Standard name
Human Ortholog
Description Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.29 0.08 0.29 0.36 0.22 0.2 0.14 0.08 0.07 0.07 0.11 0 0.05 0.13 0.11 0.2 0.18 0.15 0.17 0.07 0.11 0.07 0.11 0.13
Bud 0.2 0 0.25 0.14 0.26 0.22 0.19 0.15 0.15 0.14 0.15 0.14 0.05 0.09 0.1 0.11 0.08 0.06 0.31 0.26 0.31 0.44 0.4 0.42
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.2 0.17 0.34 0.24 0.18
Bud Site 0.13 0.12 0.11 0.21 0.22 0.2 0.11 0.05 0.07 0.07 0.07 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.12 0.12 0 0.17 0.06 0.1 0.11 0.08 0.14 0.11 0.12 0.14 0.17 0.31 0.3 0.12 0.13 0.1 0.05 0 0 0 0 0
Cytoplasm 0.48 0.64 0.45 0.27 0.28 0.29 0.33 0.3 0.36 0.36 0.31 0.38 0.88 0.79 0.76 0.65 0.63 0.57 0.24 0.39 0.23 0.08 0.18 0.17
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.27 0.37 0.14 0.28 0.37 0.4 0.52 0.49 0.48 0.54 0 0 0.07 0.07 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0.06 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.09 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0 0 0.1 0.08 0.06 0.05 0 0 0 0 0 0 0 0 0.06 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0 0.05 0.07 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 128 2 32 56 59 51 47 40 16 22 19 8 9 36 35 25 27 19 85 4 14 10 32 43
Bud 86 0 27 21 70 56 64 75 34 43 27 35 9 25 33 13 12 8 160 16 39 71 121 133
Bud Neck 5 0 0 3 7 6 13 15 1 6 1 2 0 0 0 1 2 1 91 12 22 55 72 57
Bud Site 57 3 12 33 60 51 38 23 16 22 13 11 2 2 2 3 3 4 – – – – – –
Cell Periphery 53 3 1 26 15 25 36 41 32 32 21 37 31 84 96 15 20 12 23 1 2 1 1 8
Cytoplasm 213 16 50 41 77 74 108 150 85 110 56 97 163 213 242 80 96 71 124 24 29 12 55 55
Endoplasmic Reticulum 8 1 1 1 1 3 4 10 5 4 4 2 3 7 2 2 10 3 5 0 0 0 1 3
Endosome 5 0 0 1 8 3 1 10 1 1 0 1 2 0 2 1 6 4 2 1 3 1 4 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 0 1 0 0 0
Mitochondria 3 3 30 57 38 71 122 200 120 149 86 140 1 1 21 9 0 6 4 0 4 2 1 4
Nucleus 6 0 0 2 17 17 11 15 4 9 9 9 0 0 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 2 3 5 3 2 1 10 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4 0 1 0 6 9 13 44 5 12 2 14 1 0 0 2 1 3 0 0 0 0 0 0
Peroxisomes 14 1 3 4 11 9 7 9 4 6 0 1 1 0 2 1 3 5 0 0 0 0 0 0
SpindlePole 25 0 0 3 28 20 19 27 3 5 3 5 0 0 0 5 9 14 4 0 2 3 4 2
Vac/Vac Membrane 7 1 3 1 12 9 13 41 8 11 9 19 1 0 2 7 8 10 0 0 3 1 1 4
Unique Cell Count 441 25 110 154 272 254 332 501 233 304 178 257 185 269 318 123 152 125 515 64 128 162 303 322
Labelled Cell Count 615 30 160 249 409 406 499 705 337 434 251 391 223 368 437 165 200 160 515 64 128 162 303 322


