Standard name | |
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Human Ortholog | |
Description | Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 146 | 90 | 170 | 150 | 148 | 230 | 196 | 152 | 150 | 186 | 158 | 206 | 61 | 88 | 89 | 123 | 280 | 130 | 38 | 44 | 51 | 27 | 31 | 40 |
Bud | 25 | 30 | 31 | 38 | 32 | 36 | 42 | 32 | 33 | 40 | 35 | 54 | 5 | 15 | 11 | 11 | 29 | 12 | 2 | 4 | 4 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 16 | 5 | 5 | 6 | 7 |
Bud Site | 2 | 5 | 4 | 3 | 2 | 3 | 2 | 0 | 1 | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 3 | 1 | – | – | – | – | – | – |
Cell Periphery | 126 | 71 | 162 | 116 | 92 | 150 | 154 | 102 | 136 | 159 | 128 | 166 | 85 | 158 | 209 | 191 | 394 | 241 | 190 | 98 | 237 | 188 | 247 | 327 |
Cytoplasm | 6 | 15 | 15 | 16 | 11 | 12 | 17 | 9 | 9 | 5 | 12 | 8 | 8 | 21 | 20 | 47 | 72 | 33 | 0 | 2 | 1 | 0 | 1 | 2 |
Endoplasmic Reticulum | 6 | 9 | 8 | 10 | 23 | 60 | 48 | 82 | 38 | 64 | 44 | 52 | 5 | 10 | 9 | 16 | 45 | 17 | 2 | 3 | 6 | 1 | 5 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 1 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 1 | 3 | 1 | 5 | 19 | 1 | 4 | 2 | 5 | 2 | 4 | 1 | 0 | 31 | 30 | 0 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 118 | 81 | 197 | 186 | 207 | 339 | 426 | 413 | 334 | 549 | 421 | 582 | 10 | 12 | 4 | 32 | 50 | 20 | 5 | 4 | 6 | 10 | 11 | 12 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 9 | 8 | 5 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 37 | 2 | 84 | 68 | 65 | 77 | 93 | 52 | 35 | 51 | 22 | 32 | 0 | 1 | 0 | 13 | 29 | 16 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 6 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 3 | 2 | 1 | 4 | 10 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 18 | 0 | 1 | 0 | 6 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 5 | 0 | 1 | 1 | 2 |
Unique Cell Count | 233 | 160 | 307 | 251 | 292 | 435 | 537 | 545 | 395 | 624 | 472 | 668 | 116 | 218 | 252 | 329 | 688 | 342 | 254 | 185 | 317 | 239 | 315 | 415 |
Labelled Cell Count | 472 | 303 | 675 | 591 | 584 | 911 | 989 | 891 | 747 | 1066 | 827 | 1123 | 181 | 312 | 345 | 439 | 951 | 507 | 254 | 185 | 317 | 239 | 315 | 415 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 7.4 | 7.5 | 8.5 | 8.8 | 8.5 | 8.3 | 9.0 | 9.1 | 8.9 | 9.4 | 9.1 | 10.0 | 9.6 | 9.7 | 6.7 | 8.5 | 10.6 | 9.5 | 9.6 | 10.0 |
Std Deviation (1e-4) | 1.4 | 1.5 | 1.5 | 1.5 | 1.6 | 1.4 | 1.6 | 1.8 | 1.6 | 1.6 | 1.7 | 1.7 | 1.4 | 1.3 | 1.5 | 1.3 | 1.9 | 2.1 | 2.0 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.23 | 0.18 | 0.14 | 0.26 | 0.27 | 0.23 | 0.32 | 0.28 | 0.4 | 0.34 | 0.36 | -0.18 | 0.17 | 0.49 | 0.34 | 0.35 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.0 | -1.1 | -0.7 | -5.3 | -7.9 | -4.6 | -7.5 | -6.0 | -7.3 | -0.5 | -3.4 | -4.7 | -4.5 | -4.3 | -4.4 |
