Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 5 | 2 | 2 | 4 | 3 | 0 | 9 | 5 | 3 | 2 | 0 | 58 | 55 | 25 | 1 | 2 | 0 | 2 | 1 | 3 | 0 | 8 | 3 |
Bud | 1 | 1 | 2 | 5 | 7 | 9 | 10 | 27 | 15 | 20 | 26 | 20 | 11 | 14 | 13 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 2 | 1 | 2 | 1 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 53 | 29 | 27 | 35 | 44 | 60 | 68 | 43 | 80 | 63 | 66 | 91 | 270 | 378 | 93 | 15 | 18 | 0 | 1 | 0 | 2 | 2 | 5 |
Endoplasmic Reticulum | 5 | 15 | 0 | 5 | 5 | 4 | 8 | 6 | 5 | 4 | 3 | 2 | 80 | 85 | 30 | 12 | 1 | 4 | 0 | 0 | 2 | 2 | 7 | 3 |
Endosome | 31 | 6 | 89 | 93 | 110 | 87 | 86 | 174 | 59 | 79 | 36 | 95 | 172 | 103 | 153 | 28 | 12 | 10 | 46 | 60 | 104 | 86 | 135 | 86 |
Golgi | 8 | 18 | 21 | 35 | 21 | 12 | 14 | 22 | 10 | 3 | 4 | 3 | 33 | 2 | 2 | 5 | 2 | 8 | 51 | 61 | 119 | 70 | 126 | 75 |
Mitochondria | 40 | 48 | 65 | 70 | 58 | 110 | 121 | 183 | 144 | 141 | 154 | 142 | 23 | 2 | 4 | 17 | 2 | 5 | 8 | 14 | 8 | 26 | 71 | 48 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 6 | 1 | 3 | 0 | 5 | 0 | 1 | 2 | 12 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 1 | 6 | 7 | 7 | 12 | 18 | 5 | 12 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 3 | 8 | 6 | 10 | 4 | 15 | 3 | 10 | 10 | 12 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 6 | 7 | 3 | 1 |
SpindlePole | 2 | 1 | 5 | 9 | 11 | 12 | 6 | 27 | 12 | 9 | 8 | 17 | 30 | 38 | 61 | 2 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 62 | 37 | 77 | 33 | 59 | 52 | 62 | 88 | 52 | 71 | 59 | 68 | 191 | 99 | 100 | 181 | 52 | 68 | 45 | 53 | 29 | 41 | 184 | 248 |
Unique Cell Count | 139 | 144 | 235 | 217 | 247 | 272 | 313 | 501 | 289 | 345 | 307 | 353 | 580 | 596 | 697 | 254 | 77 | 94 | 159 | 199 | 278 | 239 | 547 | 479 |
Labelled Cell Count | 182 | 185 | 296 | 295 | 327 | 355 | 387 | 646 | 357 | 437 | 383 | 440 | 692 | 678 | 779 | 354 | 93 | 117 | 159 | 199 | 278 | 239 | 547 | 479 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 6.7 | 6.8 | 7.5 | 7.5 | 7.0 | 6.8 | 6.8 | 6.7 | 6.3 | 6.5 | 6.4 | 7.0 | 6.2 | 6.0 | 6.4 | 6.8 | 9.0 | 7.6 | 7.5 | 7.5 |
Std Deviation (1e-4) | 0.9 | 1.1 | 1.4 | 1.5 | 2.0 | 1.5 | 1.4 | 1.4 | 1.3 | 1.2 | 1.2 | 1.0 | 0.7 | 0.6 | 0.7 | 1.4 | 1.5 | 2.3 | 1.3 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.14 | 0.03 | 0.0 | -0.01 | -0.03 | -0.12 | -0.07 | -0.1 | 0.03 | -0.14 | -0.18 | -0.09 | 0.0 | 0.4 | 0.16 | 0.14 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 4.3 | 2.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.9 | 1.9 | 3.0 | 2.8 | 3.1 | 4.0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.6 | 1.2 | 2.1 | 0.5 | 0.8 | 3.3 | 2.5 | 2.1 | 1.2 | 8.9 | 11.2 | 6.2 | 1.6 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.0 | 6.1 | 3.2 | 3.4 | 0 | 0 |
Endosome | 1.1 | 1.5 | -1.4 | -2.6 | -0.8 | -4.4 | -3.9 | -7.2 | -2.8 | -2.3 | -6.3 | -4.8 | -7.0 | -3.6 | -4.9 |
Golgi | 2.3 | -0.2 | -2.1 | -2.1 | -2.5 | -2.6 | -4.8 | -4.2 | -4.9 | -1.7 | -6.8 | -7.4 | -3.4 | -1.8 | -0.1 |
Mitochondria | 1.1 | -1.1 | 3.0 | 2.7 | 2.4 | 5.2 | 3.3 | 5.3 | 3.1 | -9.9 | -13.0 | -13.7 | -6.2 | -4.6 | -4.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.6 | 2.5 | 0 | 2.4 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.7 | 0 | 1.7 | 0 | 1.4 | 0 | 1.3 | 1.5 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.2 | 1.4 | 1.4 | 0 | 2.0 | 1.3 | 0.4 | 0.4 | 1.7 | 1.9 | 2.5 | 3.4 | 0 | 0 | 0 |
Vacuole | -4.3 | -2.2 | -3.5 | -3.5 | -4.6 | -3.9 | -3.3 | -3.6 | -3.7 | 0 | -5.1 | -6.2 | 8.5 | 5.4 | 6.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.754 | 4.4869 | 3.7545 | 3.3893 | 3.5147 | 4.2534 | 4.925 | 7.2072 | 7.1294 | 5.5307 | 6.8158 | 7.2505 | 5.4644 | 7.3352 | 6.3459 | 6.4333 | 7.0561 | 6.6537 |
