Standard name
Human Ortholog
Description Hexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0.19 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0.07 0.05 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.28 0.4 0.48 0.1 0.17 0.14 0 0.06 0.05 0 0.05 0.07
Mitochondria 0.89 0.93 0.89 0.96 0.85 0.92 0.91 0.89 0.93 0.94 0.95 0.8 0.66 0.53 0.1 0.06 0.05 0.78 0.73 0.76 0.71 0.71 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.08 0 0.06 0.09 0.07 0.12 0.12 0.1 0.14 0.07 0 0 0.07 0.09 0.11 0 0 0 0 0 0
Nucleolus 0.21 0 0.05 0 0 0.1 0.11 0.12 0.13 0.1 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.11 0.08 0 0 0.09 0.14 0.12 0.1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0.06 0 0.07 0 0.06 0 0 0 0 0.15 0.22 0.26 0.16 0.21 0.21 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 0 1 0 0 1 0 0 0 0 1 3 5 2 7 3 0 5 1 3 14 13
Bud 2 5 3 3 1 5 8 9 4 8 1 0 2 3 5 9 8 1 1 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 2 2 5 2 3 0 0 0 1 5 1
Cell Periphery 0 7 6 2 2 1 3 3 0 4 1 8 9 13 11 19 21 0 0 0 0 0 1
Cytoplasm 0 4 6 3 6 4 5 4 0 1 2 0 3 7 31 42 29 0 1 0 0 0 0
Endoplasmic Reticulum 0 5 2 0 0 1 0 0 0 0 0 2 2 4 9 9 8 0 0 0 0 2 1
Endosome 5 0 3 3 8 2 3 9 3 0 0 9 10 9 4 16 10 9 22 9 8 20 20
Golgi 0 2 4 1 5 0 1 0 0 0 0 59 94 136 12 39 24 4 17 11 11 22 34
Mitochondria 112 260 190 134 133 196 242 355 321 234 262 172 156 152 12 13 9 127 219 164 194 303 329
Nucleus 3 6 0 1 0 1 1 4 2 0 1 0 4 0 3 8 2 0 0 0 0 0 0
Nuclear Periphery 2 7 18 3 10 19 18 46 40 26 39 15 5 9 9 19 20 4 3 1 4 2 5
Nucleolus 26 7 10 1 7 22 29 49 45 25 26 7 4 4 1 8 8 2 0 0 2 1 1
Peroxisomes 1 8 1 4 9 1 7 8 5 5 4 9 7 7 7 25 14 6 12 18 38 50 47
SpindlePole 2 3 1 2 2 3 4 7 3 7 3 5 3 7 6 7 9 1 2 0 2 1 1
Vac/Vac Membrane 8 20 12 6 11 8 16 12 6 8 5 33 51 73 20 46 37 2 11 2 1 6 8
Unique Cell Count 126 279 213 140 156 213 266 399 345 248 275 214 237 285 122 223 174 164 302 215 272 429 471
Labelled Cell Count 162 337 256 164 194 263 340 508 434 320 347 320 353 429 133 272 203 164 302 215 272 429 471


