Standard name | |
---|---|
Human Ortholog | |
Description | Endoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 1 | 1 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 2 | 8 | 12 | 20 | 4 | 0 | 3 | 4 | 0 | 1 | 1 | 1 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 5 | 4 | 7 | 1 | 7 | 1 | 4 | 4 | 0 | 0 | 0 | 4 | 9 | 2 | 4 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 1 | 7 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 155 | 447 | 78 | 143 | 178 | 247 | 321 | 452 | 346 | 429 | 377 | 430 | 155 | 205 | 280 | 82 | 52 | 51 | 10 | 28 | 7 | 8 | 7 | 5 |
Endoplasmic Reticulum | 24 | 142 | 13 | 9 | 8 | 15 | 15 | 12 | 10 | 14 | 13 | 11 | 263 | 307 | 233 | 16 | 9 | 11 | 101 | 281 | 65 | 122 | 121 | 135 |
Endosome | 2 | 5 | 10 | 1 | 5 | 7 | 5 | 5 | 9 | 1 | 1 | 7 | 13 | 33 | 39 | 6 | 6 | 9 | 12 | 27 | 15 | 11 | 19 | 20 |
Golgi | 10 | 29 | 13 | 3 | 1 | 0 | 2 | 7 | 1 | 5 | 3 | 0 | 16 | 6 | 6 | 21 | 15 | 11 | 6 | 8 | 9 | 11 | 14 | 14 |
Mitochondria | 3 | 0 | 3 | 0 | 4 | 7 | 13 | 13 | 7 | 8 | 9 | 17 | 0 | 0 | 0 | 6 | 3 | 3 | 5 | 2 | 2 | 2 | 1 | 2 |
Nucleus | 8 | 3 | 14 | 4 | 8 | 32 | 42 | 35 | 21 | 20 | 10 | 9 | 1 | 3 | 0 | 117 | 56 | 50 | 3 | 1 | 2 | 4 | 1 | 1 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 15 | 10 | 5 | 7 | 7 | 5 |
Nucleolus | 37 | 2 | 0 | 0 | 2 | 4 | 1 | 4 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 6 | 1 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 198 | 536 | 253 | 137 | 266 | 452 | 403 | 608 | 309 | 334 | 249 | 263 | 117 | 116 | 84 | 370 | 242 | 236 | 342 | 757 | 248 | 374 | 330 | 339 |
Unique Cell Count | 388 | 1011 | 345 | 241 | 404 | 651 | 675 | 966 | 597 | 689 | 542 | 625 | 496 | 614 | 587 | 521 | 339 | 327 | 514 | 1139 | 367 | 554 | 518 | 540 |
Labelled Cell Count | 442 | 1167 | 388 | 298 | 473 | 767 | 805 | 1144 | 709 | 822 | 665 | 752 | 580 | 687 | 673 | 626 | 384 | 381 | 514 | 1139 | 367 | 554 | 518 | 540 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.7 | 18.4 | 13.3 | 13.9 | 14.0 | 12.7 | 11.3 | 12.2 | 12.3 | 12.0 | 11.3 | 11.7 | 16.1 | 13.9 | 12.2 | 17.5 | 15.8 | 14.1 | 20.0 | 19.6 | 20.8 |
Std Deviation (1e-4) | 6.4 | 3.7 | 4.0 | 3.3 | 3.8 | 3.9 | 4.2 | 3.9 | 4.2 | 3.8 | 3.7 | 4.1 | 3.2 | 2.7 | 2.4 | 5.8 | 5.6 | 5.4 | 4.7 | 5.0 | 4.7 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.08 | -0.06 | -0.23 | -0.12 | -0.11 | -0.15 | -0.23 | -0.18 | 0.28 | 0.07 | -0.12 | 0.4 | 0.26 | 0.09 | 0.59 | 0.56 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9.0 | 6.2 | 4.9 | 7.7 | 7.9 | 10.5 | 12.0 | 13.6 | 13.8 | 2.8 | 3.5 | 7.6 | -2.6 | -2.4 | -2.3 |
Endoplasmic Reticulum | 0 | -1.5 | -1.3 | -1.4 | -2.9 | -2.0 | -1.7 | -1.2 | -1.9 | 15.0 | 14.6 | 12.0 | -0.6 | -0.8 | -0.3 |
Endosome | 0 | -1.6 | -2.1 | -2.7 | 0 | -1.5 | 0 | 0 | -2.0 | -0.2 | 1.8 | 2.5 | -1.9 | -1.0 | -0.1 |
