Standard name
Human Ortholog
Description Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.08 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.1 0.13 0.14 0 0 0 0 0 0
Cytoplasm 0.75 0.5 0.73 0.82 0.81 0.71 0.7 0.74 0.49 0.55 0.57 0.49 0.78 0.8 0.74 0.56 0.53 0.68 0.26 0.26 0.32 0.36 0.39 0.47
Endoplasmic Reticulum 0 0.41 0.15 0.1 0.05 0.07 0.06 0 0 0 0.05 0 0.17 0.18 0.21 0 0 0 0.39 0.38 0.39 0.25 0.22 0.12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.31 0 0.12 0.13 0.11 0.3 0.44 0.33 0.52 0.49 0.6 0.63 0 0 0 0.1 0.11 0.06 0.13 0.11 0.15 0.15 0.17 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0 0.05 0.06 0 0 0 0 0 0.07 0.15 0.09 0 0.08 0 0.09 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 1 0 1 1 0 0 0 1 5 6 23 9 5 1 4 0 5 11 12
Bud 0 0 0 2 1 1 5 11 1 8 5 14 4 4 3 2 0 0 0 0 0 3 5 7
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3 0 0 0 0 1 1 5 10 9
Bud Site 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 3 1 2 0 4 2 3 0 4 14 10 17 14 14 9 0 1 2 7 14 13
Cytoplasm 12 23 24 86 185 73 127 198 86 147 116 146 175 311 254 83 58 44 10 24 23 73 167 187
Endoplasmic Reticulum 0 19 5 11 12 7 10 2 6 10 10 4 39 70 72 4 3 2 15 35 28 50 95 47
Endosome 0 1 0 1 4 0 0 4 1 3 0 0 0 2 1 5 3 0 2 2 1 2 7 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 2 0 0 1 2
Mitochondria 5 1 4 14 25 31 80 89 90 131 123 187 0 0 1 14 12 4 5 10 11 29 73 53
Nucleus 0 0 0 0 2 0 0 3 5 5 3 4 1 0 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 4 0 0 1 0 0 1 0 1 0 0 0 0 0 1 0
Nucleolus 1 0 0 1 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 1 1 4 1 4 0 0 1 2 0 1 0 0 0 2 4 3
Vac/Vac Membrane 0 2 0 2 13 4 5 10 9 16 5 5 3 5 9 10 16 6 1 7 2 19 31 42
Unique Cell Count 16 46 33 105 227 103 181 269 174 267 205 299 224 389 344 147 110 65 39 94 73 204 429 395
Labelled Cell Count 18 47 33 121 243 119 227 324 209 329 264 370 238 408 369 160 117 72 39 94 73 204 429 395


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 6.3 4.5 4.4 4.7 4.1 3.9 3.8 3.7 3.5 3.4 3.4 6.0 6.0 6.3 6.9 7.0 7.1 6.2 6.2 6.2
Std Deviation (1e-4) 2.4 1.1 1.1 1.1 2.2 1.7 2.6 1.9 1.6 1.0 1.2 1.0 0.9 1.0 1.2 1.0 1.2 1.1 0.9 1.3 1.3
Intensity Change (Log2) – – – -0.03 0.06 -0.13 -0.19 -0.22 -0.25 -0.35 -0.39 -0.4 0.43 0.44 0.51 0.62 0.65 0.66 0.48 0.48 0.47

