Standard name
Human Ortholog
Description Pre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0.05 0.05 0.05 0 0 0 0 0.06 0.13 0.17 0.06 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0 0 0 0 0.18 0.37 0.27 0.43 0.38 0.58 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.95 0.91 0.91 0.87 0.81 0.82 0.79 0.8 0.8 0.83 0.93 0.86 0.8 0.86 0.83 0.83 0.9 0.91 0.87 0.9 0.73 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.11 0 0 0.06 0.05 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 1 3
Bud 0 0 0 0 1 0 3 4 5 6 11 6 0 0 0 0 1 1 0 1 1 3 8 7
Bud Neck 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 3 4 16 7 18 10 16 10 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 8 24 32 21 32 31 26 31 18 14 43 107 126 25 9 28 2 4 1 2 4 2
Endoplasmic Reticulum 1 1 1 0 0 0 0 0 0 0 0 0 0 1 1 13 5 10 0 0 0 0 1 3
Endosome 0 0 0 0 3 0 0 2 0 1 0 0 2 1 10 2 2 2 0 2 2 2 6 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 1 0 0 1 0 2 3
Mitochondria 28 3 6 11 12 71 227 153 350 296 428 211 2 1 1 8 3 12 2 2 0 2 8 16
Nucleus 152 250 206 350 517 351 495 469 645 628 590 611 634 727 610 352 125 298 149 247 192 400 488 447
Nuclear Periphery 2 0 0 0 3 1 5 5 6 10 5 3 3 5 4 1 0 1 0 0 0 0 2 5
Nucleolus 1 1 0 0 0 0 5 2 5 7 12 6 1 2 3 3 1 1 1 0 2 3 21 33
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 3 2 1 6 1 4 1 14 2 4 1 3 2 2 0 1 1 4 11 9
Vac/Vac Membrane 3 8 1 4 15 16 14 7 22 23 6 24 5 11 21 35 17 40 5 8 12 22 107 133
Unique Cell Count 162 263 217 383 571 403 609 570 813 786 740 734 683 850 758 411 150 358 166 271 220 445 669 687
Labelled Cell Count 189 269 222 390 591 466 798 686 1078 1016 1088 901 695 859 780 443 166 396 166 271 220 445 669 687


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 6.2 6.2 6.0 5.8 4.9 4.5 4.8 4.3 4.5 4.1 4.7 6.9 6.7 6.6 7.1 7.9 7.4 5.9 6.3 6.6
Std Deviation (1e-4) 0.7 0.9 1.2 1.7 1.7 1.6 1.4 1.2 1.0 1.1 1.0 0.9 1.3 1.4 1.6 1.4 1.6 1.6 1.0 1.3 1.6
Intensity Change (Log2) – – – -0.03 -0.08 -0.34 -0.47 -0.37 -0.54 -0.46 -0.59 -0.39 0.15 0.12 0.1 0.21 0.35 0.25 -0.06 0.03 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 1.1 0.9 0.9 1.0 -0.4 0.2 -1.0 -1.5 1.4 3.8 4.9 1.3 1.0 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.4
Nucleus -1.6 -2.0 -3.1 -4.8 -4.5 -5.4 -5.2 -5.3 -4.3 -1.1 -3.7 -5.1 -3.5 -3.7 -4.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.6 0 0 0 2.1 0 2.3 0 0 0 4.1 4.7 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.544 3.7469 3.3001 2.4716 2.9523 3.3612 2.1313 2.6608 2.0633 1.8815 1.6808 2.1027 1.7806 1.7936 1.6778 1.9148 0.8834 1.3561
