Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 5 | 14 | 11 | 7 | 25 | 7 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 6 | 4 | 14 | 9 | 13 | 10 | 7 | 24 | 15 | 12 | 2 | 1 | 2 | 7 | 8 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 313 | 440 | 210 | 174 | 178 | 210 | 204 | 162 | 108 | 145 | 69 | 92 | 149 | 164 | 181 | 396 | 283 | 337 | 292 | 434 | 198 | 102 | 202 | 197 |
Endoplasmic Reticulum | 0 | 12 | 6 | 4 | 16 | 23 | 36 | 44 | 23 | 41 | 15 | 26 | 3 | 5 | 3 | 10 | 17 | 13 | 4 | 5 | 12 | 4 | 12 | 8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 8 | 3 | 4 | 11 | 0 | 9 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 10 | 26 | 27 | 30 | 27 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 1 | 3 | 4 | 3 | 1 | 9 | 3 | 8 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 4 | 2 | 0 | 3 | 1 |
Unique Cell Count | 313 | 452 | 217 | 181 | 205 | 244 | 268 | 222 | 157 | 241 | 124 | 162 | 152 | 169 | 181 | 407 | 287 | 339 | 301 | 451 | 223 | 110 | 227 | 214 |
Labelled Cell Count | 313 | 456 | 223 | 184 | 213 | 250 | 300 | 246 | 178 | 286 | 142 | 195 | 154 | 172 | 186 | 418 | 311 | 354 | 301 | 451 | 223 | 110 | 227 | 214 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 66.3 | 88.4 | 62.3 | 52.8 | 38.1 | 28.6 | 23.2 | 22.5 | 18.4 | 19.4 | 17.2 | 17.7 | 69.3 | 64.9 | 63.8 | 112.6 | 113.7 | 116.5 | 75.2 | 74.5 | 76.9 |
Std Deviation (1e-4) | 11.3 | 17.8 | 14.0 | 12.9 | 9.4 | 6.9 | 5.3 | 4.7 | 4.3 | 4.3 | 4.3 | 4.5 | 15.0 | 13.2 | 11.6 | 26.0 | 26.4 | 28.8 | 16.4 | 17.8 | 22.2 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.71 | -1.12 | -1.43 | -1.47 | -1.76 | -1.68 | -1.85 | -1.81 | 0.15 | 0.06 | 0.04 | 0.85 | 0.87 | 0.9 | 0.27 | 0.26 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.4 | 3.3 | 0 | 4.9 | 0 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.0 | 0.6 | 1.2 | 1.0 | 0.9 | 3.1 | 3.4 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | -3.8 | -4.0 | -6.4 | -6.9 | -7.5 | -9.4 | -9.5 | -9.6 | 0.7 | 0.2 | 2.4 | 0.4 | 1.4 | 2.4 |
Endoplasmic Reticulum | 0 | 2.3 | 2.9 | 4.2 | 5.6 | 4.2 | 5.0 | 3.4 | 4.6 | 0 | 0 | 0 | -0.2 | 1.7 | 0.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.0 | 0 | 6.2 | 5.1 | 7.6 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 68.5339 | 96.9746 | 84.2057 | 84.6772 | 64.6495 | 90.48 | 69.1499 | 94.619 | 82.3662 | 73.7829 | 76.914 | 81.3087 | 74.0434 | 96.4109 | 93.1654 | 91.0946 | 71.3247 | 73.0777 |
Actin | 0.0069 | 0.0002 | 0.0021 | 0.0004 | 0.0019 | 0.001 | 0.0052 | 0.0002 | 0.0108 | 0.0013 | 0.0017 | 0.0018 | 0.0134 | 0.0008 | 0.0037 | 0.0204 | 0.007 | 0.015 |
