Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.05 0.05 0 0.1 0 0 0 0 0.07 0.17 0.21 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.08 0.05 0.09 0.4 0.26 0.18 0.3 0.27 0.43 0.23 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.9 0.91 0.93 0.91 0.86 0.79 0.83 0.73 0.81 0.82 0.77 0.82 0.91 0.79 0.72 0.86 0.9 0.9 0.87 0.85 0.87 0.74 0.67 0.56
Nuclear Periphery 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.08 0.11 0.09 0.11 0.13 0.12 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0 0.07 0.07 0.05 0.15 0.22 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 0 1 1 4 8 11 4 3 4 6 5 0 0 0 0 0 0 3 11 8 1 1 1
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 2 0 3 3 6 8 29 10 23 38 46 37 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 16 23 20 28 44 28 85 17 25 27 10 4 24 21 7 3 4 2 3 3 1 1 4
Endoplasmic Reticulum 0 0 4 0 1 2 1 0 0 0 0 0 0 1 0 3 4 1 0 0 0 2 0 1
Endosome 2 2 1 4 6 2 2 4 1 2 1 5 1 0 2 0 0 0 0 2 2 0 1 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Mitochondria 6 22 47 26 55 380 247 162 198 220 326 168 1 0 0 8 6 4 0 4 4 3 5 11
Nucleus 185 305 577 520 530 746 800 647 532 680 592 595 51 111 71 115 127 93 181 302 549 123 140 150
Nuclear Periphery 0 22 7 15 7 11 25 20 3 21 11 17 0 0 1 2 0 1 0 0 4 1 0 4
Nucleolus 1 7 10 11 13 53 78 97 60 89 99 86 1 4 0 6 2 2 0 1 12 3 4 11
Peroxisomes 0 0 2 1 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 4 3 1 2 6 0 1 1 2 0 2 4 0 2 0 5 2 4 0 2 3
Vac/Vac Membrane 9 11 8 17 27 29 16 54 15 7 9 8 0 5 4 8 5 3 13 23 29 25 45 67
Unique Cell Count 205 336 623 574 613 945 968 885 659 825 766 722 56 140 98 133 141 103 208 357 629 167 208 271
Labelled Cell Count 212 387 685 622 681 1285 1239 1091 852 1089 1118 933 58 147 105 149 149 108 208 357 629 167 208 271