Actin 0 0 0 0 0 0 0.0151 0 0.0011 0 0 0.0001 0.0308 0 0.0315 0.003 0 0.0007
Bud 0 0 0 0 0 0 0.0002 0 0.0001 0 0 0 0.0015 0 0.0007 0.0002 0 0.0001
Bud Neck 0 0 0 0 0.0001 0.0002 0.0009 0.0001 0.0003 0 0.0002 0.0006 0.0014 0 0.0004 0.0006 0.0001 0.0005
Bud Periphery 0 0 0 0 0 0 0.0004 0 0.0003 0 0 0 0.0041 0.0001 0.0008 0.0002 0 0.0005
Bud Site 0 0 0 0 0 0 0.0011 0 0.0002 0 0 0.0001 0.0169 0.0001 0.0061 0.0031 0 0.0001
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0006 0 0.0001 0.0005 0 0.0002
Cytoplasm 0 0.0001 0.0001 0.0001 0.0003 0 0.0027 0.0001 0.0004 0 0.0001 0.0011 0.0014 0.0001 0.0014 0.0076 0 0.0001
Cytoplasmic Foci 0 0 0.0003 0 0 0 0.0153 0 0.0011 0 0 0.0003 0.0129 0 0.0021 0.0005 0 0.0001
Eisosomes 0 0 0 0 0 0 0.0002 0 0 0 0 0 0.0006 0 0.0001 0.0008 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.002 0 0.0015 0 0 0.0002 0.0033 0 0.0011 0.0029 0 0.0005
Endosome 0 0 0 0 0.0001 0 0.0085 0 0.0035 0 0 0.0006 0.0113 0 0.0053 0.0028 0 0.002
Golgi 0 0 0 0 0 0 0.0027 0 0.0008 0 0 0.0001 0.004 0 0.0019 0.0003 0 0.0003
Lipid Particles 0 0 0 0 0 0 0.0123 0 0.0031 0 0 0.0008 0.0182 0 0.0012 0.0005 0 0.001
Mitochondria 0.0002 0 0 0 0.0001 0 0.0046 0.0002 0.0011 0.0001 0.0003 0.0002 0.0266 0.0004 0.0027 0.002 0.0002 0.0041
None 0 0 0.0001 0.0001 0.0018 0 0.0038 0 0.0002 0 0 0.0013 0.0019 0 0.0003 0.0353 0 0.0002
Nuclear Periphery 0.0005 0.0034 0.0019 0.0008 0.0028 0.0027 0.013 0.0075 0.0066 0.0036 0.0023 0.0045 0.0189 0.0015 0.0067 0.031 0.0005 0.0194
Nucleolus 0.0078 0.009 0.0111 0.0091 0.0378 0.0329 0.0086 0.0282 0.0109 0.0109 0.0319 0.0295 0.01 0.0122 0.006 0.0071 0.015 0.021
Nucleus 0.9868 0.9734 0.9793 0.9892 0.9419 0.9576 0.8743 0.9628 0.9655 0.9848 0.9635 0.9578 0.8106 0.9848 0.9278 0.8958 0.9839 0.9442
Peroxisomes 0 0 0 0 0 0 0.0135 0 0.0011 0 0 0.0001 0.0112 0 0.0013 0.0001 0 0.0001
Punctate Nuclear 0.0046 0.014 0.0071 0.0006 0.0149 0.0063 0.018 0.0006 0.0008 0.0004 0.0015 0.0025 0.0048 0.0001 0.0014 0.0032 0.0001 0.0012
Vacuole 0 0 0 0 0 0 0.0018 0.0002 0.0007 0 0 0.0002 0.0056 0.0002 0.0005 0.0018 0 0.0013
Vacuole Periphery 0 0 0 0 0 0 0.0011 0.0002 0.0005 0 0.0001 0.0002 0.0035 0.0002 0.0003 0.0008 0 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6113 6.3056 11.3634 15.6618 12.4117 16.1139 13.2066 17.2124 15.4024 14.8933
Translational Efficiency 0.9746 1.9497 1.0445 0.8459 1.0886 0.8659 1.0685 0.7251 1.0111 1.1026

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
122 1081 2066 1707 1426 1988 172 191 1548 3069 2238 1898

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 766.15 872.28 848.29 968.17 695.34 825.29 947.96 1091.56 700.92 841.84 855.95 980.59
