Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.05 0.05 0 0.1 0 0 0 0 0.07 0.17 0.21 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.08 0.05 0.09 0.4 0.26 0.18 0.3 0.27 0.43 0.23 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.9 0.91 0.93 0.91 0.86 0.79 0.83 0.73 0.81 0.82 0.77 0.82 0.91 0.79 0.72 0.86 0.9 0.9 0.87 0.85 0.87 0.74 0.67 0.56
Nuclear Periphery 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.08 0.11 0.09 0.11 0.13 0.12 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0 0.07 0.07 0.05 0.15 0.22 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 0 1 1 4 8 11 4 3 4 6 5 0 0 0 0 0 0 3 11 8 1 1 1
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 2 0 3 3 6 8 29 10 23 38 46 37 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 16 23 20 28 44 28 85 17 25 27 10 4 24 21 7 3 4 2 3 3 1 1 4
Endoplasmic Reticulum 0 0 4 0 1 2 1 0 0 0 0 0 0 1 0 3 4 1 0 0 0 2 0 1
Endosome 2 2 1 4 6 2 2 4 1 2 1 5 1 0 2 0 0 0 0 2 2 0 1 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Mitochondria 6 22 47 26 55 380 247 162 198 220 326 168 1 0 0 8 6 4 0 4 4 3 5 11
Nucleus 185 305 577 520 530 746 800 647 532 680 592 595 51 111 71 115 127 93 181 302 549 123 140 150
Nuclear Periphery 0 22 7 15 7 11 25 20 3 21 11 17 0 0 1 2 0 1 0 0 4 1 0 4
Nucleolus 1 7 10 11 13 53 78 97 60 89 99 86 1 4 0 6 2 2 0 1 12 3 4 11
Peroxisomes 0 0 2 1 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 4 3 1 2 6 0 1 1 2 0 2 4 0 2 0 5 2 4 0 2 3
Vac/Vac Membrane 9 11 8 17 27 29 16 54 15 7 9 8 0 5 4 8 5 3 13 23 29 25 45 67
Unique Cell Count 205 336 623 574 613 945 968 885 659 825 766 722 56 140 98 133 141 103 208 357 629 167 208 271
Labelled Cell Count 212 387 685 622 681 1285 1239 1091 852 1089 1118 933 58 147 105 149 149 108 208 357 629 167 208 271


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.2 5.6 5.4 5.3 4.3 4.7 6.1 5.0 4.9 4.5 5.1 6.3 6.3 6.5 9.0 8.5 8.7 5.9 5.9 6.1
Std Deviation (1e-4) 1.0 1.6 1.6 1.4 1.6 1.4 1.5 3.3 1.5 1.2 1.5 1.3 1.4 1.5 1.7 2.5 2.3 2.6 1.2 1.3 1.4
Intensity Change (Log2) – – – -0.04 -0.07 -0.36 -0.24 0.12 -0.16 -0.18 -0.3 -0.13 0.19 0.18 0.22 0.7 0.6 0.64 0.08 0.08 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1.3 3.8 4.7 0 5.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 0.8 0.9 -0.9 4.4 -1.1 -0.7 -0.2 -2.7 0 6.0 6.8 0.8 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.2 0.9 0 0 6.0 10.2 9.3 0 7.8 0 -3.4 -2.8 -0.6 -1.4 -1.3
Nucleus -1.3 -3.5 -7.3 -5.7 -9.5 -6.2 -5.7 -7.8 -5.6 -0.4 -4.8 -6.2 -2.3 -1.0 -0.8
Nuclear Periphery 1.9 0 0 0 1.6 0 1.9 0 1.7 0 0 0 0 0 0