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.2 -0.2 -0.3 0.1 -2.5 -1.9 -1.7 -2.6 0.7 1.0 0.1 -1.7 -2.0 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.3 0.4 0.8 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3705 1.692 1.695 1.1701 1.4538 1.3257 0.9192 1.692 1.2359 0.6464 0.4293 1.2714 -0.1989 0.043 0.1194 -0.0833 -0.42 -0.022
Actin 0.0189 0.0042 0.0068 0.0059 0.0065 0.0164 0.0255 0.0021 0.0037 0.004 0.0014 0.0122 0.0157 0.0019 0.0122 0.0072 0.0054 0.0006
Bud 0.0004 0.0001 0.0016 0.0002 0.0019 0.0003 0.0002 0.0001 0.0011 0.0001 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0
Bud Neck 0.0028 0.0004 0.001 0.0007 0.0013 0.0061 0.0028 0.0009 0.0009 0.0013 0.0029 0.0153 0.0014 0.0003 0.0016 0.0034 0.0003 0.0008
Bud Periphery 0.0008 0.0002 0.0012 0.0007 0.0024 0.0006 0.0004 0.0002 0.0014 0.0003 0.0005 0.0003 0.0005 0.0002 0.0004 0.0002 0.0001 0
Bud Site 0.0056 0.003 0.0268 0.0004 0.0045 0.0009 0.0026 0.0187 0.0033 0.0003 0.0006 0.0005 0.0016 0.0021 0.0012 0.0003 0.0009 0.0001
Cell Periphery 0.003 0.0027 0.0027 0.0012 0.0009 0.0008 0.0041 0.0037 0.0032 0.0007 0.002 0.0016 0.0017 0.002 0.0057 0.0024 0.0009 0.0005
Cytoplasm 0.2935 0.49 0.3314 0.4356 0.4036 0.3752 0.3139 0.332 0.3629 0.2954 0.384 0.3923 0.2404 0.3837 0.3378 0.3522 0.379 0.4616
Cytoplasmic Foci 0.05 0.0162 0.0299 0.0148 0.0468 0.0242 0.0171 0.0162 0.0284 0.0238 0.0413 0.0113 0.0181 0.026 0.014 0.0108 0.0273 0.0086
Eisosomes 0.0016 0.0003 0.0001 0.0002 0.0003 0.0002 0.0006 0.0003 0.0008 0.0009 0.0001 0.0003 0.0007 0.0002 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.2271 0.2178 0.3372 0.3156 0.1454 0.2691 0.2206 0.2118 0.3019 0.2652 0.3217 0.1539 0.4024 0.3715 0.4227 0.4978 0.3586 0.389
Endosome 0.0407 0.021 0.029 0.0205 0.0849 0.0192 0.053 0.015 0.0421 0.0274 0.0162 0.0064 0.0259 0.0183 0.0164 0.0423 0.0327 0.0144
Golgi 0.0128 0.0023 0.0125 0.0049 0.0372 0.0043 0.0132 0.0024 0.0082 0.0012 0.0017 0.0017 0.0067 0.0016 0.0086 0.0049 0.0073 0.0006
Lipid Particles 0.0392 0.0119 0.0142 0.0074 0.0166 0.0118 0.0181 0.0111 0.0249 0.0059 0.0051 0.0055 0.0403 0.0156 0.0256 0.0143 0.0406 0.0033
Mitochondria 0.0079 0.0004 0.0025 0.0463 0.0125 0.0032 0.0096 0.0006 0.0048 0.0009 0.0005 0.0008 0.0118 0.0003 0.0131 0.0011 0.0015 0.0003
None 0.2487 0.2092 0.1692 0.1204 0.1928 0.2329 0.2721 0.3734 0.181 0.3493 0.2107 0.3818 0.1936 0.153 0.1152 0.0338 0.11 0.1045
Nuclear Periphery 0.0065 0.0094 0.0084 0.0116 0.0085 0.0077 0.0235 0.0032 0.0107 0.0109 0.0033 0.0029 0.0115 0.0109 0.0089 0.0158 0.0077 0.0092
Nucleolus 0.0012 0.0002 0.0004 0.0001 0.0018 0.0003 0.0006 0.0002 0.0005 0.0003 0.0001 0.0002 0.0015 0.0002 0.0002 0.0001 0.0004 0
Nucleus 0.0025 0.0026 0.0032 0.0037 0.0072 0.0087 0.0035 0.0014 0.0046 0.0029 0.0012 0.0019 0.0033 0.0024 0.0015 0.0023 0.0027 0.0019
Peroxisomes 0.0146 0.0004 0.0084 0.0005 0.0076 0.0115 0.0032 0.0002 0.0029 0.0007 0.0009 0.0023 0.01 0.0003 0.0011 0.0001 0.0147 0.0001
Punctate Nuclear 0.0143 0.0012 0.0042 0.0027 0.0039 0.0024 0.0014 0.0012 0.0032 0.0028 0.0013 0.0057 0.0052 0.004 0.0078 0.0007 0.0054 0.0007
Vacuole 0.0069 0.0059 0.0086 0.0054 0.0107 0.0036 0.0103 0.0048 0.0086 0.0051 0.004 0.0028 0.0062 0.0051 0.0047 0.0088 0.0039 0.003
Vacuole Periphery 0.001 0.0005 0.0007 0.001 0.0028 0.0006 0.0037 0.0004 0.0011 0.0005 0.0003 0.0002 0.0012 0.0004 0.0007 0.0011 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.0885 17.9592 8.2009 21.8672 36.0659 32.4351 15.7141 8.4848 19.9305 39.1083
Translational Efficiency 0.583 0.6171 0.6439 0.6279 0.6454 1.1998 0.7969 0.8245 0.5792 0.7226