Standard Deviation 112.60 117.31 107.30 218.36 104.83 111.82 116.98 164.36 107.17 115.97 111.29 216.75
Intensity Change Log 2 — 0.187164 0.146931 0.337634 — 0.247183 0.447108 0.650601 — 0.216031 0.297548 0.495015

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000521 0.000302 0.000262 0.000702 0.000113 0.000186 0.000331 0.001322 0.000145 0.000227 0.000267 0.000764
Bud Neck 0.012810 0.003723 0.008058 0.015611 0.001441 0.005474 0.001495 0.014851 0.002337 0.004857 0.007554 0.015535
Bud Site 0.011877 0.000616 0.001062 0.002890 0.000381 0.000871 0.000343 0.006801 0.001287 0.000781 0.001007 0.003284
Cell Periphery 0.000128 0.000103 0.000065 0.000100 0.000134 0.000104 0.000051 0.000135 0.000133 0.000104 0.000064 0.000104
Cytoplasm 0.002774 0.002844 0.002388 0.007033 0.015026 0.004487 0.004423 0.010532 0.014060 0.003908 0.002545 0.007385
Cytoplasmic Foci 0.001509 0.001347 0.000123 0.001224 0.001910 0.001191 0.000199 0.001931 0.001878 0.001246 0.000129 0.001295
Eisosomes 0.000033 0.000016 0.000015 0.000020 0.000014 0.000014 0.000025 0.000025 0.000015 0.000015 0.000016 0.000021
Endoplasmic Reticulum 0.008600 0.006554 0.004046 0.008415 0.003088 0.006306 0.004355 0.006840 0.003522 0.006394 0.004070 0.008256
Endosome 0.001544 0.000500 0.000210 0.001738 0.000639 0.001392 0.000415 0.009867 0.000710 0.001078 0.000225 0.002556
Golgi 0.000073 0.001242 0.000364 0.002473 0.000217 0.000254 0.000034 0.000781 0.000206 0.000602 0.000339 0.002303
Lipid Particles 0.000778 0.000520 0.000068 0.000150 0.000655 0.001169 0.000279 0.000216 0.000665 0.000940 0.000084 0.000156
Mitochondria 0.001427 0.001948 0.001409 0.002160 0.000860 0.003508 0.000416 0.015765 0.000905 0.002958 0.001333 0.003529
Mitotic Spindle 0.000236 0.002283 0.000593 0.005495 0.000714 0.005488 0.000213 0.007942 0.000677 0.004359 0.000563 0.005742
None 0.002297 0.002612 0.001908 0.003286 0.009729 0.002633 0.008170 0.005117 0.009143 0.002626 0.002389 0.003470
Nuclear Periphery 0.000748 0.001309 0.000383 0.002392 0.000815 0.002157 0.000728 0.002552 0.000809 0.001859 0.000409 0.002408
Nuclear Periphery Foci 0.001316 0.000852 0.000057 0.000705 0.000322 0.001468 0.000106 0.000769 0.000400 0.001251 0.000061 0.000712
Nucleolus 0.014584 0.005107 0.003095 0.003873 0.005683 0.005178 0.005779 0.005866 0.006385 0.005153 0.003301 0.004074
Nucleus 0.924013* 0.966060* 0.974995* 0.934844* 0.956703* 0.953980* 0.969860* 0.893094* 0.954127* 0.958235* 0.974600* 0.930643*
Peroxisomes 0.000615 0.001145 0.000086 0.000186 0.000404 0.000577 0.000186 0.000408 0.000421 0.000777 0.000094 0.000209
