Standard name
Human Ortholog
Description Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.12 0.12 0 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.09 0.09 0.05 0.08 0.09 0.18 0.22 0.22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.18 0 0.09 0.07 0.23 0.27 0.15 0.46 0.55 0.56 0.26 0.14 0 0 0.05 0 0.08 0 0 0 0 0 0
Nucleus 0.69 0.6 0.68 0.58 0.53 0.56 0.6 0.57 0.54 0.5 0.44 0.57 0.69 0.55 0.48 0.5 0.41 0.47 0.5 0.44 0.39 0.12 0.13 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Nucleolus 0.29 0.27 0.14 0.26 0.25 0.21 0.21 0.2 0.22 0.17 0.19 0.2 0.23 0.3 0.38 0.36 0.38 0.35 0.22 0.27 0.23 0.24 0.13 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0.06 0.17 0.21 0.23 0.12 0.12 0.15 0.06 0.06 0 0.09 0 0 0 0.09 0.06 0.11 0.13 0.11 0.15 0.27 0.22 0.2
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0.06 0 0 0.05 0.07 0.11 0.14 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud 0 0 5 5 4 7 11 6 14 12 15 9 1 0 0 0 2 0 0 0 4 3 3 8
Bud Neck 0 0 3 5 5 4 5 6 2 4 3 1 0 0 0 0 0 0 0 0 3 1 2 5
Bud Site 2 0 2 6 4 9 15 6 17 13 13 16 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 7 12 15 15 17 14 20 10 19 21 14 11 37 45 8 9 11 0 0 1 0 2 3
Endoplasmic Reticulum 0 0 0 0 1 0 1 0 0 0 0 0 0 1 3 0 1 6 0 0 0 0 3 3
Endosome 6 10 13 16 14 11 12 10 7 3 0 23 4 17 28 13 8 12 8 17 40 30 60 75
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 3
Mitochondria 8 37 8 35 25 93 126 69 222 226 203 151 35 10 9 10 3 10 1 3 6 2 12 7
Nucleus 104 125 269 216 196 231 280 263 258 207 159 336 178 177 182 108 35 61 83 100 180 21 35 48
Nuclear Periphery 3 1 6 7 4 12 5 3 7 7 3 2 2 5 7 7 6 9 4 2 9 2 6 10
Nucleolus 44 56 56 96 93 86 97 90 106 69 67 121 60 96 141 77 32 46 37 60 106 42 36 36
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 1 0 0 0 0 1 0 1 2
SpindlePole 9 12 68 78 85 51 55 67 29 26 9 51 4 8 13 20 5 14 21 23 69 47 60 68
Vac/Vac Membrane 3 4 6 1 12 12 11 12 16 9 7 29 2 4 9 14 5 5 4 11 31 18 37 45
Unique Cell Count 150 207 394 372 372 413 470 459 481 410 362 591 259 321 376 214 85 131 168 226 466 174 272 339
Labelled Cell Count 184 252 448 481 459 533 632 553 688 595 502 753 299 357 438 258 106 174 168 226 466 174 272 339


