Standard name | |
---|---|
Human Ortholog | |
Description | eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation; eIF3 is also involved in programmed stop codon readthrough |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 2 | 1 | 7 | 9 | 24 | 12 | 38 | 26 | 34 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 2 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 3 | 3 | 14 | 22 | 26 | 33 | 14 | 35 | 31 | 40 | 0 | 0 | 0 | 10 | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 76 | 143 | 287 | 355 | 347 | 374 | 359 | 355 | 241 | 276 | 236 | 245 | 97 | 110 | 116 | 372 | 221 | 314 | 82 | 142 | 296 | 38 | 119 | 118 |
Endoplasmic Reticulum | 1 | 2 | 11 | 6 | 29 | 37 | 47 | 75 | 46 | 84 | 57 | 58 | 2 | 4 | 0 | 19 | 13 | 13 | 0 | 0 | 11 | 4 | 8 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 13 | 4 | 2 | 9 | 9 | 0 | 0 | 0 | 3 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 6 | 8 | 16 | 33 | 49 | 55 | 54 | 2 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 1 | 0 | 4 | 11 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 5 | 2 | 3 | 0 | 2 | 4 | 0 | 1 | 1 |
Unique Cell Count | 76 | 145 | 298 | 361 | 378 | 421 | 428 | 477 | 316 | 405 | 346 | 380 | 101 | 114 | 118 | 385 | 233 | 323 | 84 | 150 | 321 | 45 | 136 | 143 |
Labelled Cell Count | 78 | 146 | 302 | 367 | 394 | 448 | 455 | 532 | 353 | 488 | 420 | 445 | 101 | 114 | 119 | 414 | 249 | 340 | 84 | 150 | 321 | 45 | 136 | 143 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 88.5 | 60.9 | 42.6 | 42.1 | 37.6 | 30.8 | 26.6 | 26.4 | 23.6 | 22.1 | 21.5 | 21.2 | 64.1 | 57.2 | 48.2 | 56.7 | 64.6 | 67.3 | 75.7 | 72.5 | 70.7 |
Std Deviation (1e-4) | 14.2 | 13.3 | 8.2 | 7.5 | 7.6 | 5.4 | 5.6 | 5.5 | 5.5 | 4.9 | 4.9 | 4.6 | 14.1 | 14.3 | 12.2 | 16.5 | 17.0 | 17.9 | 13.7 | 19.6 | 25.3 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.18 | -0.47 | -0.68 | -0.69 | -0.85 | -0.95 | -0.99 | -1.0 | 0.59 | 0.42 | 0.18 | 0.41 | 0.6 | 0.66 | 0.83 | 0.77 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.9 | 3.4 | 5.4 | 4.8 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.2 | 3.0 | 3.4 | 3.8 | 2.6 | 4.4 | 4.5 | 5.0 | 0 | 0 | 0 | 1.5 | 2.2 | 1.4 |
Cytoplasm | 1.6 | -2.4 | -3.6 | -5.3 | -7.9 | -7.2 | -9.2 | -9.1 | -10.0 | -0.1 | 0.1 | 1.1 | 0.2 | -0.8 | 0.6 |
Endoplasmic Reticulum | -1.6 | 2.2 | 2.7 | 3.6 | 5.2 | 4.6 | 6.5 | 5.3 | 4.9 | 0 | 0 | 0 | 0.8 | 1.0 | 0.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.2 | 5.7 | 6.2 | 7.2 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 65.4039 | 90.5898 | 79.575 | 78.6766 | 74.5945 | 84.7701 | 63.0338 | 87.7577 | 78.85 | 70.9957 | 47.6875 | 73.7089 | 67.6604 | 85.1312 | 74.0297 | 72.6986 | 75.0767 | 72.2624 |
