Standard name
Human Ortholog
Description Ser/Thr protein kinase; phosphorylates Pan1p, Sla1p and Ent1p to negatively regulate endocytosis in response to membrane stress; regulates actin cytoskeleton organization and clathrin-dependent endocytosis; phosphorylated and inhibited by upstream kinase, Fpk1p; member, along with Ark1p and Prk1p, of the Ark kinase family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0.08 0 0.08 0.07 0 0.05 0 0.06 0.08 0.06 0.09 0.22 0.27 0.21 0.12 0.13 0.16 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.98 0.95 0.91 0.88 0.84 0.79 0.82 0.81 0.79 0.99 1.0 1.0 0.88 0.9 0.87 0.96 0.95 0.92 0.93 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.15 0.17 0.22 0.33 0.31 0.33 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 1 1 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 1 9 6 6 3 9 10 5 19 11 7 0 0 4 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 28 13 16 22 13 21 17 21 34 18 32 34 58 50 17 16 22 0 0 0 0 0 0
Cytoplasm 125 352 302 196 280 287 367 417 288 339 230 285 155 213 239 121 108 118 124 350 298 147 212 182
Endoplasmic Reticulum 0 3 1 1 1 2 3 2 1 5 0 0 1 0 1 3 2 3 0 1 4 3 4 4
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 1 0 0 0
Mitochondria 0 2 3 6 21 47 72 108 121 126 93 128 1 0 0 1 1 1 0 3 6 2 0 2
Nucleus 5 0 0 1 0 0 2 3 2 2 0 2 0 1 1 1 4 10 3 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 1 0
Nucleolus 0 0 1 0 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 4 17 1 0 2 6 0 0 0 8 3 6 1 7 4 1 0 0
Unique Cell Count 129 358 306 200 296 314 415 498 363 413 283 359 156 214 239 138 120 136 129 367 326 159 226 197
Labelled Cell Count 136 386 331 226 330 354 482 577 442 528 355 463 191 273 295 154 135 161 129 367 326 159 226 197


