Standard name
Human Ortholog
Description Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.37 0.54 0.65 0.64 0.71 0.79 – 0.82 0.86 0.85 0.84 0.79 0.9 0.97 0.21 0.13 0.19 0 0 0 0.08 0.06 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.1 0 0 0 0.08 0 – 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.11 0.08 0.06 0.05 0 0.1 0 – 0 0 0 0 0 0 0 0.42 0.39 0.35 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.67 0.58 0.59 0.6 0.32 0.4 – 0.28 0.22 0.21 0.22 0.3 0.12 0.05 0.49 0.6 0.61 0.89 0.87 0.89 0.85 0.82 0.7
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud 0 1 0 0 1 4 0 – 5 2 6 3 0 0 0 0 0 0 3 7 1 1 2 7
Bud Neck 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 67 237 119 140 150 179 321 – 380 446 410 406 130 244 280 58 39 44 5 24 9 20 18 24
Endoplasmic Reticulum 3 2 2 1 0 0 0 – 1 0 0 1 1 1 0 15 18 11 0 2 1 2 2 4
Endosome 19 11 9 3 7 0 5 – 2 4 2 5 3 6 2 5 14 5 16 20 3 4 10 13
Golgi 4 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 2 0 3 4 1 0 0 1
Mitochondria 41 61 2 3 0 19 5 – 14 18 18 40 1 0 0 10 6 10 5 9 3 2 10 10
Nucleus 42 49 14 11 9 24 10 – 10 9 4 3 0 0 0 117 115 82 2 7 0 1 0 5
Nuclear Periphery 1 3 0 1 0 1 0 – 2 0 1 2 0 1 0 0 0 1 1 1 0 1 1 5
Nucleolus 0 0 0 0 0 0 0 – 1 1 0 1 0 0 0 0 1 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 – 0 0 1 1 0 1 0 0 1 1 3 3 1 0 2 4
Vac/Vac Membrane 285 430 128 126 142 80 161 – 130 115 103 105 50 33 15 137 180 141 344 598 221 209 242 205
Unique Cell Count 366 639 220 214 235 252 404 466 517 483 485 165 272 288 281 298 231 389 685 250 247 295 293
Labelled Cell Count 462 795 274 286 310 307 503 545 595 546 569 185 286 297 342 376 297 389 685 250 247 295 293


