 0.0003 | 0.0002 | 0.0021 | 0.0056 | 0.0007 | 0.0002 | 0.0044 | 0.0002 | 0.0065 | 0.0031 | 0.0008 | 0.0006 | 0.0013 | 0.0003 |
Cytoplasmic Foci | 0.0009 | 0.0003 | 0.0007 | 0.0002 | 0.0017 | 0.0001 | 0.0096 | 0.0003 | 0.003 | 0.003 | 0.0024 | 0.0003 | 0.006 | 0.0004 | 0.003 | 0.0098 | 0.0058 | 0.0005 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0006 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.002 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0.0016 | 0.0002 | 0.0005 | 0.0002 | 0.0026 | 0.0005 | 0.0021 | 0.0004 | 0.0002 | 0.0007 | 0.0022 | 0.0002 |
Endosome | 0.0021 | 0.0007 | 0.0027 | 0.0001 | 0.0006 | 0.0002 | 0.009 | 0.0003 | 0.004 | 0.0029 | 0.0128 | 0.0031 | 0.016 | 0.0006 | 0.0021 | 0.0198 | 0.016 | 0.0014 |
Golgi | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0042 | 0.0001 | 0.0016 | 0.0051 | 0.006 | 0.0013 | 0.0032 | 0.0005 | 0.0007 | 0.0155 | 0.0028 | 0.0012 |
Lipid Particles | 0.001 | 0.0002 | 0.0057 | 0.0003 | 0.0011 | 0.0003 | 0.0136 | 0.0006 | 0.0049 | 0.007 | 0.0049 | 0.0017 | 0.0109 | 0.0003 | 0.0076 | 0.0126 | 0.0106 | 0.0035 |
Mitochondria | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0035 | 0.0004 | 0.0009 | 0.0025 | 0.0462 | 0.0082 | 0.0027 | 0.0006 | 0.0009 | 0.0074 | 0.0015 | 0.0035 |
None | 0.0042 | 0.0038 | 0.0019 | 0.0002 | 0.0003 | 0.0002 | 0.001 | 0.0015 | 0.0006 | 0.0003 | 0.0021 | 0.0002 | 0.0057 | 0.0098 | 0.0016 | 0.0005 | 0.0049 | 0.0004 |
Nuclear Periphery | 0.0337 | 0.0154 | 0.0314 | 0.0006 | 0.0026 | 0.013 | 0.0422 | 0.0293 | 0.0346 | 0.0018 | 0.0112 | 0.0105 | 0.0429 | 0.0281 | 0.022 | 0.0059 | 0.0246 | 0.0158 |
Nucleolus | 0.1149 | 0.1418 | 0.3102 | 0.3514 | 0.3945 | 0.217 | 0.1363 | 0.2111 | 0.3223 | 0.4546 | 0.4271 | 0.216 | 0.1585 | 0.1584 | 0.2892 | 0.403 | 0.1936 | 0.2076 |
Nucleus | 0.7488 | 0.7183 | 0.5498 | 0.3801 | 0.1461 | 0.6585 | 0.6465 | 0.625 | 0.499 | 0.2199 | 0.1765 | 0.6625 | 0.6057 | 0.6354 | 0.4652 | 0.222 | 0.2201 | 0.6359 |
Peroxisomes | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0066 | 0 | 0.001 | 0.0021 | 0.0009 | 0.0002 | 0.0048 | 0.0002 | 0.003 | 0.0069 | 0.0016 | 0.0006 |
Punctate Nuclear | 0.0836 | 0.1117 | 0.091 | 0.2654 | 0.4475 | 0.108 | 0.0866 | 0.1232 | 0.1083 | 0.2391 | 0.2597 | 0.0889 | 0.1138 | 0.1518 | 0.1986 | 0.2359 | 0.5016 | 0.1183 |
Vacuole | 0.0003 | 0.0008 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0019 | 0.0004 | 0.0016 | 0.0004 | 0.003 | 0.0011 | 0.0019 | 0.0005 | 0.0006 | 0.0032 | 0.0006 | 0.0003 |
Vacuole Periphery | 0.0003 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0014 | 0.0006 | 0.0007 | 0.0002 | 0.0045 | 0.001 | 0.0015 | 0.0005 | 0.0004 | 0.0012 | 0.0014 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.628 | 6.9678 | 8.0787 | 8.0968 | 7.5794 | 4.8688 | 11.6685 | 11.5903 | 8.4545 | 9.9972 |
Translational Efficiency | 3.0411 | 2.2678 | 1.1362 | 1.0239 | 1.1133 | 2.3164 | 1.164 | 0.9031 | 1.0948 | 0.9578 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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