Standard name
Human Ortholog
Description Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.27 0.23 0.36 0.43 0.5 0.53 0.44 0.45 0.46 0.43 0.44 0.2 0.25 0.28 0.3 0.18 0.28 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 0.1 0 0 0 0 0 0 0 0.06 0.08 0.09 0.11 0.05 0 0 0 0 0 0 0 0 0.06
Endosome 0 0.06 0.12 0.23 0.18 0.08 0.05 0.05 0.07 0.12 0.05 0 0.1 0.09 0.11 0.11 0.12 0.1 0.19 0.23 0.31 0.21 0.22 0.19
Golgi 0.13 0.1 0.1 0.1 0.05 0 0 0 0 0 0 0 0.18 0.18 0.17 0.1 0.09 0.14 0.15 0.12 0.18 0.19 0.21 0.18
Mitochondria 0 0.07 0.2 0.21 0.19 0.37 0.36 0.42 0.44 0.32 0.46 0.5 0 0 0 0 0.07 0 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.77 0.63 0.48 0.28 0.29 0.17 0.17 0.16 0.12 0.16 0.11 0.11 0.52 0.5 0.58 0.64 0.69 0.61 0.63 0.56 0.42 0.44 0.42 0.47
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 5 5 2 0 6 2 2 10 6 5 13 7 3 1 10 13 17 0 2 2 1 0 1
Bud 0 2 1 2 2 8 9 15 22 22 13 17 0 0 0 0 1 0 0 2 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 3 1 5 9 1 8 4 0 0 0 0 2 2 0 0 0 0 0 0
Cytoplasm 80 81 63 79 123 156 239 184 191 192 131 139 26 37 42 79 48 81 1 6 4 1 4 8
Endoplasmic Reticulum 8 21 27 8 11 14 14 9 8 18 17 26 12 16 7 6 12 12 2 2 4 4 5 14
Endosome 6 18 33 50 52 25 23 21 30 52 14 6 13 14 16 30 32 30 73 79 104 33 40 46
Golgi 46 29 26 23 13 3 1 4 4 4 2 6 23 27 25 25 25 40 57 42 59 30 38 44
Mitochondria 13 21 54 46 53 117 163 176 185 135 138 156 4 3 0 10 19 9 6 11 12 9 7 7
Nucleus 1 0 1 0 1 1 3 3 3 0 0 1 1 0 2 0 0 1 0 0 0 1 2 1
Nuclear Periphery 2 2 2 2 0 0 0 2 0 0 1 2 0 0 0 0 4 0 2 0 2 1 0 1
Nucleolus 3 3 1 0 1 1 4 4 0 2 2 2 0 0 0 2 3 2 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 0 2 0 1 6 1 1 1 0 0 1 0 0 0 0 1 0 0 0
Vac/Vac Membrane 278 189 128 62 83 52 74 68 49 66 33 34 67 75 88 169 186 180 245 194 140 69 77 117
Unique Cell Count 361 300 269 222 286 314 448 419 420 420 302 315 129 151 151 263 269 293 391 345 333 158 185 251
Labelled Cell Count 446 371 342 276 341 386 535 493 514 504 366 407 154 175 181 332 345 374 391 345 333 158 185 251


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.5 10.9 8.6 7.8 7.4 6.8 6.3 6.4 6.5 6.3 6.4 6.6 10.7 10.2 10.8 11.2 10.9 12.1 11.7 11.4 12.0
Std Deviation (1e-4) 2.3 2.3 1.7 1.5 1.6 1.5 1.3 1.3 1.4 1.4 1.4 1.4 2.2 1.7 2.4 2.6 2.9 2.7 2.3 2.1 2.5
Intensity Change (Log2) – – – -0.14 -0.23 -0.35 -0.45 -0.43 -0.42 -0.46 -0.42 -0.39 0.31 0.24 0.33 0.37 0.34 0.49 0.44 0.41 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0.5 0 0 1.6 0 0 0 1.4 1.9 2.4
Bud 0 0 0 0 2.7 3.5 3.5 3.0 3.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.0 4.9 6.5 7.9 5.5 5.9 5.9 5.0 5.2 -0.7 0.3 1.0 1.7 -1.6 1.1
Endoplasmic Reticulum -2.8 -2.9 -2.6 -3.9 -4.5 -4.7 -3.0 -2.0 -0.7 -0.2 0.2 -1.9 -3.7 -2.5 -2.8
Endosome 3.0 1.9 -1.7 -3.4 -3.5 -2.3 0 -3.3 -5.0 -0.6 -0.9 -0.5 -0.3 -0.1 -0.8