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.7 5.8 5.8 7.4 4.7 5.7 – 4.9 5.0 4.9 4.8 5.3 5.2 5.4 7.5 8.2 7.0 4.7 5.0 5.6
Std Deviation (1e-4) 1.1 1.0 1.6 1.3 6.4 1.7 1.7 – 1.4 1.4 1.2 1.0 1.5 2.0 1.9 2.1 2.3 1.9 1.3 1.7 2.4
Intensity Change (Log2) – – – 0.0 0.35 -0.3 -0.03 – -0.23 -0.21 -0.23 -0.25 -0.14 -0.15 -0.11 0.39 0.51 0.27 -0.31 -0.22 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -2.1 0.5 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2182 1.269 0.9773 0.5163 0.5387 1.1376 1.7956 3.6024 2.7615 2.3774 2.4512 2.7989 3.6608 5.5461 4.7124 5.0208 4.8643 5.2253
Actin 0.0064 0 0.02 0 0.0001 0.0014 0.0069 0 0 0 0 0.0015 0 0 0.0001 0 0 0
Bud 0.0005 0.0004 0.0006 0.0004 0.0004 0.0006 0.0003 0.0004 0.0004 0.0003 0.0004 0.0004 0.0002 0.0003 0.0003 0.0002 0.0002 0.0002
Bud Neck 0.0008 0.001 0.0024 0.0008 0.0007 0.002 0.0009 0.0015 0.0014 0.0005 0.0012 0.0019 0.0005 0.0007 0.0043 0.0006 0.0011 0.0009
Bud Periphery 0.001 0.0008 0.0014 0.0006 0.001 0.0015 0.0009 0.001 0.001 0.0007 0.0012 0.0012 0.0006 0.0009 0.0009 0.0005 0.0009 0.0007
Bud Site 0.0011 0.0015 0.0025 0.004 0.0007 0.0014 0.0006 0.0013 0.0014 0.0005 0.001 0.001 0.0005 0.0006 0.0016 0.0004 0.0004 0.0005
Cell Periphery 0.0011 0.0022 0.0019 0.0012 0.0009 0.0029 0.0013 0.0023 0.0016 0.0007 0.0025 0.0022 0.0018 0.0023 0.0018 0.0016 0.0037 0.0024
Cytoplasm 0.0155 0.0096 0.0106 0.0085 0.0023 0.0044 0.0044 0.0043 0.0056 0.0011 0.0016 0.0033 0.0111 0.0053 0.0036 0.0022 0.001 0.0036
Cytoplasmic Foci 0.0261 0.005 0.0092 0.0111 0.0028 0.002 0.0086 0.0068 0.0065 0.0051 0.0037 0.0068 0.0059 0.0103 0.0065 0.0047 0.0005 0.0034
Eisosomes 0.0001 0 0.0003 0 0 0 0.0002 0 0 0 0 0 0.0001 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0083 0.0027 0.0068 0.0021 0.0038 0.0032 0.0023 0.0032 0.0029 0.0007 0.0014 0.0022 0.0032 0.0022 0.0034 0.0009 0.0016 0.0017
Endosome 0.2472 0.1563 0.2272 0.3802 0.2285 0.1132 0.2416 0.1303 0.2177 0.2109 0.1524 0.1973 0.2312 0.1357 0.2085 0.1173 0.0763 0.1146
Golgi 0.0077 0.0022 0.0188 0.0061 0.0046 0.0028 0.0072 0.0029 0.0064 0.0072 0.0025 0.0045 0.0048 0.0025 0.0075 0.0013 0.0011 0.0022
Lipid Particles 0.0163 0.0036 0.0267 0.0079 0.0136 0.0031 0.0163 0.0048 0.0115 0.0043 0.0072 0.0112 0.0109 0.0221 0.0201 0.0059 0.0018 0.0101
Mitochondria 0.0065 0.0023 0.0267 0.0024 0.004 0.0035 0.018 0.0118 0.0302 0.0069 0.0042 0.0125 0.0052 0.0123 0.0136 0.002 0.0007 0.0176
None 0.035 0.0005 0.0268 0.0006 0.0002 0.001 0.0092 0.0085 0.0014 0.0001 0.0002 0.0006 0.0149 0.0209 0.0016 0.0161 0 0.0024
Nuclear Periphery 0.0172 0.008 0.0354 0.0025 0.0289 0.0178 0.0071 0.0064 0.0091 0.0015 0.0026 0.0062 0.008 0.0035 0.0055 0.0022 0.0029 0.0037
Nucleolus 0.0021 0.0033 0.0071 0.0031 0.0018 0.0037 0.0032 0.0047 0.0036 0.0013 0.0029 0.0036 0.0031 0.0026 0.0028 0.0025 0.0023 0.0038
Nucleus 0.017 0.0085 0.0141 0.0039 0.0031 0.0148 0.0064 0.0042 0.0055 0.0021 0.0037 0.004 0.0052 0.0023 0.002 0.0021 0.0031 0.0031
Peroxisomes 0.0047 0.0002 0.0025 0.0011 0.0005 0.0005 0.0043 0.0008 0.0011 0.0009 0.0005 0.0015 0.0004 0.0009 0.0024 0.0005 0.0002 0.0003
Punctate Nuclear 0.0054 0.0001 0.0025 0.0002 0.0005 0.0044 0.0006 0.0001 0.0009 0 0 0.0005 0.0005 0.0004 0.0002 0.0003 0 0.0001
Vacuole 0.4345 0.6344 0.428 0.4788 0.5957 0.6289 0.4919 0.6301 0.52 0.5952 0.6581 0.559 0.5368 0.6083 0.5533 0.6698 0.7564 0.6289
Vacuole Periphery 0.1454 0.1572 0.1286 0.0843 0.1059 0.1867 0.1677 0.1747 0.1718 0.16 0.1527 0.1784 0.155 0.1658 0.16 0.1691 0.1457 0.1997

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.0442 37.0697 16.7436 28.0224 24.5448 28.8312 34.6293 41.2455 39.3278 36.712
Translational Efficiency 2.1412 1.625 2.3275 1.566 1.6031 2.2085 2.0878 1.2791 1.4894 1.3785

