Standard name
Human Ortholog
Description Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0
Bud 0.06 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0.09 0.12 0.15 0 0 0 0 0 0
Cytoplasm 0.9 0.92 0.97 0.97 0.96 0.94 0.91 – 0.82 0.82 0.8 0.85 0.96 0.96 0.96 0.96 0.95 0.94 0.88 0.83 0.85 0.62 0.65 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.09 – 0.25 0.23 0.23 0.17 0 0 0 0 0 0 0 0 0 0.08 0.06 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 4 8 6 11 3 7 – 3 5 4 2 7 10 4 1 2 2 2 5 7 16 31 21
Bud 7 8 5 6 10 6 15 – 14 26 17 16 12 9 16 5 2 4 2 10 8 11 18 20
Bud Neck 0 1 7 2 3 6 3 – 4 0 3 1 0 0 1 0 0 0 3 5 7 23 22 18
Bud Site 1 1 1 0 0 0 1 – 3 0 4 1 0 2 1 0 0 0 – – – – – –
Cell Periphery 1 1 13 9 17 16 9 – 12 15 13 16 17 6 19 28 25 34 0 0 3 4 11 10
Cytoplasm 108 197 354 324 472 455 615 – 424 549 284 505 343 492 640 296 198 207 108 196 316 187 313 465
Endoplasmic Reticulum 1 5 0 0 1 2 5 – 1 3 4 3 1 2 2 15 4 13 0 1 3 4 14 8
Endosome 0 0 0 1 0 0 1 – 0 3 0 0 2 0 1 0 0 0 0 1 2 3 3 4
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0
Mitochondria 4 7 1 1 3 33 60 – 127 156 82 100 4 2 7 3 3 1 0 7 11 22 30 18
Nucleus 0 0 1 3 2 1 2 – 4 4 3 4 0 0 1 3 4 0 0 0 1 0 0 0
Nuclear Periphery 0 1 0 1 0 0 0 – 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 – 1 1 0 0 0 4 3 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 1 4 1 4 6 – 1 6 1 7 1 5 1 0 1 1 0 0 1 0 1 5
Vac/Vac Membrane 0 0 3 0 2 2 4 – 3 9 1 5 1 0 1 3 2 3 0 2 5 21 25 25
Unique Cell Count 120 213 365 334 492 486 679 514 667 354 594 358 510 665 308 209 221 123 237 373 300 481 610
Labelled Cell Count 128 227 394 357 522 528 728 597 779 416 661 388 533 697 354 242 266 123 237 373 300 481 610


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.0 5.9 5.5 6.2 4.9 4.9 – 4.2 4.3 4.2 4.7 5.2 5.0 5.2 7.5 7.8 7.7 4.8 4.7 5.4
Std Deviation (1e-4) 0.6 0.7 1.1 1.6 1.5 1.0 0.8 – 0.6 0.8 0.8 0.8 0.8 0.7 1.0 1.0 1.0 1.1 1.1 0.9 1.2
Intensity Change (Log2) – – – -0.09 0.08 -0.25 -0.27 – -0.48 -0.46 -0.47 -0.33 -0.18 -0.23 -0.17 0.36 0.4 0.39 -0.29 -0.32 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.4 0 0 -1.5 – 0 0 0 0 -0.2 -0.2 0 0 0 0
Bud 0.5 0.7 0 0.9 – 0 0 0 0 1.8 0.5 1.1 0 0 0
Bud Neck 0 0 -0.8 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -0.1 -0.2 -2.4 – 0 0 0 0 0.8 -2.4 -0.6 3.0 3.9 5.1
Cytoplasm 0 -0.8 -2.2 -3.8 – -6.6 -6.8 -7.1 -5.9 -0.8 -0.4 -0.6 -0.6 -1.4 -1.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 4.3 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.8 5.6 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4691 0.6975 0.5535 0.731 0.1471 0.4358 -0.1114 -0.0812 0.3884 0.6774 0.2542 0.3555 0.2097 0.3632 0.397 0.5981 0.7109 0.3316
Actin 0.1129 0.0017 0.0074 0.0206 0.017 0.0046 0.0098 0.0019 0.0166 0.0018 0.0029 0.0204 0.0135 0.0078 0.0453 0.0231 0.009 0.0048