Golgi | -1.8 | -3.5 | 0 | -4.4 | -4.0 | -4.4 | -3.5 | -3.5 | 0 | -0.4 | -3.0 | -2.9 | 0.2 | 0.4 | -0.3 |
Mitochondria | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.7 | -1.7 | 0.6 | 1.4 | -0.4 | -0.4 | -1.0 | -2.0 | -2.6 | -4.2 | -4.0 | -4.9 | 7.4 | 5.4 | 5.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.2 | -2.2 | -1.3 | -4.3 | -3.5 | -6.5 | -7.6 | -8.0 | -9.3 | -14.3 | -16.6 | -18.1 | -0.7 | -0.6 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.2969 | 11.3953 | 9.7719 | 10.2031 | 8.454 | 10.3499 | 13.6974 | 17.7025 | 15.0178 | 13.9321 | 13.9888 | 15.3495 | 13.3784 | 16.2725 | 14.7703 | 14.2465 | 14.9613 | 15.5727 |
Actin | 0.001 | 0.0001 | 0.0015 | 0.0001 | 0.0005 | 0.0006 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0092 | 0.0002 | 0.0002 | 0 | 0.0011 | 0.0001 |
Bud | 0.0012 | 0.0027 | 0.0044 | 0.0004 | 0.0007 | 0.001 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0004 |
Bud Neck | 0.0012 | 0.0015 | 0.0015 | 0.0014 | 0.0016 | 0.0021 | 0.0004 | 0.0016 | 0.0028 | 0.0012 | 0.0029 | 0.0013 | 0.0007 | 0.0027 | 0.001 | 0.0011 | 0.0025 | 0.0018 |
Bud Periphery | 0.0013 | 0.0013 | 0.0023 | 0.0016 | 0.0015 | 0.0033 | 0.0009 | 0.0009 | 0.0023 | 0.0022 | 0.003 | 0.002 | 0.001 | 0.0011 | 0.001 | 0.0013 | 0.0014 | 0.0016 |
Bud Site | 0.0045 | 0.0049 | 0.0059 | 0.0012 | 0.0032 | 0.0019 | 0.0004 | 0.0004 | 0.0018 | 0.0009 | 0.0005 | 0.001 | 0.0006 | 0.0013 | 0.0008 | 0.0013 | 0.0006 | 0.0004 |
Cell Periphery | 0.009 | 0.0117 | 0.0099 | 0.0176 | 0.0045 | 0.008 | 0.0256 | 0.0314 | 0.0212 | 0.0267 | 0.0357 | 0.0158 | 0.0065 | 0.0222 | 0.0187 | 0.021 | 0.0251 | 0.0095 |
Cytoplasm | 0.0413 | 0.07 | 0.0355 | 0.0374 | 0.03 | 0.0287 | 0.0188 | 0.0068 | 0.0219 | 0.0107 | 0.0084 | 0.0144 | 0.0316 | 0.0848 | 0.0436 | 0.0294 | 0.0626 | 0.0346 |
Cytoplasmic Foci | 0.0234 | 0.0047 | 0.0183 | 0.0051 | 0.0243 | 0.0097 | 0.0021 | 0.0003 | 0.0024 | 0.0006 | 0.0005 | 0.0013 | 0.0081 | 0.0027 | 0.0032 | 0.0104 | 0.0023 | 0.0032 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.2702 | 0.2453 | 0.2367 | 0.1462 | 0.2275 | 0.2392 | 0.4411 | 0.4229 | 0.3762 | 0.3441 | 0.2885 | 0.3737 | 0.4544 | 0.3063 | 0.3367 | 0.1187 | 0.2494 | 0.3252 |
Endosome | 0.1451 | 0.0584 | 0.1339 | 0.1164 | 0.1238 | 0.0781 | 0.0516 | 0.0155 | 0.0513 | 0.0213 | 0.0182 | 0.0335 | 0.061 | 0.0311 | 0.0596 | 0.0653 | 0.0246 | 0.0418 |
Golgi | 0.0095 | 0.0014 | 0.0071 | 0.0028 | 0.0065 | 0.0063 | 0.0023 | 0.0007 | 0.0066 | 0.0006 | 0.0008 | 0.0009 | 0.004 | 0.001 | 0.0019 | 0.0013 | 0.0008 | 0.0013 |
Lipid Particles | 0.0287 | 0.0115 | 0.0125 | 0.0061 | 0.0484 | 0.0071 | 0.0125 | 0.0043 | 0.0161 | 0.0078 | 0.0121 | 0.0087 | 0.0136 | 0.0104 | 0.0087 | 0.0051 | 0.0081 | 0.0059 |
Mitochondria | 0.0154 | 0.0022 | 0.0053 | 0.0027 | 0.0109 | 0.0058 | 0.0022 | 0.0002 | 0.0023 | 0.0003 | 0.0017 | 0.0009 | 0.003 | 0.001 | 0.0016 | 0.0008 | 0.0007 | 0.0012 |