Bud | 0.0021 | 0.0006 | 0.0013 | 0.0003 | 0.001 | 0.0004 | 0.0013 | 0.0002 | 0.0007 | 0.0011 | 0.0005 | 0.0014 | 0.0026 | 0.002 | 0.0026 | 0.0013 | 0.0028 | 0.0013 |
Bud Neck | 0.0007 | 0.0003 | 0.0009 | 0.0004 | 0.0007 | 0.0028 | 0.0007 | 0.0004 | 0.0012 | 0.0004 | 0.0005 | 0.0017 | 0.0029 | 0.0008 | 0.0005 | 0.0007 | 0.001 | 0.0019 |
Bud Periphery | 0.0028 | 0.0007 | 0.0011 | 0.0003 | 0.0006 | 0.0005 | 0.0028 | 0.0002 | 0.0006 | 0.002 | 0.0023 | 0.0022 | 0.0041 | 0.0007 | 0.0012 | 0.0007 | 0.0013 | 0.0009 |
Bud Site | 0.0016 | 0.0004 | 0.0027 | 0.0002 | 0.0009 | 0.0001 | 0.0006 | 0.0002 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0036 | 0.0027 | 0.0009 | 0.0019 | 0.0016 | 0.0002 |
Cell Periphery | 0.0109 | 0.0083 | 0.0156 | 0.0025 | 0.0031 | 0.0058 | 0.0062 | 0.0069 | 0.0033 | 0.0584 | 0.002 | 0.0038 | 0.0147 | 0.0091 | 0.0055 | 0.0349 | 0.0013 | 0.0013 |
Cytoplasm | 0.7048 | 0.9349 | 0.8146 | 0.8062 | 0.7591 | 0.8389 | 0.7189 | 0.9562 | 0.8549 | 0.6016 | 0.8637 | 0.7875 | 0.6593 | 0.8954 | 0.8395 | 0.7108 | 0.7623 | 0.7492 |
Cytoplasmic Foci | 0.0187 | 0.0007 | 0.0108 | 0.0026 | 0.0323 | 0.0031 | 0.0089 | 0.001 | 0.0047 | 0.0031 | 0.0105 | 0.0067 | 0.0225 | 0.0063 | 0.0117 | 0.0081 | 0.0434 | 0.0259 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0452 | 0.0135 | 0.0107 | 0.006 | 0.0202 | 0.0839 | 0.067 | 0.0146 | 0.0157 | 0.0263 | 0.0186 | 0.0441 | 0.0363 | 0.0073 | 0.0082 | 0.0164 | 0.0037 | 0.0258 |
Endosome | 0.0233 | 0.0011 | 0.0074 | 0.0122 | 0.0336 | 0.0079 | 0.0207 | 0.0017 | 0.0102 | 0.04 | 0.0121 | 0.0331 | 0.0144 | 0.0025 | 0.0043 | 0.0095 | 0.0288 | 0.0202 |
Golgi | 0.004 | 0.0002 | 0.0017 | 0.009 | 0.0154 | 0.0024 | 0.0031 | 0.0006 | 0.002 | 0.0021 | 0.0012 | 0.0101 | 0.0039 | 0.0009 | 0.0012 | 0.0022 | 0.005 | 0.0028 |
Lipid Particles | 0.0075 | 0.0009 | 0.006 | 0.0029 | 0.0194 | 0.0017 | 0.0039 | 0.0007 | 0.0031 | 0.0057 | 0.0026 | 0.0008 | 0.0091 | 0.0023 | 0.0044 | 0.0017 | 0.0289 | 0.0012 |
Mitochondria | 0.0074 | 0.001 | 0.0064 | 0.0053 | 0.0085 | 0.0015 | 0.0183 | 0.0004 | 0.0082 | 0.0293 | 0.0131 | 0.0241 | 0.0209 | 0.0069 | 0.0066 | 0.0016 | 0.0023 | 0.0032 |
None | 0.0063 | 0.0004 | 0.003 | 0.0002 | 0.0098 | 0.0022 | 0.0029 | 0.0001 | 0.0028 | 0.0022 | 0.0142 | 0.0028 | 0.0227 | 0.0085 | 0.0244 | 0.0005 | 0.005 | 0.0164 |
Nuclear Periphery | 0.0196 | 0.0032 | 0.0038 | 0.0044 | 0.0108 | 0.0121 | 0.0199 | 0.0017 | 0.0089 | 0.0135 | 0.0065 | 0.0102 | 0.0154 | 0.003 | 0.0051 | 0.006 | 0.0171 | 0.0722 |
Nucleolus | 0.0065 | 0.0003 | 0.0027 | 0.0003 | 0.0052 | 0.0007 | 0.0045 | 0 | 0.0025 | 0.0026 | 0.0008 | 0.0013 | 0.0143 | 0.0016 | 0.0026 | 0.0005 | 0.0011 | 0.0011 |