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.1 7.3 7.3 8.6 7.5 7.3 7.2 6.3 6.3 6.3 10.0 9.9 9.8 6.5 7.7 8.7 9.4 9.5 10.3
Std Deviation (1e-4) 1.2 1.7 1.4 1.4 3.3 1.8 1.9 1.6 1.7 1.7 1.8 1.6 2.1 1.5 1.3 1.7 2.0 1.8 1.8 2.3
Intensity Change (Log2) -0.01 0.23 0.04 0.0 -0.03 -0.21 -0.21 -0.22 0.45 0.44 0.43 -0.17 0.08 0.24 0.36 0.38 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1.3 0 0 0 0 0 1.7 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.5 0.6 1.0 2.5 2.6 3.6
Cytoplasm 0 0.6 -0.6 0 0 0 0 0 0 0 -0.2 6.3 5.3 4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.1 0
Endosome 0 0 0 0 0 0 0 0 1.7 1.8 1.3 0 2.9 2.4
Golgi 0 0 0 0 0 0 0 0 7.5 9.7 11.3 3.3 5.5 4.5
Mitochondria 2.2 -1.1 1.0 0.6 -0.1 1.6 2.0 2.5 -2.5 -5.9 -8.5 -14.3 -17.4 -16.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.5 -0.7 0.2 -0.7 1.2 1.2 0.7 2.0 -0.6 -3.0 -2.6 -0.3 0 1.0
Nucleolus 0 -0.1 2.2 2.5 3.0 3.2 2.2 2.0 -0.8 -1.8 -2.2 0 -0.6 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 4.7 3.8
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 0.6 -0.9 0.2 -1.6 -2.5 -1.3 -2.3 3.3 4.8 5.9 3.2 4.6 4.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.0368 7.2155 7.6928 6.782 6.6648 7.2032 8.0915 7.7032 8.0219 7.8046 8.1927 8.6719 7.3819 8.7811 8.4833 7.9883 8.6726 8.2322
Actin 0.0281 0.0056 0.0165 0.0129 0.0035 0.0007 0.0212 0.0019 0.0127 0.0013 0.049 0.0012 0.0127 0.0097 0.0088 0.0051 0.003 0.021
Bud 0.0018 0.0006 0.0002 0.0007 0.0002 0 0.0004 0.0003 0.0005 0.0002 0.0004 0 0.0016 0.0027 0.0008 0.0061 0.0011 0.0003
Bud Neck 0.0009 0.0006 0.0006 0.0001 0.0001 0 0.0006 0.0002 0.0003 0.0001 0.0007 0 0.0006 0.0004 0.0002 0.0002 0.0001 0.0003
Bud Periphery 0.0036 0.0015 0.0004 0.0024 0.0003 0.0001 0.0006 0.0003 0.0008 0.0003 0.0005 0.0001 0.0034 0.0049 0.0015 0.0084 0.0047 0.0006
Bud Site 0.0023 0.008 0.0012 0.0015 0.0006 0 0.0027 0.0017 0.0026 0.0001 0.003 0 0.0028 0.0229 0.0024 0.0033 0.0004 0.0006
Cell Periphery 0.0015 0.0014 0.0003 0.0018 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001 0.0003 0.0001 0.0003 0.0009 0.001 0.0004 0.0006 0.0002
Cytoplasm 0.002 0.0002 0.0003 0.0055 0.0053 0 0.0026 0.0006 0.0009 0.0001 0.0012 0 0.0026 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0201 0.003 0.0024 0.0039 0.0148 0.0003 0.0209 0.0106 0.008 0.0177 0.0248 0.005 0.0269 0.0083 0.003 0.009 0.002 0.0056
Eisosomes 0.0014 0.0004 0.0002 0.0012 0.0002 0.0001 0.0006 0.0001 0.0002 0.0001 0.0005 0.0001 0.0004 0.0005 0.0004 0.0002 0.0003 0.0003
Endoplasmic Reticulum 0.0021 0.0002 0.0003 0.0043 0.0016 0 0.0013 0.0002 0.001 0 0.0005 0.0001 0.0048 0.0002 0.0001 0 0.0052 0.0002
Endosome 0.034 0.0181 0.0351 0.009 0.0832 0.0013 0.0202 0.026 0.0109 0.0066 0.0418 0.0043 0.1096 0.01 0.005 0.0107 0.0148 0.0095
Golgi 0.0426 0.0284 0.0341 0.0322 0.0243 0.0039 0.0195 0.039 0.0189 0.0131 0.0396 0.0043 0.0325 0.0131 0.0051 0.0358 0.0244 0.0207
Lipid Particles 0.0381 0.0104 0.0073 0.0057 0.0432 0.0031 0.026 0.0201 0.0069 0.0259 0.0111 0.0065 0.0614 0.022 0.0091 0.0195 0.0268 0.0438
Mitochondria 0.7748 0.8978 0.8616 0.909 0.7807 0.9864 0.7494 0.8474 0.8987 0.9181 0.8033 0.9558 0.6665 0.8075 0.9083 0.8633 0.886 0.8617
None 0.0015 0.0001 0.0001 0.0016 0.0005 0 0.0279 0.0001 0.0036 0.0001 0.0003 0.0001 0.0017 0.0002 0.0001 0.0001 0.0002 0.0001
Nuclear Periphery 0.0033 0.0001 0.0002 0.0004 0.0005 0.0001 0.0075 0.0002 0.0038 0 0.0008 0.0002 0.0242 0.0017 0.0001 0 0.0019 0.0006
Nucleolus 0.0005 0.0094 0.0057 0.0001 0.0002 0.0001 0.0096 0.0003 0.0074 0.0001 0.0023 0.0001 0.0012 0.0009 0.0026 0.0008 0.0005 0.0003
Nucleus 0.0006 0.0007 0.0006 0.0002 0.0002 0.0001 0.0268 0.0002 0.0008 0 0.0018 0 0.0025 0.0007 0.0003 0.0002 0.0002 0.0001
Peroxisomes 0.015 0.0036 0.0128 0.0009 0.0325 0.0017 0.0205 0.0292 0.0159 0.0143 0.0086 0.0152 0.0183 0.0246 0.0279 0.0261 0.0048 0.0245
Punctate Nuclear 0.0087 0 0.0001 0.0002 0.0004 0 0.0362 0.0001 0.0009 0.0002 0.0036 0.0012 0.0046 0.0001 0 0.0002 0 0.0002
Vacuole 0.0095 0.0038 0.011 0.0026 0.0028 0.0003 0.0029 0.0101 0.002 0.0004 0.0041 0.0006 0.0108 0.026 0.0163 0.004 0.0078 0.0014
Vacuole Periphery 0.0077 0.0062 0.0091 0.004 0.0048 0.0018 0.0024 0.0112 0.0029 0.001 0.0016 0.005 0.0107 0.0428 0.0071 0.0063 0.0151 0.0079