Actin | 0.0187 | 0.0001 | 0.0003 | 0 | 0 | 0.0002 | 0.0086 | 0.0014 | 0.0006 | 0.0001 | 0 | 0.0005 | 0.0006 | 0.0003 | 0.0023 | 0.0001 | 0 | 0.0004 |
Bud | 0.0006 | 0.0002 | 0.0002 | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.002 | 0.0007 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0003 |
Bud Periphery | 0.0009 | 0.0003 | 0.0002 | 0.0013 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0042 | 0.0005 | 0.0003 | 0.0113 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Cell Periphery | 0.0004 | 0.0004 | 0.0001 | 0.0022 | 0.0001 | 0.0007 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0.0102 | 0.0168 | 0.0042 | 0.0043 | 0.0024 | 0.0093 | 0.0075 | 0.0045 | 0.0019 | 0.0031 | 0.0013 | 0.0046 | 0.0049 | 0.003 | 0.003 | 0.0024 | 0.0027 | 0.0025 |
Cytoplasmic Foci | 0.0273 | 0.0316 | 0.0332 | 0.019 | 0.0209 | 0.0298 | 0.0429 | 0.0467 | 0.0367 | 0.0514 | 0.0321 | 0.0218 | 0.0405 | 0.0501 | 0.0343 | 0.01 | 0.0246 | 0.0251 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0115 | 0.0051 | 0.002 | 0.0017 | 0.0012 | 0.0023 | 0.0055 | 0.0019 | 0.0035 | 0.0008 | 0.0007 | 0.0017 | 0.008 | 0.0019 | 0.0033 | 0.0023 | 0.0015 | 0.0038 |
Endosome | 0.5134 | 0.5547 | 0.6251 | 0.6308 | 0.5778 | 0.5412 | 0.5124 | 0.5353 | 0.4711 | 0.582 | 0.593 | 0.3957 | 0.5474 | 0.5069 | 0.5705 | 0.4584 | 0.574 | 0.5073 |
Golgi | 0.0919 | 0.0801 | 0.1504 | 0.0687 | 0.0746 | 0.147 | 0.333 | 0.2672 | 0.3871 | 0.2384 | 0.1787 | 0.3609 | 0.2263 | 0.2802 | 0.1954 | 0.1017 | 0.2198 | 0.2349 |
Lipid Particles | 0.0198 | 0.0065 | 0.0057 | 0.0027 | 0.0032 | 0.0098 | 0.0101 | 0.0103 | 0.0075 | 0.0116 | 0.0053 | 0.0241 | 0.0087 | 0.0141 | 0.0053 | 0.0037 | 0.0312 | 0.0089 |
Mitochondria | 0.0296 | 0.0088 | 0.0313 | 0.0031 | 0.0032 | 0.0165 | 0.0227 | 0.0441 | 0.0572 | 0.0157 | 0.0031 | 0.0207 | 0.0169 | 0.0084 | 0.0453 | 0.0127 | 0.0032 | 0.0346 |
None | 0.0092 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0155 | 0.0086 | 0.0001 | 0.0002 | 0 | 0.0033 | 0.012 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 |
Nuclear Periphery | 0.0309 | 0.0076 | 0.0029 | 0.0026 | 0.0016 | 0.0029 | 0.0045 | 0.0007 | 0.0024 | 0.001 | 0.0006 | 0.0031 | 0.0035 | 0.0015 | 0.0016 | 0.0024 | 0.0014 | 0.0045 |
Nucleolus | 0.0023 | 0.0003 | 0.0001 | 0.0027 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 |
Nucleus | 0.0131 | 0.0014 | 0.0005 | 0.0016 | 0.0005 | 0.0015 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0004 |
Peroxisomes | 0.0042 | 0.0008 | 0.001 | 0.0003 | 0.0004 | 0.0009 | 0.004 | 0.0035 | 0.0011 | 0.0015 | 0.0006 | 0.002 | 0.0016 | 0.0026 | 0.0121 | 0.0003 | 0.0004 | 0.0013 |
Punctate Nuclear | 0.0067 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0011 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Vacuole | 0.1263 | 0.2091 | 0.093 | 0.169 | 0.2305 | 0.1618 | 0.0151 | 0.0457 | 0.0145 | 0.046 | 0.1179 | 0.0883 | 0.0808 | 0.0755 | 0.0814 | 0.1891 | 0.0948 | 0.0944 |
Vacuole Periphery | 0.0764 | 0.0746 | 0.0487 | 0.076 | 0.0824 | 0.0731 | 0.0133 | 0.0267 | 0.0156 | 0.0462 | 0.0658 | 0.0718 | 0.047 | 0.0536 | 0.0444 | 0.2158 | 0.0446 | 0.0806 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 127.931 | 88.5209 | 44.8789 | 60.9485 | 84.5289 | 65.1595 | 81.7205 | 61.9096 | 60.7155 | 95.58 |
Translational Efficiency | 0.909 | 0.9289 | 1.1287 | 0.8656 | 0.9716 | 1.4623 | 0.8163 | 0.7935 | 0.8 | 0.7752 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|