Nucleus | 0.0093 | 0.0025 | 0.0039 | 0.0026 | 0.006 | 0.0035 | 0.007 | 0.0009 | 0.0043 | 0.0057 | 0.0026 | 0.0043 | 0.0103 | 0.0033 | 0.0035 | 0.004 | 0.0037 | 0.0245 |
Peroxisomes | 0.0019 | 0 | 0.0023 | 0.0001 | 0.0011 | 0 | 0.0014 | 0 | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0088 | 0.0003 | 0.0025 | 0.0023 | 0.0096 | 0.0003 |
Punctate Nuclear | 0.0031 | 0 | 0.0006 | 0 | 0.0053 | 0.0001 | 0.0016 | 0 | 0.0021 | 0.0001 | 0.009 | 0.0001 | 0.003 | 0.0009 | 0.0011 | 0.0011 | 0.0095 | 0.0051 |
Vacuole | 0.0984 | 0.0294 | 0.0901 | 0.124 | 0.0517 | 0.0241 | 0.077 | 0.0131 | 0.0529 | 0.1494 | 0.0272 | 0.0383 | 0.1012 | 0.0398 | 0.0574 | 0.1571 | 0.0541 | 0.0228 |
Vacuole Periphery | 0.0187 | 0.0015 | 0.0122 | 0.0199 | 0.0131 | 0.0068 | 0.0279 | 0.0009 | 0.0097 | 0.0536 | 0.0098 | 0.0252 | 0.0156 | 0.0049 | 0.0128 | 0.0184 | 0.0106 | 0.0086 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2008.3897 | 2408.8344 | 2694.0296 | 2023.145 | 2619.0241 | 2346.8222 | 2002.9589 | 1863.4974 | 2241.965 | 1786.0153 |
Translational Efficiency | 1.4418 | 1.262 | 1.1842 | 1.6672 | 1.3674 | 1.2329 | 1.2777 | 1.5031 | 1.1475 | 1.5046 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1691 | 1448 | 346 | 195 | 1729 | 1412 | 771 | 1369 | 3420 | 2860 | 1117 | 1564 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 4058.91 | 4962.38 | 6124.11 | 6207.05 | 4259.51 | 4738.10 | 5286.22 | 4564.10 | 4160.32 | 4851.65 | 5545.76 | 4768.94 |
Standard Deviation | 966.65 | 984.62 | 1127.43 | 1519.23 | 778.94 | 971.66 | 1260.70 | 1318.80 | 882.51 | 984.65 | 1280.97 | 1450.77 |
Intensity Change Log 2 | — | 0.289940 | 0.593408 | 0.612815 | — | 0.153621 | 0.311549 | 0.099643 | — | 0.221747 | 0.455959 | 0.372791 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000038 | 0.000064 | 0.000216 | 0.000349 | 0.000047 | 0.000067 | 0.000222 | 0.000257 | 0.000042 | 0.000066 | 0.000220 | 0.000268 |
Bud Neck | 0.000161 | 0.000390 | 0.004299 | 0.003590 | 0.000233 | 0.000254 | 0.004815 | 0.001413 | 0.000198 | 0.000323 | 0.004655 | 0.001684 |
Bud Site | 0.001677 | 0.003346 | 0.010235 | 0.013943 | 0.002178 | 0.002394 | 0.013787 | 0.011720 | 0.001930 | 0.002876 | 0.012687 | 0.011997 |
Cell Periphery | 0.010061 | 0.013241 | 0.035543 | 0.075816 | 0.009711 | 0.009493 | 0.016226 | 0.017561 | 0.009884 | 0.011391 | 0.022209 | 0.024825 |
Cytoplasm | 0.746422* | 0.743779* | 0.478891* | 0.381939* | 0.698235* | 0.803692* | 0.350092* | 0.718926* | 0.722061* | 0.773359* | 0.389988* | 0.676910* |