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 7.8 5.7 5.6 5.6 5.2 4.7 4.7 4.4 4.2 4.1 4.1 9.3 9.2 8.2 15.1 16.4 16.0 8.6 9.5 10.2
Std Deviation (1e-4) 1.2 1.1 0.7 0.9 0.9 1.3 1.0 1.0 1.3 1.1 1.1 1.1 1.5 1.2 1.3 11.7 3.4 2.8 1.4 1.6 1.7
Intensity Change (Log2) – – – -0.02 -0.04 -0.13 -0.27 -0.27 -0.38 -0.45 -0.48 -0.47 0.7 0.69 0.51 1.4 1.51 1.48 0.59 0.73 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -0.7 -1.8 0 0 0 0 0 0 0 0 -1.0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 1.7 -0.1 0 0 0 0 0 0 5.9 7.5 6.0 3.1 3.3 4.3
Cytoplasm -0.6 -2.8 -4.2 -5.3 -6.7 -7.7 -7.1 -7.1 -7.7 0.7 1.0 1.8 -5.1 -4.2 -5.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.8 6.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7971 7.1346 6.2606 5.9336 5.4198 6.3147 5.5159 6.6694 6.0976 5.3078 5.5961 6.4361 8.9525 10.0688 8.9686 9.9931 8.672 9.4327
Actin 0.2086 0.094 0.191 0.1897 0.2586 0.0897 0.0963 0.0768 0.1151 0.1474 0.1347 0.0729 0.103 0.2028 0.2971 0.3028 0.3734 0.1481
Bud 0.0027 0.0013 0.0021 0.0011 0.0007 0.0012 0.0048 0.0123 0.0097 0.0026 0.0148 0.0087 0.001 0.0006 0.0007 0.0009 0.0003 0.0005
Bud Neck 0.0252 0.0065 0.056 0.059 0.0237 0.2022 0.0102 0.0065 0.0462 0.011 0.0231 0.1237 0.0307 0.0085 0.0437 0.0076 0.0101 0.173
Bud Periphery 0.0046 0.0011 0.0034 0.0022 0.002 0.0019 0.0056 0.0092 0.0128 0.0036 0.0264 0.0171 0.0019 0.0005 0.001 0.0018 0.0004 0.0013
Bud Site 0.0202 0.0905 0.0557 0.0034 0.0015 0.0014 0.0354 0.167 0.0842 0.0072 0.0112 0.0035 0.0373 0.1286 0.0419 0.0112 0.0022 0.0014
Cell Periphery 0.0239 0.0116 0.013 0.0114 0.0072 0.0055 0.0184 0.0103 0.0122 0.0038 0.0155 0.0058 0.0207 0.0191 0.0255 0.0058 0.0045 0.0089
Cytoplasm 0.4557 0.7519 0.5974 0.6271 0.546 0.6232 0.7058 0.6681 0.644 0.692 0.6515 0.7086 0.7825 0.6073 0.5452 0.3432 0.5185 0.6384
Cytoplasmic Foci 0.0398 0.0145 0.0163 0.0328 0.0145 0.0233 0.0179 0.015 0.0163 0.04 0.0346 0.0144 0.0039 0.0073 0.0111 0.019 0.0178 0.0114
Eisosomes 0.003 0.0005 0.0009 0.0006 0.0006 0.0005 0.0019 0.0003 0.0014 0.0012 0.0013 0.0002 0.0008 0.0009 0.0026 0.0032 0.0019 0.0004
Endoplasmic Reticulum 0.0191 0.0062 0.0088 0.0087 0.0086 0.0136 0.0209 0.006 0.0114 0.0146 0.0125 0.0139 0.0103 0.0124 0.0118 0.0375 0.0034 0.0049
Endosome 0.0406 0.0078 0.015 0.0178 0.0132 0.0082 0.0108 0.0039 0.0089 0.0168 0.0149 0.007 0.0006 0.0009 0.0012 0.0105 0.0015 0.0022
Golgi 0.0353 0.0063 0.0188 0.0161 0.0118 0.0094 0.0112 0.01 0.0139 0.0205 0.0149 0.0067 0.0036 0.0073 0.014 0.0325 0.0087 0.0045
Lipid Particles 0.0249 0.0009 0.0055 0.01 0.026 0.0014 0.004 0.0012 0.0021 0.0091 0.0064 0.0044 0.0006 0.0008 0.0017 0.0183 0.0017 0.0005
Mitochondria 0.0187 0.0002 0.0034 0.0015 0.0079 0.0077 0.0036 0.0007 0.0016 0.0121 0.0021 0.0005 0.0001 0.0001 0.0001 0.0017 0.0002 0.0002
None 0.018 0.004 0.0019 0.0055 0.0018 0.0018 0.0187 0.0069 0.0045 0.0099 0.0061 0.0038 0.0013 0.0009 0.0008 0.0987 0.0319 0.0026
Nuclear Periphery 0.0136 0.0004 0.0024 0.0013 0.0219 0.0008 0.0153 0.0007 0.0039 0.001 0.0164 0.0024 0.0004 0.0003 0.0002 0.0023 0.0011 0.0001
Nucleolus 0.001 0 0.0005 0.0004 0.0012 0.0001 0.001 0.0004 0.0004 0.0003 0.0007 0.0003 0 0 0 0.0002 0.0015 0
Nucleus 0.0053 0.0005 0.0013 0.0011 0.0223 0.0005 0.0083 0.0018 0.0065 0.0009 0.0029 0.0015 0.0004 0.0002 0.0002 0.0005 0.0028 0.0002
Peroxisomes 0.0214 0.0008 0.0029 0.0068 0.025 0.0057 0.0022 0.0009 0.0013 0.0031 0.0024 0.0014 0.0002 0.0012 0.0008 0.1001 0.0024 0.0007
Punctate Nuclear 0.0127 0.0002 0.0015 0.0018 0.0012 0.0007 0.0019 0.0007 0.0017 0.0013 0.0018 0.0011 0.0001 0.0002 0.0002 0.0011 0.0154 0.0002
Vacuole 0.0039 0.0007 0.0016 0.0014 0.0023 0.0008 0.0049 0.0014 0.0017 0.0014 0.0043 0.0019 0.0003 0.0001 0.0001 0.0009 0.0001 0.0002
Vacuole Periphery 0.0017 0.0001 0.0005 0.0003 0.0021 0.0004 0.001 0.0001 0.0003 0.0004 0.0014 0.0002 0 0 0 0.0003 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.3259 18.678 32.3397 49.4613 36.5501 30.495 34.9978 44.3086 45.7294 39.4081
Translational Efficiency 1.0413 1.2407 1.0623 0.7828 0.7846 1.146 0.7906 0.7161 0.8431 0.8014

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ser/Thr protein kinase; phosphorylates Pan1p, Sla1p and Ent1p to negatively regulate endocytosis in response to membrane stress; regulates actin cytoskeleton organization and clathrin-dependent endocytosis; phosphorylated and inhibited by upstream kinase, Fpk1p; member, along with Ark1p and Prk1p, of the Ark kinase family
Localization
Cell Percentages cytoplasm (67%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Akl1

Akl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Akl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available