None | 0.0036 | 0.0005 | 0.002 | 0.0002 | 0.0007 | 0.0015 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.003 | 0.0007 | 0.0007 | 0.0002 | 0.0015 | 0.0003 |
Nuclear Periphery | 0.064 | 0.0332 | 0.0383 | 0.0209 | 0.0338 | 0.0514 | 0.044 | 0.0229 | 0.0319 | 0.0339 | 0.0379 | 0.0379 | 0.0764 | 0.0475 | 0.053 | 0.02 | 0.0351 | 0.0749 |
Nucleolus | 0.0068 | 0.0044 | 0.0052 | 0.0015 | 0.0123 | 0.002 | 0.0005 | 0.0017 | 0.0006 | 0.0003 | 0.0006 | 0.0005 | 0.0032 | 0.0031 | 0.0013 | 0.001 | 0.0009 | 0.0008 |
Nucleus | 0.0129 | 0.0119 | 0.0099 | 0.0045 | 0.007 | 0.0113 | 0.005 | 0.0025 | 0.0026 | 0.0029 | 0.0054 | 0.0039 | 0.0209 | 0.0176 | 0.0085 | 0.0054 | 0.008 | 0.0255 |
Peroxisomes | 0.0036 | 0.0002 | 0.0025 | 0.0001 | 0.0105 | 0.0013 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0031 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Punctate Nuclear | 0.0086 | 0.0026 | 0.0039 | 0.0002 | 0.001 | 0.0015 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0061 | 0.0017 | 0.0006 | 0.0003 | 0.0054 | 0.0008 |
Vacuole | 0.3186 | 0.5111 | 0.433 | 0.6015 | 0.4215 | 0.5087 | 0.3673 | 0.4699 | 0.4321 | 0.5304 | 0.5532 | 0.4738 | 0.271 | 0.4425 | 0.4241 | 0.6651 | 0.5538 | 0.4297 |
Vacuole Periphery | 0.0301 | 0.0205 | 0.0303 | 0.032 | 0.0299 | 0.0304 | 0.0235 | 0.0176 | 0.0269 | 0.0154 | 0.03 | 0.0297 | 0.0219 | 0.0218 | 0.0344 | 0.0516 | 0.0158 | 0.0408 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.5675 | 73.5982 | 83.7004 | 80.5189 | 75.4175 | 81.3903 | 91.4011 | 88.44 | 105.7234 | 81.8325 |
Translational Efficiency | 0.9994 | 1.0575 | 0.9147 | 1.0595 | 0.8565 | 1.1239 | 0.9512 | 0.9807 | 0.8614 | 0.8473 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2191 | 1419 | 271 | 978 | 1635 | 1730 | 1947 | 1387 | 3826 | 3149 | 2218 | 2365 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1160.63 | 1379.14 | 1525.50 | 1303.44 | 1210.69 | 1353.42 | 1586.26 | 1636.22 | 1182.02 | 1365.01 | 1578.84 | 1498.61 |
Standard Deviation | 182.20 | 261.67 | 209.78 | 316.47 | 259.47 | 412.95 | 243.64 | 318.94 | 219.98 | 353.13 | 240.58 | 357.68 |
Intensity Change Log 2 | — | 0.248861 | 0.394374 | 0.167416 | — | 0.160780 | 0.389800 | 0.434537 | — | 0.204563 | 0.392040 | 0.309959 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000013 | 0.000079 | 0.000145 | 0.000845 | 0.000043 | 0.000058 | 0.000406 | 0.000601 | 0.000026 | 0.000067 | 0.000374 | 0.000702 |
Bud Neck | 0.000463 | 0.000798 | 0.003602 | 0.005819 | 0.000845 | 0.000696 | 0.000841 | 0.002686 | 0.000626 | 0.000742 | 0.001179 | 0.003982 |
Bud Site | 0.000526 | 0.002440 | 0.002266 | 0.021120 | 0.002662 | 0.001478 | 0.002880 | 0.011661 | 0.001438 | 0.001912 | 0.002805 | 0.015573 |
