Standard name
Human Ortholog
Description Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0.07 0.06 0.06 0 0 0 0 0.09 0.08 0.11 0.19 0.27 0.39 0.15 0.11 0.13 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.99 0.99 0.96 0.93 0.89 0.87 0.88 0.79 0.76 0.71 0.99 0.98 0.98 0.91 0.88 0.95 0.97 0.93 0.93 0.93 0.92 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0.06 0.16 0.22 0.2 0.2 0.35 0.38 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 2 0
Bud 0 0 6 3 2 5 11 5 5 3 5 9 1 0 2 0 0 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 9 14 8 10 8 13 12 12 20 11 19 33 51 71 18 10 14 0 0 0 0 0 0
Cytoplasm 77 281 213 132 167 214 301 261 261 181 110 125 171 185 180 112 82 105 91 291 226 97 129 175
Endoplasmic Reticulum 2 5 0 1 1 2 1 3 2 5 0 3 1 2 1 2 3 2 0 1 2 2 3 3
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0
Mitochondria 0 6 2 12 10 38 74 59 59 80 55 79 0 0 0 3 0 2 0 4 2 0 0 0
Nucleus 0 4 0 1 0 1 3 1 1 2 4 2 1 1 0 4 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 0 1 0 1 4 1 0 1 1 0 0 0 6 1 4 0 5 3 0 0 1
Unique Cell Count 80 294 215 134 174 231 338 299 298 229 145 175 173 189 183 123 93 110 95 313 243 105 140 186
Labelled Cell Count 83 307 235 158 191 269 408 347 343 291 188 240 207 239 254 145 98 130 95 313 243 105 140 186


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 7.8 5.6 5.5 5.5 5.1 4.7 4.7 4.7 4.1 4.2 4.0 8.9 8.9 8.0 13.7 14.2 15.2 7.9 9.4 10.1
Std Deviation (1e-4) 1.2 1.2 0.6 0.9 0.9 0.9 1.1 1.0 1.0 0.9 1.0 0.9 1.2 1.2 1.1 2.6 3.4 2.4 1.4 1.6 1.7
Intensity Change (Log2) – – – -0.02 -0.01 -0.13 -0.25 -0.24 -0.24 -0.45 -0.42 -0.5 0.67 0.68 0.51 1.29 1.35 1.44 0.5 0.75 0.85