Actin | 0.0088 | 0.0003 | 0.0036 | 0.0001 | 0.0033 | 0.0005 | 0.0102 | 0.0001 | 0.0005 | 0.0012 | 0.0052 | 0.0008 | 0.0032 | 0.0013 | 0.0015 | 0.0003 | 0.0007 | 0.003 |
Bud | 0.0017 | 0.0008 | 0.0017 | 0.0011 | 0.0005 | 0.0005 | 0.0031 | 0.0003 | 0.0009 | 0.0014 | 0.0129 | 0.0005 | 0.0023 | 0.0013 | 0.0023 | 0.0022 | 0.0047 | 0.0017 |
Bud Neck | 0.0009 | 0.0009 | 0.0019 | 0.0013 | 0.002 | 0.003 | 0.0006 | 0.0002 | 0.0004 | 0.0006 | 0.0011 | 0.0011 | 0.0007 | 0.0005 | 0.0007 | 0.0006 | 0.0007 | 0.001 |
Bud Periphery | 0.0028 | 0.0008 | 0.0027 | 0.0012 | 0.0004 | 0.0005 | 0.0032 | 0.0001 | 0.0013 | 0.0005 | 0.013 | 0.0005 | 0.0026 | 0.001 | 0.0012 | 0.0009 | 0.004 | 0.0006 |
Bud Site | 0.0014 | 0.0004 | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.003 | 0.0002 | 0.0004 | 0.0003 | 0.0251 | 0 | 0.001 | 0.0008 | 0.0006 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.024 | 0.0151 | 0.0181 | 0.0183 | 0.0044 | 0.0015 | 0.0052 | 0.0002 | 0.0032 | 0.0004 | 0.0022 | 0.0002 | 0.0063 | 0.0044 | 0.0025 | 0.0034 | 0.0009 | 0.0003 |
Cytoplasm | 0.4771 | 0.7879 | 0.6762 | 0.4154 | 0.5778 | 0.7065 | 0.8283 | 0.9754 | 0.9468 | 0.8676 | 0.5735 | 0.8777 | 0.6851 | 0.9471 | 0.9122 | 0.5974 | 0.8008 | 0.8806 |
Cytoplasmic Foci | 0.0138 | 0.0009 | 0.0055 | 0.0009 | 0.0138 | 0.0022 | 0.0118 | 0.0055 | 0.0036 | 0.0054 | 0.0387 | 0.0064 | 0.016 | 0.0015 | 0.0043 | 0.0032 | 0.0049 | 0.0085 |
Eisosomes | 0.0015 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1033 | 0.0485 | 0.0464 | 0.0936 | 0.0483 | 0.1236 | 0.0369 | 0.0057 | 0.0045 | 0.0082 | 0.0204 | 0.0636 | 0.0943 | 0.0227 | 0.0154 | 0.0337 | 0.0054 | 0.0089 |
Endosome | 0.0157 | 0.0031 | 0.0128 | 0.0105 | 0.017 | 0.0165 | 0.0193 | 0.002 | 0.0073 | 0.0187 | 0.0687 | 0.0219 | 0.0125 | 0.0019 | 0.0135 | 0.0332 | 0.0285 | 0.043 |
Golgi | 0.0041 | 0.0003 | 0.0017 | 0.0002 | 0.0074 | 0.0014 | 0.0047 | 0.0002 | 0.0006 | 0.0012 | 0.0036 | 0.0031 | 0.0034 | 0.001 | 0.0029 | 0.002 | 0.0023 | 0.0065 |
Lipid Particles | 0.0095 | 0.0006 | 0.0029 | 0.0004 | 0.0052 | 0.0001 | 0.0023 | 0.0004 | 0.0006 | 0.0015 | 0.0088 | 0.0001 | 0.0028 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0001 |
Mitochondria | 0.0217 | 0.0012 | 0.0151 | 0.0005 | 0.0585 | 0.0021 | 0.008 | 0.0002 | 0.0028 | 0.0008 | 0.0619 | 0.0067 | 0.0241 | 0.0002 | 0.0038 | 0.0019 | 0.0107 | 0.0021 |
None | 0.0043 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0003 | 0.0019 | 0.0005 | 0.0009 | 0.0009 | 0.0101 | 0.0006 | 0.0085 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0004 |