Nucleolus 0.4 0.7 0 0 7.0 5.9 6.9 0 7.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 3.3 2.3 0.6 4.6 1.3 -0.8 -0.2 -0.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.031 2.4169 2.3021 1.6786 1.7469 2.2145 3.7285 3.9349 4.2526 3.4875 3.504 4.2126 2.5147 3.0961 2.5092 2.7832 1.1888 2.5164
Actin 0.0032 0 0 0 0.0116 0.0019 0.0123 0 0.0054 0.0002 0.0002 0.0051 0.0008 0 0.0109 0.0014 0 0
Bud 0.0002 0.0002 0 0 0.0002 0.0001 0.0005 0 0.0001 0 0 0.0001 0.0006 0 0.0002 0.0003 0 0.0001
Bud Neck 0.0004 0.0002 0 0.0001 0.0027 0.0008 0.0014 0 0.0003 0.0001 0.0004 0.0008 0.0005 0 0 0.0003 0.0001 0.0007
Bud Periphery 0.0003 0.0002 0 0 0.0003 0.0002 0.001 0 0.0003 0 0.0001 0.0005 0.0008 0 0.0002 0.0003 0 0.0001
Bud Site 0.0016 0.0048 0 0 0.0018 0.0001 0.002 0 0.0005 0 0 0.0002 0.0057 0 0.0004 0.0029 0 0
Cell Periphery 0.0001 0.0003 0 0 0.0001 0 0.0002 0 0.0001 0 0 0.0001 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0024 0.0005 0.0004 0 0.0004 0.0004 0.0075 0 0.0017 0.0011 0.0008 0.0003 0.0232 0.0001 0.001 0.0035 0.0001 0.0015
Cytoplasmic Foci 0.0045 0 0 0 0.0018 0.0001 0.0124 0 0.004 0.0006 0.0003 0.0018 0.0136 0 0.001 0.0053 0 0
Eisosomes 0.0001 0 0 0 0.0003 0 0.0002 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0011 0 0 0 0.0005 0.0003 0.0031 0 0.0014 0.0003 0.0003 0.0004 0.0021 0 0.0003 0.0009 0 0
Endosome 0.0062 0 0 0 0.0038 0.0008 0.0122 0 0.007 0.0009 0.0029 0.0042 0.0058 0 0.0009 0.0089 0 0
Golgi 0.001 0 0 0 0.0018 0.0001 0.003 0 0.0021 0.0007 0.0002 0.0016 0.0014 0 0.0004 0.0014 0 0
Lipid Particles 0.004 0 0 0 0.0039 0.0001 0.012 0 0.0028 0.0219 0.0001 0.0036 0.0075 0 0.0001 0.0022 0 0
Mitochondria 0.0012 0.0001 0.0001 0.0001 0.0009 0.0004 0.0055 0.0001 0.0075 0.0009 0.0009 0.0042 0.0031 0.0002 0.0024 0.003 0.0001 0.0002
None 0.0041 0.0001 0.0013 0 0.0014 0.0024 0.0041 0 0.0008 0.0032 0.0003 0.0003 0.0067 0.0001 0.0006 0.0028 0 0
Nuclear Periphery 0.0094 0.0031 0.0021 0.0012 0.0057 0.0042 0.0236 0.0031 0.0115 0.017 0.0119 0.0052 0.0082 0.0013 0.0017 0.0098 0.0005 0.001
Nucleolus 0.0153 0.019 0.0093 0.0118 0.0292 0.0213 0.0129 0.0078 0.0064 0.005 0.0229 0.0187 0.0148 0.0066 0.0112 0.0106 0.0045 0.0164
Nucleus 0.9304 0.9695 0.9847 0.9864 0.9119 0.9623 0.8617 0.9871 0.9385 0.933 0.9544 0.9474 0.878 0.9911 0.9671 0.9169 0.9945 0.9794
Peroxisomes 0.0015 0 0 0 0.0013 0 0.0094 0 0.0017 0.0007 0 0.0022 0.0058 0 0.0009 0.0009 0 0
Punctate Nuclear 0.0087 0.0009 0.0016 0.0003 0.0194 0.0037 0.0101 0.0017 0.0051 0.0134 0.0033 0.0026 0.0188 0.0002 0.0004 0.026 0.0001 0.0002
Vacuole 0.0032 0.0008 0.0002 0 0.0005 0.0005 0.0029 0 0.0012 0.0003 0.0004 0.0005 0.0016 0.0001 0.0002 0.0015 0 0.0001
Vacuole Periphery 0.0012 0.0001 0.0001 0 0.0004 0.0002 0.0018 0.0001 0.0015 0.0007 0.0005 0.0005 0.0009 0.0001 0.0002 0.0008 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.3211 35.1946 22.6796 33.323 34.2541 42.455 31.6053 28.758 29.0331 45.608