Golgi 0.3 -2.4 -4.8 -6.4 -5.5 -5.5 -5.5 -5.0 -4.1 2.3 2.4 2.1 -0.1 -0.1 1.5
Mitochondria 0.2 -0.5 4.5 4.6 6.0 6.4 3.5 6.5 7.4 -4.5 -5.2 -5.9 -5.8 -4.4 -6.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.5 -4.5 -8.1 -9.0 -8.9 -10.5 -9.1 -9.7 -9.9 0.8 0.4 2.1 3.9 5.1 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.7272 14.525 13.2093 13.0209 12.6816 12.7695 12.2343 15.9026 13.5484 15.6094 13.1377 14.028 8.2088 11.6296 10.0373 9.9733 9.6249 10.2625
Actin 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0003 0.0013 0.0001 0 0.0001 0.0001 0.0003 0.0002 0.0049 0 0 0.0006
Bud 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0005 0.0008 0 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0.0007 0.0001 0 0.0002
Bud Periphery 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0003 0.0011 0 0 0.0001 0.0002 0.0001 0.0002 0.0002 0 0.0001
Bud Site 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0007 0.0004 0 0 0 0.0001 0.0002 0.0057 0 0 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0 0 0 0 0.0001 0 0.0003 0.0001 0 0
Cytoplasm 0.0146 0.0266 0.0117 0.0025 0.0098 0.0108 0.0055 0.0155 0.008 0.0011 0.0028 0.0045 0.0067 0.0149 0.0082 0.0025 0.0045 0.0051
Cytoplasmic Foci 0.0245 0.0266 0.0206 0.0121 0.0168 0.0253 0.0217 0.0299 0.0259 0.0109 0.0177 0.0212 0.0195 0.0288 0.0292 0.005 0.0098 0.0138
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0049 0.0057 0.0042 0.0014 0.0081 0.0057 0.0027 0.0042 0.0034 0.0006 0.0013 0.003 0.0036 0.0029 0.0027 0.0016 0.0023 0.0037
Endosome 0.6613 0.5953 0.5787 0.5709 0.5911 0.6015 0.6095 0.6 0.5999 0.6008 0.6223 0.5425 0.6197 0.6097 0.6077 0.5052 0.6899 0.6065
Golgi 0.1261 0.1655 0.1438 0.1037 0.1037 0.1897 0.2537 0.2357 0.2681 0.1637 0.2846 0.3388 0.1805 0.181 0.1557 0.0827 0.0603 0.2056
Lipid Particles 0.0013 0.0018 0.0015 0.0006 0.0006 0.0004 0.0022 0.0003 0.0003 0.0001 0.0004 0.0006 0.0018 0.0005 0.0017 0.0002 0.0006 0.0018
Mitochondria 0.0078 0.0038 0.0055 0.0016 0.001 0.0068 0.0127 0.0032 0.0072 0.0005 0.0055 0.0083 0.0148 0.0045 0.0101 0.0047 0.0034 0.0049
None 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0.0007 0 0.0001 0.0003
Nuclear Periphery 0.0034 0.0056 0.0033 0.0026 0.0034 0.0029 0.0023 0.0019 0.0012 0.0007 0.0006 0.0014 0.0035 0.0022 0.0025 0.003 0.0023 0.0044
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0.0007 0 0 0.0002
Nucleus 0.0002 0.0003 0.0003 0.0002 0.0002 0.0002 0.0002 0.0002 0.0001 0 0 0.0001 0.0004 0.0002 0.0032 0.0002 0.0001 0.0003
Peroxisomes 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.0003 0 0.0001 0 0.0003 0.0002 0.0005 0 0 0.0003
Punctate Nuclear 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0.0011 0 0 0.0008
Vacuole 0.0701 0.088 0.1107 0.126 0.1098 0.0614 0.0377 0.0423 0.0338 0.0665 0.0183 0.0227 0.0701 0.0621 0.0757 0.1566 0.0872 0.0505