Nuclear Periphery | 0.0392 | 0.0095 | 0.0165 | 0.0348 | 0.0069 | 0.0149 | 0.0096 | 0.0007 | 0.0015 | 0.0124 | 0.0129 | 0.0047 | 0.0389 | 0.0018 | 0.0026 | 0.0329 | 0.0092 | 0.0036 |
Nucleolus | 0.0067 | 0.0004 | 0.0012 | 0.0001 | 0.0002 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0037 | 0 | 0.0078 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 |
Nucleus | 0.0116 | 0.003 | 0.0041 | 0.0055 | 0.0026 | 0.0031 | 0.0047 | 0.0006 | 0.001 | 0.004 | 0.0068 | 0.0011 | 0.0153 | 0.0012 | 0.0014 | 0.0038 | 0.002 | 0.0013 |
Peroxisomes | 0.0039 | 0 | 0.0005 | 0 | 0.0144 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.007 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0.0011 | 0.0001 | 0.0031 | 0.002 | 0 | 0.0044 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Vacuole | 0.1808 | 0.1071 | 0.1493 | 0.3068 | 0.1688 | 0.079 | 0.0346 | 0.0055 | 0.0195 | 0.0579 | 0.0887 | 0.0072 | 0.0452 | 0.0116 | 0.026 | 0.1976 | 0.0549 | 0.0233 |
Vacuole Periphery | 0.0601 | 0.0189 | 0.0368 | 0.109 | 0.0677 | 0.044 | 0.0106 | 0.0009 | 0.004 | 0.0137 | 0.039 | 0.0038 | 0.0249 | 0.0013 | 0.0085 | 0.0855 | 0.069 | 0.0148 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 186.3782 | 202.0453 | 186.0805 | 156.932 | 170.4875 | 164.181 | 164.1537 | 137.1829 | 132.2328 | 165.8152 |
Translational Efficiency | 1.3911 | 1.3098 | 1.0978 | 1.3987 | 1.3485 | 1.0826 | 1.1107 | 1.0359 | 1.1173 | 1.2277 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1330 | 1009 | 1734 | 1095 | 1305 | 1394 | 40 | 863 | 2635 | 2403 | 1774 | 1958 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3624.10 | 4377.91 | 5790.28 | 5771.60 | 3680.84 | 4277.17 | 6396.77 | 5597.93 | 3652.20 | 4319.47 | 5803.96 | 5695.05 |
Standard Deviation | 687.06 | 1035.20 | 845.06 | 1451.51 | 791.44 | 898.78 | 980.91 | 1512.06 | 741.14 | 959.72 | 853.13 | 1481.01 |
Intensity Change Log 2 | — | 0.272620 | 0.676010 | 0.671349 | — | 0.216622 | 0.797309 | 0.604858 | — | 0.244675 | 0.738405 | 0.638228 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000062 | 0.000111 | 0.000072 | 0.000788 | 0.000051 | 0.000087 | 0.000390 | 0.000210 | 0.000056 | 0.000097 | 0.000079 | 0.000533 |
Bud Neck | 0.000186 | 0.000574 | 0.001568 | 0.002079 | 0.000288 | 0.000424 | 0.009816 | 0.002001 | 0.000236 | 0.000487 | 0.001754 | 0.002045 |
Bud Site | 0.002213 | 0.004115 | 0.001832 | 0.010524 | 0.001921 | 0.003125 | 0.006570 | 0.008746 | 0.002069 | 0.003541 | 0.001939 | 0.009740 |
Cell Periphery | 0.007104 | 0.012964 | 0.007706 | 0.013677 | 0.011288 | 0.006257 | 0.031741 | 0.028195 | 0.009176 | 0.009073 | 0.008248 | 0.020076 |
Cytoplasm | 0.786583* | 0.727011* | 0.579452* | 0.495673* | 0.655024* | 0.809231* | 0.228780* | 0.476133* | 0.721428* | 0.774708* | 0.571545* | 0.487060* |
