Standard name
Human Ortholog
Description Endoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.44 0.23 0.59 0.44 0.38 0.48 0.47 0.58 0.62 0.7 0.69 0.31 0.33 0.48 0.16 0.15 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.14 0 0 0 0 0 0 0 0 0 0 0.53 0.5 0.4 0 0 0 0.2 0.25 0.18 0.22 0.23 0.25
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0.22 0.17 0.15 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.53 0.73 0.57 0.66 0.69 0.6 0.63 0.52 0.48 0.46 0.42 0.24 0.19 0.14 0.71 0.71 0.72 0.67 0.66 0.68 0.68 0.64 0.63
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 1 1 1 0 1 2 1 1 0 2 8 12 20 4 0 3 4 0 1 1 1 1
Bud 0 0 1 0 0 1 2 5 4 7 1 7 1 4 4 0 0 0 4 9 2 4 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 1 0 0 1 0 0 0 1 1 3 5 1 7 3 0 0 0 0 0 0 0 0
Cytoplasm 155 447 78 143 178 247 321 452 346 429 377 430 155 205 280 82 52 51 10 28 7 8 7 5
Endoplasmic Reticulum 24 142 13 9 8 15 15 12 10 14 13 11 263 307 233 16 9 11 101 281 65 122 121 135
Endosome 2 5 10 1 5 7 5 5 9 1 1 7 13 33 39 6 6 9 12 27 15 11 19 20
Golgi 10 29 13 3 1 0 2 7 1 5 3 0 16 6 6 21 15 11 6 8 9 11 14 14
Mitochondria 3 0 3 0 4 7 13 13 7 8 9 17 0 0 0 6 3 3 5 2 2 2 1 2
Nucleus 8 3 14 4 8 32 42 35 21 20 10 9 1 3 0 117 56 50 3 1 2 4 1 1
Nuclear Periphery 1 0 1 0 0 1 0 0 0 1 0 1 1 0 0 0 0 0 15 10 5 7 7 5
Nucleolus 37 2 0 0 2 4 1 4 1 1 1 2 0 0 0 1 0 6 1 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 1 0 1 0 0
Vac/Vac Membrane 198 536 253 137 266 452 403 608 309 334 249 263 117 116 84 370 242 236 342 757 248 374 330 339
Unique Cell Count 388 1011 345 241 404 651 675 966 597 689 542 625 496 614 587 521 339 327 514 1139 367 554 518 540
Labelled Cell Count 442 1167 388 298 473 767 805 1144 709 822 665 752 580 687 673 626 384 381 514 1139 367 554 518 540