Cell Periphery | 0.005571 | 0.008454 | 0.001217 | 0.001017 | 0.004219 | 0.003838 | 0.003389 | 0.007725 | 0.004993 | 0.005918 | 0.003124 | 0.004951 |
Cytoplasm | 0.001046 | 0.007210 | 0.008172 | 0.048776 | 0.005703 | 0.002123 | 0.017997 | 0.021860 | 0.003036 | 0.004415 | 0.016797 | 0.032991 |
Cytoplasmic Foci | 0.000635 | 0.001759 | 0.006240 | 0.024188 | 0.003832 | 0.001779 | 0.003807 | 0.011364 | 0.002002 | 0.001770 | 0.004105 | 0.016667 |
Eisosomes | 0.000031 | 0.000010 | 0.000014 | 0.000020 | 0.000006 | 0.000007 | 0.000036 | 0.000036 | 0.000020 | 0.000008 | 0.000034 | 0.000029 |
Endoplasmic Reticulum | 0.154519* | 0.409383* | 0.492773* | 0.045309 | 0.142465* | 0.258684* | 0.806792* | 0.348950* | 0.149368* | 0.326592* | 0.768424* | 0.223385* |
Endosome | 0.006668 | 0.014997 | 0.048961 | 0.079866 | 0.020485 | 0.011077 | 0.031123 | 0.061786 | 0.012573 | 0.012844 | 0.033302 | 0.069263 |
Golgi | 0.000377 | 0.001480 | 0.003484 | 0.016252 | 0.002595 | 0.001879 | 0.005884 | 0.017837 | 0.001325 | 0.001699 | 0.005591 | 0.017181 |
Lipid Particles | 0.000487 | 0.001035 | 0.016848 | 0.016039 | 0.000942 | 0.000727 | 0.021576 | 0.032744 | 0.000681 | 0.000866 | 0.020998 | 0.025836 |
Mitochondria | 0.000219 | 0.000728 | 0.000078 | 0.004486 | 0.000216 | 0.000488 | 0.000550 | 0.002127 | 0.000218 | 0.000596 | 0.000492 | 0.003102 |
Mitotic Spindle | 0.000011 | 0.000729 | 0.000949 | 0.014702 | 0.000027 | 0.000580 | 0.000654 | 0.002237 | 0.000018 | 0.000647 | 0.000690 | 0.007392 |
None | 0.000013 | 0.000243 | 0.000007 | 0.002244 | 0.000717 | 0.001780 | 0.000946 | 0.000822 | 0.000314 | 0.001087 | 0.000831 | 0.001410 |
Nuclear Periphery | 0.001836 | 0.001260 | 0.024778 | 0.004949 | 0.002819 | 0.003398 | 0.001535 | 0.000932 | 0.002256 | 0.002435 | 0.004375 | 0.002593 |
Nuclear Periphery Foci | 0.000999 | 0.006542 | 0.087080 | 0.033082 | 0.007730 | 0.002419 | 0.014816 | 0.019959 | 0.003876 | 0.004277 | 0.023645 | 0.025386 |
Nucleolus | 0.000244 | 0.000164 | 0.000049 | 0.002117 | 0.000624 | 0.000442 | 0.000049 | 0.000153 | 0.000406 | 0.000317 | 0.000049 | 0.000965 |
Nucleus | 0.000241 | 0.000542 | 0.000731 | 0.005138 | 0.000737 | 0.001989 | 0.000690 | 0.000893 | 0.000453 | 0.001337 | 0.000695 | 0.002648 |
Peroxisomes | 0.000039 | 0.000047 | 0.000067 | 0.001103 | 0.000060 | 0.000104 | 0.000220 | 0.000254 | 0.000048 | 0.000079 | 0.000201 | 0.000605 |
Vacuole | 0.782889* | 0.518956* | 0.294360* | 0.625809* | 0.691264* | 0.679175* | 0.082008 | 0.425965* | 0.743734* | 0.606977* | 0.107953 | 0.508607* |
Vacuole Periphery | 0.043174 | 0.023144 | 0.008181 | 0.047121 | 0.112007* | 0.027278 | 0.003801 | 0.029407 | 0.072589 | 0.025415 | 0.004336 | 0.036732 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.94 | -4.37 | -2.94 | -2.71 | -2.46 | -1.29 | -1.99 | -2.85 | -2.77 | -0.71 | -5.13 | -2.17 | -4.13 | -3.87 | -1.40 |