Cytoplasmic Foci | 0.007252 | 0.010958 | 0.009459 | 0.007189 | 0.009323 | 0.010057 | 0.031700 | 0.006988 | 0.008278 | 0.010435 | 0.009960 | 0.007101 |
Eisosomes | 0.000001 | 0.000002 | 0.000002 | 0.000006 | 0.000001 | 0.000001 | 0.000013 | 0.000005 | 0.000001 | 0.000001 | 0.000002 | 0.000006 |
Endoplasmic Reticulum | 0.000604 | 0.001202 | 0.001123 | 0.000335 | 0.001946 | 0.000749 | 0.000228 | 0.000362 | 0.001269 | 0.000939 | 0.001103 | 0.000347 |
Endosome | 0.000459 | 0.001126 | 0.000817 | 0.003550 | 0.000918 | 0.000799 | 0.001605 | 0.001871 | 0.000686 | 0.000936 | 0.000835 | 0.002810 |
Golgi | 0.063692 | 0.084732 | 0.071805 | 0.026838 | 0.097943 | 0.061105 | 0.081060 | 0.030192 | 0.080655 | 0.071026 | 0.072014 | 0.028316 |
Lipid Particles | 0.003810 | 0.014593 | 0.007329 | 0.013906 | 0.010563 | 0.007909 | 0.026987 | 0.022738 | 0.007154 | 0.010716 | 0.007773 | 0.017799 |
Mitochondria | 0.000195 | 0.000123 | 0.000211 | 0.002596 | 0.000130 | 0.000155 | 0.000193 | 0.000994 | 0.000163 | 0.000141 | 0.000211 | 0.001890 |
Mitotic Spindle | 0.000564 | 0.001452 | 0.003362 | 0.004854 | 0.000816 | 0.001060 | 0.006012 | 0.000955 | 0.000689 | 0.001224 | 0.003422 | 0.003136 |
None | 0.002094 | 0.002087 | 0.001741 | 0.002572 | 0.002064 | 0.001995 | 0.001104 | 0.001941 | 0.002079 | 0.002034 | 0.001727 | 0.002294 |
Nuclear Periphery | 0.000228 | 0.000648 | 0.002488 | 0.000537 | 0.000740 | 0.000347 | 0.005131 | 0.000477 | 0.000481 | 0.000474 | 0.002548 | 0.000510 |
Nuclear Periphery Foci | 0.007625 | 0.019389 | 0.025130 | 0.006146 | 0.022335 | 0.011605 | 0.024461 | 0.007597 | 0.014910 | 0.014874 | 0.025115 | 0.006785 |
Nucleolus | 0.000319 | 0.000082 | 0.000100 | 0.000397 | 0.000080 | 0.000054 | 0.000215 | 0.000381 | 0.000201 | 0.000066 | 0.000103 | 0.000390 |
Nucleus | 0.004381 | 0.006500 | 0.008714 | 0.011365 | 0.004561 | 0.005397 | 0.043130 | 0.010805 | 0.004470 | 0.005860 | 0.009490 | 0.011118 |
Peroxisomes | 0.000020 | 0.000022 | 0.000008 | 0.000361 | 0.000013 | 0.000015 | 0.000023 | 0.000111 | 0.000016 | 0.000018 | 0.000008 | 0.000251 |
Vacuole | 0.027564 | 0.058669 | 0.127084 | 0.332143* | 0.065504 | 0.038427 | 0.389608* | 0.346585* | 0.046354 | 0.046927 | 0.133003 | 0.338508* |
Vacuole Periphery | 0.085045 | 0.053639 | 0.149998* | 0.064465 | 0.114491* | 0.041200 | 0.111232* | 0.052712 | 0.099629 | 0.046423 | 0.149124* | 0.059285 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.43 | -1.20 | -1.61 | -1.44 | -1.56 | -2.72 | -2.38 | -5.91 | -4.40 | 1.46 | -3.37 | -2.67 | -1.93 | -1.72 | -1.78 |
Bud Neck | -5.19 | -9.10 | -7.08 | -5.79 | -1.72 | -2.79 | -3.28 | -6.41 | -5.94 | 2.71 | -5.86 | -9.35 | -9.51 | -8.33 | -1.10 |