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2520 1041 108 692 1719 1437 1819 1176 4239 2478 1927 1868

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 732.41 776.24 942.41 913.80 748.62 736.96 824.08 890.37 738.98 753.46 830.71 899.05
Standard Deviation 100.48 107.21 105.64 113.38 97.45 100.65 107.94 126.60 99.58 105.26 111.20 122.39
Intensity Change Log 2 — 0.083851 0.363703 0.319227 — -0.022647 0.138551 0.250171 — 0.031002 0.254285 0.284734

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000272 0.000455 0.000798 0.002412 0.000365 0.001149 0.001134 0.001745 0.000309 0.000858 0.001115 0.001992
Bud Neck 0.014365 0.008880 0.002685 0.009769 0.010513 0.023802 0.020616 0.012341 0.012803 0.017533 0.019611 0.011388
Bud Site 0.006385 0.007513 0.012383 0.039758 0.010638 0.020035 0.033306 0.031215 0.008110 0.014775 0.032133 0.034380
Cell Periphery 0.000554 0.000486 0.000600 0.001076 0.000706 0.000812 0.000632 0.001020 0.000615 0.000675 0.000630 0.001041
Cytoplasm 0.425504 0.435691 0.378779 0.347705 0.423618 0.338681 0.474218 0.436949 0.424739 0.379435 0.468869 0.403889
Cytoplasmic Foci 0.106238 0.205199 0.062469 0.059214 0.168376 0.246264 0.049083 0.077721 0.131436 0.229013 0.049833 0.070865
Eisosomes 0.000607 0.000420 0.000162 0.000417 0.000774 0.000986 0.000231 0.000501 0.000675 0.000748 0.000227 0.000470
Endoplasmic Reticulum 0.101989 0.044188 0.023487 0.036519 0.107495 0.051071 0.029985 0.035121 0.104222 0.048180 0.029621 0.035639
Endosome 0.012954 0.020258 0.013562 0.008251 0.014664 0.033360 0.006267 0.006856 0.013648 0.027856 0.006676 0.007373
Golgi 0.006619 0.005455 0.000303 0.001795 0.008824 0.015768 0.000878 0.002095 0.007513 0.011436 0.000845 0.001984
Lipid Particles 0.049364 0.041733 0.009874 0.012755 0.061353 0.074701 0.009977 0.015998 0.054226 0.060851 0.009972 0.014797
Mitochondria 0.005461 0.002888 0.000349 0.002058 0.006065 0.013846 0.002020 0.002916 0.005706 0.009242 0.001926 0.002598
Mitotic Spindle 0.000297 0.000567 0.004940 0.010328 0.001557 0.003258 0.009697 0.006049 0.000808 0.002127 0.009430 0.007634
None 0.008565 0.002210 0.001750 0.001162 0.004888 0.002468 0.002410 0.004719 0.007074 0.002359 0.002373 0.003401
Nuclear Periphery 0.004984 0.002167 0.003461 0.002124 0.002890 0.001628 0.004627 0.002334 0.004135 0.001855 0.004562 0.002256
Nuclear Periphery Foci 0.003937 0.006858 0.021832 0.021216 0.005403 0.002648 0.010234 0.015267 0.004532 0.004416 0.010884 0.017471
Nucleolus 0.001918 0.001036 0.000643 0.000272 0.000920 0.001089 0.000427 0.000431 0.001513 0.001067 0.000439 0.000372
Nucleus 0.170189 0.106040 0.279104 0.165484 0.102446 0.078009 0.215436 0.175394 0.142718 0.089785 0.219004 0.171722
Peroxisomes 0.003021 0.004624 0.001779 0.001173 0.003499 0.011148 0.001816 0.002469 0.003215 0.008407 0.001814 0.001989
Vacuole 0.075608 0.101807 0.180767 0.276016 0.063456 0.076059 0.126007 0.168344 0.070680 0.086876 0.129076 0.208231
Vacuole Periphery 0.001169 0.001526 0.000273 0.000497 0.001549 0.003218 0.001002 0.000516 0.001323 0.002507 0.000961 0.000509

