ORF
Human Ortholog
Description Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.4 0.31 – 0.39 0.31 0.32 – 0.27 0.21 0.22 0.14 0.15 0.27 0.27 0.18 0.17 0.06 – 0 0.06 0 0 0.07 0.1
Bud 0.1 0.07 – 0.06 0 0.09 – 0.1 0.06 0.08 0.09 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0.1 0.09 0.09 0.08 0.08
Bud Site 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0.74 0.69 – 0.73 0.55 0.45 – 0.48 0.44 0.44 0.44 0.45 0.71 0.72 0.75 0.79 0.76 – 0.87 0.72 0.67 0.61 0.61 0.57
Cytoplasm 0.12 0.14 – 0.17 0.19 0.25 – 0.29 0.26 0.19 0.24 0.26 0.29 0.28 0.21 0.12 0.29 – 0 0 0.06 0.11 0.07 0.07
Endoplasmic Reticulum 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0.05 0.05 0
Endosome 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.13 0.08 – 0.55 0.36 0.5 – 0.53 0.61 0.67 0.66 0.65 0.12 0.14 0.2 0.21 0.06 – 0 0 0.06 0 0 0
Nucleus 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0.08 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 103 41 – 41 40 38 – 58 22 55 24 30 39 41 32 4 1 – 8 9 0 1 11 26
Bud 26 9 – 6 5 11 – 21 6 21 15 8 6 5 3 1 0 – 1 3 0 0 1 8
Bud Neck 2 0 – 0 1 0 – 0 0 0 0 0 0 0 0 0 0 – 13 16 4 3 12 21
Bud Site 5 3 – 1 5 4 – 1 1 2 0 1 0 2 1 0 0 – – – – – – –
Cell Periphery 189 91 – 76 71 53 – 105 47 112 76 90 102 109 135 19 13 – 291 112 35 24 96 144
Cytoplasm 30 18 – 18 25 30 – 64 28 47 42 53 41 42 38 3 5 – 3 4 3 4 11 17
Endoplasmic Reticulum 1 0 – 1 3 4 – 5 3 5 4 2 4 3 4 0 1 – 3 3 1 2 7 10
Endosome 0 0 – 0 1 0 – 0 0 0 0 0 0 0 0 0 0 – 2 0 0 0 3 2
Golgi 1 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 5
Mitochondria 33 11 – 57 46 60 – 115 65 168 114 131 17 21 36 5 1 – 3 1 3 0 4 4
Nucleus 2 2 – 0 0 1 – 1 1 1 5 0 0 0 2 1 0 – 0 0 0 0 1 1
Nuclear Periphery 0 0 – 0 0 0 – 2 0 0 1 0 1 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 – 1 1 1 1 2 0 1 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 – 2 5 5 – 5 0 1 0 1 0 0 5 0 0 – 0 0 0 0 0 1
SpindlePole 2 2 – 0 2 3 – 1 1 2 0 0 2 3 1 0 0 – 0 0 0 0 0 2
Vac/Vac Membrane 0 0 – 0 2 1 – 1 1 2 5 4 0 0 1 0 0 – 5 1 3 3 4 3
Unique Cell Count 255 132 104 129 119 218 107 252 173 201 143 152 181 24 17 336 157 53 40 159 255
Labelled Cell Count 394 177 202 206 210 380 176 417 287 322 212 227 258 33 21 336 157 53 40 159 255