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1975 1006 2194 1524 1444 2430 519 1158 3419 3436 2713 2682

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 741.98 753.63 825.15 788.91 659.02 802.78 875.37 819.33 706.94 788.39 834.76 802.04
Standard Deviation 92.65 99.16 98.46 108.07 85.11 98.57 96.77 115.36 98.47 101.25 100.11 112.29
Intensity Change Log 2 — 0.022476 0.153276 0.088480 — 0.284682 0.409571 0.314122 — 0.151765 0.279519 0.199026

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000024 0.000068 0.000168 0.000293 0.000030 0.000088 0.000217 0.000602 0.000027 0.000082 0.000177 0.000427
Bud Neck 0.015572 0.024650 0.033885 0.029245 0.022489 0.019089 0.028128 0.031482 0.018493 0.020717 0.032784 0.030211
Bud Site 0.006700 0.021784 0.013733 0.032827 0.007344 0.014654 0.019472 0.044163 0.006972 0.016742 0.014831 0.037722
Cell Periphery 0.000347 0.000273 0.000061 0.000077 0.000128 0.000294 0.000108 0.000069 0.000254 0.000288 0.000070 0.000073
Cytoplasm 0.039305 0.122705* 0.353641* 0.153670* 0.164468* 0.047097 0.190900* 0.235775* 0.092167 0.069234 0.322508* 0.189120*
Cytoplasmic Foci 0.018002 0.059074 0.034986 0.060270 0.056154 0.019262 0.064988 0.080133 0.034116 0.030918 0.040725 0.068846
Eisosomes 0.000020 0.000029 0.000029 0.000027 0.000037 0.000023 0.000069 0.000030 0.000028 0.000025 0.000036 0.000028
Endoplasmic Reticulum 0.001676 0.001324 0.003059 0.001336 0.001295 0.002728 0.003466 0.000871 0.001515 0.002317 0.003137 0.001135
Endosome 0.052790 0.091796 0.049304 0.175745 0.069722 0.062667 0.128042 0.143690 0.059941 0.071195 0.064367 0.161905
Golgi 0.000881 0.001636 0.000452 0.003469 0.000832 0.001580 0.000503 0.005804 0.000860 0.001597 0.000462 0.004477
Lipid Particles 0.001270 0.001514 0.003389 0.002815 0.003058 0.001247 0.004625 0.002202 0.002025 0.001325 0.003626 0.002551
Mitochondria 0.002073 0.001723 0.001784 0.002247 0.001437 0.002201 0.000611 0.005528 0.001804 0.002061 0.001559 0.003664
Mitotic Spindle 0.000193 0.000408 0.020462 0.019444 0.001719 0.001499 0.006594 0.043774 0.000837 0.001179 0.017809 0.029949
None 0.000104 0.000876 0.002698 0.001010 0.000897 0.000732 0.001378 0.001251 0.000439 0.000774 0.002446 0.001114
Nuclear Periphery 0.000160 0.000151 0.005960 0.001182 0.000320 0.000172 0.002660 0.001130 0.000227 0.000166 0.005329 0.001160
Nuclear Periphery Foci 0.000079 0.000202 0.003160 0.002600 0.000221 0.000067 0.006365 0.001543 0.000139 0.000107 0.003773 0.002144
Nucleolus 0.000446 0.001128 0.005050 0.002253 0.001150 0.000844 0.007295 0.002443 0.000743 0.000927 0.005479 0.002335
Nucleus 0.006585 0.012699 0.243833* 0.029756 0.026383 0.012904 0.122398 0.038358 0.014947 0.012844 0.220603* 0.033470
Peroxisomes 0.000976 0.002823 0.003445 0.010780 0.002741 0.001317 0.007151 0.016175 0.001722 0.001758 0.004154 0.013109
Vacuole 0.840691* 0.636970* 0.218689* 0.463677* 0.631650* 0.793177* 0.402434* 0.339832* 0.752404* 0.747443* 0.253839* 0.410205*
Vacuole Periphery 0.012106 0.018165 0.002213 0.007276 0.007925 0.018359 0.002598 0.005144 0.010340 0.018302 0.002287 0.006356