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 -0.4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.2 -0.3 -1.5 0 0 0 0 0 0 3.8 5.6 7.8 2.5 1.3 1.9
Cytoplasm -0.5 -2.0 -3.4 -4.5 -4.9 -4.8 -6.7 -7.1 -8.0 -0.2 -1.0 -0.6 -3.7 -4.4 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.7 2.7 5.7 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5264 5.8543 5.102 5.0423 4.1311 5.4014 6.6842 7.7093 7.4455 6.8011 7.4622 7.3904 7.2897 7.8938 7.4702 8.2628 6.6313 7.7576
Actin 0.1053 0.0046 0.0156 0.0586 0.0086 0.0048 0.0558 0.0044 0.0264 0.0426 0.0186 0.0059 0.0231 0.0033 0.0032 0.0044 0.0003 0.0168
Bud 0.0014 0.0003 0.0005 0.0009 0.002 0.0006 0.003 0.0096 0.0027 0.0019 0.0016 0.003 0.0005 0.0002 0.0004 0.0005 0.0002 0.0021
Bud Neck 0.003 0.0006 0.0009 0.0008 0.0039 0.0009 0.0054 0.0004 0.0034 0.0009 0.0012 0.0032 0.0007 0.0004 0.0005 0.0007 0.0006 0.0055
Bud Periphery 0.0023 0.0002 0.0008 0.0017 0.0013 0.0006 0.0043 0.0037 0.0026 0.0038 0.0014 0.0015 0.0008 0.0002 0.0004 0.0004 0.0001 0.002
Bud Site 0.0047 0.0187 0.0097 0.0006 0.0213 0.0006 0.0222 0.0229 0.0196 0.0039 0.0047 0.0012 0.0029 0.0066 0.0025 0.0006 0.0002 0.0005
Cell Periphery 0.0014 0.0024 0.0016 0.0014 0.0007 0.0001 0.0025 0.0021 0.0021 0.0009 0.0005 0.0009 0.0025 0.0019 0.0023 0.0009 0.0001 0.0007
Cytoplasm 0.6342 0.9555 0.934 0.9056 0.7681 0.9342 0.5449 0.9007 0.826 0.7972 0.6705 0.936 0.8029 0.9749 0.9437 0.9682 0.9788 0.9369
Cytoplasmic Foci 0.0453 0.0033 0.0033 0.0034 0.0358 0.0035 0.0255 0.0072 0.0098 0.0121 0.0513 0.0082 0.024 0.0029 0.0043 0.0044 0.0035 0.0065
Eisosomes 0.0041 0 0.0001 0.0003 0.0001 0 0.0005 0.0001 0.0015 0.0003 0.0002 0 0.0005 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0182 0.0054 0.0031 0.0048 0.0028 0.0032 0.0278 0.0073 0.0071 0.0043 0.0096 0.0065 0.009 0.0027 0.0033 0.0037 0.0035 0.0124
Endosome 0.0289 0.0019 0.0041 0.0053 0.0498 0.0187 0.0429 0.0067 0.022 0.035 0.0529 0.0031 0.0184 0.0008 0.0023 0.0029 0.0059 0.006
Golgi 0.0116 0.0009 0.0054 0.0035 0.0168 0.0052 0.0153 0.0018 0.0064 0.0142 0.0214 0.0011 0.0193 0.0006 0.0008 0.0005 0.0003 0.0047
Lipid Particles 0.0199 0.0003 0.0027 0.0003 0.0076 0.0007 0.0074 0.0009 0.0048 0.0023 0.0031 0.0004 0.0034 0.0003 0.0003 0.0003 0 0.0004
Mitochondria 0.0331 0.0001 0.0056 0.0018 0.0141 0.0109 0.0083 0.0005 0.0041 0.0138 0.0064 0.0005 0.0111 0 0.0001 0 0.0002 0.0005
None 0.0162 0.0038 0.007 0.0028 0.0296 0.0015 0.1195 0.0257 0.0351 0.037 0.0798 0.0234 0.0374 0.0037 0.0335 0.0088 0.0027 0.0031
Nuclear Periphery 0.0283 0.0003 0.0007 0.0019 0.0155 0.0018 0.0223 0.0008 0.0031 0.0025 0.0311 0.0005 0.017 0.0002 0.0003 0.0004 0.0007 0.0002
Nucleolus 0.0006 0 0.0002 0 0.0012 0.0001 0.002 0.0002 0.0005 0.0004 0.0003 0.0002 0.0003 0 0 0 0 0
Nucleus 0.0047 0.0004 0.0008 0.0009 0.0038 0.0009 0.0288 0.0013 0.0029 0.0021 0.0112 0.0014 0.0133 0.0004 0.0004 0.0005 0.0007 0.0003
Peroxisomes 0.0232 0 0.0007 0.0001 0.005 0.0008 0.0171 0.0002 0.0036 0.0009 0.0236 0.0002 0.0081 0 0.0001 0 0 0.0007
Punctate Nuclear 0.0073 0.0001 0.0008 0.0002 0.0047 0.0001 0.0287 0.0011 0.0009 0.0004 0.0053 0.0009 0.0014 0.0001 0.0002 0.0002 0.0001 0.0001
Vacuole 0.0027 0.001 0.0017 0.0045 0.0053 0.0058 0.0131 0.0021 0.012 0.0198 0.0031 0.0016 0.0023 0.0007 0.0013 0.0026 0.0015 0.0007
Vacuole Periphery 0.0038 0.0001 0.0006 0.0005 0.0023 0.0049 0.0026 0.0002 0.0033 0.0036 0.0023 0.0002 0.0012 0 0.0001 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.7781 15.5867 17.6764 24.2508 13.2227 19.3992 23.3423 29.803 29.5424 23.6994
Translational Efficiency 0.4009 0.4323 0.5717 0.4497 0.4556 0.6068 0.5423 0.4508 0.5273 0.4513