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.6 – 6.5 7.6 6.2 – 6.3 5.6 5.9 5.9 5.8 9.3 9.2 9.1 13.6 13.7 – – 9.3 9.3
Std Deviation (1e-4) 1.0 1.1 – 1.2 1.8 1.1 – 1.5 1.4 1.0 1.8 1.7 2.0 2.5 2.3 3.2 2.7 – – 3.3 2.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6474 7.1281 6.2757 5.5384 5.1675 6.2866 6.6908 7.1167 6.5066 6.0443 5.9322 6.3172 9.2948 9.9027 9.1996 9.6812 9.1927 9.3239
Actin 0.0334 0.0001 0.0089 0.0133 0.0035 0.0104 0.0381 0.0003 0.0161 0 0.0043 0.0049 0.0773 0.0027 0.0141 0.0001 0.0234 0.0048
Bud 0.0015 0.0003 0.0002 0.0011 0.0002 0.0008 0.0009 0.0003 0.0011 0.0003 0.0002 0.0005 0.0011 0.0003 0.0008 0.0007 0.0005 0.0005
Bud Neck 0.0052 0.0025 0.0069 0.0056 0.0006 0.0286 0.0021 0.0027 0.0036 0.0009 0.0011 0.0156 0.0018 0.0028 0.0061 0.001 0.0013 0.0341
Bud Periphery 0.0084 0.0018 0.0047 0.0121 0.0063 0.0086 0.0044 0.0025 0.0126 0.0118 0.003 0.0089 0.009 0.0011 0.006 0.0256 0.0082 0.0094
Bud Site 0.0273 0.0972 0.0106 0.0021 0.0009 0.0012 0.0036 0.0917 0.026 0.0009 0.0011 0.0008 0.0041 0.1145 0.0449 0.0007 0.0022 0.0027
Cell Periphery 0.756 0.823 0.8556 0.8486 0.8846 0.9006 0.7914 0.8523 0.8463 0.954 0.8326 0.8787 0.742 0.7972 0.8272 0.9357 0.8009 0.9221
Cytoplasm 0.0304 0.0671 0.0483 0.016 0.0231 0.0155 0.0255 0.0417 0.0275 0.0181 0.027 0.0067 0.0289 0.0656 0.045 0.0145 0.0118 0.012
Cytoplasmic Foci 0.0158 0 0.0016 0.0156 0.0079 0.0027 0.0175 0 0.0092 0 0.0066 0.0035 0.0107 0.0002 0.0043 0 0.0012 0.0001
Eisosomes 0.0112 0.0003 0.0039 0.0004 0.0004 0.0005 0.0022 0.0006 0.0023 0.0002 0.0002 0.0009 0.0028 0.0005 0.0004 0.0001 0.0298 0.0011
Endoplasmic Reticulum 0.0093 0.0014 0.0059 0.0083 0.013 0.003 0.0156 0.0015 0.004 0.0055 0.0052 0.003 0.0142 0.0054 0.0049 0.0078 0.013 0.0055
Endosome 0.0252 0 0.0024 0.0183 0.0044 0.0035 0.0341 0 0.0094 0 0.0581 0.0207 0.019 0.0002 0.0074 0 0.0025 0
Golgi 0.0122 0 0.0041 0.0076 0.0018 0.0022 0.0117 0 0.0086 0 0.0057 0.0111 0.0128 0.0002 0.0058 0 0.0034 0.0001
Lipid Particles 0.0118 0.0001 0.0024 0.0114 0.0172 0.0022 0.0075 0.0001 0.003 0.0001 0.0162 0.0033 0.0295 0.0021 0.0053 0.0002 0.0033 0.0001
Mitochondria 0.0086 0 0.0153 0.0163 0.0016 0.0028 0.0155 0 0.0179 0 0.0038 0.0264 0.0037 0 0.0121 0 0.0042 0.0003
None 0.0095 0.0003 0.0184 0.0024 0.0053 0.003 0.0011 0.0001 0.0003 0.0001 0.0011 0.0002 0.0026 0.0001 0.0006 0.0001 0.0397 0.0001
Nuclear Periphery 0.0049 0.0003 0.0014 0.0019 0.0008 0.0007 0.0037 0.0003 0.0005 0.0003 0.0175 0.0022 0.0102 0.0006 0.0009 0.0004 0.009 0.0002
Nucleolus 0.0008 0.0001 0.0001 0.0003 0.0005 0.0003 0.0001 0 0.0001 0 0.0003 0.0001 0.0003 0.0001 0.0002 0 0.0043 0.0001
Nucleus 0.0018 0.0002 0.0004 0.0005 0.0004 0.0005 0.0006 0.0002 0.0004 0.0001 0.0009 0.0004 0.001 0.0003 0.0005 0.0001 0.0058 0.0002
Peroxisomes 0.0112 0 0.0011 0.0041 0.0071 0.0025 0.0079 0 0.0022 0 0.0021 0.0014 0.0082 0 0.0024 0 0.0007 0
Punctate Nuclear 0.0049 0 0.0006 0.005 0.002 0.0039 0.0063 0 0.0001 0 0.0033 0.0001 0.0048 0 0.001 0 0.0278 0
Vacuole 0.0095 0.0051 0.0073 0.0083 0.0181 0.0064 0.009 0.0054 0.0083 0.0076 0.0072 0.0082 0.0142 0.0061 0.0095 0.0128 0.0065 0.0066
Vacuole Periphery 0.0011 0 0.0002 0.0011 0.0004 0.0001 0.0011 0 0.0003 0.0001 0.0024 0.0024 0.0018 0 0.0003 0.0001 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.6188 45.878 47.2825 68.2647 64.6918 79.7887 79.2447 87.8369 58.5611 76.3977
Translational Efficiency 3.8629 3.6762 2.9442 1.3724 2.7229 3.2755 2.4701 2.416 3.674 2.7336