Cytoplasmic Foci | 0.008602 | 0.009857 | 0.024216 | 0.010439 | 0.011598 | 0.008518 | 0.013222 | 0.006153 | 0.010117 | 0.009196 | 0.016628 | 0.006687 |
Eisosomes | 0.000001 | 0.000001 | 0.000007 | 0.000011 | 0.000001 | 0.000001 | 0.000005 | 0.000004 | 0.000001 | 0.000001 | 0.000005 | 0.000004 |
Endoplasmic Reticulum | 0.001333 | 0.000834 | 0.000591 | 0.000355 | 0.001940 | 0.001041 | 0.001465 | 0.000456 | 0.001640 | 0.000936 | 0.001194 | 0.000444 |
Endosome | 0.000630 | 0.000789 | 0.001783 | 0.000712 | 0.000796 | 0.000692 | 0.002856 | 0.001365 | 0.000714 | 0.000741 | 0.002524 | 0.001284 |
Golgi | 0.056176 | 0.063378 | 0.086826 | 0.032191 | 0.075842 | 0.049580 | 0.061935 | 0.017248 | 0.066118 | 0.056565 | 0.069646 | 0.019111 |
Lipid Particles | 0.007789 | 0.012919 | 0.029152 | 0.041795 | 0.009985 | 0.010569 | 0.024779 | 0.015839 | 0.008899 | 0.011759 | 0.026133 | 0.019075 |
Mitochondria | 0.000191 | 0.000186 | 0.000158 | 0.000097 | 0.000193 | 0.000359 | 0.000264 | 0.000449 | 0.000192 | 0.000272 | 0.000231 | 0.000405 |
Mitotic Spindle | 0.000624 | 0.001175 | 0.005256 | 0.001361 | 0.001241 | 0.001344 | 0.003193 | 0.000844 | 0.000936 | 0.001258 | 0.003832 | 0.000908 |
None | 0.001572 | 0.001693 | 0.001814 | 0.000791 | 0.001370 | 0.001952 | 0.001671 | 0.002767 | 0.001470 | 0.001821 | 0.001716 | 0.002520 |
Nuclear Periphery | 0.000411 | 0.000525 | 0.002680 | 0.001114 | 0.000666 | 0.000304 | 0.002508 | 0.000253 | 0.000540 | 0.000416 | 0.002562 | 0.000360 |
Nuclear Periphery Foci | 0.011207 | 0.016138 | 0.023980 | 0.009440 | 0.014594 | 0.010848 | 0.026459 | 0.005156 | 0.012919 | 0.013526 | 0.025691 | 0.005690 |
Nucleolus | 0.000076 | 0.000058 | 0.000119 | 0.000136 | 0.000047 | 0.000055 | 0.000272 | 0.000185 | 0.000062 | 0.000057 | 0.000225 | 0.000179 |
Nucleus | 0.004698 | 0.004208 | 0.017767 | 0.011436 | 0.003066 | 0.004005 | 0.015358 | 0.008955 | 0.003873 | 0.004108 | 0.016104 | 0.009264 |
Peroxisomes | 0.000031 | 0.000023 | 0.000028 | 0.000044 | 0.000019 | 0.000018 | 0.000051 | 0.000079 | 0.000025 | 0.000021 | 0.000044 | 0.000075 |
Vacuole | 0.061454 | 0.066373 | 0.192532 | 0.356268* | 0.061464 | 0.047448 | 0.378792* | 0.165414 | 0.061459 | 0.057030 | 0.321096* | 0.189209 |
Vacuole Periphery | 0.086845 | 0.061023 | 0.083905 | 0.058172 | 0.106774* | 0.047365 | 0.082028 | 0.024958 | 0.096920 | 0.054280 | 0.082610 | 0.029099 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.32 | -8.97 | -4.38 | -3.99 | -1.82 | -1.46 | -7.04 | -3.10 | -2.80 | 0.23 | -2.99 | -9.19 | -3.76 | -3.37 | -0.20 |
Bud Neck | -5.78 | -7.98 | -5.07 | -4.80 | 0.13 | -0.63 | -6.78 | -6.76 | -6.66 | 5.25 | -4.78 | -8.48 | -8.48 | -7.88 | 5.72 |