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2364 1256 114 972 1789 1543 249 705 4153 2799 363 1677

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 857.23 838.35 1334.56 919.87 813.34 886.41 1122.18 902.76 838.32 864.84 1188.88 912.68
Standard Deviation 102.84 136.50 174.09 128.76 109.92 122.38 128.06 112.76 108.15 131.10 174.59 122.58
Intensity Change Log 2 — -0.032130 0.638610 0.101748 — 0.124116 0.464374 0.150484 — 0.046055 0.556405 0.125681

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000453 0.000497 0.000726 0.000745 0.000256 0.000774 0.000415 0.000554 0.000368 0.000650 0.000513 0.000665
Bud Neck 0.005468 0.007993 0.001295 0.004810 0.003453 0.009732 0.001698 0.002356 0.004600 0.008952 0.001571 0.003778
Bud Site 0.031220 0.040874 0.014214 0.028194 0.019974 0.048726 0.018718 0.018717 0.026376 0.045202 0.017303 0.024210
Cell Periphery 0.000690 0.000172 0.000245 0.000117 0.000240 0.000206 0.000223 0.000076 0.000496 0.000191 0.000230 0.000099
Cytoplasm 0.756591 0.628815 0.807558 0.551988 0.749693 0.617957 0.492000 0.540813 0.753619 0.622829 0.591101 0.547290
Cytoplasmic Foci 0.125152 0.186845 0.060668 0.047011 0.129188 0.193039 0.075007 0.071545 0.126890 0.190259 0.070504 0.057325
Eisosomes 0.000187 0.000068 0.000014 0.000041 0.000047 0.000117 0.000066 0.000037 0.000127 0.000095 0.000050 0.000040
Endoplasmic Reticulum 0.001308 0.000816 0.000932 0.000916 0.001910 0.001221 0.001160 0.000527 0.001567 0.001040 0.001088 0.000752
Endosome 0.005518 0.026486 0.006713 0.012528 0.009911 0.025463 0.009984 0.016447 0.007410 0.025922 0.008957 0.014176
Golgi 0.014957 0.008851 0.001086 0.004829 0.009529 0.011994 0.000201 0.004421 0.012619 0.010584 0.000479 0.004657
Lipid Particles 0.001827 0.002131 0.001742 0.001799 0.001638 0.003008 0.001815 0.000965 0.001746 0.002614 0.001792 0.001448
Mitochondria 0.001620 0.001676 0.000009 0.004085 0.000769 0.002716 0.000054 0.005091 0.001254 0.002249 0.000040 0.004508
Mitotic Spindle 0.000951 0.001841 0.000904 0.041735 0.000702 0.006698 0.002184 0.008252 0.000844 0.004519 0.001782 0.027659
None 0.005717 0.001246 0.000592 0.001822 0.003713 0.000367 0.000413 0.003566 0.004854 0.000761 0.000469 0.002555
Nuclear Periphery 0.000019 0.000143 0.000081 0.000498 0.000042 0.000068 0.000351 0.000277 0.000029 0.000102 0.000266 0.000405
Nuclear Periphery Foci 0.000425 0.000859 0.018949 0.002085 0.000599 0.000641 0.009640 0.001875 0.000500 0.000739 0.012563 0.001996
Nucleolus 0.000263 0.000712 0.000039 0.000356 0.000277 0.000172 0.000102 0.000067 0.000269 0.000414 0.000082 0.000235
Nucleus 0.010626 0.008852 0.015500 0.029900 0.010301 0.005568 0.019543 0.013836 0.010486 0.007042 0.018273 0.023147
Peroxisomes 0.001147 0.003474 0.000196 0.003897 0.001127 0.004071 0.000418 0.002688 0.001139 0.003803 0.000348 0.003389
Vacuole 0.033617 0.074244 0.068249 0.261126 0.053616 0.064582 0.365781 0.306199 0.042232 0.068918 0.272341 0.280074
Vacuole Periphery 0.002243 0.003403 0.000288 0.001518 0.003017 0.002881 0.000228 0.001693 0.002576 0.003115 0.000247 0.001591