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.80 -3.91 -4.16 -3.70 -2.64 -4.69 -3.89 -5.79 -1.90 -1.69 -5.44 -4.34 -7.08 -4.08 -2.31
Bud Neck 4.45 10.78 2.93 -0.80 -5.82 -6.78 -4.57 -0.67 5.77 3.71 -3.70 -3.23 1.60 4.73 4.10
Bud Site -0.81 -0.92 -6.24 -5.90 -3.56 -3.50 -6.52 -5.24 -2.14 0.66 -4.26 -8.13 -8.60 -5.46 -0.18
Cell Periphery 1.57 -0.69 -4.50 -5.09 -2.61 -0.90 1.73 -2.07 -0.61 -3.32 -0.84 0.39 -4.71 -2.69 -4.31
Cytoplasm -1.07 3.03 10.68 9.99 1.37 10.25 -4.14 1.67 -6.92 5.05 7.56 -3.90 7.41 0.89 9.23
Cytoplasmic Foci -16.74 6.50 14.33 25.96 0.77 -12.43 27.67 19.26 29.36 -6.87 -22.62 27.56 20.82 38.23 -5.54
Eisosomes 3.78 9.80 4.79 1.39 -4.18 -2.29 8.09 4.35 7.35 -5.51 -1.34 11.74 5.93 7.04 -6.13
Endoplasmic Reticulum 10.79 11.17 15.12 3.16 -0.84 9.24 14.72 13.65 4.64 -0.79 13.96 20.60 20.25 5.55 -0.87
Endosome -5.77 -0.54 7.20 10.20 3.56 -10.44 11.81 11.65 15.58 -0.55 -12.14 12.63 13.50 18.50 -0.20
Golgi 1.61 15.48 9.10 5.45 -1.95 -5.87 14.50 9.67 12.00 -1.57 -5.19 18.67 12.36 12.93 -1.78
Lipid Particles 2.81 18.27 20.06 12.56 -0.45 -3.58 22.64 19.52 18.92 -4.18 -2.67 30.26 27.02 22.21 -3.70
Mitochondria 4.56 19.56 5.77 1.74 -1.63 -5.00 5.27 3.29 7.12 -0.56 -3.94 8.72 5.44 7.09 -0.40
Mitotic Spindle -1.47 -1.55 -4.15 -4.01 -1.53 -1.46 -4.35 -3.04 -1.54 1.67 -2.10 -5.21 -5.49 -3.89 0.97
None 6.56 6.83 7.88 3.45 1.09 2.98 3.43 0.76 -1.14 -1.41 6.88 6.52 4.51 -0.60 -0.85
Nuclear Periphery 5.17 1.95 5.04 -0.56 2.64 3.09 -2.70 1.01 -2.19 3.64 6.31 -0.69 4.50 -2.13 4.10
Nuclear Periphery Foci -4.75 -6.01 -9.43 -7.19 1.24 5.78 -5.42 -7.87 -10.12 -3.40 0.28 -7.79 -12.36 -12.13 -4.45
Nucleolus 4.07 5.89 9.20 6.32 3.45 -1.26 6.08 5.24 4.48 -0.25 3.09 9.07 9.74 7.02 1.24
Nucleus 13.79 -4.73 1.62 -6.86 5.06 6.54 -20.54 -11.99 -16.10 5.74 17.41 -14.54 -5.30 -16.60 8.00
Peroxisomes -3.33 2.35 8.18 8.39 1.03 -11.55 4.56 2.55 11.38 -0.81 -11.78 4.25 4.43 13.12 -0.01
Vacuole -6.55 -6.81 -24.83 -21.50 -6.30 -3.65 -18.20 -21.72 -19.47 -8.13 -6.27 -18.64 -31.47 -27.85 -15.06
Vacuole Periphery -1.48 13.00 7.24 4.73 -0.89 -4.23 1.94 4.93 7.60 1.40 -4.97 1.68 7.59 8.82 1.50
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant
Localization
Cell Percentages ER (71%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Alg14

Alg14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
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