Bud Site | -3.23 | 0.61 | -6.62 | -4.78 | -6.83 | -2.32 | -1.81 | -7.04 | -5.95 | -0.22 | -3.96 | -0.11 | -9.12 | -7.20 | -9.20 |
Cell Periphery | -5.23 | -1.11 | -4.45 | 0.57 | -4.03 | 5.83 | -3.10 | -6.96 | -9.86 | 0.99 | 0.04 | 1.10 | -7.94 | -7.92 | -8.67 |
Cytoplasm | 4.81 | 18.44 | 22.37 | 15.96 | 5.56 | -12.48 | 9.08 | 11.45 | 23.42 | -5.26 | -5.77 | 14.89 | 23.15 | 28.81 | 6.37 |
Cytoplasmic Foci | -3.99 | -3.70 | -0.60 | 2.93 | 2.49 | -0.90 | -2.77 | 2.06 | 3.10 | 2.96 | -3.42 | -3.33 | 0.92 | 4.05 | 3.91 |
Eisosomes | -3.70 | -3.61 | -4.43 | -3.62 | -3.46 | 0.29 | -3.88 | -5.53 | -5.61 | 2.83 | -2.75 | -4.23 | -6.33 | -5.79 | -4.41 |
Endoplasmic Reticulum | -2.80 | -2.79 | 4.18 | 4.37 | 4.73 | 3.67 | 5.40 | 5.24 | 3.48 | -1.06 | 1.74 | 0.84 | 6.05 | 5.64 | 4.66 |
Endosome | -6.05 | -6.73 | -3.38 | -2.48 | -2.85 | 1.14 | -1.94 | -2.39 | -2.66 | -0.23 | -3.94 | -3.33 | -3.94 | -3.35 | -3.50 |
Golgi | -4.03 | -2.10 | 10.84 | 14.06 | 18.89* | 7.70 | 1.85 | 17.27 | 10.14 | 3.68 | 2.77 | 2.82 | 19.97 | 17.13 | 20.74* |
Lipid Particles | -9.08 | -7.07 | -8.77 | 0.87 | -4.93 | 2.32 | -2.97 | -6.86 | -8.81 | 1.34 | -4.74 | -2.01 | -10.30 | -5.91 | -9.01 |
Mitochondria | 1.03 | -0.47 | -2.35 | -2.44 | -2.32 | -0.72 | -1.54 | -1.70 | -1.65 | -1.49 | 0.55 | -1.61 | -2.84 | -2.89 | -2.72 |
Mitotic Spindle | -7.30 | -8.21 | -2.74 | -2.14 | -0.58 | -2.39 | -2.13 | -0.83 | 0.01 | 2.02 | -6.75 | -8.15 | -2.82 | -2.17 | 0.80 |
None | -0.02 | 1.94 | -0.42 | -0.40 | -0.89 | 0.30 | 2.52 | 0.45 | 0.31 | -1.07 | 0.21 | 2.91 | -0.16 | -0.24 | -0.88 |
Nuclear Periphery | -7.48 | -14.38 | -3.82 | 0.89 | 11.20 | 5.26 | -4.24 | 2.49 | -2.53 | 4.43 | 0.01 | -13.25 | -1.00 | -1.04 | 12.51 |
Nuclear Periphery Foci | -8.58 | -16.23 | 1.81 | 9.46 | 17.28 | 6.78 | -0.33 | 9.76 | 3.75 | 3.92 | -0.11 | -9.00 | 9.61 | 9.61 | 17.48 |
Nucleolus | 1.00 | 0.90 | -0.44 | -5.49 | -5.27 | 1.16 | -3.45 | -2.29 | -2.46 | -1.43 | 1.13 | 0.74 | -1.73 | -4.57 | -4.04 |
Nucleus | -2.71 | -6.13 | -8.65 | -6.88 | -3.61 | -1.46 | -2.75 | -7.02 | -6.50 | 2.05 | -2.93 | -7.03 | -11.13 | -9.71 | -3.28 |
Peroxisomes | -0.30 | 1.63 | -1.59 | -1.57 | -1.65 | -0.77 | -2.01 | -3.25 | -3.16 | -2.67 | -0.43 | 2.06 | -1.97 | -1.95 | -2.05 |
Vacuole | -8.15 | -23.49 | -34.50 | -29.18 | -20.91 | 6.46 | -7.16 | -27.21 | -30.31 | 0.79 | -0.67 | -19.94 | -43.30 | -42.65 | -25.86 |
Vacuole Periphery | 5.91 | -10.06 | 5.44 | -0.69 | 17.61* | 13.81 | 0.77 | 12.42 | -1.58 | 2.75 | 14.19 | -8.73 | 12.45 | -2.13 | 20.09* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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