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.68 -2.71 0.90 1.33 2.94 -4.29 -2.08 -0.74 2.70 0.45 -3.88 -2.56 0.13 3.09 1.94
Bud Neck -4.22 12.09 6.26 8.29 -3.55 -9.54 7.63 5.53 12.06 0.02 -9.55 14.84 8.11 14.07 -3.12
Bud Site -4.04 6.99 7.78 9.60 0.27 -11.45 1.99 3.61 11.99 1.02 -10.64 6.13 8.32 15.28 0.73
Cell Periphery 9.45 6.92 11.36 8.35 6.23 2.25 2.67 16.08 13.07 5.51 9.52 8.17 13.68 14.76 7.62
Cytoplasm 15.60 -1.30 24.69 10.38 12.92 15.96 16.53 23.69 11.90 -1.19 22.74 12.56 34.08 15.48 3.41
Cytoplasmic Foci -11.34 10.92 28.71 29.67 4.63 -11.31 11.47 18.27 27.35 2.32 -16.30 15.57 33.11 41.04 5.33
Eisosomes 8.55 12.56 11.33 8.13 -6.94 -6.94 -3.37 4.20 8.48 7.17 3.34 9.49 11.78 10.63 4.63
Endoplasmic Reticulum 1.56 1.18 2.59 1.88 1.45 1.23 1.76 3.60 2.28 4.98 1.64 1.90 4.39 2.66 4.66
Endosome -11.89 -0.58 0.20 11.06 0.62 -10.46 2.21 1.71 8.92 0.57 -16.44 -0.13 1.55 14.77 1.41
Golgi 6.70 22.68 17.10 8.23 -1.16 -2.96 21.27 10.32 11.07 -2.17 3.33 30.92 19.84 14.06 -2.90
Lipid Particles -1.05 0.03 2.77 2.98 1.87 -2.17 -0.85 6.22 3.59 5.59 -2.33 -0.28 4.95 4.28 4.32
Mitochondria -0.07 6.12 0.32 0.32 -2.34 -3.17 10.60 -0.95 0.81 -1.66 -2.26 7.99 -0.52 1.00 -2.75
Mitotic Spindle -0.91 0.10 -5.48 -5.07 -5.52 -4.10 -1.26 -2.14 1.14 -1.15 -4.00 -1.15 -5.86 -3.79 -5.19
None 7.45 8.81 6.65 -1.39 -4.15 6.34 6.09 1.87 -1.33 -1.21 10.09 10.85 5.50 -2.06 -2.66
Nuclear Periphery -2.68 -6.32 -9.27 -5.09 -7.32 -1.21 -12.25 -7.21 -5.87 2.35 -3.06 -13.08 -11.52 -7.71 -2.43
Nuclear Periphery Foci -2.80 -7.95 -3.91 -2.40 7.30 -0.32 -11.61 -3.20 -2.58 9.67 -2.02 -12.52 -4.98 -3.49 10.93
Nucleolus -2.32 11.64 0.80 2.45 -4.50 3.40 10.64 12.30 3.47 0.76 -1.71 13.86 3.91 2.72 -3.68
Nucleus 2.05 -3.88 -16.98 -16.45 -9.09 7.35 -7.12 -8.40 -12.29 1.95 6.78 -7.97 -18.58 -20.73 -4.36
Peroxisomes -6.90 15.78 -0.59 3.40 -2.49 -6.96 7.60 0.02 4.15 -1.27 -9.62 14.52 -0.44 5.38 -2.60
Vacuole -13.37 -3.98 -35.53 -29.94 -16.99 -3.76 -20.86 -33.17 -31.94 0.14 -12.98 -18.09 -48.32 -43.64 -4.18
Vacuole Periphery -2.81 16.08 7.07 6.40 -1.31 0.33 19.05 6.79 4.28 -1.39 -1.73 25.57 9.82 7.38 -2.06
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ecm21

Ecm21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available