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.2 5.6 5.4 5.3 4.3 4.7 6.1 5.0 4.9 4.5 5.1 6.3 6.3 6.5 9.0 8.5 8.7 5.9 5.9 6.1
Std Deviation (1e-4) 1.0 1.6 1.6 1.4 1.6 1.4 1.5 3.3 1.5 1.2 1.5 1.3 1.4 1.5 1.7 2.5 2.3 2.6 1.2 1.3 1.4
Intensity Change (Log2) -0.04 -0.07 -0.36 -0.24 0.12 -0.16 -0.18 -0.3 -0.13 0.19 0.18 0.22 0.7 0.6 0.64 0.08 0.08 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1.3 3.8 4.7 0 5.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 0.8 0.9 -0.9 4.4 -1.1 -0.7 -0.2 -2.7 0 6.0 6.8 0.8 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.2 0.9 0 0 6.0 10.2 9.3 0 7.8 0 -3.4 -2.8 -0.6 -1.4 -1.3
Nucleus -1.3 -3.5 -7.3 -5.7 -9.5 -6.2 -5.7 -7.8 -5.6 -0.4 -4.8 -6.2 -2.3 -1.0 -0.8
Nuclear Periphery 1.9 0 0 0 1.6 0 1.9 0 1.7 0 0 0 0 0 0
Nucleolus 0.4 0.7 0 0 7.0 5.9 6.9 0 7.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 3.3 2.3 0.6 4.6 1.3 -0.8 -0.2 -0.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.031 2.4169 2.3021 1.6786 1.7469 2.2145 3.7285 3.9349 4.2526 3.4875 3.504 4.2126 2.5147 3.0961 2.5092 2.7832 1.1888 2.5164
Actin 0.0032 0 0 0 0.0116 0.0019 0.0123 0 0.0054 0.0002 0.0002 0.0051 0.0008 0 0.0109 0.0014 0 0
Bud 0.0002 0.0002 0 0 0.0002 0.0001 0.0005 0 0.0001 0 0 0.0001 0.0006 0 0.0002 0.0003 0 0.0001
Bud Neck 0.0004 0.0002 0 0.0001 0.0027 0.0008 0.0014 0 0.0003 0.0001 0.0004 0.0008 0.0005 0 0 0.0003 0.0001 0.0007
Bud Periphery 0.0003 0.0002 0 0 0.0003 0.0002 0.001 0 0.0003 0 0.0001 0.0005 0.0008 0 0.0002 0.0003 0 0.0001
Bud Site 0.0016 0.0048 0 0 0.0018 0.0001 0.002 0 0.0005 0 0 0.0002 0.0057 0 0.0004 0.0029 0 0
Cell Periphery 0.0001 0.0003 0 0 0.0001 0 0.0002 0 0.0001 0 0 0.0001 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0024 0.0005 0.0004 0 0.0004 0.0004 0.0075 0 0.0017 0.0011 0.0008 0.0003 0.0232 0.0001 0.001 0.0035 0.0001 0.0015
Cytoplasmic Foci 0.0045 0 0 0 0.0018 0.0001 0.0124 0 0.004 0.0006 0.0003 0.0018 0.0136 0 0.001 0.0053 0 0
Eisosomes 0.0001 0 0 0 0.0003 0 0.0002 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0011 0 0 0 0.0005 0.0003 0.0031 0 0.0014 0.0003 0.0003 0.0004 0.0021 0 0.0003 0.0009 0 0
Endosome 0.0062 0 0 0 0.0038 0.0008 0.0122 0 0.007 0.0009 0.0029 0.0042 0.0058 0 0.0009 0.0089 0 0
Golgi 0.001 0 0 0 0.0018 0.0001 0.003 0 0.0021 0.0007 0.0002 0.0016 0.0014 0 0.0004 0.0014 0 0
Lipid Particles 0.004 0 0 0 0.0039 0.0001 0.012 0 0.0028 0.0219 0.0001 0.0036 0.0075 0 0.0001 0.0022 0 0
Mitochondria 0.0012 0.0001 0.0001 0.0001 0.0009 0.0004 0.0055 0.0001 0.0075 0.0009 0.0009 0.0042 0.0031 0.0002 0.0024 0.003 0.0001 0.0002
None 0.0041 0.0001 0.0013 0 0.0014 0.0024 0.0041 0 0.0008 0.0032 0.0003 0.0003 0.0067 0.0001 0.0006 0.0028 0 0
Nuclear Periphery 0.0094 0.0031 0.0021 0.0012 0.0057 0.0042 0.0236 0.0031 0.0115 0.017 0.0119 0.0052 0.0082 0.0013 0.0017 0.0098 0.0005 0.001
Nucleolus 0.0153 0.019 0.0093 0.0118 0.0292 0.0213 0.0129 0.0078 0.0064 0.005 0.0229 0.0187 0.0148 0.0066 0.0112 0.0106 0.0045 0.0164
Nucleus 0.9304 0.9695 0.9847 0.9864 0.9119 0.9623 0.8617 0.9871 0.9385 0.933 0.9544 0.9474 0.878 0.9911 0.9671 0.9169 0.9945 0.9794
Peroxisomes 0.0015 0 0 0 0.0013 0 0.0094 0 0.0017 0.0007 0 0.0022 0.0058 0 0.0009 0.0009 0 0
Punctate Nuclear 0.0087 0.0009 0.0016 0.0003 0.0194 0.0037 0.0101 0.0017 0.0051 0.0134 0.0033 0.0026 0.0188 0.0002 0.0004 0.026 0.0001 0.0002
Vacuole 0.0032 0.0008 0.0002 0 0.0005 0.0005 0.0029 0 0.0012 0.0003 0.0004 0.0005 0.0016 0.0001 0.0002 0.0015 0 0.0001
Vacuole Periphery 0.0012 0.0001 0.0001 0 0.0004 0.0002 0.0018 0.0001 0.0015 0.0007 0.0005 0.0005 0.0009 0.0001 0.0002 0.0008 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.3211 35.1946 22.6796 33.323 34.2541 42.455 31.6053 28.758 29.0331 45.608
Translational Efficiency 1.1949 1.0807 1.4309 1.0498 1.38 1.4876 1.2266 1.1834 1.2567 1.053

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rfc5

Rfc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available