Standard name
Human Ortholog
Description Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0.05 0.06 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.37 0.12 0.12 0.14 0.16 0.19 0.14 0.15 0.23 0.21 0.19 0.16 0.45 0.54 0.37 0.19 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.1 0 0 0 0 0 0 0 0 0 0 0.14 0.14 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.22 0 0.38 0.43 0.45 0.32 0.27 0.35 0.2 0.23 0.12 0.27 0.3 0.17 0.22 0.11 0.16 0.11 0.29 0.3 0.38 0.36 0.25 0.18
Golgi 0.06 0.12 0.09 0.16 0.09 0 0 0 0 0 0 0 0.06 0 0 0 0 0.09 0.32 0.31 0.43 0.3 0.23 0.16
Mitochondria 0.29 0.33 0.28 0.32 0.23 0.4 0.39 0.37 0.5 0.41 0.5 0.4 0 0 0 0.07 0 0.05 0.06 0.07 0 0.11 0.13 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.05 0.06 0.09 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.45 0.26 0.33 0.15 0.24 0.19 0.2 0.18 0.18 0.21 0.19 0.19 0.33 0.17 0.14 0.71 0.68 0.72 0.29 0.27 0.1 0.17 0.34 0.52
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 5 2 2 4 3 0 9 5 3 2 0 58 55 25 1 2 0 2 1 3 0 8 3
Bud 1 1 2 5 7 9 10 27 15 20 26 20 11 14 13 1 0 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 4 5 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 2 1 0 4 2 0 0 0 0 0 0
Cell Periphery 3 0 1 1 1 0 2 0 2 1 2 1 2 4 3 0 0 0 0 0 0 0 0 0
Cytoplasm 24 53 29 27 35 44 60 68 43 80 63 66 91 270 378 93 15 18 0 1 0 2 2 5
Endoplasmic Reticulum 5 15 0 5 5 4 8 6 5 4 3 2 80 85 30 12 1 4 0 0 2 2 7 3
Endosome 31 6 89 93 110 87 86 174 59 79 36 95 172 103 153 28 12 10 46 60 104 86 135 86
Golgi 8 18 21 35 21 12 14 22 10 3 4 3 33 2 2 5 2 8 51 61 119 70 126 75
Mitochondria 40 48 65 70 58 110 121 183 144 141 154 142 23 2 4 17 2 5 8 14 8 26 71 48
Nucleus 0 0 1 0 0 2 0 6 1 3 0 5 0 1 2 12 4 2 0 0 0 0 0 0
Nuclear Periphery 1 0 0 1 3 2 2 1 0 1 1 0 1 0 0 1 1 0 0 1 0 0 0 1
Nucleolus 1 0 1 6 7 7 12 18 5 12 11 7 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 3 8 6 10 4 15 3 10 10 12 0 1 3 1 0 0 0 1 6 7 3 1
SpindlePole 2 1 5 9 11 12 6 27 12 9 8 17 30 38 61 2 2 1 1 0 1 0 0 0
Vac/Vac Membrane 62 37 77 33 59 52 62 88 52 71 59 68 191 99 100 181 52 68 45 53 29 41 184 248
Unique Cell Count 139 144 235 217 247 272 313 501 289 345 307 353 580 596 697 254 77 94 159 199 278 239 547 479
Labelled Cell Count 182 185 296 295 327 355 387 646 357 437 383 440 692 678 779 354 93 117 159 199 278 239 547 479