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.7 18.4 13.3 13.9 14.0 12.7 11.3 12.2 12.3 12.0 11.3 11.7 16.1 13.9 12.2 17.5 15.8 14.1 20.0 19.6 20.8
Std Deviation (1e-4) 6.4 3.7 4.0 3.3 3.8 3.9 4.2 3.9 4.2 3.8 3.7 4.1 3.2 2.7 2.4 5.8 5.6 5.4 4.7 5.0 4.7
Intensity Change (Log2) – – – 0.07 0.08 -0.06 -0.23 -0.12 -0.11 -0.15 -0.23 -0.18 0.28 0.07 -0.12 0.4 0.26 0.09 0.59 0.56 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9.0 6.2 4.9 7.7 7.9 10.5 12.0 13.6 13.8 2.8 3.5 7.6 -2.6 -2.4 -2.3
Endoplasmic Reticulum 0 -1.5 -1.3 -1.4 -2.9 -2.0 -1.7 -1.2 -1.9 15.0 14.6 12.0 -0.6 -0.8 -0.3
Endosome 0 -1.6 -2.1 -2.7 0 -1.5 0 0 -2.0 -0.2 1.8 2.5 -1.9 -1.0 -0.1
Golgi -1.8 -3.5 0 -4.4 -4.0 -4.4 -3.5 -3.5 0 -0.4 -3.0 -2.9 0.2 0.4 -0.3
Mitochondria 0 0 0 1.3 0 0 0 0 1.9 0 0 0 0 0 0
Nucleus -1.7 -1.7 0.6 1.4 -0.4 -0.4 -1.0 -2.0 -2.6 -4.2 -4.0 -4.9 7.4 5.4 5.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.2 -2.2 -1.3 -4.3 -3.5 -6.5 -7.6 -8.0 -9.3 -14.3 -16.6 -18.1 -0.7 -0.6 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.2969 11.3953 9.7719 10.2031 8.454 10.3499 13.6974 17.7025 15.0178 13.9321 13.9888 15.3495 13.3784 16.2725 14.7703 14.2465 14.9613 15.5727
Actin 0.001 0.0001 0.0015 0.0001 0.0005 0.0006 0.0004 0 0.0003 0.0001 0.0001 0.0001 0.0092 0.0002 0.0002 0 0.0011 0.0001
Bud 0.0012 0.0027 0.0044 0.0004 0.0007 0.001 0.0001 0.0002 0.0005 0.0004 0.0004 0.0004 0.0004 0.0004 0.0004 0.0004 0.0003 0.0004
Bud Neck 0.0012 0.0015 0.0015 0.0014 0.0016 0.0021 0.0004 0.0016 0.0028 0.0012 0.0029 0.0013 0.0007 0.0027 0.001 0.0011 0.0025 0.0018
Bud Periphery 0.0013 0.0013 0.0023 0.0016 0.0015 0.0033 0.0009 0.0009 0.0023 0.0022 0.003 0.002 0.001 0.0011 0.001 0.0013 0.0014 0.0016
Bud Site 0.0045 0.0049 0.0059 0.0012 0.0032 0.0019 0.0004 0.0004 0.0018 0.0009 0.0005 0.001 0.0006 0.0013 0.0008 0.0013 0.0006 0.0004
Cell Periphery 0.009 0.0117 0.0099 0.0176 0.0045 0.008 0.0256 0.0314 0.0212 0.0267 0.0357 0.0158 0.0065 0.0222 0.0187 0.021 0.0251 0.0095
Cytoplasm 0.0413 0.07 0.0355 0.0374 0.03 0.0287 0.0188 0.0068 0.0219 0.0107 0.0084 0.0144 0.0316 0.0848 0.0436 0.0294 0.0626 0.0346
Cytoplasmic Foci 0.0234 0.0047 0.0183 0.0051 0.0243 0.0097 0.0021 0.0003 0.0024 0.0006 0.0005 0.0013 0.0081 0.0027 0.0032 0.0104 0.0023 0.0032
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.2702 0.2453 0.2367 0.1462 0.2275 0.2392 0.4411 0.4229 0.3762 0.3441 0.2885 0.3737 0.4544 0.3063 0.3367 0.1187 0.2494 0.3252
Endosome 0.1451 0.0584 0.1339 0.1164 0.1238 0.0781 0.0516 0.0155 0.0513 0.0213 0.0182 0.0335 0.061 0.0311 0.0596 0.0653 0.0246 0.0418
Golgi 0.0095 0.0014 0.0071 0.0028 0.0065 0.0063 0.0023 0.0007 0.0066 0.0006 0.0008 0.0009 0.004 0.001 0.0019 0.0013 0.0008 0.0013
Lipid Particles 0.0287 0.0115 0.0125 0.0061 0.0484 0.0071 0.0125 0.0043 0.0161 0.0078 0.0121 0.0087 0.0136 0.0104 0.0087 0.0051 0.0081 0.0059
Mitochondria 0.0154 0.0022 0.0053 0.0027 0.0109 0.0058 0.0022 0.0002 0.0023 0.0003 0.0017 0.0009 0.003 0.001 0.0016 0.0008 0.0007 0.0012
None 0.0036 0.0005 0.002 0.0002 0.0007 0.0015 0.0004 0 0.0002 0.0001 0.0001 0.0002 0.003 0.0007 0.0007 0.0002 0.0015 0.0003
Nuclear Periphery 0.064 0.0332 0.0383 0.0209 0.0338 0.0514 0.044 0.0229 0.0319 0.0339 0.0379 0.0379 0.0764 0.0475 0.053 0.02 0.0351 0.0749
Nucleolus 0.0068 0.0044 0.0052 0.0015 0.0123 0.002 0.0005 0.0017 0.0006 0.0003 0.0006 0.0005 0.0032 0.0031 0.0013 0.001 0.0009 0.0008
Nucleus 0.0129 0.0119 0.0099 0.0045 0.007 0.0113 0.005 0.0025 0.0026 0.0029 0.0054 0.0039 0.0209 0.0176 0.0085 0.0054 0.008 0.0255
Peroxisomes 0.0036 0.0002 0.0025 0.0001 0.0105 0.0013 0.0001 0 0.0001 0 0 0 0.0031 0 0.0001 0.0001 0.0002 0
Punctate Nuclear 0.0086 0.0026 0.0039 0.0002 0.001 0.0015 0.0007 0 0.0001 0.0001 0.0001 0.0001 0.0061 0.0017 0.0006 0.0003 0.0054 0.0008
Vacuole 0.3186 0.5111 0.433 0.6015 0.4215 0.5087 0.3673 0.4699 0.4321 0.5304 0.5532 0.4738 0.271 0.4425 0.4241 0.6651 0.5538 0.4297
Vacuole Periphery 0.0301 0.0205 0.0303 0.032 0.0299 0.0304 0.0235 0.0176 0.0269 0.0154 0.03 0.0297 0.0219 0.0218 0.0344 0.0516 0.0158 0.0408

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.5675 73.5982 83.7004 80.5189 75.4175 81.3903 91.4011 88.44 105.7234 81.8325
Translational Efficiency 0.9994 1.0575 0.9147 1.0595 0.8565 1.1239 0.9512 0.9807 0.8614 0.8473

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Endoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages vacuole (31%), ER (12%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Csg2

Csg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Csg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available