Bud | 1.8 | 0.3 | -0.9 | -1.1 | -2.3 | -0.8 | -2.0 | -1.3 | -1.0 | -1.9 | -1.3 | -2.6 | -3.4 | -3.6 | -3.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.5 | -5.3 | -5.0 | -7.0 | -10.3 | -4.9 | -8.2 | -7.2 | -8.6 | 3.8 | 4.6 | 7.5 | 1.3 | 1.3 | 4.6 |
Cytoplasm | 0.8 | -0.7 | -1.5 | -1.3 | -2.7 | -1.9 | -4.0 | -1.7 | -3.5 | 0.8 | 2.1 | 1.5 | 4.0 | 2.9 | 2.3 |
Endoplasmic Reticulum | 0.9 | 2.9 | 5.2 | 3.6 | 5.7 | 3.7 | 4.1 | 3.7 | 3.1 | 0 | 1.2 | 0.7 | 1.5 | 2.6 | 1.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 5.3 |
Mitochondria | 2.5 | 1.8 | 4.1 | 4.8 | 3.6 | 6.2 | 8.6 | 8.4 | 8.3 | -10.2 | -13.5 | -15.3 | -14.3 | -19.2 | -15.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.1 | -1.4 | -3.1 | -3.4 | -6.8 | -6.5 | -7.8 | -9.0 | -10.1 | -6.3 | -8.2 | -9.0 | -8.2 | -10.6 | -8.0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.5482 | 12.2593 | 9.9041 | 9.8343 | 10.0671 | 10.137 | 6.9195 | 8.8163 | 8.7863 | 8.8378 | 7.7125 | 8.7521 | 6.4533 | 8.9565 | 8.6315 | 7.8293 | 8.5518 | 8.0358 |
Actin | 0.0724 | 0.0185 | 0.045 | 0.0216 | 0.0149 | 0.0254 | 0.0562 | 0.1299 | 0.0189 | 0.0106 | 0.0461 | 0.0102 | 0.019 | 0.0014 | 0.0174 | 0.0929 | 0.0306 | 0.0289 |
Bud | 0.001 | 0.0016 | 0.0014 | 0.0042 | 0.002 | 0.0011 | 0.0018 | 0.0005 | 0.0014 | 0.0006 | 0.001 | 0.0006 | 0.0009 | 0.0006 | 0.001 | 0.0009 | 0.0015 | 0.0014 |
Bud Neck | 0.0094 | 0.0066 | 0.04 | 0.0329 | 0.0063 | 0.0491 | 0.0116 | 0.0059 | 0.0693 | 0.1129 | 0.0797 | 0.0456 | 0.0107 | 0.0114 | 0.1114 | 0.173 | 0.0461 | 0.0785 |
Bud Periphery | 0.0057 | 0.0102 | 0.0101 | 0.0283 | 0.014 | 0.0148 | 0.0059 | 0.0013 | 0.0056 | 0.0084 | 0.0044 | 0.0084 | 0.0053 | 0.0016 | 0.0042 | 0.0063 | 0.0092 | 0.0156 |
Bud Site | 0.0356 | 0.2404 | 0.0672 | 0.0151 | 0.0076 | 0.0034 | 0.052 | 0.2597 | 0.1614 | 0.009 | 0.0051 | 0.0044 | 0.058 | 0.5228 | 0.252 | 0.0174 | 0.0062 | 0.0094 |
Cell Periphery | 0.5853 | 0.5452 | 0.6607 | 0.5997 | 0.6582 | 0.7188 | 0.5956 | 0.5379 | 0.6309 | 0.755 | 0.3497 | 0.7748 | 0.8136 | 0.428 | 0.4692 | 0.5523 | 0.5895 | 0.691 |
Cytoplasm | 0.006 | 0.011 | 0.0068 | 0.012 | 0.005 | 0.0071 | 0.0061 | 0.0147 | 0.0093 | 0.0067 | 0.002 | 0.0048 | 0.0076 | 0.0157 | 0.0066 | 0.0033 | 0.0032 | 0.009 |
Cytoplasmic Foci | 0.0061 | 0.0003 | 0.0053 | 0.0008 | 0.0102 | 0.0008 | 0.0392 | 0.0003 | 0.0004 | 0.0004 | 0.0142 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0019 | 0.0573 | 0.0009 |