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.56 -10.63 -4.03 -3.23 -1.12 -1.25 -11.03 -2.76 -2.48 -1.83 -1.72 -13.38 -3.91 -3.26 -2.20
Bud Neck -3.64 -9.58 -6.08 -1.25 3.57 1.63 -1.82 -2.26 -3.88 0.11 -1.54 -8.92 -6.00 -4.42 3.51
Bud Site -8.43 -3.67 -8.92 -1.70 -6.37 -5.22 -3.27 -8.37 -5.47 -4.12 -8.72 -4.77 -12.22 -5.96 -8.30
Cell Periphery 1.78 10.96 10.60 6.05 -0.83 -7.35 2.04 6.87 11.27 5.19 -1.42 11.71 12.00 12.57 1.61
Cytoplasm -12.57 -45.35 -21.83 -6.67 16.92 18.51 -2.95 -9.89 -25.73 -5.05 5.74 -34.63 -21.39 -26.48 11.34
Cytoplasmic Foci -11.29 -8.14 -14.40 0.69 -8.37 13.11 0.06 -1.46 -13.08 -1.33 1.76 -2.13 -10.62 -12.36 -9.06
Eisosomes -3.11 -6.53 -4.17 0.41 2.84 3.84 -7.21 1.94 -2.70 8.51 1.04 -7.18 -1.78 -2.11 6.23
Endoplasmic Reticulum 1.74 -9.46 1.85 0.00 15.50 -6.08 -9.01 1.49 7.74 10.24 -4.35 -13.95 2.36 7.01 19.61
Endosome -8.06 4.40 -13.80 -6.19 -16.24 1.90 -3.16 -3.39 -4.81 0.54 -4.07 3.61 -13.06 -10.09 -14.77
Golgi -2.41 2.44 -2.22 -1.17 -3.13 -2.51 2.83 -2.62 -1.71 -3.17 -3.07 3.54 -3.41 -2.02 -4.49
Lipid Particles -1.18 -6.98 -6.30 -5.51 0.96 5.55 -3.31 2.30 -4.73 5.81 3.58 -6.01 -3.53 -7.18 3.20
Mitochondria 0.44 0.29 0.25 -0.26 -0.00 -1.82 2.38 -2.07 -1.27 -3.13 -0.56 0.40 -1.23 -0.93 -1.99
Mitotic Spindle -1.14 -11.58 -8.05 -7.91 0.70 0.56 -2.88 -8.52 -8.79 -5.18 -0.57 -11.44 -11.70 -11.65 -2.29
None -1.35 -19.77 -2.94 -0.37 4.28 0.55 -1.90 -1.71 -2.65 0.72 -1.40 -13.17 -3.45 -1.53 5.31
Nuclear Periphery 0.21 -23.92 -11.63 -11.88 18.28 4.13 -9.81 -10.11 -11.27 4.91 2.49 -25.31 -15.41 -16.29 18.74
Nuclear Periphery Foci -5.68 -8.01 -4.93 -4.73 1.73 6.00 -6.37 -4.48 -4.92 4.74 2.16 -9.90 -6.30 -6.38 4.05
Nucleolus -2.38 -14.08 -8.05 -3.89 8.95 2.26 -6.03 -5.07 -6.90 4.60 -0.97 -14.85 -9.46 -8.24 10.18
Nucleus -3.65 -46.26 -14.43 -10.28 37.06 6.90 -13.55 -9.77 -14.33 8.85 1.74 -46.06 -16.73 -17.69 36.19
Peroxisomes -5.09 -5.59 -9.57 -6.88 -6.70 2.28 -2.76 -5.68 -7.58 -3.48 0.05 -4.17 -10.33 -10.95 -8.02
Vacuole 18.56 80.36* 38.92* 12.61 -25.26 -16.95 15.00 20.19 39.92* 1.35 0.60 67.73* 40.91 40.46* -21.60
Vacuole Periphery -4.87 18.28 8.61 10.47 -6.43 -11.06 11.13 8.13 17.38 -2.67 -10.44 21.52 11.53 18.74 -7.14
Standard name
Human Ortholog
Description Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (96%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Grx7

Grx7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Grx7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available