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.1146 17.3414 15.4416 24.3994 19.1151 23.2657 24.0213 24.1417 29.8983 25.6597
Translational Efficiency 0.7773 0.8742 0.9241 0.7357 0.8538 0.6476 0.6741 0.7752 0.6691 0.699

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
132 1307 2493 1219 1711 834 2422 1628 1843 2141 4915 2847

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1186.74 948.37 1247.07 1149.46 865.99 929.11 1108.84 1101.95 888.96 940.87 1178.95 1122.29
Standard Deviation 177.11 166.74 201.25 176.69 135.53 140.81 176.15 165.92 161.68 157.43 201.52 172.23
Intensity Change Log 2 -0.323482 0.071539 -0.046048 0.101499 0.356629 0.347636 -0.128746 0.198741 0.133285

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.010123 0.005317 0.001112 0.005473 0.001067 0.002225 0.003597 0.007085 0.001716 0.004112 0.002337 0.006395
Bud Neck 0.005947 0.001568 0.000516 0.001560 0.000796 0.001033 0.000767 0.001085 0.001165 0.001359 0.000639 0.001288
Bud Site 0.020972 0.002766 0.000668 0.008726 0.001989 0.002142 0.002676 0.004796 0.003349 0.002523 0.001657 0.006479
Cell Periphery 0.003310 0.000199 0.000071 0.000431 0.000303 0.000149 0.000153 0.000450 0.000518 0.000179 0.000112 0.000442
Cytoplasm 0.001713 0.001134 0.000279 0.001033 0.000461 0.000283 0.000759 0.000696 0.000551 0.000802 0.000516 0.000840
Cytoplasmic Foci 0.049702 0.020506 0.017869 0.015881 0.009427 0.013106 0.010794 0.012696 0.012312 0.017623 0.014382 0.014059
Eisosomes 0.000205 0.000119 0.000045 0.000148 0.000038 0.000062 0.000075 0.000110 0.000050 0.000097 0.000060 0.000126
Endoplasmic Reticulum 0.005408 0.000162 0.000156 0.000483 0.000185 0.000110 0.000109 0.000395 0.000559 0.000142 0.000133 0.000433
Endosome 0.097473 0.024160 0.008414 0.019265 0.016802 0.017274 0.009015 0.019112 0.022580 0.021477 0.008710 0.019178
Golgi 0.186826* 0.033681 0.041358 0.055717 0.011719 0.021806 0.021547 0.050077 0.024260 0.029055 0.031595 0.052492
Lipid Particles 0.000958 0.007745 0.003616 0.001137 0.004515 0.003005 0.001615 0.001364 0.004260 0.005898 0.002630 0.001267
Mitochondria 0.423629* 0.858726* 0.885610* 0.852144* 0.928539* 0.908737* 0.926295* 0.871116* 0.892376* 0.878207* 0.905659* 0.862993*
Mitotic Spindle 0.009616 0.002004 0.004297 0.006551 0.001073 0.001188 0.004013 0.003423 0.001685 0.001686 0.004157 0.004763
None 0.000346 0.000345 0.000783 0.000425 0.000196 0.000183 0.000262 0.000328 0.000207 0.000282 0.000526 0.000370
Nuclear Periphery 0.000916 0.000207 0.001089 0.000331 0.000109 0.000051 0.000120 0.000310 0.000167 0.000146 0.000611 0.000319
Nuclear Periphery Foci 0.002687 0.000496 0.000577 0.000188 0.000106 0.000121 0.000116 0.000254 0.000290 0.000350 0.000350 0.000226
Nucleolus 0.000215 0.000361 0.000118 0.000092 0.000036 0.000236 0.000060 0.000062 0.000049 0.000312 0.000089 0.000075
Nucleus 0.000244 0.000123 0.000040 0.000039 0.000026 0.000375 0.000036 0.000071 0.000041 0.000221 0.000038 0.000058
Peroxisomes 0.010866 0.035252 0.004197 0.010589 0.017505 0.022479 0.012993 0.011442 0.017030 0.030276 0.008532 0.011076
Vacuole 0.100246 0.002370 0.008160 0.009379 0.001912 0.002328 0.002157 0.006006 0.008955 0.002354 0.005202 0.007450
Vacuole Periphery 0.068598 0.002763 0.021027 0.010410 0.003196 0.003110 0.002839 0.009120 0.007880 0.002898 0.012064 0.009672

