Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.96 – 0.93 0.98 0.96 0.94 0.94 0.92 0.9 0.79 0.82 0.79 0.94 1.0 0.99 0.96 0.94 0.96 0.95 0.93 0.85 0.85 0.85 0.83
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.06
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.09 0.07 0 0.2 0.16 0.2 0.3 0.39 0.44 0.43 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 2 1
Bud 0 – 1 0 0 1 1 1 5 2 3 3 0 0 4 0 0 0 0 0 0 0 0 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 – 0 0 2 0 2 3 1 5 1 6 0 2 4 13 11 15 0 0 0 0 0 0
Cytoplasm 104 – 52 83 140 122 196 212 167 105 129 198 17 121 158 337 206 333 104 47 66 41 122 136
Endoplasmic Reticulum 1 – 0 0 0 0 1 4 0 2 1 2 0 0 0 15 12 12 0 0 1 1 3 9
Endosome 0 – 0 0 3 1 0 2 1 0 1 0 0 0 0 4 2 1 0 0 0 0 2 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 1 2
Mitochondria 3 – 5 6 4 26 34 45 56 52 70 108 1 0 6 8 1 6 0 0 2 1 0 3
Nucleus 0 – 0 1 0 0 0 1 1 1 1 0 0 0 0 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 1 0 1 0 1 0 1 2 0 0 0 8 8 10 0 1 4 0 4 3
Unique Cell Count 108 56 85 146 130 209 230 186 133 158 250 18 121 159 350 218 348 110 51 78 49 143 164
Labelled Cell Count 111 58 90 150 150 236 269 232 167 207 319 18 124 173 387 243 379 110 51 78 49 143 164


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 – 4.9 5.1 5.2 4.8 4.6 4.6 4.4 4.1 4.1 3.9 7.1 7.4 6.4 8.0 8.4 9.0 – 7.2 7.7
Std Deviation (1e-4) 1.0 – 0.7 0.9 0.7 1.3 1.0 0.9 1.3 0.8 1.1 1.2 0.9 1.2 0.9 1.4 1.9 1.7 – 1.5 1.4
Intensity Change (Log2) – – – 0.06 0.09 -0.03 -0.08 -0.1 -0.17 -0.25 -0.26 -0.33 0.54 0.6 0.39 0.71 0.78 0.88 – 0.55 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.9 0.3 0.3 -0.2 -0.7 -2.3 -2.0 -2.4 0.2 3.0 2.8 1.2 0.5 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6302 4.0164 3.2122 2.8125 2.7697 3.3364 3.4622 4.8581 4.3327 4.1959 3.9141 4.4496 1.1456 2.318 1.5618 1.7647 1.5337 1.5455
Actin 0.0431 0.0004 0.01 0.0475 0.0415 0.0043 0.0318 0.0001 0.0043 0.0001 0.0004 0.0021 0.0002 0.0003 0.0142 0.0019 0.0146 0.0029
Bud 0.0007 0.0002 0.0029 0.003 0.0078 0.0005 0.001 0.0002 0.0053 0.0016 0.0001 0.0005 0.0001 0.0001 0.0006 0.0003 0.0002 0.0001
Bud Neck 0.0028 0.0002 0.0011 0.0016 0.0029 0.0014 0.0014 0.0001 0.0004 0.0003 0.0014 0.0004 0.0001 0.0001 0.0119 0.0001 0.0002 0.0004
Bud Periphery 0.0007 0.0002 0.0021 0.0034 0.0099 0.0007 0.001 0.0001 0.0032 0.0014 0 0.0006 0 0 0.0004 0.0002 0.0003 0.0001
Bud Site 0.009 0.0011 0.0052 0.0007 0.0121 0.0002 0.0041 0.0005 0.0048 0.0005 0.0002 0.0012 0.0014 0.0007 0.0024 0.0002 0.0003 0.0001