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
923 1250 1526 156 1352 1829 997 1429 2275 3079 2523 1585

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 863.58 990.59 1092.04 1132.03 1018.83 945.90 1119.39 1064.74 955.84 964.04 1102.85 1071.36
Standard Deviation 121.63 137.31 135.88 146.02 158.09 130.94 132.78 148.66 163.29 135.35 135.32 149.75
Intensity Change Log 2 — 0.197958 0.338624 0.390510 — -0.107154 0.135799 0.063588 — 0.040863 0.232399 0.222803

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003407 0.007558 0.029450 0.074388 0.001007 0.004045 0.006706 0.008745 0.001981 0.005472 0.020463 0.015206
Bud Neck 0.034668 0.034845 0.076055 0.062856 0.003959 0.024757 0.032893 0.031523 0.016418 0.028853 0.058999 0.034607
Bud Site 0.048108 0.080422 0.142050* 0.200454* 0.016879 0.039274 0.073459 0.076583 0.029549 0.055979 0.114945* 0.088775
Cell Periphery 0.721206* 0.601395* 0.307792* 0.400900* 0.944374* 0.680321* 0.737583* 0.670211* 0.853831* 0.648279* 0.477630* 0.643704*
Cytoplasm 0.165718* 0.244914* 0.256951* 0.154202* 0.019161 0.219737* 0.115534* 0.186404* 0.078621 0.229958* 0.201068* 0.183235*
Cytoplasmic Foci 0.001790 0.003632 0.006332 0.005578 0.002047 0.005112 0.000808 0.001465 0.001943 0.004511 0.004149 0.001870
Eisosomes 0.004602 0.004012 0.027121 0.019427 0.000190 0.002804 0.005850 0.003834 0.001980 0.003295 0.018715 0.005368
Endoplasmic Reticulum 0.004859 0.004578 0.062354 0.011027 0.006529 0.005916 0.007625 0.002289 0.005851 0.005373 0.040727 0.003149
Endosome 0.000558 0.001474 0.001749 0.003961 0.000886 0.001366 0.000383 0.002024 0.000753 0.001410 0.001209 0.002214
Golgi 0.000680 0.002655 0.002617 0.001829 0.000790 0.002633 0.000610 0.001948 0.000746 0.002642 0.001824 0.001936
Lipid Particles 0.000308 0.001514 0.007170 0.008032 0.000377 0.001158 0.000977 0.001170 0.000349 0.001302 0.004723 0.001845
Mitochondria 0.002973 0.001990 0.000551 0.000086 0.000485 0.002500 0.000220 0.003054 0.001494 0.002293 0.000420 0.002762
Mitotic Spindle 0.000161 0.000189 0.000416 0.000240 0.000179 0.000483 0.000403 0.000262 0.000172 0.000364 0.000411 0.000260
None 0.001495 0.000032 0.000312 0.000023 0.000013 0.000040 0.000036 0.000128 0.000614 0.000037 0.000203 0.000118
Nuclear Periphery 0.000125 0.000116 0.000300 0.000068 0.000199 0.000142 0.000292 0.000118 0.000169 0.000131 0.000297 0.000113
Nuclear Periphery Foci 0.000157 0.000247 0.002619 0.000917 0.000118 0.000176 0.000627 0.000236 0.000134 0.000205 0.001831 0.000303
Nucleolus 0.000029 0.000062 0.000053 0.000011 0.000022 0.000045 0.000022 0.000025 0.000025 0.000052 0.000041 0.000024
Nucleus 0.002967 0.000879 0.021984 0.008720 0.000115 0.000545 0.001710 0.001046 0.001272 0.000681 0.013972 0.001801
Peroxisomes 0.002476 0.002275 0.001330 0.001073 0.000373 0.004969 0.000727 0.000566 0.001226 0.003875 0.001092 0.000616
Vacuole 0.003260 0.006253 0.052634 0.046131 0.001272 0.003525 0.013352 0.007376 0.002078 0.004633 0.037111 0.011190
Vacuole Periphery 0.000453 0.000956 0.000161 0.000077 0.001024 0.000450 0.000182 0.000995 0.000793 0.000655 0.000169 0.000905