Bud Neck | -3.14 | -6.32 | -12.34 | -11.41 | -3.65 | 0.79 | -0.38 | -5.40 | -5.61 | -5.28 | -1.21 | -5.10 | -12.43 | -11.66 | -9.80 |
Bud Site | -4.98 | -3.64 | -9.90 | -8.88 | -8.87 | 1.76 | -0.75 | -7.24 | -7.94 | -6.86 | -1.34 | -3.60 | -12.57 | -11.87 | -10.79 |
Cell Periphery | -3.25 | 7.37 | 8.07 | 10.76 | 0.93 | 0.76 | 1.61 | -4.79 | -5.81 | -6.38 | -1.82 | 4.10 | 0.25 | 2.11 | -3.90 |
Cytoplasm | -5.59 | -4.45 | -12.48 | -10.80 | -10.22 | 3.79 | -8.76 | -8.09 | -10.11 | -1.79 | -2.22 | -12.00 | -15.15 | -14.27 | -7.79 |
Cytoplasmic Foci | -3.38 | -3.40 | -13.63 | -12.92 | -7.48 | 2.92 | -0.13 | -6.99 | -8.87 | -7.19 | 0.53 | -4.74 | -15.49 | -15.48 | -12.47 |
Eisosomes | 0.93 | 0.76 | 0.51 | -4.31 | -2.27 | -1.59 | -8.99 | -11.39 | -10.38 | 0.29 | 0.91 | -1.05 | -0.67 | -11.01 | 1.48 |
Endoplasmic Reticulum | -25.05* | -15.57 | 18.17 | 35.97* | 20.75 | -13.65 | -75.77* | -18.10 | -7.12 | 38.50* | -26.82 | -79.70 | -9.25 | 12.28 | 56.49* |
Endosome | -8.98 | -9.25 | -19.38 | -16.74 | -4.63 | 6.87 | -5.80 | -16.15 | -20.36 | -10.54 | -0.41 | -13.09 | -26.50 | -26.07 | -13.36 |
Golgi | -2.29 | -2.17 | -8.40 | -7.51 | -5.17 | 0.96 | -3.97 | -10.36 | -10.22 | -7.31 | -0.86 | -6.09 | -13.76 | -12.72 | -8.44 |
Lipid Particles | -3.15 | -3.81 | -7.91 | -7.63 | 0.12 | 1.14 | -11.56 | -12.56 | -12.62 | -3.43 | -1.49 | -12.29 | -14.81 | -14.68 | -1.93 |
Mitochondria | -1.21 | 1.03 | -4.27 | -3.45 | -4.48 | -1.68 | -5.39 | -2.90 | -2.40 | -2.36 | -1.77 | -2.96 | -5.05 | -4.11 | -4.56 |
Mitotic Spindle | -1.18 | -2.51 | -7.01 | -6.49 | -6.47 | -1.20 | -2.80 | -4.24 | -2.38 | -2.69 | -1.68 | -3.33 | -7.91 | -6.84 | -7.02 |
None | -2.05 | 1.41 | -3.14 | -2.74 | -3.15 | -1.25 | -0.37 | -0.20 | 1.13 | 0.21 | -1.70 | -1.11 | -2.79 | -0.44 | -0.92 |
Nuclear Periphery | 1.80 | -6.39 | -4.10 | -4.97 | 5.36 | -0.77 | 1.95 | 3.22 | 4.98 | 2.10 | -0.45 | -3.57 | -1.00 | -0.60 | 2.77 |
Nuclear Periphery Foci | -5.62 | -9.82 | -10.03 | -8.05 | 5.57 | 4.42 | -4.37 | -6.21 | -9.82 | -2.41 | -0.64 | -11.50 | -12.47 | -12.21 | -1.02 |
Nucleolus | 3.03 | 8.29 | -2.46 | -2.57 | -2.72 | 1.18 | 6.11 | 4.90 | 2.49 | -4.84 | 1.13 | 8.49 | -1.76 | -2.01 | -2.90 |
Nucleus | -2.23 | -2.64 | -7.64 | -7.11 | -6.73 | -1.87 | -0.02 | -1.09 | 1.50 | -0.73 | -2.37 | -1.25 | -7.69 | -3.25 | -5.67 |
Peroxisomes | -0.45 | -1.19 | -4.61 | -4.58 | -4.48 | -0.93 | -4.47 | -5.91 | -2.35 | -0.66 | -1.09 | -4.72 | -5.73 | -5.16 | -3.89 |
Vacuole | 26.64* | 26.58 | 15.14 | -7.79 | -16.09 | 1.36 | 83.91* | 24.48 | 22.31 | -34.81 | 20.40 | 108.33* | 30.14 | 11.08 | -48.29 |
Vacuole Periphery | 8.83 | 16.95 | -0.20 | -6.95 | -12.65 | 22.03* | 29.81* | 21.08 | -0.86 | -15.52* | 21.83 | 34.61 | 15.54 | -5.64 | -20.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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