Translational Efficiency 1.1949 1.0807 1.4309 1.0498 1.38 1.4876 1.2266 1.1834 1.2567 1.053

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
543 1300 2615 286 1682 1940 1647 7 2225 3240 4262 293

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 751.72 824.63 940.92 1017.88 743.36 805.05 991.64 977.51 745.40 812.91 960.52 1016.92
Standard Deviation 109.58 121.72 136.19 223.79 107.90 119.92 165.96 141.81 108.37 121.03 150.45 222.27
Intensity Change Log 2 — 0.133552 0.323877 0.437300 — 0.115017 0.415755 0.395050 — 0.124366 0.370291 0.416448

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000310 0.000310 0.000427 0.002753 0.000262 0.000432 0.000419 0.000305 0.000274 0.000383 0.000424 0.002695
Bud Neck 0.002995 0.008738 0.018433 0.027182 0.004177 0.010852 0.024673 0.008757 0.003889 0.010004 0.020844 0.026742
Bud Site 0.000796 0.001811 0.001901 0.070100 0.000830 0.007152 0.001521 0.000902 0.000822 0.005009 0.001754 0.068447
Cell Periphery 0.000097 0.000166 0.000225 0.002167 0.000142 0.000295 0.000142 0.000057 0.000131 0.000243 0.000193 0.002117
Cytoplasm 0.038960 0.020849 0.006777 0.070877 0.016358 0.076226 0.004185 0.005551 0.021874 0.054007 0.005775 0.069317
Cytoplasmic Foci 0.004403 0.001542 0.000096 0.035462 0.001387 0.008292 0.000103 0.000309 0.002123 0.005584 0.000098 0.034622
Eisosomes 0.000014 0.000015 0.000025 0.000058 0.000014 0.000027 0.000025 0.000029 0.000014 0.000022 0.000025 0.000057
Endoplasmic Reticulum 0.001883 0.004357 0.003748 0.005878 0.003408 0.004080 0.003126 0.006202 0.003036 0.004191 0.003507 0.005886
Endosome 0.000406 0.000816 0.000428 0.018296 0.000557 0.001872 0.000412 0.000431 0.000520 0.001448 0.000422 0.017869
Golgi 0.000085 0.000581 0.000073 0.016054 0.000265 0.000834 0.000125 0.000017 0.000221 0.000732 0.000093 0.015671
Lipid Particles 0.000298 0.000399 0.000085 0.001510 0.000400 0.001997 0.000060 0.000404 0.000375 0.001356 0.000075 0.001483
Mitochondria 0.002708 0.001200 0.001107 0.010761 0.002015 0.004635 0.001329 0.000513 0.002185 0.003257 0.001193 0.010516
Mitotic Spindle 0.001582 0.002143 0.003242 0.041597 0.001977 0.009762 0.005973 0.000022 0.001880 0.006705 0.004298 0.040604
None 0.007616 0.006282 0.006318 0.008150 0.008248 0.010610 0.001916 0.000224 0.008094 0.008873 0.004617 0.007960
Nuclear Periphery 0.001127 0.001844 0.000670 0.001859 0.000883 0.001549 0.000784 0.001279 0.000942 0.001668 0.000714 0.001845
Nuclear Periphery Foci 0.000156 0.001246 0.000116 0.002295 0.000812 0.000478 0.000159 0.000031 0.000652 0.000786 0.000133 0.002241