Eisosomes | 0.2075 | 0.145 | 0.0798 | 0.1913 | 0.1078 | 0.1546 | 0.1587 | 0.043 | 0.0908 | 0.0767 | 0.1721 | 0.1313 | 0.0751 | 0.015 | 0.0982 | 0.1191 | 0.1272 | 0.1125 |
Endoplasmic Reticulum | 0.0123 | 0.006 | 0.0119 | 0.0551 | 0.0429 | 0.0045 | 0.0013 | 0.0017 | 0.0013 | 0.0087 | 0.0031 | 0.0055 | 0.0012 | 0.0002 | 0.0015 | 0.0019 | 0.018 | 0.0099 |
Endosome | 0.0061 | 0.0001 | 0.0066 | 0.0011 | 0.0115 | 0.0012 | 0.0167 | 0 | 0.0001 | 0.0001 | 0.0437 | 0.0002 | 0 | 0 | 0.0022 | 0.002 | 0.0084 | 0.0189 |
Golgi | 0.0052 | 0.0004 | 0.0074 | 0.0036 | 0.0058 | 0.0018 | 0.0202 | 0.0004 | 0.0004 | 0.0009 | 0.0466 | 0.0008 | 0.0001 | 0 | 0.0023 | 0.0072 | 0.0166 | 0.0089 |
Lipid Particles | 0.005 | 0.0004 | 0.0088 | 0.0023 | 0.0072 | 0.0008 | 0.0032 | 0.0002 | 0.0003 | 0.0006 | 0.0174 | 0.0004 | 0.0002 | 0.0001 | 0.0041 | 0.0048 | 0.0126 | 0.0009 |
Mitochondria | 0.0175 | 0.0051 | 0.0136 | 0.0138 | 0.0436 | 0.0061 | 0.0056 | 0.0012 | 0.0033 | 0.0023 | 0.15 | 0.0066 | 0.0009 | 0.0002 | 0.0188 | 0.008 | 0.0446 | 0.0066 |
None | 0.0065 | 0.0008 | 0.0015 | 0.0014 | 0.0048 | 0.0019 | 0.0016 | 0.0004 | 0.0006 | 0.0009 | 0.0008 | 0.0008 | 0.0006 | 0.0004 | 0.0005 | 0.0007 | 0.0011 | 0.0008 |
Nuclear Periphery | 0.0043 | 0.0014 | 0.0058 | 0.0025 | 0.023 | 0.0014 | 0.0007 | 0.0005 | 0.0005 | 0.0009 | 0.0015 | 0.0006 | 0.0006 | 0.0002 | 0.0004 | 0.0005 | 0.0025 | 0.0016 |
Nucleolus | 0.0005 | 0.0004 | 0.0024 | 0.0009 | 0.0029 | 0.0004 | 0.0003 | 0.0001 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0007 | 0.0005 | 0.0003 |
Nucleus | 0.0008 | 0.0005 | 0.005 | 0.001 | 0.0095 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0005 | 0.0006 | 0.0004 |
Peroxisomes | 0.0049 | 0.0002 | 0.0034 | 0.0004 | 0.0054 | 0.0003 | 0.0197 | 0.0004 | 0.0005 | 0.0002 | 0.0571 | 0.0003 | 0.0002 | 0.0001 | 0.0008 | 0.0028 | 0.018 | 0.0004 |
Punctate Nuclear | 0.0017 | 0 | 0.0078 | 0.0001 | 0.005 | 0.0003 | 0.0004 | 0 | 0 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 |
Vacuole | 0.0053 | 0.0054 | 0.0083 | 0.0107 | 0.0092 | 0.0051 | 0.0026 | 0.0017 | 0.0038 | 0.0038 | 0.0023 | 0.0032 | 0.0052 | 0.0015 | 0.0063 | 0.0032 | 0.0047 | 0.0033 |
Vacuole Periphery | 0.0009 | 0.0003 | 0.0014 | 0.0012 | 0.003 | 0.0006 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0018 | 0.0004 | 0.0001 | 0 | 0.0011 | 0.0004 | 0.0014 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.6681 | 99.2973 | 80.3813 | 82.6616 | 55.8951 | 76.8578 | 104.8752 | 97.1425 | 72.2274 | 73.9424 |
Translational Efficiency | 0.6693 | 0.6076 | 0.7698 | 0.5026 | 0.6649 | 0.6678 | 0.5592 | 0.5145 | 0.5905 | 0.4451 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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