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.74 -17.46 -6.29 -5.91 -3.95 -5.39 -8.67 -8.25 -4.75 -1.95 -6.21 -19.53 -8.97 -6.39 3.10
Bud Neck -0.05 -8.47 -2.57 -2.60 1.22 -11.10 -11.15 -12.31 -2.47 0.42 -6.08 -15.11 -6.98 -2.18 7.46
Bud Site -5.22 -15.73 -8.02 -6.26 -3.03 -6.88 -11.54 -13.63 -7.93 -0.53 -8.25 -22.20 -12.98 -6.85 5.02
Cell Periphery 8.61 35.75* 11.16 7.04 -3.26 33.36* 23.16 30.70* 0.96 5.92 26.61 45.67 22.12 0.50 -16.23
Cytoplasm -7.90 -10.20 0.58 5.18 6.09 -35.83* -17.90 -26.79 4.16 -9.01 -27.69 -22.71 -16.05 6.56 2.51
Cytoplasmic Foci -2.44 -8.35 -2.35 -1.25 0.35 -2.83 1.75 0.76 4.14 -2.03 -3.59 -4.38 0.13 4.06 5.48
Eisosomes 0.65 -18.45 -5.11 -5.42 2.60 -10.81 -14.17 -10.66 -2.48 3.85 -3.31 -23.72 -6.41 -4.20 17.45
Endoplasmic Reticulum 0.20 -18.69 -2.10 -2.27 12.78 0.50 -0.97 4.33 3.91 6.73 0.52 -17.78 3.21 2.96 19.91
Endosome -2.67 -7.71 -1.06 -0.77 -0.69 -1.73 2.67 -1.78 -1.02 -2.68 -3.04 -3.24 -2.27 -1.22 -1.56
Golgi -3.43 -12.20 -1.09 0.70 0.74 -2.65 0.46 -1.55 0.80 -2.08 -3.99 -4.38 -1.89 0.99 -0.20
Lipid Particles -1.65 -15.70 -2.25 -1.86 -0.24 -2.08 -1.62 -1.22 -0.02 -0.31 -2.57 -13.19 -2.30 -0.77 4.18
Mitochondria 0.81 2.14 2.55 4.21 15.40* -4.84 3.63 -3.05 -0.59 -3.37 -1.45 2.33 -1.43 -0.57 -3.09
Mitotic Spindle -0.75 -2.82 -1.04 -0.62 1.56 -1.05 -12.21 -1.90 0.76 3.08 -1.11 -4.41 -2.17 0.60 2.28
None 1.34 1.09 1.35 1.44 9.76 -5.07 -6.11 -1.26 -0.96 -1.01 1.30 0.93 1.11 -0.98 1.02
Nuclear Periphery 0.85 -12.24 5.37 4.23 16.52 3.07 -3.54 4.06 2.14 8.03 3.15 -8.39 4.05 1.84 13.57
Nuclear Periphery Foci -2.98 -21.96 -5.13 -4.48 8.90 -4.09 -18.05 -7.03 -3.40 13.12 -4.77 -23.74 -8.04 -4.13 20.66
Nucleolus -1.32 -6.44 5.33 2.07 17.45 -2.20 0.05 -1.92 1.89 -1.97 -2.27 -8.38 0.70 2.38 9.92
Nucleus 1.63 -11.37 -2.11 -3.34 5.10 -8.54 -12.20 -13.12 -7.24 4.78 1.17 -15.21 -0.81 -4.49 18.08
Peroxisomes 0.40 3.63 3.54 2.73 0.93 -9.12 -3.92 -2.70 8.71 1.61 -7.41 0.99 4.29 9.62 5.86
Vacuole -4.43 -25.20 -5.57 -5.19 0.63 -10.19 -17.97 -14.97 -9.30 7.89 -8.35 -27.43 -10.03 -7.04 16.60
Vacuole Periphery -1.83 6.22 7.91 3.23 5.93 4.83 7.37 0.08 -1.61 -2.41 1.03 8.83 -0.36 -0.77 -2.42
ORF
Human Ortholog
Description Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (95%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

YBR016W

YBR016W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr016w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available