Nucleolus 0.005658 0.010519 0.004365 0.007999 0.012578 0.006455 0.003005 0.000290 0.010889 0.008086 0.003839 0.007815
Nucleus 0.927290* 0.934976* 0.950430* 0.597906* 0.943752* 0.845226* 0.949654* 0.959472* 0.939735* 0.881237* 0.950130* 0.606544*
Peroxisomes 0.000979 0.000256 0.000069 0.000887 0.000620 0.001543 0.000075 0.000058 0.000708 0.001027 0.000071 0.000868
Vacuole 0.002354 0.001669 0.001333 0.053098 0.001068 0.007090 0.001911 0.015058 0.001382 0.004915 0.001557 0.052190
Vacuole Periphery 0.000283 0.000279 0.000131 0.025108 0.000246 0.000593 0.000403 0.000089 0.000255 0.000467 0.000237 0.024510

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.02 -0.99 -6.69 -7.77 -7.19 NaN NaN NaN NaN NaN -1.59 -3.54 -7.81 -7.50 -7.19
Bud Neck -3.17 -12.07 -7.71 -6.21 -2.01 NaN NaN NaN NaN NaN -7.22 -23.01 -8.37 -6.03 -0.79
Bud Site -1.33 -3.13 -5.81 -5.68 -5.65 NaN NaN NaN NaN NaN -6.04 -5.67 -5.83 -5.18 -5.66
Cell Periphery -2.52 -2.28 -4.53 -4.27 -4.15 NaN NaN NaN NaN NaN -4.72 -1.29 -4.40 -3.95 -4.25
Cytoplasm 3.29 6.06 -2.81 -5.48 -7.32 NaN NaN NaN NaN NaN -10.44 8.18 -5.35 -1.56 -7.41
Cytoplasmic Foci 2.01 2.84 -2.77 -4.02 -4.41 NaN NaN NaN NaN NaN -4.17 4.21 -3.78 -2.95 -4.41
Eisosomes 0.53 -3.47 -8.90 -9.72 -8.06 NaN NaN NaN NaN NaN -6.28 -9.20 -9.95 -8.65 -8.19
Endoplasmic Reticulum -5.83 -6.72 -4.54 -2.26 -2.89 NaN NaN NaN NaN NaN -4.39 -2.81 -3.83 -2.66 -3.30
Endosome -1.15 0.76 -3.18 -3.03 -3.26 NaN NaN NaN NaN NaN -4.94 1.21 -3.18 -2.68 -3.27
Golgi -1.13 0.01 -3.26 -2.90 -3.26 NaN NaN NaN NaN NaN -1.83 1.16 -3.18 -2.85 -3.25
Lipid Particles -0.54 3.87 -3.23 -2.89 -4.50 NaN NaN NaN NaN NaN -4.04 4.46 -3.05 0.83 -4.52
Mitochondria 0.73 0.71 -1.75 -3.29 -3.28 NaN NaN NaN NaN NaN -1.30 1.12 -2.57 -2.01 -3.19
Mitotic Spindle -0.45 -3.57 -5.48 -5.43 -4.96 NaN NaN NaN NaN NaN -3.94 -4.91 -5.47 -4.97 -4.90
None 0.60 0.91 -0.83 -1.52 -1.87 NaN NaN NaN NaN NaN -0.57 3.82 -0.45 -0.06 -2.84
Nuclear Periphery -1.38 0.66 -3.26 -2.43 -6.88 NaN NaN NaN NaN NaN -3.69 0.16 -6.07 -3.66 -6.74
Nuclear Periphery Foci -2.26 1.26 -4.43 -0.29 -4.86 NaN NaN NaN NaN NaN 0.02 2.39 -1.61 -1.87 -4.70
Nucleolus -2.84 0.13 -1.35 0.67 -1.59 NaN NaN NaN NaN NaN 2.45 6.09 0.82 -0.37 -1.83
Nucleus -1.34 -2.49 12.09 13.39 14.04 NaN NaN NaN NaN NaN 11.41 -0.68 13.66 10.65 13.92
Peroxisomes 0.87 1.10 -0.13 -2.79 -3.47 NaN NaN NaN NaN NaN -0.98 2.11 -0.84 0.01 -3.44
Vacuole 1.58 1.84 -7.33 -7.66 -7.70 NaN NaN NaN NaN NaN -6.22 -0.48 -7.77 -7.28 -7.75
Vacuole Periphery -0.07 1.20 -2.97 -2.97 -3.03 NaN NaN NaN NaN NaN -2.31 0.24 -2.98 -2.90 -2.99
Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rfc5

Rfc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available