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 5.5 6.1 5.6 6.0 5.0 5.1 5.2 4.5 4.3 4.0 5.0 5.4 5.8 5.6 8.5 9.2 8.3 5.5 5.4 5.3
Std Deviation (1e-4) 1.1 0.9 0.9 1.1 1.2 1.0 1.1 1.0 1.1 1.0 0.9 1.0 1.0 1.6 1.3 1.8 2.3 2.0 1.6 1.7 1.3
Intensity Change (Log2) – – – -0.14 -0.03 -0.31 -0.26 -0.23 -0.44 -0.52 -0.62 -0.3 -0.19 -0.08 -0.14 0.48 0.58 0.43 -0.17 -0.2 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.5 1.2 0 0 0 0 0.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.0 2.8 0 0 0 0 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.7 0.8 -0.1 1.0 -0.9 1.2 1.9 -0.6 0.8 4.5 4.7 0.5 0 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.7 0.3 -0.5 -0.7 -1.0 0 0 0 0.5 -1.4 1.3 2.6 1.6 0 2.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.4 3.2 8.8 10.0 6.6 0 0 0 9.8 5.8 0.9 0.3 1.8 0 0
Nucleus -2.9 -4.4 -3.6 -2.6 -3.3 -4.4 -5.1 -6.7 -3.6 0.1 -3.6 -5.6 -4.3 -4.7 -4.5
Nuclear Periphery 0.4 0 1.3 -0.6 0 0 0 0 0 0 0 0.4 0 0 0
Nucleolus 4.0 3.8 2.5 2.5 2.1 0 0 0 2.5 2.9 5.1 7.4 6.2 5.1 5.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.3 1.9 -2.0 -2.3 -1.1 0 0 0 -4.1 -6.3 -6.4 -6.2 -2.6 -2.6 -1.8
Vacuole 0 1.6 1.3 0.9 1.1 1.7 0.7 0.4 2.8 0 0 0.9 3.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7762 0.6399 0.6372 1.0216 0.3117 0.6334 1.5838 1.1754 1.315 1.6791 1.3164 1.3053 1.3387 1.0008 1.0546 1.7847 0.876 0.8336
Actin 0.0026 0.0001 0.0001 0 0.0002 0.0001 0.0216 0.0001 0.0119 0.0461 0.0266 0.0009 0.0112 0.0073 0.0011 0.0462 0.0087 0.008
Bud 0 0.0003 0.0017 0 0 0 0.0004 0 0.0002 0.0003 0.0004 0.0001 0.0003 0.0001 0.0001 0.0007 0.0001 0.0001
Bud Neck 0.0011 0.0006 0.0006 0.0007 0.0038 0.0018 0.0052 0.0007 0.002 0.0098 0.0051 0.0021 0.0035 0.0006 0.0016 0.0045 0.002 0.0016
Bud Periphery 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0013 0.0001 0.0003 0.0005 0.002 0.0006 0.0006 0.0002 0.0002 0.0011 0.0001 0.0002
Bud Site 0.0002 0.0006 0.0003 0.0002 0.0002 0.0001 0.0049 0.0005 0.0018 0.0032 0.0008 0.0003 0.0018 0.0012 0.0008 0.0019 0.0003 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0002 0.0004 0.0001 0.0001 0 0.0001 0.0002 0.0001 0
Cytoplasm 0.0035 0.0042 0.0016 0.0002 0.0003 0.0002 0.0021 0.0056 0.0007 0.0002 0.0044 0.0002 0.0065 0.0031 0.0008 0.0006 0.0013 0.0003
Cytoplasmic Foci 0.0009 0.0003 0.0007 0.0002 0.0017 0.0001 0.0096 0.0003 0.003 0.003 0.0024 0.0003 0.006 0.0004 0.003 0.0098 0.0058 0.0005
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0.0006 0.0003 0 0.0002 0 0.0001 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.002 0.0003 0.0002 0 0 0 0.0016 0.0002 0.0005 0.0002 0.0026 0.0005 0.0021 0.0004 0.0002 0.0007 0.0022 0.0002
Endosome 0.0021 0.0007 0.0027 0.0001 0.0006 0.0002 0.009 0.0003 0.004 0.0029 0.0128 0.0031 0.016 0.0006 0.0021 0.0198 0.016 0.0014
Golgi 0.0003 0 0.0002 0.0001 0.0002 0 0.0042 0.0001 0.0016 0.0051 0.006 0.0013 0.0032 0.0005 0.0007 0.0155 0.0028 0.0012
Lipid Particles 0.001 0.0002 0.0057 0.0003 0.0011 0.0003 0.0136 0.0006 0.0049 0.007 0.0049 0.0017 0.0109 0.0003 0.0076 0.0126 0.0106 0.0035
Mitochondria 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0035 0.0004 0.0009 0.0025 0.0462 0.0082 0.0027 0.0006 0.0009 0.0074 0.0015 0.0035
None 0.0042 0.0038 0.0019 0.0002 0.0003 0.0002 0.001 0.0015 0.0006 0.0003 0.0021 0.0002 0.0057 0.0098 0.0016 0.0005 0.0049 0.0004
Nuclear Periphery 0.0337 0.0154 0.0314 0.0006 0.0026 0.013 0.0422 0.0293 0.0346 0.0018 0.0112 0.0105 0.0429 0.0281 0.022 0.0059 0.0246 0.0158
Nucleolus 0.1149 0.1418 0.3102 0.3514 0.3945 0.217 0.1363 0.2111 0.3223 0.4546 0.4271 0.216 0.1585 0.1584 0.2892 0.403 0.1936 0.2076
Nucleus 0.7488 0.7183 0.5498 0.3801 0.1461 0.6585 0.6465 0.625 0.499 0.2199 0.1765 0.6625 0.6057 0.6354 0.4652 0.222 0.2201 0.6359
Peroxisomes 0.0001 0 0.0001 0 0.0002 0 0.0066 0 0.001 0.0021 0.0009 0.0002 0.0048 0.0002 0.003 0.0069 0.0016 0.0006
Punctate Nuclear 0.0836 0.1117 0.091 0.2654 0.4475 0.108 0.0866 0.1232 0.1083 0.2391 0.2597 0.0889 0.1138 0.1518 0.1986 0.2359 0.5016 0.1183
Vacuole 0.0003 0.0008 0.0009 0.0001 0.0002 0.0002 0.0019 0.0004 0.0016 0.0004 0.003 0.0011 0.0019 0.0005 0.0006 0.0032 0.0006 0.0003
Vacuole Periphery 0.0003 0.0003 0.0006 0 0.0001 0.0002 0.0014 0.0006 0.0007 0.0002 0.0045 0.001 0.0015 0.0005 0.0004 0.0012 0.0014 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.628 6.9678 8.0787 8.0968 7.5794 4.8688 11.6685 11.5903 8.4545 9.9972
Translational Efficiency 3.0411 2.2678 1.1362 1.0239 1.1133 2.3164 1.164 0.9031 1.0948 0.9578

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
Localization
Cell Percentages nucleus (36%), nucleolus (18%), mixed (33%)
Cell Cycle Regulation Yes
nucleolus - MA (0.00012)
nucleus - T/G1 (0.0003)
Subcompartmental Group N/A

Brn1

Brn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Brn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available