Cell Periphery 0.0007 0.0006 0.0005 0.0003 0.0006 0.0003 0.0003 0.0001 0.0003 0.0001 0 0.0001 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.7332 0.9382 0.8688 0.8141 0.7073 0.9209 0.7143 0.9342 0.8706 0.8734 0.8235 0.9282 0.8483 0.9005 0.7539 0.6557 0.7737 0.8815
Cytoplasmic Foci 0.0364 0.0056 0.02 0.0185 0.0233 0.0089 0.0579 0.0068 0.0106 0.0096 0.0503 0.0077 0.0194 0.0036 0.0302 0.0345 0.0127 0.0081
Eisosomes 0.0006 0 0.0001 0.0002 0.0007 0 0.0006 0 0.0003 0 0 0 0 0 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.017 0.0049 0.0069 0.0042 0.0171 0.0101 0.0102 0.0018 0.0055 0.0022 0.0018 0.0046 0.0059 0.001 0.0078 0.0048 0.0054 0.0032
Endosome 0.0463 0.0089 0.0208 0.0574 0.0341 0.016 0.0613 0.0066 0.0214 0.0473 0.0099 0.0214 0.0112 0.0015 0.0433 0.0541 0.0182 0.0057
Golgi 0.0107 0.0007 0.0034 0.0098 0.0096 0.0016 0.0102 0.0003 0.0086 0.0018 0.003 0.004 0.0009 0.0002 0.0067 0.0027 0.0052 0.002
Lipid Particles 0.0137 0.0002 0.0026 0.0012 0.0141 0.0003 0.0139 0.0001 0.0006 0.0003 0.0005 0.0008 0.0005 0 0.0027 0.0031 0.0168 0.0032
Mitochondria 0.0061 0.0003 0.002 0.0011 0.065 0.0021 0.0264 0.0001 0.0363 0.0032 0.0003 0.0107 0.0002 0 0.0024 0.0007 0.0106 0.0057
None 0.0384 0.031 0.0363 0.0072 0.0297 0.0226 0.0154 0.0456 0.0189 0.0098 0.0178 0.0141 0.1061 0.0903 0.0952 0.2325 0.1149 0.0735
Nuclear Periphery 0.0054 0.0008 0.0028 0.0014 0.005 0.003 0.0096 0.0003 0.0016 0.0042 0.0081 0.0003 0.0007 0.0002 0.0015 0.0013 0.0012 0.0002
Nucleolus 0.0006 0 0.0003 0.0001 0.001 0.0001 0.0008 0 0.0001 0.0002 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0
Nucleus 0.0021 0.0008 0.0016 0.0018 0.0019 0.0009 0.0171 0.0006 0.001 0.0024 0.0572 0.0005 0.0006 0.0003 0.0008 0.0008 0.0005 0.0003
Peroxisomes 0.014 0 0.0023 0.0005 0.0067 0.0001 0.0096 0 0.001 0.0001 0.019 0.0004 0.0001 0 0.0175 0.0011 0.0208 0.0108
Punctate Nuclear 0.008 0.0001 0.0026 0.0004 0.0043 0.0002 0.0034 0.0001 0.0003 0.0001 0.0009 0.0001 0.0002 0.0001 0.0008 0.0007 0.0013 0.0001
Vacuole 0.0089 0.0052 0.0067 0.0225 0.0039 0.0036 0.0081 0.0023 0.0039 0.0292 0.0044 0.0016 0.0032 0.0007 0.0056 0.004 0.002 0.0016
Vacuole Periphery 0.0017 0.0005 0.0011 0.003 0.0016 0.002 0.0017 0.0002 0.0009 0.0122 0.001 0.0006 0.0003 0.0001 0.0011 0.0011 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9739 11.3922 13.3847 13.8663 14.2281 12.383 14.3983 12.7412 15.3871 16.5429
Translational Efficiency 0.6168 0.4703 0.4668 0.4438 0.382 0.5044 0.3922 0.3616 0.3311 0.3965

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
259 143 279 163 395 1080 799 114 654 1223 1078 277

