Standard name
Human Ortholog
Description Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.08 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.1 0.13 0.14 0 0 0 0 0 0
Cytoplasm 0.75 0.5 0.73 0.82 0.81 0.71 0.7 0.74 0.49 0.55 0.57 0.49 0.78 0.8 0.74 0.56 0.53 0.68 0.26 0.26 0.32 0.36 0.39 0.47
Endoplasmic Reticulum 0 0.41 0.15 0.1 0.05 0.07 0.06 0 0 0 0.05 0 0.17 0.18 0.21 0 0 0 0.39 0.38 0.39 0.25 0.22 0.12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.31 0 0.12 0.13 0.11 0.3 0.44 0.33 0.52 0.49 0.6 0.63 0 0 0 0.1 0.11 0.06 0.13 0.11 0.15 0.15 0.17 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0 0.05 0.06 0 0 0 0 0 0.07 0.15 0.09 0 0.08 0 0.09 0.07 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 1 0 1 1 0 0 0 1 5 6 23 9 5 1 4 0 5 11 12
Bud 0 0 0 2 1 1 5 11 1 8 5 14 4 4 3 2 0 0 0 0 0 3 5 7
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3 0 0 0 0 1 1 5 10 9
Bud Site 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0
Cell Periphery 0 0 0 3 1 2 0 4 2 3 0 4 14 10 17 14 14 9 0 1 2 7 14 13
Cytoplasm 12 23 24 86 185 73 127 198 86 147 116 146 175 311 254 83 58 44 10 24 23 73 167 187
Endoplasmic Reticulum 0 19 5 11 12 7 10 2 6 10 10 4 39 70 72 4 3 2 15 35 28 50 95 47
Endosome 0 1 0 1 4 0 0 4 1 3 0 0 0 2 1 5 3 0 2 2 1 2 7 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 2 0 0 1 2
Mitochondria 5 1 4 14 25 31 80 89 90 131 123 187 0 0 1 14 12 4 5 10 11 29 73 53
Nucleus 0 0 0 0 2 0 0 3 5 5 3 4 1 0 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 4 0 0 1 0 0 1 0 1 0 0 0 0 0 1 0
Nucleolus 1 0 0 1 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 1 1 4 1 4 0 0 1 2 0 1 0 0 0 2 4 3
Vac/Vac Membrane 0 2 0 2 13 4 5 10 9 16 5 5 3 5 9 10 16 6 1 7 2 19 31 42
Unique Cell Count 16 46 33 105 227 103 181 269 174 267 205 299 224 389 344 147 110 65 39 94 73 204 429 395
Labelled Cell Count 18 47 33 121 243 119 227 324 209 329 264 370 238 408 369 160 117 72 39 94 73 204 429 395