Bud 0.0079 0.0096 0.0127 0.0021 0.0006 0.003 0.0012 0.0055 0.0039 0.0046 0.0019 0.001 0.0118 0.0021 0.0553 0.0003 0.0019 0.0001
Bud Neck 0.0026 0.0009 0.0319 0.0006 0.0005 0.0303 0.0014 0.0015 0.0022 0.0009 0.0007 0.0464 0.0009 0.0012 0.0042 0.0008 0.0246 0.0308
Bud Periphery 0.0183 0.0148 0.0219 0.0084 0.0007 0.0047 0.0019 0.0095 0.0048 0.0079 0.0027 0.0014 0.0124 0.0019 0.0606 0.0002 0.0031 0.0001
Bud Site 0.0154 0.0219 0.028 0.0013 0.0012 0.0014 0.0097 0.0294 0.0128 0.002 0.0019 0.0009 0.0203 0.0331 0.03 0.0007 0.0121 0.0001
Cell Periphery 0.0022 0.0011 0.0011 0.0004 0.0002 0.0006 0.0006 0.001 0.0005 0.0004 0.0001 0.0003 0.0005 0.001 0.001 0.0001 0.0008 0.0002
Cytoplasm 0.2705 0.4012 0.3838 0.5402 0.4893 0.461 0.59 0.4977 0.649 0.6209 0.6165 0.7128 0.5049 0.5869 0.532 0.6523 0.6595 0.7805
Cytoplasmic Foci 0.0253 0.0184 0.0147 0.0173 0.0177 0.017 0.048 0.0307 0.0236 0.0307 0.0372 0.0144 0.0179 0.0147 0.0175 0.032 0.0225 0.0053
Eisosomes 0.0007 0.0002 0.0007 0.0006 0.0001 0.0001 0.0004 0.0003 0.0002 0.0001 0 0.0001 0.0004 0.0005 0.0005 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0058 0.002 0.0076 0.005 0.0025 0.0033 0.0034 0.0013 0.0026 0.0026 0.0018 0.0023 0.0144 0.0034 0.0045 0.0023 0.0028 0.0045
Endosome 0.0403 0.0053 0.0074 0.0128 0.0106 0.0112 0.0213 0.0024 0.0249 0.0264 0.0909 0.0094 0.0373 0.0045 0.0189 0.0439 0.0546 0.0044
Golgi 0.0147 0.0006 0.0011 0.0027 0.0027 0.005 0.007 0.001 0.0071 0.0114 0.0265 0.0029 0.0043 0.0016 0.005 0.0132 0.0168 0.0005
Lipid Particles 0.0115 0.001 0.004 0.0036 0.002 0.0019 0.0131 0.001 0.0014 0.0089 0.0012 0.0007 0.0085 0.001 0.0037 0.0206 0.021 0.0005
Mitochondria 0.0052 0.0006 0.0035 0.0033 0.0012 0.0026 0.0139 0.0008 0.0074 0.0186 0.0129 0.0123 0.016 0.001 0.0127 0.0025 0.0096 0.0005
None 0.4301 0.5131 0.4487 0.3561 0.4266 0.44 0.235 0.4073 0.2329 0.2445 0.1878 0.1604 0.2557 0.3323 0.1902 0.1781 0.1071 0.1598
Nuclear Periphery 0.0038 0.0006 0.0046 0.0058 0.0049 0.0021 0.0026 0.0004 0.0007 0.0011 0.0009 0.006 0.0371 0.0007 0.0012 0.0023 0.005 0.0035
Nucleolus 0.0004 0.0007 0.002 0.0007 0.0002 0.0003 0.0009 0.0004 0.0003 0.0003 0.0001 0.0001 0.0023 0.0003 0.0003 0.0004 0.0022 0.0002
Nucleus 0.0017 0.0012 0.0056 0.0022 0.0016 0.0022 0.0034 0.0012 0.0012 0.002 0.0013 0.0018 0.0185 0.0014 0.0012 0.001 0.0046 0.0018
Peroxisomes 0.0116 0.0005 0.0033 0.0082 0.0032 0.0007 0.0249 0.0032 0.0024 0.005 0.0021 0.0037 0.0075 0.0015 0.0042 0.0075 0.0121 0.0001
Punctate Nuclear 0.0096 0.0013 0.0023 0.0058 0.015 0.0044 0.0057 0.0018 0.0008 0.0011 0.0005 0.0007 0.0049 0.0009 0.0012 0.0069 0.0014 0.0009
Vacuole 0.0083 0.0029 0.0062 0.0019 0.002 0.003 0.0045 0.0016 0.0042 0.0069 0.0075 0.0015 0.0075 0.002 0.0087 0.0078 0.0231 0.0009