Vacuole Periphery 0.085 0.0798 0.1189 0.1781 0.1548 0.0945 0.0508 0.0634 0.0491 0.1551 0.0461 0.0566 0.0778 0.092 0.0883 0.2376 0.1394 0.1008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.1114 85.2424 65.5126 68.6116 68.4398 64.67 75.0302 85.699 88.3839 74.505
Translational Efficiency 0.9341 0.7047 1.0134 0.8669 0.9483 1.3202 0.914 1.0064 0.9849 0.7921

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
6 17 2083 6 1734 23 1422 1447 1740 40 3505 1453

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 996.77 392.61 1245.56 1049.82 894.34 307.04 1151.97 972.01 894.69 343.41 1207.59 972.33
Standard Deviation 87.49 253.99 205.33 85.91 131.88 5.12 161.14 127.95 131.89 170.94 194.17 127.90
Intensity Change Log 2 — -1.344164 0.321462 0.074809 — -1.542397 0.365208 0.120148 — -1.434528 0.342316 0.096428

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009917 0.000057 0.000145 0.000070 0.000088 0.000058 0.000950 0.000651 0.000121 0.000057 0.000471 0.000649
Bud Neck 0.001992 0.000032 0.000257 0.001208 0.000241 0.000027 0.000124 0.006055 0.000247 0.000029 0.000203 0.006035
Bud Site 0.038064 0.000103 0.003900 0.039059 0.004689 0.000051 0.003260 0.017323 0.004804 0.000073 0.003640 0.017413
Cell Periphery 0.000078 0.000120 0.000131 0.000063 0.000136 0.000088 0.000119 0.000030 0.000135 0.000102 0.000126 0.000030
Cytoplasm 0.033196 0.000114 0.005439 0.039222 0.007001 0.000083 0.002156 0.075649 0.007091 0.000096 0.004107 0.075499
Cytoplasmic Foci 0.059239 0.001588 0.041861 0.209068* 0.164690 0.000030 0.072821 0.279401* 0.164326 0.000692 0.054421 0.279111*
Eisosomes 0.000021 0.000079 0.000007 0.000019 0.000018 0.000064 0.000012 0.000025 0.000018 0.000070 0.000009 0.000025
Endoplasmic Reticulum 0.000137 0.000008 0.001061 0.000604 0.000995 0.000001 0.000983 0.000428 0.000992 0.000004 0.001029 0.000429
Endosome 0.233673* 0.004751 0.071221 0.197042 0.137623 0.000003 0.094748 0.218651* 0.137954 0.002021 0.080766 0.218561*
Golgi 0.077907 0.040217 0.488738* 0.224509* 0.378813* 0.000008 0.685037* 0.051986 0.377776* 0.017097 0.568378* 0.052699
Lipid Particles 0.000178 0.000081 0.000842 0.001121 0.000715 0.000056 0.001303 0.002614 0.000713 0.000067 0.001029 0.002608
Mitochondria 0.002662 0.000023 0.004050 0.000857 0.022051 0.000013 0.001958 0.003264 0.021984 0.000017 0.003201 0.003254
Mitotic Spindle 0.000406 0.000437 0.005644 0.004008 0.001251 0.000478 0.000591 0.017425 0.001248 0.000461 0.003594 0.017370
None 0.000020 0.939996* 0.000104 0.000016 0.000154 0.998672* 0.000026 0.000104 0.000153 0.973735* 0.000072 0.000104
Nuclear Periphery 0.000012 0.000006 0.000551 0.000109 0.000055 0.000001 0.000074 0.000268 0.000055 0.000003 0.000358 0.000268
Nuclear Periphery Foci 0.000026 0.000083 0.001844 0.003046 0.000297 0.000131 0.000657 0.000459 0.000296 0.000110 0.001363 0.000470