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 713.16 982.00 1210.13 977.09 768.99 836.39 986.33 1008.47 746.88 853.42 1044.25 990.00
Standard Deviation 83.58 136.96 123.39 135.04 100.81 114.02 119.21 125.12 98.24 125.95 155.18 131.96
Intensity Change Log 2 — 0.461497 0.762864 0.454266 — 0.121211 0.359106 0.391131 — 0.294970 0.567489 0.421854

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000218 0.000337 0.000510 0.000379 0.000307 0.000918 0.000177 0.001066 0.000272 0.000850 0.000263 0.000662
Bud Neck 0.011306 0.008429 0.002566 0.003134 0.010153 0.008604 0.002095 0.002359 0.010610 0.008584 0.002217 0.002815
Bud Site 0.019481 0.047808 0.018567 0.052376 0.027727 0.024461 0.012525 0.046670 0.024461 0.027190 0.014089 0.050028
Cell Periphery 0.000239 0.000319 0.000142 0.000048 0.000342 0.000103 0.000064 0.000484 0.000301 0.000128 0.000084 0.000228
Cytoplasm 0.580638* 0.410288* 0.573318* 0.501406* 0.481813* 0.455673* 0.562565* 0.580045* 0.520950* 0.450367* 0.565348* 0.533770*
Cytoplasmic Foci 0.261069* 0.321247* 0.134758 0.085130 0.294924* 0.229846* 0.095562 0.137099 0.281517* 0.240533* 0.105706 0.106518
Eisosomes 0.000096 0.000056 0.000021 0.000024 0.000106 0.000058 0.000020 0.000021 0.000102 0.000058 0.000020 0.000023
Endoplasmic Reticulum 0.001356 0.002130 0.001742 0.000187 0.002096 0.001660 0.000718 0.000408 0.001803 0.001715 0.000983 0.000278
Endosome 0.025676 0.075993 0.022829 0.015965 0.054035 0.063876 0.023715 0.012178 0.042804 0.065293 0.023486 0.014407
Golgi 0.007585 0.010926 0.001298 0.000874 0.017857 0.011264 0.000816 0.046181 0.013789 0.011225 0.000941 0.019520
Lipid Particles 0.004194 0.001955 0.002992 0.002488 0.003982 0.003672 0.002749 0.001148 0.004066 0.003471 0.002812 0.001937
Mitochondria 0.009028 0.004503 0.000159 0.000935 0.006210 0.003468 0.000307 0.000107 0.007326 0.003589 0.000268 0.000594
Mitotic Spindle 0.001628 0.000429 0.006721 0.027870 0.003611 0.006235 0.003334 0.002379 0.002826 0.005556 0.004211 0.017379
None 0.000976 0.000041 0.000467 0.000368 0.000530 0.000350 0.001193 0.000248 0.000707 0.000314 0.001005 0.000319
Nuclear Periphery 0.000167 0.000035 0.000312 0.000361 0.000087 0.000121 0.000163 0.000059 0.000118 0.000111 0.000202 0.000236
Nuclear Periphery Foci 0.000646 0.000326 0.009240 0.000915 0.000290 0.000261 0.002043 0.000881 0.000431 0.000269 0.003906 0.000901
Nucleolus 0.001699 0.000288 0.000135 0.000086 0.001205 0.000299 0.000190 0.000044 0.001400 0.000298 0.000176 0.000069
Nucleus 0.011093 0.008852 0.014446 0.005831 0.004521 0.009742 0.014098 0.004769 0.007124 0.009638 0.014188 0.005394