Vacuole Periphery 0.0011 0.0003 0.0013 0.0005 0.0004 0.0007 0.0012 0.0001 0.0008 0.0019 0.0026 0.0007 0.0034 0.0002 0.0019 0.0035 0.0058 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9697 5.3535 6.6208 8.6084 13.9114 14.4645 9.6981 8.9811 9.0583 15.6539
Translational Efficiency 0.8096 1.8738 0.7804 0.7266 0.781 0.8974 0.9876 0.8753 0.8303 0.8526

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1029 1465 2003 1115 1017 1154 2659 631 2046 2619 4662 1746

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 562.34 672.52 743.61 875.60 555.16 692.43 843.68 627.09 558.77 681.29 800.69 785.79
Standard Deviation 93.51 124.21 175.17 104.19 92.54 139.53 93.24 219.30 93.10 131.56 143.51 196.38
Intensity Change Log 2 — 0.258135 0.403104 0.638829 — 0.318765 0.603792 0.175769 — 0.288573 0.506291 0.427273

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000217 0.000505 0.000514 0.002134 0.000339 0.000802 0.000802 0.000337 0.000278 0.000636 0.000678 0.001484
Bud Neck 0.033913 0.020994 0.016738 0.008882 0.051300 0.040808 0.017579 0.041409 0.042556 0.029724 0.017218 0.020638
Bud Site 0.027146 0.030618 0.044322 0.069110 0.027372 0.041674 0.056559 0.040313 0.027259 0.035490 0.051301 0.058703
Cell Periphery 0.000537 0.000195 0.000170 0.000270 0.000361 0.000362 0.000116 0.004352 0.000450 0.000269 0.000139 0.001745
Cytoplasm 0.521066* 0.579460* 0.696469* 0.559286* 0.478958* 0.520915* 0.693592* 0.598840* 0.500136* 0.553664* 0.694828* 0.573581*
Cytoplasmic Foci 0.179040 0.156979 0.032009 0.033524 0.226015* 0.171652 0.024213 0.069065 0.202390* 0.163445 0.027562 0.046368
Eisosomes 0.000656 0.000172 0.000043 0.000089 0.000621 0.000355 0.000045 0.004520 0.000639 0.000252 0.000044 0.001691
Endoplasmic Reticulum 0.002209 0.000888 0.000771 0.001982 0.001171 0.000566 0.000426 0.000454 0.001693 0.000746 0.000575 0.001430
Endosome 0.016532 0.016822 0.002655 0.003197 0.027497 0.025020 0.001607 0.005088 0.021982 0.020434 0.002057 0.003881
Golgi 0.003645 0.002253 0.000342 0.001079 0.004945 0.004737 0.000163 0.003679 0.004291 0.003347 0.000240 0.002019
Lipid Particles 0.011280 0.005090 0.001142 0.001048 0.013259 0.008299 0.000411 0.004880 0.012263 0.006504 0.000725 0.002433
Mitochondria 0.002594 0.002033 0.000830 0.004063 0.004272 0.004853 0.000484 0.000489 0.003428 0.003275 0.000633 0.002771
Mitotic Spindle 0.000288 0.000580 0.006340 0.013313 0.000862 0.004276 0.009668 0.001202 0.000573 0.002208 0.008238 0.008936
None 0.019201 0.007043 0.010767 0.004407 0.013594 0.017789 0.001314 0.056428 0.016414 0.011778 0.005376 0.023207
Nuclear Periphery 0.000840 0.000458 0.000380 0.000483 0.000332 0.000242 0.000200 0.000194 0.000587 0.000363 0.000277 0.000379
Nuclear Periphery Foci 0.001238 0.000699 0.001029 0.001728 0.000518 0.000397 0.000379 0.003695 0.000880 0.000566 0.000658 0.002439
Nucleolus 0.001794 0.001645 0.000863 0.000126 0.001572 0.001912 0.000111 0.001224 0.001684 0.001763 0.000434 0.000523