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 7.2 5.0 5.0 5.5 5.1 4.8 4.9 4.4 4.4 4.6 4.2 8.1 7.9 6.7 9.8 10.3 11.2 7.8 7.4 7.7
Std Deviation (1e-4) 1.7 1.3 1.0 1.0 1.2 1.7 1.4 1.2 1.5 1.2 1.3 1.1 1.6 1.5 1.2 1.9 2.0 2.2 1.6 1.7 1.4
Intensity Change (Log2) – – – 0.01 0.14 0.04 -0.06 -0.02 -0.17 -0.17 -0.13 -0.24 0.7 0.66 0.42 0.98 1.05 1.17 0.64 0.57 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 -1.5 -1.9 0 -6.2 0 0 0 0 -5.8 -3.6 -4.5 -1.6 -2.2 -2.6
Bud -2.3 0.2 -0.5 0 -2.4 0 0 0 0 -5.0 -3.9 -3.7 -2.8 -3.7 -3.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2.2 2.5 2.1 0 -2.6 0 0 0 0 -3.8 0 0 -2.6 -3.1 -2.3
Cell Periphery 4.2 0 3.0 0 2.7 0 0 0 0 4.2 6.4 6.3 3.4 3.6 2.9
Cytoplasm -3.2 -3.2 -3.0 -2.5 -3.1 -1.6 -1.7 -2.4 -1.4 7.9 6.5 6.0 3.0 2.8 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 -3.1 0.1 0 2.5 0 0 0 0 -7.2 -8.7 -5.8 -4.1 -6.8 -4.8
Nucleus 0 0 2.8 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.5 3.0 0 0 0 0 0 0 0 0 0 0 0 3.6
Vacuole 0 0 0 0 2.0 0 0 0 1.7 0 0 0 0 0 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2723 4.5553 4.2313 4.0979 3.9769 4.5626 6.0746 6.5938 6.2101 5.5624 5.71 6.566 3.2783 3.4973 3.2403 4.0345 3.2764 3.5323
Actin 0.03 0.0163 0.035 0.0563 0.03 0.0155 0.0789 0.0076 0.0256 0.0434 0.0453 0.0138 0.0386 0.0104 0.0321 0.0229 0.0288 0.0165
Bud 0.0109 0.0196 0.2207 0.1064 0.0816 0.0102 0.0431 0.0319 0.2133 0.1116 0.0432 0.0015 0.0331 0.0463 0.1817 0.0863 0.05 0.0068
Bud Neck 0.105 0.0125 0.009 0.0011 0.0018 0.6872 0.0297 0.0059 0.0042 0.0049 0.1002 0.6738 0.1146 0.0095 0.0028 0.004 0.0386 0.5453
Bud Periphery 0.0209 0.0162 0.2629 0.5163 0.3379 0.0176 0.057 0.02 0.2338 0.2337 0.081 0.0031 0.0715 0.0516 0.2607 0.4044 0.1265 0.0147
Bud Site 0.1671 0.4153 0.126 0.0036 0.0039 0.0086 0.157 0.3521 0.0431 0.0111 0.0162 0.0066 0.1854 0.3746 0.0613 0.052 0.0093 0.0064
Cell Periphery 0.0113 0.0101 0.0075 0.0098 0.0124 0.0019 0.0159 0.0034 0.0023 0.0034 0.0107 0.0022 0.0211 0.002 0.004 0.0071 0.0125 0.0017
Cytoplasm 0.5739 0.4759 0.3083 0.2898 0.5125 0.2385 0.4888 0.5641 0.3972 0.308 0.5748 0.2527 0.4349 0.4798 0.3726 0.4069 0.5842 0.3771
Cytoplasmic Foci 0.0106 0.0092 0.0061 0.0021 0.0013 0.0058 0.0231 0.0042 0.0115 0.0199 0.042 0.0109 0.0147 0.0032 0.0082 0.0013 0.0109 0.0183
Eisosomes 0.0004 0.0001 0.0001 0.0001 0 0 0.0005 0 0.0001 0.0007 0.0001 0.0001 0.0004 0 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.0067 0.003 0.0043 0.0035 0.0057 0.0013 0.0064 0.0014 0.0026 0.0058 0.003 0.0012 0.0078 0.0014 0.0019 0.0047 0.0035 0.0011
Endosome 0.0038 0.0016 0.0008 0.0024 0.0013 0.001 0.0307 0.0014 0.0179 0.0875 0.0461 0.0043 0.012 0.0026 0.0037 0.0013 0.0187 0.0016
Golgi 0.0019 0.0008 0.0007 0.0029 0.0008 0.0009 0.0129 0.0009 0.0181 0.0294 0.0055 0.006 0.0092 0.0006 0.0044 0.0003 0.0136 0.0016
Lipid Particles 0.0012 0.0005 0.0003 0.0001 0.0002 0.0004 0.0081 0.0001 0.0036 0.0184 0.0036 0.0024 0.011 0.0001 0.0014 0.0003 0.0176 0.0005
Mitochondria 0.0003 0.0002 0.0005 0.0012 0.0008 0.0002 0.0141 0.0008 0.0121 0.0928 0.001 0.0068 0.0152 0.0003 0.0236 0.0004 0.0082 0.0003
None 0.0316 0.0124 0.0108 0.0007 0.001 0.0055 0.0048 0.0029 0.0045 0.0034 0.0032 0.0025 0.0067 0.0024 0.0329 0.0028 0.0659 0.0008
Nuclear Periphery 0.0009 0.0004 0.0007 0.0007 0.0014 0.0003 0.0048 0.0004 0.0006 0.0037 0.0058 0.0002 0.0019 0.0006 0.0006 0.0006 0.0008 0.0001
Nucleolus 0.001 0.0003 0.0004 0 0.0001 0.0002 0.0004 0.0001 0.0001 0.0006 0.0003 0.0001 0.0004 0.0003 0.0002 0.0005 0.0002 0.0002
Nucleus 0.0015 0.0017 0.0013 0.0008 0.0014 0.001 0.0031 0.0012 0.0011 0.0024 0.003 0.0004 0.0012 0.0032 0.0014 0.001 0.0009 0.0005
Peroxisomes 0.0013 0.0004 0.0005 0.0001 0.0001 0.0004 0.0103 0.0006 0.0051 0.009 0.0028 0.0102 0.0156 0.0001 0.0041 0.0001 0.0054 0.0056
Punctate Nuclear 0.0015 0.0007 0.0009 0.0001 0.0001 0.0003 0.0033 0.0005 0.0003 0.0011 0.0088 0.0004 0.0019 0.0002 0.0004 0.0005 0.0009 0.0004
Vacuole 0.017 0.0025 0.0027 0.0016 0.005 0.003 0.0057 0.0006 0.002 0.0068 0.0029 0.0004 0.0018 0.0102 0.0015 0.0023 0.0028 0.0007
Vacuole Periphery 0.0012 0.0001 0.0002 0.0004 0.0008 0.0002 0.0012 0.0001 0.0008 0.0022 0.0005 0.0002 0.0009 0.0005 0.0003 0.0001 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.8701 27.1139 27.5322 32.2406 23.3882 17.5502 26.6213 30.2868 34.4656 30.8759
Translational Efficiency 0.5967 0.5221 0.6157 0.5552 0.4967 0.7618 0.5833 0.5547 0.4738 0.4797

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
Localization
Cell Percentages cytoplasm (57%), bud neck (11%), mixed (22%)
Cell Cycle Regulation Yes
bud neck - S/G2 (0.00066)
Subcompartmental Group N/A

Pkc1

Pkc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pkc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available