Peroxisomes 0.001663 0.003546 0.000787 0.001845 0.002616 0.003255 0.000939 0.005264 0.002239 0.003289 0.000899 0.003252
Vacuole 0.051970 0.091427 0.207704* 0.298998* 0.077678 0.167126 0.275740* 0.157540 0.067497 0.158275 0.258132* 0.240781*
Vacuole Periphery 0.009274 0.011066 0.001286 0.000779 0.009909 0.009009 0.000989 0.001049 0.009657 0.009249 0.001066 0.000890

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.13 -2.62 0.18 1.84 3.65 -1.19 1.88 -1.06 0.56 -1.65 -1.28 0.33 -0.72 1.05 -0.95
Bud Neck 1.22 6.13 6.63 3.38 2.01 1.15 7.69 7.89 7.85 0.80 1.78 9.90 10.26 8.48 1.30
Bud Site -3.63 0.31 -0.38 2.51 -0.52 1.06 6.45 -0.25 -0.63 -2.24 -0.99 5.70 -0.17 0.31 -2.27
Cell Periphery -0.58 3.84 9.20 2.10 10.07 5.75 6.89 0.62 -0.99 -1.30 5.58 8.29 2.68 0.06 -0.73
Cytoplasm 6.35 0.77 3.51 -2.15 2.80 1.68 -3.78 -1.45 -2.41 0.62 5.55 -2.08 1.05 -2.45 2.42
Cytoplasmic Foci -2.94 10.19 15.05 13.44 6.10 5.99 20.91 15.01 11.92 0.17 4.57 23.48 22.47 20.13 3.25
Eisosomes 3.31 7.63 7.55 4.18 -0.02 4.74 10.15 10.02 6.85 0.34 5.50 12.76 12.45 7.34 -0.26
Endoplasmic Reticulum -0.73 -0.38 2.42 2.19 3.96 1.11 4.47 5.99 5.96 3.67 0.25 3.06 5.94 6.55 5.73
Endosome -4.94 1.49 6.33 6.90 5.09 -1.96 7.44 10.95 18.40 5.77 -5.80 7.22 12.17 19.74 6.98
Golgi -1.59 7.65 8.65 5.53 4.32 3.27 10.20 0.41 -0.29 -1.42 1.74 11.92 1.95 1.32 -1.33
Lipid Particles 2.45 1.39 3.07 1.47 3.18 0.55 1.93 6.11 7.00 1.62 1.19 2.28 5.60 5.72 2.12
Mitochondria 0.77 2.06 2.03 1.05 -0.68 1.05 2.41 2.49 4.06 1.51 1.54 3.13 3.15 3.86 0.29
Mitotic Spindle 0.85 -1.99 -1.83 -2.14 -0.86 -0.91 -0.56 0.24 1.53 1.13 -1.28 -1.37 -1.46 -0.64 -0.70
None 4.05 2.14 1.46 -4.45 -0.97 0.84 -0.74 0.81 -0.05 1.56 2.10 -0.21 1.18 -1.88 1.13
Nuclear Periphery 1.98 -2.16 -1.09 -4.52 1.22 -0.83 -3.58 -2.35 -1.85 1.30 0.27 -3.50 -2.41 -3.22 0.58
Nuclear Periphery Foci 1.00 -9.23 -0.36 -3.95 9.70 0.47 -8.10 -4.71 -4.97 3.79 1.19 -10.64 -2.69 -7.07 9.91
Nucleolus 1.01 1.11 1.13 0.88 1.72 0.88 0.95 1.04 5.92 6.28 1.31 1.42 1.50 4.97 5.68
Nucleus 0.35 -3.05 2.21 0.13 5.59 -4.06 -9.69 -2.81 0.45 3.98 -1.72 -8.67 -1.20 0.85 6.67
Peroxisomes -3.82 4.95 1.35 4.19 -1.40 -0.92 4.48 0.24 0.69 -1.39 -2.07 5.54 1.06 2.44 -1.75
Vacuole -4.22 -11.13 -14.30 -12.31 -6.27 -11.39 -22.37 -9.26 -5.76 -0.51 -13.50 -25.37 -16.65 -11.17 -4.11
Vacuole Periphery -0.50 3.27 3.60 4.43 3.45 0.67 7.49 7.79 14.23 2.22 0.31 7.14 7.56 14.85 3.84
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Sea4

Sea4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sea4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available