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.79 3.50 1.74 -0.16 -5.76 -2.11 -4.38 -5.38 -4.11 -2.91 -3.99 -1.51 -6.11 -2.65 -5.46
Bud Neck 2.34 2.95 2.35 -0.01 -3.52 -0.60 0.12 -0.82 -0.12 -1.01 -0.58 2.24 -0.42 0.20 -2.97
Bud Site 3.73 4.19 2.41 -3.71 -5.47 -0.14 -0.91 -2.80 -2.08 -2.01 1.04 2.60 -3.17 -4.08 -5.62
Cell Periphery 3.19 3.33 2.95 -3.42 -5.99 1.73 1.66 -1.03 -2.62 -2.56 3.08 3.74 0.59 -3.60 -4.66
Cytoplasm 0.85 4.83 2.08 0.17 -5.50 1.30 -1.21 -1.41 -3.81 0.28 -0.65 0.19 -1.94 -0.10 -2.40
Cytoplasmic Foci 4.13 4.55 4.81 2.22 1.10 -1.90 -1.02 -2.30 0.21 -1.39 -3.40 -1.60 -1.29 2.52 0.30
Eisosomes 2.49 4.85 0.95 -0.55 -2.03 -3.34 -6.38 -8.32 -4.66 -3.59 -6.05 -2.13 -3.37 -1.26 -2.95
Endoplasmic Reticulum 2.71 2.71 2.54 -4.08 -4.15 0.95 0.98 -2.39 -5.99 -6.12 2.63 2.68 0.77 -6.73 -6.98
Endosome 6.61 8.15 7.09 1.90 -6.27 -0.20 4.49 -1.06 -0.78 -6.05 0.49 7.70 1.63 1.27 -8.69
Golgi 7.41 7.05 6.27 -4.71 -3.16 -3.49 -5.27 -10.99 -6.61 -7.78 -1.75 -3.00 -8.60 -7.46 -7.23
Lipid Particles -5.19 -4.83 -0.43 5.16 5.13 1.39 3.56 3.77 2.03 0.68 -1.49 2.12 3.98 5.47 4.39
Mitochondria -13.07 -14.09 -12.84 0.65 3.84 2.28 0.39 7.48 3.92 7.61 1.82 -2.05 3.96 2.15 7.61
Mitotic Spindle 2.43 1.68 0.95 -4.76 -2.11 -0.24 -4.48 -3.54 -2.92 0.67 -0.00 -4.79 -5.10 -5.21 -0.88
None 0.00 -8.14 -1.67 -1.06 9.27 0.53 -4.09 -6.16 -5.35 -3.38 -1.61 -14.81 -8.92 -1.87 7.15
Nuclear Periphery 1.27 -0.29 1.05 -0.88 3.12 2.35 -0.30 -2.91 -3.90 -2.63 0.27 -3.62 -2.15 -2.06 2.29
Nuclear Periphery Foci 1.08 1.05 1.25 1.04 3.50 -0.32 -0.31 -2.49 -1.87 -2.18 -0.26 -0.39 0.43 0.67 1.88
Nucleolus -0.74 1.40 1.83 1.43 0.81 -1.65 -1.64 -1.03 1.41 -0.09 -2.13 -2.42 -1.44 1.91 0.66
Nucleus 1.76 3.51 3.54 2.19 0.07 -1.19 -1.08 -1.31 1.03 -0.98 -1.54 0.39 -0.79 1.38 -0.94
Peroxisomes -6.08 2.60 0.09 7.17 -4.22 -1.32 1.96 2.45 3.02 0.74 -4.58 4.49 2.88 7.50 -1.92
Vacuole 5.74 5.39 5.31 -5.50 -0.95 -0.67 -0.70 -5.22 -3.94 -4.93 4.68 2.63 0.93 -6.85 -3.00
Vacuole Periphery 6.58 4.72 5.78 -6.56 6.26 0.07 0.63 -6.60 -4.70 -7.79 4.81 -3.65 -1.60 -8.61 2.55
Standard name
Human Ortholog
Description Hexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Coq1

Coq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available