Nucleolus 0.000009 0.000047 0.000033 0.000050 0.000066 0.000087 0.000005 0.000247 0.000065 0.000070 0.000022 0.000246
Nucleus 0.000026 0.000022 0.000085 0.001323 0.000116 0.000018 0.000013 0.002405 0.000116 0.000020 0.000056 0.002401
Peroxisomes 0.246340* 0.000124 0.000101 0.001307 0.000401 0.000124 0.000125 0.012749 0.001249 0.000124 0.000111 0.012702
Vacuole 0.087377 0.006633 0.172631 0.214898* 0.069518 0.000004 0.061835 0.293059* 0.069580 0.002821 0.127681 0.292736*
Vacuole Periphery 0.208719* 0.005480 0.201354* 0.062403 0.211084* 0.000000 0.073202 0.017204 0.211076* 0.002329 0.149362* 0.017391

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN 0.90 -1.32 -4.96 -5.21 0.48 1.63 -1.31 -4.51 -5.27 -0.54
Bud Neck NaN NaN NaN NaN NaN 5.73 2.48 -13.37 -13.85 -13.62 5.97 0.88 -13.37 -13.86 -13.46
Bud Site NaN NaN NaN NaN NaN 12.77 1.92 -9.23 -13.34 -9.61 12.79 2.03 -9.20 -13.39 -10.01
Cell Periphery NaN NaN NaN NaN NaN 1.27 0.70 5.73 1.78 6.66 0.94 0.33 5.73 2.33 7.61
Cytoplasm NaN NaN NaN NaN NaN 9.57 5.15 -20.68 -22.70 -21.95 9.66 3.02 -20.68 -22.73 -21.48
Cytoplasmic Foci NaN NaN NaN NaN NaN 49.51* 23.57 -15.03 -49.70 -33.16* 48.35 31.11 -15.10 -49.29 -38.23*
Eisosomes NaN NaN NaN NaN NaN -2.52 2.33 -2.07 2.14 -3.91 -3.17 4.68 -2.07 2.74 -5.78
Endoplasmic Reticulum NaN NaN NaN NaN NaN 9.79 0.06 5.02 -8.70 3.72 9.76 -0.34 5.01 -8.67 5.03
Endosome NaN NaN NaN NaN NaN 43.12* 10.24 -9.89 -41.18* -18.25 35.86* 15.91 -9.82 -37.52* -22.75
Golgi NaN NaN NaN NaN NaN 56.42* -31.97* 46.40* -16.78 87.60* 19.65 -22.17 46.09* -1.61 87.73*
Lipid Particles NaN NaN NaN NaN NaN 4.42 -1.26 -10.46 -19.68 -3.30 4.36 -1.23 -10.47 -19.71 -6.92
Mitochondria NaN NaN NaN NaN NaN 14.59 13.01 12.26 -7.99 -2.08 14.59 12.18 12.26 -7.99 0.47
Mitotic Spindle NaN NaN NaN NaN NaN 2.28 2.00 -8.71 -9.22 -9.17 2.44 -4.58 -8.72 -9.25 -7.31
None NaN NaN NaN NaN NaN -2922.11* 3.16 0.51 2920.66* -2.34 -38.99* 1.85 0.51 38.99* -1.14
Nuclear Periphery NaN NaN NaN NaN NaN 7.58 -1.67 -11.58 -13.98 -10.83 6.84 -4.07 -11.59 -13.85 -0.31
Nuclear Periphery Foci NaN NaN NaN NaN NaN 1.05 -1.57 -1.60 -5.54 0.46 1.21 -5.44 -1.67 -7.09 6.04
Nucleolus NaN NaN NaN NaN NaN -0.46 2.39 -5.79 -4.97 -8.63 -0.07 1.73 -5.79 -6.17 -8.17
Nucleus NaN NaN NaN NaN NaN 1.93 2.02 -10.37 -10.74 -10.76 1.90 1.10 -10.39 -10.75 -10.64
Peroxisomes NaN NaN NaN NaN NaN 3.19 3.65 -13.59 -13.94 -13.96 1.87 1.89 -10.33 -13.96 -13.98
Vacuole NaN NaN NaN NaN NaN 29.04* 1.97 -35.22 -47.07 -35.13 18.07 -15.73 -35.23 -43.36 -26.75
Vacuole Periphery NaN NaN NaN NaN NaN 37.02* 20.68 33.97* -15.69 16.30* 33.94 9.44 33.98 -5.03 39.47*
Standard name
Human Ortholog
Description Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Sft2

Sft2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sft2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available