Bud Site | -3.97 | -4.60 | -3.74 | -3.25 | -1.27 | -0.44 | -6.68 | -6.47 | -6.51 | 1.02 | -2.98 | -8.07 | -7.58 | -6.92 | 0.30 |
Cell Periphery | -2.78 | -8.22 | -8.29 | -7.79 | -4.15 | 0.17 | -3.11 | -4.60 | -4.49 | -0.96 | -1.99 | -7.61 | -8.18 | -7.01 | -0.77 |
Cytoplasm | 0.38 | 13.59 | 14.11 | 13.82 | 2.50 | -9.74 | 24.52 | -0.78 | 8.59 | -24.61 | -6.54 | 29.08 | 5.44 | 10.44 | -21.25 |
Cytoplasmic Foci | -1.43 | -6.27 | -1.99 | -1.28 | 4.33 | 3.31 | -2.50 | 5.24 | 2.24 | 6.26 | 1.30 | -6.57 | 3.59 | 2.56 | 8.43 |
Eisosomes | -3.55 | -7.65 | -4.86 | -4.68 | -1.60 | -0.32 | -7.40 | -5.28 | -5.13 | 2.09 | -2.36 | -10.44 | -7.11 | -6.68 | 1.89 |
Endoplasmic Reticulum | 3.57 | 4.85 | 7.47 | 5.93 | 2.31 | 2.98 | 1.86 | 5.32 | 3.54 | 3.25 | 4.21 | 2.59 | 7.12 | 4.45 | 3.38 |
Endosome | -2.71 | -5.62 | -1.18 | 0.57 | 4.79 | 1.59 | -8.89 | -2.34 | -2.74 | 4.23 | -0.69 | -10.57 | -2.70 | -2.55 | 4.30 |
Golgi | -2.04 | -4.87 | 7.15 | 8.85 | 9.33 | 7.39 | 5.48 | 20.96 | 13.01 | 12.73 | 3.74 | 0.80 | 23.49 | 19.33 | 16.82 |
Lipid Particles | -4.65 | -9.86 | -6.30 | -5.20 | -1.52 | -0.54 | -7.84 | -3.58 | -3.12 | 4.07 | -3.54 | -12.26 | -6.72 | -4.53 | 4.08 |
Mitochondria | 0.15 | 0.97 | 3.60 | 2.19 | 3.00 | -1.30 | -2.97 | -1.95 | -0.71 | -1.21 | -1.18 | -2.53 | -1.87 | -1.18 | -1.35 |
Mitotic Spindle | -4.02 | -3.62 | -1.70 | -0.43 | 2.95 | -0.18 | -3.02 | 0.89 | 0.92 | 3.54 | -1.22 | -4.84 | -0.21 | 1.00 | 4.74 |
None | -0.87 | -1.37 | 5.91 | 5.80 | 5.67 | -1.75 | -1.04 | -1.73 | -0.89 | -1.18 | -1.96 | -1.16 | -1.47 | -0.91 | -1.01 |
Nuclear Periphery | -1.65 | -8.40 | -5.87 | -5.62 | 5.77 | 6.35 | -3.83 | 6.45 | 0.70 | 4.63 | 2.62 | -5.97 | 3.01 | 0.98 | 6.43 |
Nuclear Periphery Foci | -4.58 | -7.63 | 1.99 | 5.79 | 8.41 | 3.60 | -5.45 | 11.42 | 6.80 | 10.49 | -0.89 | -8.33 | 12.01 | 11.34 | 13.42 |
Nucleolus | 1.12 | -2.78 | -3.62 | -5.62 | -1.57 | -0.68 | -7.10 | -6.74 | -5.71 | 2.28 | 0.49 | -7.11 | -6.28 | -6.45 | 1.51 |
Nucleus | 0.59 | -6.56 | -4.99 | -5.23 | 1.70 | -1.67 | -8.29 | -10.03 | -8.44 | 4.59 | -0.66 | -10.06 | -10.32 | -9.98 | 5.27 |
Peroxisomes | 0.66 | 0.18 | -1.13 | -2.91 | -1.73 | 0.27 | -4.68 | -5.45 | -5.45 | -2.12 | 0.70 | -2.71 | -4.58 | -5.49 | -2.77 |
Vacuole | -1.29 | -11.58 | -15.41 | -15.05 | -7.14 | 3.52 | -30.17 | -16.94 | -18.85 | 17.20 | 1.34 | -30.84 | -21.40 | -21.75 | 12.27 |
Vacuole Periphery | 5.89 | 0.93 | 5.14 | 1.47 | 3.44 | 13.02 | 6.95 | 20.47 | 8.23 | 12.99* | 13.43 | 5.78 | 23.67 | 11.43 | 14.17 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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