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 6.7 6.8 7.5 7.5 7.0 6.8 6.8 6.7 6.3 6.5 6.4 7.0 6.2 6.0 6.4 6.8 9.0 7.6 7.5 7.5
Std Deviation (1e-4) 0.9 1.1 1.4 1.5 2.0 1.5 1.4 1.4 1.3 1.2 1.2 1.0 0.7 0.6 0.7 1.4 1.5 2.3 1.3 1.5 1.3
Intensity Change (Log2) 0.14 0.14 0.03 0.0 -0.01 -0.03 -0.12 -0.07 -0.1 0.03 -0.14 -0.18 -0.09 0.0 0.4 0.16 0.14 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 4.5 4.3 2.2 0 0 0
Bud 0 0 1.9 1.9 3.0 2.8 3.1 4.0 3.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.6 1.2 2.1 0.5 0.8 3.3 2.5 2.1 1.2 8.9 11.2 6.2 1.6 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 6.0 6.1 3.2 3.4 0 0
Endosome 1.1 1.5 -1.4 -2.6 -0.8 -4.4 -3.9 -7.2 -2.8 -2.3 -6.3 -4.8 -7.0 -3.6 -4.9
Golgi 2.3 -0.2 -2.1 -2.1 -2.5 -2.6 -4.8 -4.2 -4.9 -1.7 -6.8 -7.4 -3.4 -1.8 -0.1
Mitochondria 1.1 -1.1 3.0 2.7 2.4 5.2 3.3 5.3 3.1 -9.9 -13.0 -13.7 -6.2 -4.6 -4.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.6 2.5 0 2.4 2.5 0 0 0 0 0 0 0
Peroxisomes 1.7 0 1.7 0 1.4 0 1.3 1.5 1.6 0 0 0 0 0 0
SpindlePole 1.2 1.4 1.4 0 2.0 1.3 0.4 0.4 1.7 1.9 2.5 3.4 0 0 0
Vacuole -4.3 -2.2 -3.5 -3.5 -4.6 -3.9 -3.3 -3.6 -3.7 0 -5.1 -6.2 8.5 5.4 6.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.754 4.4869 3.7545 3.3893 3.5147 4.2534 4.925 7.2072 7.1294 5.5307 6.8158 7.2505 5.4644 7.3352 6.3459 6.4333 7.0561 6.6537
Actin 0.0187 0.0001 0.0003 0 0 0.0002 0.0086 0.0014 0.0006 0.0001 0 0.0005 0.0006 0.0003 0.0023 0.0001 0 0.0004
Bud 0.0006 0.0002 0.0002 0.0017 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.002 0.0007 0.0002 0.0007 0.0002 0.0005 0.0002 0.0005 0.0002 0.0004 0.0001 0.0002 0.0001 0.0004 0.0002 0.0001 0.0001 0.0003
Bud Periphery 0.0009 0.0003 0.0002 0.0013 0.0001 0.0003 0.0006 0.0004 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002
Bud Site 0.0042 0.0005 0.0003 0.0113 0.0003 0.0003 0.0002 0.0005 0.0001 0.0007 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002
Cell Periphery 0.0004 0.0004 0.0001 0.0022 0.0001 0.0007 0.0003 0.0009 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002
Cytoplasm 0.0102 0.0168 0.0042 0.0043 0.0024 0.0093 0.0075 0.0045 0.0019 0.0031 0.0013 0.0046 0.0049 0.003 0.003 0.0024 0.0027 0.0025
Cytoplasmic Foci 0.0273 0.0316 0.0332 0.019 0.0209 0.0298 0.0429 0.0467 0.0367 0.0514 0.0321 0.0218 0.0405 0.0501 0.0343 0.01 0.0246 0.0251
Eisosomes 0.0004 0 0 0 0 0 0.0016 0.0004 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0115 0.0051 0.002 0.0017 0.0012 0.0023 0.0055 0.0019 0.0035 0.0008 0.0007 0.0017 0.008 0.0019 0.0033 0.0023 0.0015 0.0038
Endosome 0.5134 0.5547 0.6251 0.6308 0.5778 0.5412 0.5124 0.5353 0.4711 0.582 0.593 0.3957 0.5474 0.5069 0.5705 0.4584 0.574 0.5073
Golgi 0.0919 0.0801 0.1504 0.0687 0.0746 0.147 0.333 0.2672 0.3871 0.2384 0.1787 0.3609 0.2263 0.2802 0.1954 0.1017 0.2198 0.2349
Lipid Particles 0.0198 0.0065 0.0057 0.0027 0.0032 0.0098 0.0101 0.0103 0.0075 0.0116 0.0053 0.0241 0.0087 0.0141 0.0053 0.0037 0.0312 0.0089
Mitochondria 0.0296 0.0088 0.0313 0.0031 0.0032 0.0165 0.0227 0.0441 0.0572 0.0157 0.0031 0.0207 0.0169 0.0084 0.0453 0.0127 0.0032 0.0346
None 0.0092 0.0002 0.0003 0.0001 0.0001 0.0004 0.0155 0.0086 0.0001 0.0002 0 0.0033 0.012 0.0001 0.0001 0.0001 0.0006 0.0001
Nuclear Periphery 0.0309 0.0076 0.0029 0.0026 0.0016 0.0029 0.0045 0.0007 0.0024 0.001 0.0006 0.0031 0.0035 0.0015 0.0016 0.0024 0.0014 0.0045
Nucleolus 0.0023 0.0003 0.0001 0.0027 0.0002 0.0008 0.0001 0.0002 0 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002
Nucleus 0.0131 0.0014 0.0005 0.0016 0.0005 0.0015 0.0006 0.0003 0.0001 0.0002 0.0002 0.0003 0.0005 0.0005 0.0002 0.0004 0.0003 0.0004
Peroxisomes 0.0042 0.0008 0.001 0.0003 0.0004 0.0009 0.004 0.0035 0.0011 0.0015 0.0006 0.002 0.0016 0.0026 0.0121 0.0003 0.0004 0.0013
Punctate Nuclear 0.0067 0.0001 0.0001 0.0001 0 0.0005 0.0011 0.0001 0 0.0002 0 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001
Vacuole 0.1263 0.2091 0.093 0.169 0.2305 0.1618 0.0151 0.0457 0.0145 0.046 0.1179 0.0883 0.0808 0.0755 0.0814 0.1891 0.0948 0.0944
Vacuole Periphery 0.0764 0.0746 0.0487 0.076 0.0824 0.0731 0.0133 0.0267 0.0156 0.0462 0.0658 0.0718 0.047 0.0536 0.0444 0.2158 0.0446 0.0806

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 127.931 88.5209 44.8789 60.9485 84.5289 65.1595 81.7205 61.9096 60.7155 95.58
Translational Efficiency 0.909 0.9289 1.1287 0.8656 0.9716 1.4623 0.8163 0.7935 0.8 0.7752

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
Localization
Cell Percentages Golgi (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mnn2

Mnn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mnn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available