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 6.3 4.5 4.4 4.7 4.1 3.9 3.8 3.7 3.5 3.4 3.4 6.0 6.0 6.3 6.9 7.0 7.1 6.2 6.2 6.2
Std Deviation (1e-4) 2.4 1.1 1.1 1.1 2.2 1.7 2.6 1.9 1.6 1.0 1.2 1.0 0.9 1.0 1.2 1.0 1.2 1.1 0.9 1.3 1.3
Intensity Change (Log2) -0.03 0.06 -0.13 -0.19 -0.22 -0.25 -0.35 -0.39 -0.4 0.43 0.44 0.51 0.62 0.65 0.66 0.48 0.48 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.2 -0.2 -0.3 0.1 -2.5 -1.9 -1.7 -2.6 0.7 1.0 0.1 -1.7 -2.0 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.3 0.4 0.8 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3705 1.692 1.695 1.1701 1.4538 1.3257 0.9192 1.692 1.2359 0.6464 0.4293 1.2714 -0.1989 0.043 0.1194 -0.0833 -0.42 -0.022
Actin 0.0189 0.0042 0.0068 0.0059 0.0065 0.0164 0.0255 0.0021 0.0037 0.004 0.0014 0.0122 0.0157 0.0019 0.0122 0.0072 0.0054 0.0006
Bud 0.0004 0.0001 0.0016 0.0002 0.0019 0.0003 0.0002 0.0001 0.0011 0.0001 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0
Bud Neck 0.0028 0.0004 0.001 0.0007 0.0013 0.0061 0.0028 0.0009 0.0009 0.0013 0.0029 0.0153 0.0014 0.0003 0.0016 0.0034 0.0003 0.0008
Bud Periphery 0.0008 0.0002 0.0012 0.0007 0.0024 0.0006 0.0004 0.0002 0.0014 0.0003 0.0005 0.0003 0.0005 0.0002 0.0004 0.0002 0.0001 0
Bud Site 0.0056 0.003 0.0268 0.0004 0.0045 0.0009 0.0026 0.0187 0.0033 0.0003 0.0006 0.0005 0.0016 0.0021 0.0012 0.0003 0.0009 0.0001
Cell Periphery 0.003 0.0027 0.0027 0.0012 0.0009 0.0008 0.0041 0.0037 0.0032 0.0007 0.002 0.0016 0.0017 0.002 0.0057 0.0024 0.0009 0.0005
Cytoplasm 0.2935 0.49 0.3314 0.4356 0.4036 0.3752 0.3139 0.332 0.3629 0.2954 0.384 0.3923 0.2404 0.3837 0.3378 0.3522 0.379 0.4616
Cytoplasmic Foci 0.05 0.0162 0.0299 0.0148 0.0468 0.0242 0.0171 0.0162 0.0284 0.0238 0.0413 0.0113 0.0181 0.026 0.014 0.0108 0.0273 0.0086
Eisosomes 0.0016 0.0003 0.0001 0.0002 0.0003 0.0002 0.0006 0.0003 0.0008 0.0009 0.0001 0.0003 0.0007 0.0002 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.2271 0.2178 0.3372 0.3156 0.1454 0.2691 0.2206 0.2118 0.3019 0.2652 0.3217 0.1539 0.4024 0.3715 0.4227 0.4978 0.3586 0.389
Endosome 0.0407 0.021 0.029 0.0205 0.0849 0.0192 0.053 0.015 0.0421 0.0274 0.0162 0.0064 0.0259 0.0183 0.0164 0.0423 0.0327 0.0144
Golgi 0.0128 0.0023 0.0125 0.0049 0.0372 0.0043 0.0132 0.0024 0.0082 0.0012 0.0017 0.0017 0.0067 0.0016 0.0086 0.0049 0.0073 0.0006
Lipid Particles 0.0392 0.0119 0.0142 0.0074 0.0166 0.0118 0.0181 0.0111 0.0249 0.0059 0.0051 0.0055 0.0403 0.0156 0.0256 0.0143 0.0406 0.0033
Mitochondria 0.0079 0.0004 0.0025 0.0463 0.0125 0.0032 0.0096 0.0006 0.0048 0.0009 0.0005 0.0008 0.0118 0.0003 0.0131 0.0011 0.0015 0.0003
None 0.2487 0.2092 0.1692 0.1204 0.1928 0.2329 0.2721 0.3734 0.181 0.3493 0.2107 0.3818 0.1936 0.153 0.1152 0.0338 0.11 0.1045
Nuclear Periphery 0.0065 0.0094 0.0084 0.0116 0.0085 0.0077 0.0235 0.0032 0.0107 0.0109 0.0033 0.0029 0.0115 0.0109 0.0089 0.0158 0.0077 0.0092
Nucleolus 0.0012 0.0002 0.0004 0.0001 0.0018 0.0003 0.0006 0.0002 0.0005 0.0003 0.0001 0.0002 0.0015 0.0002 0.0002 0.0001 0.0004 0
Nucleus 0.0025 0.0026 0.0032 0.0037 0.0072 0.0087 0.0035 0.0014 0.0046 0.0029 0.0012 0.0019 0.0033 0.0024 0.0015 0.0023 0.0027 0.0019
Peroxisomes 0.0146 0.0004 0.0084 0.0005 0.0076 0.0115 0.0032 0.0002 0.0029 0.0007 0.0009 0.0023 0.01 0.0003 0.0011 0.0001 0.0147 0.0001
Punctate Nuclear 0.0143 0.0012 0.0042 0.0027 0.0039 0.0024 0.0014 0.0012 0.0032 0.0028 0.0013 0.0057 0.0052 0.004 0.0078 0.0007 0.0054 0.0007
Vacuole 0.0069 0.0059 0.0086 0.0054 0.0107 0.0036 0.0103 0.0048 0.0086 0.0051 0.004 0.0028 0.0062 0.0051 0.0047 0.0088 0.0039 0.003
Vacuole Periphery 0.001 0.0005 0.0007 0.001 0.0028 0.0006 0.0037 0.0004 0.0011 0.0005 0.0003 0.0002 0.0012 0.0004 0.0007 0.0011 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.0885 17.9592 8.2009 21.8672 36.0659 32.4351 15.7141 8.4848 19.9305 39.1083
Translational Efficiency 0.583 0.6171 0.6439 0.6279 0.6454 1.1998 0.7969 0.8245 0.5792 0.7226

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant
Localization
Cell Percentages ER (71%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Alg14

Alg14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available