Standard name
Human Ortholog
Description Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.2 0.16 0.11 0.09 0 0 0 0 0 0 0 0 0 0.06 0.27 0.3 0.21 0.1 0.1 0.13 0.08 0.07 0.07
Bud 0.07 0.08 0 0.06 0.06 0 0.05 0.05 0 0.05 0.06 0.05 0 0 0 0.09 0.12 0.08 0.06 0.06 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.07 0.06 0.05 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.33 0.34 0.26 0.31 0.17 0.11 0.07 0 0 0 0.05 0 0.26 0.26 0.26 0.75 0.63 0.77 0.36 0.45 0.48 0.47 0.46 0.46
Cytoplasm 0.64 0.55 0.7 0.75 0.79 0.84 0.82 0.83 0.78 0.72 0.69 0.68 0.81 0.81 0.83 0.17 0.23 0.17 0.19 0.13 0.12 0.19 0.21 0.22
Endoplasmic Reticulum 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.14 0.11 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.16 0.2 0.23 0.39 0.44 0.49 0.45 0 0 0 0.07 0.11 0.07 0 0 0 0.06 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 53 76 64 24 25 14 12 8 4 6 0 4 40 45 72 153 151 81 43 44 59 32 34 34
Bud 26 31 17 13 17 24 33 37 17 25 23 22 14 14 4 53 60 31 25 28 16 21 22 18
Bud Neck 2 0 6 0 0 2 1 7 0 0 1 3 15 10 15 1 0 0 29 34 32 25 26 26
Bud Site 4 2 3 0 0 2 2 1 0 3 0 3 1 0 0 2 3 0 – – – – – –
Cell Periphery 125 133 105 70 45 58 48 22 13 22 19 13 289 289 303 432 316 296 157 199 218 184 233 222
Cytoplasm 243 211 284 170 211 451 538 613 304 365 289 329 883 883 973 97 115 65 83 57 55 72 108 107
Endoplasmic Reticulum 20 27 11 7 1 0 1 1 1 2 1 1 13 15 25 30 16 10 58 50 26 14 21 19
Endosome 0 0 3 0 1 1 0 1 0 1 0 0 1 1 0 3 3 3 4 3 3 1 5 1
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 2 1 0 1 2 1
Mitochondria 4 7 14 9 14 84 134 168 154 223 204 218 1 1 0 42 55 26 8 3 19 21 29 24
Nucleus 1 1 0 1 0 1 0 3 1 1 1 0 1 1 3 5 5 5 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 1 1 2 0 1 0 1 1 0 1 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 6 2 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 7 4 8 1 4 6 8 5 4 8 8 13 20 20 10 6 8 4 1 1 1 1 2 3
Vac/Vac Membrane 0 1 4 1 2 2 4 5 0 4 0 2 11 12 2 2 3 2 13 11 3 10 11 14
Unique Cell Count 377 387 405 228 268 538 655 738 392 504 418 486 1096 1093 1177 573 504 386 435 439 450 391 507 484
Labelled Cell Count 486 493 520 296 322 654 784 872 500 660 549 608 1291 1293 1407 828 735 527 435 439 450 391 507 484


Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 7.6 5.3 5.3 4.5 3.7 3.3 3.2 2.9 2.9 2.9 2.8 6.7 6.7 6.8 11.6 13.6 15.9 6.5 6.6 6.4
Std Deviation (1e-4) 2.1 2.4 1.6 2.2 1.6 1.7 1.3 1.0 1.0 1.0 1.2 0.8 1.6 1.6 1.6 3.1 4.0 4.4 1.9 2.1 1.7
Intensity Change (Log2) – – – 0.0 -0.23 -0.51 -0.66 -0.7 -0.83 -0.87 -0.84 -0.92 0.35 0.35 0.36 1.14 1.37 1.6 0.31 0.33 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.8 0 0 0 0 0 0 0 0 -8.2 -7.7 -6.0 4.0 5.0 1.9
Bud 0.9 0 0 0 0 0 0 0 0 -3.5 -3.5 -5.9 3.0 4.1 2.3
Bud Neck 0 0 0 0 0 0 0 0 0 -0.2 0 -0.3 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.3 0 0 0 0 0 0 0 0 0.2 0.2 -0.1 15.3 11.1 14.3
Cytoplasm 1.2 2.5 5.0 4.6 5.1 2.4 0.8 -0.3 -0.8 4.3 4.4 5.4 -16.8 -14.3 -15.1
Endoplasmic Reticulum 0.3 0 0 0 0 0 0 0 0 -2.1 -1.8 -0.7 1.9 0.4 -0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 0 0 0 0 0 0 0 0 0 0 2.6 4.2 2.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 -0.2 -0.2 0 -1.2 -0.4 -1.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6757 7.6528 6.6058 7.3038 5.184 6.1283 7.6375 10.0756 8.477 8.8258 7.8913 8.6486 11.1609 12.7502 12.3781 12.9296 12.4284 11.9265
Actin 0.0636 0.0028 0.0027 0.0019 0.0674 0.0133 0.0101 0.0014 0.0086 0.0011 0.0026 0.002 0.0002 0.0044 0.0014 0.0002 0.0005 0.0014
Bud 0.0029 0.0013 0.002 0.0008 0.0053 0.0026 0.0011 0.0055 0.0037 0.0014 0.0007 0.0004 0.0002 0.0024 0.0009 0.0003 0.0004 0.0007
Bud Neck 0.0258 0.002 0.0084 0.007 0.0046 0.0054 0.0092 0.0012 0.0078 0.0279 0.0021 0.0048 0.0004 0.0007 0.0031 0.0011 0.0014 0.0019
Bud Periphery 0.035 0.0092 0.013 0.0148 0.026 0.025 0.012 0.0246 0.0292 0.0169 0.012 0.0107 0.0034 0.0191 0.0078 0.0081 0.0104 0.0188
Bud Site 0.0226 0.0915 0.08 0.0031 0.0209 0.0052 0.0113 0.0481 0.0241 0.0167 0.005 0.0007 0.0091 0.0468 0.0302 0.0005 0.0007 0.0012
Cell Periphery 0.5299 0.6144 0.6648 0.7738 0.5478 0.6045 0.6811 0.7566 0.7278 0.8546 0.8355 0.7523 0.901 0.8031 0.8739 0.9165 0.9254 0.9061
Cytoplasm 0.1518 0.2268 0.1457 0.1432 0.0922 0.2443 0.0809 0.1291 0.1436 0.0597 0.1161 0.1841 0.0391 0.0847 0.0567 0.0153 0.0297 0.0406
Cytoplasmic Foci 0.0092 0.0006 0.0051 0.0058 0.0093 0.0019 0.0154 0.0002 0.0036 0.0001 0.0004 0.0002 0 0.0001 0.0001 0 0.0001 0.0002
Eisosomes 0.005 0.0021 0.0031 0.0006 0.0124 0.0006 0.0034 0.0011 0.0012 0.0009 0.0042 0.0006 0.0033 0.0073 0.0014 0.0004 0.0008 0.0008
Endoplasmic Reticulum 0.0154 0.0161 0.0128 0.0162 0.0347 0.0124 0.022 0.0093 0.0106 0.0059 0.0071 0.0095 0.0317 0.0233 0.0154 0.0485 0.0184 0.0169
Endosome 0.0183 0.0002 0.0066 0.0146 0.0432 0.0044 0.0278 0.0001 0.0029 0 0.0004 0.0001 0 0 0 0 0.0001 0
Golgi 0.0069 0.0004 0.0014 0.003 0.055 0.0012 0.0074 0.0001 0.0019 0.0001 0.0006 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001
Lipid Particles 0.0069 0.0014 0.0018 0.0015 0.006 0.0018 0.0108 0.0007 0.0029 0.0011 0.0022 0.0005 0.0005 0.0005 0.0004 0.0003 0.0005 0.0004
Mitochondria 0.003 0.0001 0.0024 0.0003 0.0457 0.0014 0.0243 0.0001 0.0059 0 0.0005 0.0131 0.0001 0.0002 0.0001 0 0.0002 0.0001
None 0.0451 0.0256 0.0352 0.0004 0.0056 0.0668 0.0517 0.0173 0.0146 0.0003 0.0016 0.0157 0.0009 0.0016 0.0019 0.0001 0.005 0.0045
Nuclear Periphery 0.0088 0.0008 0.0009 0.001 0.0071 0.0011 0.0054 0.0006 0.0007 0.0002 0.0004 0.0003 0.0018 0.0013 0.0009 0.0018 0.0007 0.0005
Nucleolus 0.0016 0.0001 0.0007 0.0001 0.0006 0.0004 0.0011 0.0001 0.0002 0.0002 0.0004 0 0 0.0001 0.0001 0 0.0001 0
Nucleus 0.0231 0.0004 0.0009 0.0004 0.0028 0.0015 0.0019 0.0002 0.0004 0.0004 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001
Peroxisomes 0.002 0 0.0011 0.0001 0.0027 0.0002 0.0061 0 0.0066 0 0.0001 0.0001 0 0 0 0 0 0
Punctate Nuclear 0.0137 0.0001 0.0012 0.0001 0.0015 0.0009 0.0019 0 0.0002 0 0.0001 0 0 0 0 0 0 0
Vacuole 0.0085 0.0042 0.0095 0.0108 0.007 0.0049 0.0137 0.0036 0.0036 0.0121 0.0074 0.0046 0.0081 0.004 0.0055 0.0067 0.0051 0.0054
Vacuole Periphery 0.0009 0.0001 0.0008 0.0002 0.0021 0.0003 0.0015 0 0.0001 0 0.0001 0 0 0 0 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.772 95.9861 126.7111 166.471 83.8369 59.29 105.0943 107.845 122.1282 122.2766
Translational Efficiency 0.7557 0.8351 0.9735 0.63 0.8816 0.8183 0.7689 0.8102 0.7969 0.6864

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cell periphery (20%), cytoplasm (11%), mixed (22%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tat1

Tat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available