Nucleus 0.090947 0.070105 0.115474 0.088455 0.058390 0.058724 0.070772 0.063987 0.074764 0.065090 0.089978 0.079612
Peroxisomes 0.003078 0.004426 0.001418 0.001410 0.005236 0.009840 0.001526 0.003331 0.004151 0.006811 0.001480 0.002104
Vacuole 0.082599 0.097512 0.066875 0.203261* 0.081972 0.085373 0.119872 0.096259 0.082288 0.092163 0.097102 0.164591
Vacuole Periphery 0.001179 0.001522 0.000849 0.002154 0.001414 0.001404 0.000160 0.000252 0.001296 0.001470 0.000456 0.001467

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.38 -5.83 -5.47 -3.76 -3.66 -2.93 -4.45 0.48 3.06 4.42 -3.67 -7.00 -5.14 -2.34 -2.34
Bud Neck 3.81 6.21 9.09 8.16 4.96 2.13 8.77 3.67 2.11 -4.77 4.33 10.74 9.44 7.02 -0.77
Bud Site -0.77 -1.99 -1.85 -1.33 -0.03 -2.96 -3.28 -0.94 1.76 1.77 -2.59 -3.82 -2.10 0.27 1.26
Cell Periphery 5.66 7.28 6.17 -0.17 -2.21 0.05 10.59 -5.32 -5.30 -5.84 4.29 11.21 -4.18 -5.12 -5.95
Cytoplasm -5.37 -12.29 4.93 10.22 16.98 -3.54 -17.07 -8.39 -5.37 3.90 -6.65 -20.79 -1.33 4.54 16.39
Cytoplasmic Foci 3.45 28.50 29.14 28.92 1.55 6.94 33.76 23.60 17.54 -8.50 7.69 44.02 38.40 33.68 -6.52
Eisosomes 5.81 8.43 7.90 1.99 -8.63 3.29 7.88 -4.05 -4.49 -4.97 6.60 11.54 -2.45 -4.12 -5.01
Endoplasmic Reticulum 3.60 3.02 0.58 -5.07 -4.82 1.91 2.14 2.59 1.53 2.23 3.87 3.90 1.14 -4.69 -5.57
Endosome -0.12 14.79 15.26 15.07 2.02 1.01 15.48 13.61 10.67 -6.69 1.19 20.73 19.63 17.27 -3.80
Golgi 3.22 8.60 7.80 7.33 -0.82 0.27 9.98 1.46 1.24 -1.70 2.12 13.20 4.90 3.29 -1.87
Lipid Particles 5.67 10.79 10.63 6.41 -1.55 2.73 9.44 6.74 3.51 -5.97 5.57 13.97 12.00 6.48 -6.93
Mitochondria 1.08 3.77 1.26 0.68 -0.87 -0.44 4.60 4.62 4.43 0.36 0.25 5.95 3.40 3.19 -0.98
Mitotic Spindle -1.72 -6.65 -4.64 -4.44 -0.27 -2.15 -5.81 -1.12 1.62 5.47 -2.26 -8.39 -4.54 -2.62 2.33
None 4.07 3.88 5.81 2.76 4.11 -0.89 5.12 -4.23 -3.41 -6.61 1.98 6.56 0.01 -1.72 -5.26
Nuclear Periphery 0.80 0.53 0.18 -2.65 -2.81 1.47 -0.39 0.85 -0.88 1.97 0.93 0.56 0.00 -3.26 -3.54
Nuclear Periphery Foci 3.72 0.81 -3.06 -8.13 -4.52 1.01 1.20 -3.16 -3.34 -3.35 3.36 1.90 -4.55 -5.90 -5.37
Nucleolus 0.53 5.00 9.99 5.97 3.05 -0.61 4.91 2.08 2.09 -5.57 -0.25 7.31 7.06 5.23 -0.84
Nucleus 4.05 -9.01 -3.79 -8.24 5.28 -0.04 -7.06 -1.55 -1.59 4.45 2.90 -10.39 -5.34 -8.35 3.96
Peroxisomes -2.50 5.19 7.16 8.10 2.41 -3.48 7.88 1.83 3.92 -0.97 -4.01 9.44 4.75 6.94 -0.29
Vacuole -3.71 -5.85 -27.40 -25.44 -23.85 -1.13 -17.33 -7.74 -7.11 2.86 -3.68 -17.33 -26.67 -24.79 -16.72
Vacuole Periphery -2.16 1.56 1.59 2.63 -0.27 0.10 13.17 11.50 6.62 -1.34 -1.32 5.74 4.09 4.57 -0.87
Standard name
Human Ortholog
Description Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dsf2

Dsf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dsf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available