Vacuole 0.012504 0.000765 0.000697 0.005380 0.000983 0.002831 0.002183 0.012120 0.001891 0.002103 0.000811 0.006059
Vacuole Periphery 0.001612 0.000151 0.000117 0.001322 0.000169 0.000731 0.000409 0.003066 0.000283 0.000527 0.000139 0.001498

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.63 1.81 -1.38 -9.47 -12.19 -3.73 -4.95 -9.54 -9.17 -7.62 -3.14 -5.44 -17.48 -15.83 -14.18
Bud Neck 2.08 1.06 -0.95 -12.94 -8.70 -5.41 -1.22 -6.54 -5.14 -6.18 -3.61 -7.95 -16.37 -13.57 -9.94
Bud Site 1.98 1.86 1.54 -3.86 -2.55 -2.74 -0.30 -2.79 -2.55 -2.77 1.06 0.06 -2.81 -4.39 -3.21
Cell Periphery 0.43 1.12 0.56 0.30 -2.66 1.27 2.92 0.15 -0.98 -2.52 1.48 4.22 1.86 0.20 -3.22
Cytoplasm -0.25 0.25 -2.68 -3.50 -4.30 5.67 4.09 3.56 -1.35 -1.03 6.06 7.02 4.08 -2.83 -4.48
Cytoplasmic Foci -0.37 1.79 0.87 1.44 -2.40 1.92 3.60 1.26 -0.12 -1.38 1.57 3.85 2.53 1.47 -2.72
Eisosomes 3.47 3.99 2.21 -4.27 -8.68 0.36 -5.17 -4.74 -4.83 -2.21 0.74 -0.16 -7.21 -9.03 -8.57
Endoplasmic Reticulum 1.36 2.78 0.31 -3.74 -10.80 -5.71 -2.08 -7.58 -2.55 -3.55 -6.67 -2.24 -12.26 -4.07 -11.47
Endosome 1.90 2.58 0.59 -1.78 -2.84 -1.52 0.64 -1.29 -1.07 -1.37 -0.90 1.80 -1.51 -0.94 -2.97
Golgi -1.78 -0.85 -2.04 -0.25 -1.51 -0.09 1.66 -1.59 -1.69 -2.80 -1.44 -0.30 -1.86 -1.20 -1.58
Lipid Particles 0.56 2.03 1.81 2.59 -2.43 -0.87 2.14 1.15 1.54 -0.98 -0.60 3.54 3.04 2.69 -2.73
Mitochondria -0.73 -0.01 -2.19 -0.67 -1.86 -3.61 1.88 -2.10 -1.37 -2.21 -3.84 -1.00 -4.66 -0.04 -2.77
Mitotic Spindle -1.63 -0.50 -3.93 -2.51 -3.77 -3.40 0.99 -2.64 -1.85 -2.73 -3.59 -0.24 -4.56 -1.99 -4.53
None -0.39 0.49 -1.07 -0.55 -1.95 5.22 0.47 2.88 -1.33 1.08 5.27 5.42 4.77 -0.99 -1.34
Nuclear Periphery -1.42 1.72 -4.10 -2.55 -6.01 -3.23 0.18 -3.55 -0.83 -2.74 -3.52 3.43 -5.12 -1.47 -6.54
Nuclear Periphery Foci 0.47 1.06 0.72 0.63 -3.62 -2.17 2.38 -1.26 -0.14 -1.53 -2.02 2.87 -0.88 1.53 -3.77
Nucleolus 4.59 5.69 4.87 0.87 -1.98 0.70 -0.14 -1.86 -2.17 -1.71 1.77 4.73 1.79 0.26 -2.57
Nucleus -2.53 -3.10 -0.52 6.81 10.92 0.27 -1.68 4.41 4.32 4.89 -1.22 -5.52 5.33 7.16 12.01
Peroxisomes -1.18 3.88 3.29 1.83 -1.63 0.05 1.63 -0.07 -0.15 -2.18 -0.87 2.22 1.74 2.57 -2.51
Vacuole 2.17 2.21 0.99 -6.55 -6.88 -2.80 -0.97 -3.17 -2.55 -2.53 -0.39 2.12 -5.51 -5.49 -7.40
Vacuole Periphery 2.52 2.59 1.07 -2.35 -2.47 -2.78 -0.66 -1.70 -0.95 -1.30 -1.72 1.98 -2.25 -1.35 -2.76
Standard name
Human Ortholog
Description Pre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ecm2

Ecm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available